Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,079,438 A→C 5.6% intergenic (‑213/‑149) PP_5330 ← / → PP_5331 MFS transporter/acyl‑CoA thioesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,079,4380AC 5.6% 280.8 / 6.2 89intergenic (‑213/‑149)PP_5330/PP_5331MFS transporter/acyl‑CoA thioesterase
Reads supporting (aligned to +/- strand):  ref base A (42/42);  new base C (2/3);  total (44/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.98e-01

GCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATC  >  NC_002947/6079290‑6079584
                                                                                                                                                    |                                                                                                                                                  
gCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGaaaa                                                                                                                                                    >  1:245643/1‑149 (MQ=255)
   aaGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTGCTAACGTGGGATGTTGTGCGAAAACGc                                                                                                                                                 >  8:285992/1‑149 (MQ=255)
   aaGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGc                                                                                                                                                 >  4:307671/1‑149 (MQ=255)
       tAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTTACGTGGGATGTTGTTAGTAAACGACgg                                                                                                                                              >  3:280820/1‑148 (MQ=255)
          cAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCAc                                                                                                                                          <  8:76323/149‑1 (MQ=255)
          cAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCAc                                                                                                                                          >  1:295987/1‑149 (MQ=255)
           aaaTTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCAc                                                                                                                                          >  5:235112/1‑148 (MQ=255)
            aaTTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTa                                                                                                                                        >  1:197513/1‑149 (MQ=255)
                   gAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAg                                                                                                                                 <  4:216754/149‑1 (MQ=255)
                   gAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAg                                                                                                                                 <  6:76297/149‑1 (MQ=255)
                    aGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAgg                                                                                                                                >  3:15679/1‑149 (MQ=255)
                      cTACGCCGAAAGCGATACAAAATCCCCACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTCTGGCCTATATACTGGCGACTCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCg                                                                                                                              <  7:253696/149‑1 (MQ=255)
                      cTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCg                                                                                                                              <  1:276937/149‑1 (MQ=255)
                        aCGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAACCGCCTGACAATAGTGACCGGCgtt                                                                                                                            >  5:188313/1‑147 (MQ=255)
                        aCGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGt                                                                                                                            >  1:215019/1‑149 (MQ=255)
                         cGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCgg                                                                                                                             <  4:15679/147‑1 (MQ=255)
                         cGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGGGCGAAAACGCCGGTCACTATTGTCAGGCgg                                                                                                                             <  1:51050/147‑1 (MQ=255)
                             gAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAACCGCCTGACAATAGTGACCGGCGTTTTCGc                                                                                                                       <  6:188313/149‑1 (MQ=38)
                              aaaGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAACCGCCTGACAATAGTGACCGGCGTTTTCGCa                                                                                                                      <  6:171519/149‑1 (MQ=38)
                              aaaGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCg                                                                                                                        >  7:232419/1‑147 (MQ=255)
                              aaaGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCa                                                                                                                      <  8:232419/149‑1 (MQ=255)
                              aaaGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCa                                                                                                                      <  6:235112/149‑1 (MQ=255)
                               aaGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAACCGCCTGACAATAGTGACCGGCGTTTTCGCa                                                                                                                      <  8:132384/148‑1 (MQ=38)
                                aGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCaga                                                                                                                    <  4:191449/149‑1 (MQ=255)
                                    aTACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCCCATGCCGTTATGCCCTATATCCTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGttt                                                                                                                <  8:173944/149‑1 (MQ=255)
                                     tACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTc                                                                                                               <  2:215019/149‑1 (MQ=255)
                                     tACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTc                                                                                                               >  4:150149/1‑149 (MQ=255)
                                     tACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTc                                                                                                               >  6:33129/1‑149 (MQ=255)
                                      aCAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTcc                                                                                                              >  2:101125/1‑149 (MQ=255)
                                       cAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTccc                                                                                                             >  3:318785/1‑149 (MQ=255)
                                       cAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTccc                                                                                                             <  7:113867/149‑1 (MQ=255)
                                       cAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTcc                                                                                                              <  2:287319/148‑1 (MQ=255)
                                        aaaaTCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCt                                                                                                            <  8:136954/149‑1 (MQ=255)
                                            tCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACgg                                                                                                        <  3:91460/149‑1 (MQ=255)
                                               acacaGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGgccg                                                                                                     >  5:304326/1‑149 (MQ=255)
                                                 acaGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGgccgcc                                                                                                   >  1:200617/1‑149 (MQ=255)
                                                 acaGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGgccgcc                                                                                                   >  2:42407/1‑149 (MQ=255)
                                                  caGATATTCGTGAATGCGCGGTCGTCGCTGTCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTACTAACGTGGGATGTTGTGCGAAAACGCAGGTCAATATTGTCAGGCGGTTTCTCAGAGTTTCCCTACgaagatt                                                                                                   >  8:218210/1‑142 (MQ=255)
                                                   aGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAAGGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACTGCCGccct                                                                                                 >  8:263471/1‑148 (MQ=255)
                                                   aGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGccct                                                                                                 >  6:15699/1‑148 (MQ=255)
                                                       atTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTg                                                                                              >  6:181599/1‑148 (MQ=255)
                                                           gTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAAc                                                                                          <  2:197513/148‑1 (MQ=255)
                                                              aaTGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGaa                                                                                       >  4:274426/1‑148 (MQ=255)
                                                              aaTGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGCATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGaaa                                                                                      >  8:258500/1‑149 (MQ=255)
                                                                  cgcgGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTTGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCg                                                                                   <  7:120030/148‑1 (MQ=255)
                                                                   gcgGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGgccgc                                                                                                    <  7:89961/130‑1 (MQ=255)
                                                                   gcgGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGgccgc                                                                                                    >  8:89961/1‑130 (MQ=255)
                                                                    cgGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGCATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCg                                                                                <  7:258500/149‑1 (MQ=255)
                                                                      gtcgtcGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCgta                                                                              <  5:276223/149‑1 (MQ=255)
                                                                      gtcgtcGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCgta                                                                              <  5:85126/149‑1 (MQ=255)
                                                                         gtcgCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCgtagta                                                                           >  3:308926/1‑149 (MQ=255)
                                                                          tcgCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTAt                                                                          >  2:180307/1‑149 (MQ=255)
                                                                             cTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGtt                                                                       <  1:180307/149‑1 (MQ=255)
                                                                                 ccGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCaa                                                                   <  2:245643/149‑1 (MQ=255)
                                                                                 ccGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCaa                                                                   <  1:101125/149‑1 (MQ=255)
                                                                                 ccGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCaa                                                                   <  2:295987/149‑1 (MQ=255)
                                                                                         cccTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAAt                                                            <  2:200617/148‑1 (MQ=255)
                                                                                          ccTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCg                                                          >  5:74598/1‑149 (MQ=255)
                                                                                          ccTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCg                                                          >  7:137061/1‑149 (MQ=255)
                                                                                            tatataCTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGt                                                                                                                                             >  1:2156/1‑64 (MQ=255)
                                                                                            tatataCTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGt                                                                                                                                             <  2:2156/64‑1 (MQ=255)
                                                                                              tataCTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGttt                                                       <  5:181599/148‑1 (MQ=255)
                                                                                              tataCTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTa                                                      <  1:42407/149‑1 (MQ=255)
                                                                                                 aCTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTAccc                                                   >  3:152557/1‑149 (MQ=255)
                                                                                                 aCTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTAccc                                                   >  1:309503/1‑149 (MQ=255)
                                                                                                  cTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTAccc                                                   <  3:150149/148‑1 (MQ=255)
                                                                                                  cTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCg                                                  <  3:274426/149‑1 (MQ=255)
                                                                                                   tGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCgg                                                 >  6:48943/1‑149 (MQ=255)
                                                                                                   tGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCgg                                                 >  4:56924/1‑149 (MQ=255)
                                                                                                     gCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCgg                                                 <  8:137061/147‑1 (MQ=255)
                                                                                                       gAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCGGCCtt                                             >  8:279153/1‑149 (MQ=255)
                                                                                                          tCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCGGCCTTTgc                                          <  3:56924/149‑1 (MQ=255)
                                                                                                          tCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCGGCCTTTgc                                          <  7:285992/149‑1 (MQ=255)
                                                                                                            gTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCGGCCTTTgcgc                                        <  3:307671/149‑1 (MQ=255)
                                                                                                             tAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCaa                                                                   <  1:47071/121‑1 (MQ=255)
                                                                                                             tAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCaa                                                                   >  2:47071/1‑121 (MQ=255)
                                                                                                                     tGTTGCTTCTAACGTGGGTTGTTTTGCGAAAACGCCCGTCACCATTTTGACGGGGTTTCGGATTTGTTCCCTAAGGCCGGCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCGGCCTTTGCGCCACACCAAc                               <  5:15699/149‑1 (MQ=255)
                                                                                                                     tGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCGGCCTTTGCGCCACACCaa                                <  5:48943/148‑1 (MQ=255)
                                                                                                                     tGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCGGCCTTTGCGCCACACCAAc                               <  4:308926/149‑1 (MQ=255)
                                                                                                                      gTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCGGCCTTTGCGCCaaacaaac                               >  5:259763/1‑148 (MQ=255)
                                                                                                                      gTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCGGCCTTTGCGCCACACCAAc                               >  4:308810/1‑148 (MQ=255)
                                                                                                                       ttGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCGGCCTTTGCGCCACACCAACg                              >  1:12491/1‑148 (MQ=255)
                                                                                                                         gCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCCAGCTGAATCGTTTAGCCGGGCTTTGCGCCACACCAACGaa                            >  6:292422/1‑148 (MQ=255)
                                                                                                                            ccTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCTTAGTATGTTTCAGGCTGAATCGTTTACCCGGGCATTGCGCCACACCAACGAAAAGc                        >  2:324328/1‑149 (MQ=255)
                                                                                                                              tAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCGGCCTTTGCGCcaca                                    <  2:246886/135‑1 (MQ=255)
                                                                                                                              tAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCGGCCTTTGCGCcaca                                    >  1:246886/1‑135 (MQ=255)
                                                                                                                              tAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCGGCCTTTGCGCCACACCAACGAAAAGCGt                      >  5:94681/1‑149 (MQ=255)
                                                                                                                                aCGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCGGCCTTTGCGCCACACCAACGAAAAGCGt                      <  7:279153/147‑1 (MQ=255)
                                                                                                                                              gCGAAACCGCCTGACAATAGTGACCGGCGTTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGccc      >  8:225248/1‑149 (MQ=38)
                                                                                                                                                gAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTAAGTTTCAAGCTGAATCGTTTACCCGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCa    >  5:90082/1‑149 (MQ=255)
                                                                                                                                                 aaaaCGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCAt   >  1:203905/1‑149 (MQ=255)
                                                                                                                                                  aaaCGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCGGCCTTTGCGCCACACCAACGAAAAGCGTCACGTGTCAGAGGCCCCATc  <  6:304326/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GCGAAGATAGCAAATTGCCGAGCTACGCCGAAAGCGATACAAAATCCACACAGATATTCGTGAATGCGCGGTCGTCGCTGGCCGTTATGCCCTATATACTGGCGAATCGTAGGATTATGTTGCTCCTAACGTGGGATGTTGTGCGAAAACGCCGGTCACTATTGTCAGGCGGTTTCGCAGAGTTTCCCTACGGCCGCCCAGTGAAACGAAATCGGCGTAGTATGTTTCAAGCTGAATCGTTTACCCGGCCTTTGCGCCACACCAACGAAAAGCGTCACGGGTCAGAGGCCCCATC  >  NC_002947/6079290‑6079584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑