Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,042,341	G→C	5.7%	C93S (TGC→TCC)	PP_5295 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,042,341	0	G	C	5.7%	284.0 / 5.7	87	C93S (TGC→TCC)	PP_5295	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (43/39);  new base C (2/3);  total (45/42)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.08e-01

GGCAGCCAAGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCTGTCATGGC  >  NC_002947/6042193‑6042487                                                                                                                                                      |                                                                                                                                                   ggCAGCCAAGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATg                                                                                                                                                    <  5:319868/149‑1 (MQ=255)    aGCCAAGGCACCCTGGTCTGGGAGCTGCGTTCTGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCAc                                                                                                                                                 <  7:304424/149‑1 (MQ=255)      ccAAGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCt                                                                                                                                               >  3:74117/1‑149 (MQ=255)      ccAAGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCt                                                                                                                                               >  3:73278/1‑149 (MQ=255)        aaGGCACCCTGGTCTGGGAGCGGCGGTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGCATTCCACCTTg                                                                                                                                             <  8:25869/149‑1 (MQ=255)         aGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTgc                                                                                                                                            <  1:90720/149‑1 (MQ=255)          ggCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGCATTCCACCTTgcc                                                                                                                                           <  4:150516/149‑2 (MQ=255)                cTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCaa                                                                                                                                     <  8:253152/149‑1 (MQ=255)                cTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCaa                                                                                                                                     <  5:134367/149‑1 (MQ=255)                 tGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAAc                                                                                                                                    >  3:50574/1‑149 (MQ=255)                   gTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAAc                                                                                                                                    <  1:266340/147‑1 (MQ=255)                        ggAGCTGCGTTCGGTCAACCTCCGTTATCTGGAGCCGCAGCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGAc                                                                                                                              <  5:216132/148‑1 (MQ=255)                             tGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAAt                                                                                                                        >  3:178663/1‑149 (MQ=255)                               cGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATgg                                                                                                                      <  4:73278/149‑1 (MQ=255)                               cGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATgg                                                                                                                      <  6:314026/149‑1 (MQ=255)                                gatCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGCCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAAAgg                                                                                                                      >  2:143891/3‑148 (MQ=255)                                   cGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGaa                                                                                                                                 >  5:141319/1‑134 (MQ=255)                                   cGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGaa                                                                                                                                 <  6:141319/134‑1 (MQ=255)                                        aaCCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCt                                                                                                             >  5:60772/1‑149 (MQ=255)                                         accaccGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTgc                                                                                                                                            >  3:280096/1‑117 (MQ=255)                                         accaccGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTgc                                                                                                                                            <  4:280096/117‑1 (MQ=255)                                             ccGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAg                                                                                                        >  5:198357/1‑149 (MQ=255)                                                   tCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGAc                                                                                                   <  3:287562/148‑1 (MQ=255)                                                     aggAGCCCCACCTGCGCCTGCCCGAAGCCCCGCGCGACCTCGACGGCCGGGGACGTGAAGCCCCGCG‑CCAGGGCCTGTCACGCGGCAAGGCGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCAt                                                                                                <  4:256130/148‑1 (MQ=255)                                                      ggAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCAt                                                                                                >  6:111254/1‑148 (MQ=255)                                                       gAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCAt                                                                                                <  4:205851/147‑1 (MQ=255)                                                          ccGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAgcg                                                                                           >  4:152194/1‑149 (MQ=255)                                                            gCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAgcgcg                                                                                         >  2:226137/1‑149 (MQ=255)                                                            gCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAgcgcg                                                                                         >  7:154117/1‑149 (MQ=255)                                                              aCCTGCGCCTGCCCGACGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGTCCATGAGcgcgcc                                                                                       <  4:77633/149‑1 (MQ=255)                                                                cTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACAATGAGcgcgccgc                                                                                     >  4:35819/1‑149 (MQ=255)                                                                    gcCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCa                                                                                  >  2:73439/1‑148 (MQ=255)                                                                      cTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCaa                                                                                                                         <  3:254719/107‑1 (MQ=255)                                                                      cTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCaa                                                                                                                         >  4:254719/1‑107 (MQ=255)                                                                        gCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTgg                                                                             >  8:32335/1‑149 (MQ=255)                                                                           cGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTg                                                                          >  4:194452/1‑149 (MQ=255)                                                                           cGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTg                                                                          <  4:74117/149‑1 (MQ=255)                                                                           cGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTg                                                                          >  2:158033/1‑149 (MQ=255)                                                                             aaGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACt                                                                               <  1:68122/142‑1 (MQ=255)                                                                             aaGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACt                                                                               >  2:68122/1‑142 (MQ=255)                                                                                cccTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTgccgcc                                                                     >  3:321220/1‑149 (MQ=255)                                                                                    gcgcGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCa                                                                                                                                                  <  7:203032/68‑1 (MQ=255)                                                                                    gcgcGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCa                                                                                                                                                  >  8:203032/1‑68 (MQ=255)                                                                                       acgcccagAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGACGCGCAACTGGTTGCCGCCGCCGAgg                                                              <  1:320351/142‑1 (MQ=255)                                                                                           cTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGt                                                          >  6:358901/1‑149 (MQ=255)                                                                                            tCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAg                                                                                             >  8:248465/1‑113 (MQ=255)                                                                                            tCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAg                                                                                             <  7:248465/113‑1 (MQ=255)                                                                                             cGAAGGCGGTGTACGTGAAGGCCTGCGTCCAGGGCCTGTCACGCGGCAAGGTGGAATGCACATTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCaa                                                                                 >  1:17130/1‑125 (MQ=255)                                                                                             cGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCaa                                                                                 <  2:17130/124‑1 (MQ=255)                                                                                             cGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGtgg                                                        >  2:18768/1‑149 (MQ=255)                                                                                              gAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCCCAACTGGTTGCCGCCGCCGAGGAAGtgg                                                        <  1:208285/148‑1 (MQ=255)                                                                                                  gCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGtggctggc                                                   <  4:50574/149‑1 (MQ=255)                                                                                                   cGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGcc                                                  <  1:73439/149‑1 (MQ=255)                                                                                                   cGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGcc                                                  <  1:158033/149‑1 (MQ=255)                                                                                                        tACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGAt                                              <  2:190927/148‑1 (MQ=255)                                                                                                             gAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAag                                          >  2:175002/1‑147 (MQ=255)                                                                                                                  ccTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAAAGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCgg                                   >  6:218005/1‑149 (MQ=255)                                                                                                                   cTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGc                                  >  7:160670/1‑149 (MQ=255)                                                                                                                   cTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGc                                  >  5:51280/1‑149 (MQ=255)                                                                                                                   cTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGc                                  >  5:56808/1‑149 (MQ=255)                                                                                                                    tGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGcc                                 >  4:243750/1‑149 (MQ=255)                                                                                                                             ggCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGcccc                               >  3:176577/1‑143 (MQ=255)                                                                                                                             ggCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGcccc                               <  4:176577/143‑1 (MQ=255)                                                                                                                               ccTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAAcc                       <  3:194452/149‑1 (MQ=255)                                                                                                                                 tGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCg                     >  6:283159/1‑149 (MQ=255)                                                                                                                                   tCACTCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGACAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGc                    <  4:70185/148‑1 (MQ=255)                                                                                                                                   tCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGc                    <  3:152194/148‑1 (MQ=255)                                                                                                                                   tCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGc                    <  4:178663/148‑1 (MQ=255)                                                                                                                                   tCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCt                   >  5:45804/1‑149 (MQ=255)                                                                                                                                     aCGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTg                                                                                                      <  5:326569/64‑1 (MQ=255)                                                                                                                                     aCGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTg                                                                                                      >  6:326569/1‑64 (MQ=255)                                                                                                                                     aCGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTg                  >  3:267315/1‑148 (MQ=255)                                                                                                                                     aCGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTg                  >  4:183294/1‑148 (MQ=255)                                                                                                                                       gcgGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGaa               <  1:175002/149‑1 (MQ=255)                                                                                                                                         ggCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGt             >  2:129257/1‑149 (MQ=255)                                                                                                                                         ggCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGt             >  5:23263/1‑149 (MQ=255)                                                                                                                                          gCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTg            <  1:18768/149‑1 (MQ=255)                                                                                                                                          gCAAGGTGCATTCCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTgg                 >  2:242948/1‑144 (MQ=255)                                                                                                                                          gCAAGGTGCATTCCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTgg                 <  1:242948/144‑1 (MQ=255)                                                                                                                                          gCAAGGTGCATTCCACCTTGCGCCACAACGAAGACAGCAATGGCAAACCGCTTAAAGTTGACCATGCGCGCGCCGCGCAACTGGTTGCAGCCGCCGAGGAAGTGGCTGGCCTCATCAAGCAGCCGGCCCCCATGAACCCGCTGGaatt             >  7:54495/1‑146 (MQ=255)                                                                                                                                            aaGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCATACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCt          <  4:267315/149‑1 (MQ=255)                                                                                                                                            aaGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCt          <  6:45804/149‑1 (MQ=255)                                                                                                                                             cggTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGGGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCt          <  7:279991/147‑1 (MQ=255)                                                                                                                                             aGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCCGCCGGCCCCCCTGAACCCGCTGGAAggcct          >  8:279991/1‑144 (MQ=255)                                                                                                                                             aGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCTg         <  6:198357/149‑1 (MQ=255)                                                                                                                                               gTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCTGTc       <  5:283159/149‑1 (MQ=255)                                                                                                                                                 ggAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCTGTCAt     >  4:44302/1‑149 (MQ=255)                                                                                                                                                    aTGCACCTTGCGCCTCAACGAAGACAGCAATGGCCAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAGGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCGGTCATgcc  >  2:346065/1‑147 (MQ=255)                                                                                                                                                      |                                                                                                                                                   GGCAGCCAAGGCACCCTGGTCTGGGAGCTGCGTTCGGTCAACCACCGTTATCTGGAGCCGCACCTGCGCCTGCCCGAAGCCCTGCGCGACCTCGAAGGCGGTGTACGTGAAGGCCTGCG‑CCAGGGCCTGTCACGCGGCAAGGTGGAATGCACCTTGCGCCTCAACGAAGACAGCAATGGCAAACCGCTGAAAGTGGACCATGAGCGCGCCGCGCAACTGGTTGCCGCCGCCGAGGAAGTGGCTGGCCTGATCAAGCAGCCGGCCCCCCTGAACCCGCTGGAAGTGCTGTCATGGC  >  NC_002947/6042193‑6042487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑