Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,042,337	G→C	5.7%	E92Q (GAA→CAA) ‡	PP_5295 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,042,337	0	G	C	5.7%	276.9 / 5.8	87	E92Q (GAA→CAA) ‡	PP_5295	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (41/41);  new base C (2/3);  total (43/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.42e-01

CCGGCAGCCAAGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCTGTCAT  >  NC_002947/6042191‑6042484                                                                                                                                                    |                                                                                                                                                    ccGGCAGCCAAGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGaa                                                                                                                                                   <  5:369596/149‑1 (MQ=255)   ggCAGCCAAGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATg                                                                                                                                                 <  5:319868/149‑1 (MQ=255)      aGCCAAGGCACCCTGGTCTGGGAGCTGCGTTCTGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCAc                                                                                                                                              <  7:304424/149‑1 (MQ=255)        ccAAGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCt                                                                                                                                            >  3:74117/1‑149 (MQ=255)        ccAAGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCt                                                                                                                                            >  3:73278/1‑149 (MQ=255)          aaGGCACCCTGGTCTGGGAGCGGCGGTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGCATTCCACCTTg                                                                                                                                          <  8:25869/149‑1 (MQ=255)           aGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTgc                                                                                                                                         <  1:90720/149‑1 (MQ=255)            ggCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGCATTCCACCTTgcc                                                                                                                                        <  4:150516/149‑2 (MQ=255)                  cTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCaa                                                                                                                                  <  5:134367/149‑1 (MQ=255)                  cTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCaa                                                                                                                                  <  8:253152/149‑1 (MQ=255)                   tGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAAc                                                                                                                                 >  3:50574/1‑149 (MQ=255)                     gTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAAc                                                                                                                                 <  1:266340/147‑1 (MQ=255)                          ggAGCTGCGTTCGGTCAACCTCCGTTATCTGGAGCCGCAGCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGAc                                                                                                                           <  5:216132/148‑1 (MQ=255)                               tGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAAt                                                                                                                     >  3:178663/1‑149 (MQ=255)                                 cGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATgg                                                                                                                   <  4:73278/149‑1 (MQ=255)                                 cGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATgg                                                                                                                   <  6:314026/149‑1 (MQ=255)                                  gatCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGCCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAAAgg                                                                                                                   >  2:143891/3‑148 (MQ=255)                                     cGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGaa                                                                                                                              >  5:141319/1‑134 (MQ=255)                                     cGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGaa                                                                                                                              <  6:141319/134‑1 (MQ=255)                                          aaCCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCt                                                                                                          >  5:60772/1‑149 (MQ=255)                                           accaccGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTgc                                                                                                                                         <  4:280096/117‑1 (MQ=255)                                           accaccGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTgc                                                                                                                                         >  3:280096/1‑117 (MQ=255)                                               ccGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAg                                                                                                     >  5:198357/1‑149 (MQ=255)                                                     tCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGAc                                                                                                <  3:287562/148‑1 (MQ=255)                                                       aggAGCCCCACCTGCGCCTGCCCGAAGCCCCGCGCGACCTCGACGGCCGGGGACGTGAAGCCCCGCG‑CCAGGGCCTGTCACGCGGCAAGGCGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCAt                                                                                             <  4:256130/148‑1 (MQ=255)                                                        ggAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCAt                                                                                             >  6:111254/1‑148 (MQ=255)                                                         gAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCAt                                                                                             <  4:205851/147‑1 (MQ=255)                                                            ccGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAgcg                                                                                        >  4:152194/1‑149 (MQ=255)                                                              gCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAgcgcg                                                                                      >  7:154117/1‑149 (MQ=255)                                                              gCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAgcgcg                                                                                      >  2:226137/1‑149 (MQ=255)                                                                aCCTGCGCCTGCCCGACGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGTCCATGAGcgcgcc                                                                                    <  4:77633/149‑1 (MQ=255)                                                                  cTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACAATGAGcgcgccgc                                                                                  >  4:35819/1‑149 (MQ=255)                                                                      gcCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCa                                                                               >  2:73439/1‑148 (MQ=255)                                                                        cTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCaa                                                                                                                      <  3:254719/107‑1 (MQ=255)                                                                        cTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCaa                                                                                                                      >  4:254719/1‑107 (MQ=255)                                                                          gCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTgg                                                                          >  8:32335/1‑149 (MQ=255)                                                                             cGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTg                                                                       >  2:158033/1‑149 (MQ=255)                                                                             cGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTg                                                                       <  4:74117/149‑1 (MQ=255)                                                                             cGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTg                                                                       >  4:194452/1‑149 (MQ=255)                                                                               aaGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACt                                                                            <  1:68122/142‑1 (MQ=255)                                                                               aaGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACt                                                                            >  2:68122/1‑142 (MQ=255)                                                                                  cccTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTgccgcc                                                                  >  3:321220/1‑149 (MQ=255)                                                                                      gcgcGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCa                                                                                                                                               >  8:203032/1‑68 (MQ=255)                                                                                      gcgcGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCa                                                                                                                                               <  7:203032/68‑1 (MQ=255)                                                                                         acgcccagAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGACGCGCAACTGGTTGCCGCCGCCGAgg                                                           <  1:320351/142‑1 (MQ=255)                                                                                             cTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGt                                                       >  6:358901/1‑149 (MQ=255)                                                                                              tCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAg                                                                                          >  8:248465/1‑113 (MQ=255)                                                                                              tCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAg                                                                                          <  7:248465/113‑1 (MQ=255)                                                                                               cGAAGGCGGTGTACGTGAAGGCCTGCGTCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACATTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCaa                                                                              >  1:17130/1‑125 (MQ=255)                                                                                               cGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCaa                                                                              <  2:17130/124‑1 (MQ=255)                                                                                               cGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGtgg                                                     >  2:18768/1‑149 (MQ=255)                                                                                                gAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCCCAACTGGTTGCCGCCGCCGAGGAAGtgg                                                     <  1:208285/148‑1 (MQ=255)                                                                                                    gCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGtggctggc                                                <  4:50574/149‑1 (MQ=255)                                                                                                     cGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGcc                                               <  1:158033/149‑1 (MQ=255)                                                                                                     cGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGcc                                               <  1:73439/149‑1 (MQ=255)                                                                                                          tACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGAt                                           <  2:190927/148‑1 (MQ=255)                                                                                                               gAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAag                                       >  2:175002/1‑147 (MQ=255)                                                                                                                    ccTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAAAGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCgg                                >  6:218005/1‑149 (MQ=255)                                                                                                                     cTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGc                               >  7:160670/1‑149 (MQ=255)                                                                                                                     cTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGc                               >  5:56808/1‑149 (MQ=255)                                                                                                                     cTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGc                               >  5:51280/1‑149 (MQ=255)                                                                                                                      tGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGcc                              >  4:243750/1‑149 (MQ=255)                                                                                                                               ggCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGcccc                            <  4:176577/143‑1 (MQ=255)                                                                                                                               ggCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGcccc                            >  3:176577/1‑143 (MQ=255)                                                                                                                                 ccTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAAcc                    <  3:194452/149‑1 (MQ=255)                                                                                                                                   tGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCg                  >  6:283159/1‑149 (MQ=255)                                                                                                                                     tCACTCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGACAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGc                 <  4:70185/148‑1 (MQ=255)                                                                                                                                     tCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGc                 <  4:178663/148‑1 (MQ=255)                                                                                                                                     tCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGc                 <  3:152194/148‑1 (MQ=255)                                                                                                                                     tCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCt                >  5:45804/1‑149 (MQ=255)                                                                                                                                       aCGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTg                                                                                                   >  6:326569/1‑64 (MQ=255)                                                                                                                                       aCGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTg                                                                                                   <  5:326569/64‑1 (MQ=255)                                                                                                                                       aCGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTg               >  3:267315/1‑148 (MQ=255)                                                                                                                                       aCGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTg               >  4:183294/1‑148 (MQ=255)                                                                                                                                         gcgGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGaa            <  1:175002/149‑1 (MQ=255)                                                                                                                                           ggCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGt          >  5:23263/1‑149 (MQ=255)                                                                                                                                           ggCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGt          >  2:129257/1‑149 (MQ=255)                                                                                                                                            gCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTg         <  1:18768/149‑1 (MQ=255)                                                                                                                                            gCAAGGTGCATTCCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTgg              >  2:242948/1‑144 (MQ=255)                                                                                                                                            gCAAGGTGCATTCCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTgg              <  1:242948/144‑1 (MQ=255)                                                                                                                                            gCAAGGTGCATTCCACCTTGCGCCACAACGAAGACAGCAATGGCAAACCGCTTAAAGTTGACCATGCGCGCGCCGCGCAACTGGTTGCAGCCGCCGAGGAAGTGGCTGGCCTCATCAAGCAGCCGGCCCCCATGAACCCGCTGGaatt          >  7:54495/1‑146 (MQ=255)                                                                                                                                              aaGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCATACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCt       <  4:267315/149‑1 (MQ=255)                                                                                                                                              aaGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCt       <  6:45804/149‑1 (MQ=255)                                                                                                                                               cggTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGGGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCt       <  7:279991/147‑1 (MQ=255)                                                                                                                                               aGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCCGCCGGCCCCCCTGAACCCGCTGGAAggcct       >  8:279991/1‑144 (MQ=255)                                                                                                                                               aGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCTg      <  6:198357/149‑1 (MQ=255)                                                                                                                                                 gTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCTGTc    <  5:283159/149‑1 (MQ=255)                                                                                                                                                   ggAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCTGTCAt  >  4:44302/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CCGGCAGCCAAGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCTGTCAT  >  NC_002947/6042191‑6042484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑