BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,111,188	G→C	8.7%	P108A (CCA→GCA)	PP_5361 ←	protein P47K

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,111,188	0	G	C	8.7%	305.8 / 16.8	103	P108A (CCA→GCA)	PP_5361	protein P47K
Reads supporting (aligned to +/- strand): ref base G (44/49); new base C (5/4); total (49/54)
Fisher's exact test for biased strand distribution p-value = 7.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.59e-01

Only 100 of 104 total aligned reads displayed.

TCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGACCAGTTTCTCTTCAGCGCGG  >  NC_002947/6111043‑6111336

tCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCa                                                                                                                                                        <  2:39840/148‑1 (MQ=255)

   ccccGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTgg                                                                                                                                                    >  7:142188/1‑149 (MQ=255)

   ccccGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGCCAGTgg                                                                                                                                                    >  5:191268/1‑149 (MQ=255)

    cccGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGc                                                                                                                                                   >  3:147051/1‑149 (MQ=255)

      cGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGCCAGTGGCtg                                                                                                                                                 <  3:130061/149‑2 (MQ=255)

       ggCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCa                                                                                                                                                <  7:326411/149‑1 (MQ=255)

       ggCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCa                                                                                                                                                <  8:226534/149‑1 (MQ=255)

       ggCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGCCAGTGGCtga                                                                                                                                                <  6:191268/149‑3 (MQ=255)

        gCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGCCAGTGGCt                                                                                                                                                  >  8:308465/1‑146 (MQ=255)

         cTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCaga                                                                                                                                              <  5:22683/149‑1 (MQ=255)

         cTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCaga                                                                                                                                              <  5:346520/149‑1 (MQ=255)

         cTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCaga                                                                                                                                              <  5:257524/149‑1 (MQ=255)

         cTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGCCAGTGGCTgaga                                                                                                                                              <  7:308465/149‑1 (MQ=255)

            gCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCagag                                                                                                                                             <  5:361770/147‑1 (MQ=255)

             ccAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑a                                                                                                                                       <  2:51206/149‑1 (MQ=255)

               aGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTgg                                                                                                                                                    >  6:317267/1‑137 (MQ=255)

               aGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTgg                                                                                                                                                    <  5:317267/137‑1 (MQ=255)

                 gCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCgg                                                                                                                                   >  1:283501/1‑149 (MQ=255)

                  cTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGt                                                                                                                                  <  3:171730/149‑1 (MQ=255)

                  cTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGt                                                                                                                                  <  8:7168/149‑1 (MQ=255)

                  cTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGt                                                                                                                                  <  2:283501/149‑1 (MQ=255)

                  cTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGt                                                                                                                                  <  2:218707/149‑1 (MQ=255)

                  cTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGt                                                                                                                                  <  2:205707/149‑1 (MQ=255)

                  cTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGt                                                                                                                                  <  2:109269/149‑1 (MQ=255)

                  cTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGt                                                                                                                                  >  8:206002/1‑149 (MQ=255)

                  cTGTCTGCTGCCTGACAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCGCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGt                                                                                                                                  <  6:94154/149‑1 (MQ=255)

                         ctgcGTGATAGTCGCGCAGGAAGTTCAAGGCATCGACCACGGTGACCATGGTGTCGAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCg                                                                                                                           <  5:7233/149‑1 (MQ=255)

                         ctgcCTGATAGTCGCGCAGGAAGTTCAAGCNATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCag                                                                                                                                               >  1:31573/1‑132 (MQ=255)

                         ctgcCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCag                                                                                                                                               <  2:31573/132‑1 (MQ=255)

                         ctgcCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCag                                                                                                                                               <  2:25932/132‑1 (MQ=255)

                         ctgcCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCag                                                                                                                                               >  1:25932/1‑132 (MQ=255)

                            ccTGATAGTCGCGCAGGAAGTTCAATCCATCGACCACGGTGACCATGGAGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTCGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGaaaccat                                                                                                                         >  4:36342/1‑142 (MQ=38)

                            ccTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGAt                                                                                                                         >  4:359605/1‑148 (MQ=255)

                             cTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCa                                                                                                                       <  6:246352/149‑1 (MQ=255)

                             cTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCa                                                                                                                       <  2:69338/149‑1 (MQ=255)

                             cTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCa                                                                                                                       <  8:51693/149‑1 (MQ=255)

                              tGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAg                                                                                                                      >  3:329550/1‑149 (MQ=255)

                               gATAGGCGCGCAGGAAGCTCAAGCCCTCGACCACGGTGACCATGGTGTCCAGGCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGc                                                                                                                     <  8:164308/149‑1 (MQ=255)

                                    tCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑aat                                                                                                                >  5:65243/1‑148 (MQ=255)

                                      tgccAGGCAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑aataat                                                                                                             <  7:134600/146‑1 (MQ=255)

                                      gcgcAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑aataat                                                                                                             >  5:92560/1‑149 (MQ=255)

                                       cgcAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATc                                                                                                            <  5:215625/149‑1 (MQ=255)

                                        gcAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCg                                                                                                           <  7:206002/149‑1 (MQ=255)

                                         cAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGt                                                                                                                                  >  3:264750/1‑126 (MQ=255)

                                         cAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGt                                                                                                                                  <  4:264750/126‑1 (MQ=255)

                                            gAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAAc                                                                                                       >  2:271967/1‑149 (MQ=255)

                                            gAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAAc                                                                                                       >  7:98988/1‑149 (MQ=255)

                                                  cAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCct                                                                                                 >  1:257611/1‑149 (MQ=255)

                                                   aaGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑aataat                                                                                                             >  3:289596/1‑136 (MQ=255)

                                                   aaGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑aataat                                                                                                             <  4:289596/136‑1 (MQ=255)

                                                      ccATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTc                                                                                             <  1:40741/149‑1 (MQ=255)

                                                       cATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCg                                                                                            <  5:382662/149‑1 (MQ=255)

                                                         tCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGc                                                                                          <  3:359605/149‑1 (MQ=255)

                                                            aCCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAg                                                                                       >  3:99035/1‑149 (MQ=255)

                                                                       cATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAAcct                                                                            >  3:305835/1‑149 (MQ=255)

                                                                       cATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAAcct                                                                            >  2:298804/1‑149 (MQ=255)

                                                                         tGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCCCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAAcctcc                                                                          <  3:94008/149‑1 (MQ=255)

                                                                           gtgtCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACg                                                                                                      <  5:241188/119‑1 (MQ=255)

                                                                           gtgtCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACg                                                                                                      >  6:241188/1‑119 (MQ=255)

                                                                           gtgtCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACctcctc                                                                        <  8:142188/149‑1 (MQ=255)

                                                                           gtgtCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACctcctc                                                                        <  4:99035/149‑1 (MQ=255)

                                                                              tCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAg                                                                     >  7:314150/1‑149 (MQ=255)

                                                                               ccAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCACCCTCCTCGAg                                                                     >  4:30780/1‑148 (MQ=255)

                                                                                cAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGc                                                                    <  6:65243/148‑1 (MQ=255)

                                                                                       gcCATTTCGGACTGACTGCGACGCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTCGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCGAGTCGCGGAACCTCCTCGAGCAGGTCTTc                                                            <  3:36342/149‑1 (MQ=255)

                                                                                           tatCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTcgcg                                                         >  7:118148/1‑148 (MQ=255)

                                                                                             tCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCaa                                                      >  7:294589/1‑149 (MQ=255)

                                                                                               ggACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCACAACCTCCTCGACCAGGTCTTCGCGCAACg                                                    >  5:272379/1‑149 (MQ=255)

                                                                                                gACAGACTGCGACCCTGTTCGTCGCGCAAAGTGAAGGTCTCAGCCACTCGCACTGGCTCAGAGATAAGAGCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTACGGCCAGTCGCGCACCCTCCTCGAGCAGGTCTTCGCGCaa                                                      >  1:116239/1‑149 (MQ=255)

                                                                                                         cGACGCTGATCGTCGCGGAAAGTGAAGGTCTGAGCCACTGGCAGTTGCTCAGAGAT‑‑‑ACCGGTTGACTCGATCAGCA‑AATAATCGAATCGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCaaa                                           <  2:116239/148‑1 (MQ=255)

                                                                                                         cGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAAt                                          >  2:360598/1‑149 (MQ=255)

                                                                                                          gACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATg                                         >  3:342054/1‑149 (MQ=255)

                                                                                                          gACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATg                                         <  1:271967/149‑1 (MQ=255)

                                                                                                          gACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATg                                         >  2:252049/1‑149 (MQ=255)

                                                                                                             ccTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAg                                      >  8:57773/1‑149 (MQ=255)

                                                                                                             ccTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAg                                      >  6:12513/1‑149 (MQ=255)

                                                                                                                gCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCAGCCAGTCGCGCAAcctcct                                                                         <  1:79038/111‑1 (MQ=255)

                                                                                                                   cgtcgCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCg                                                                                            <  8:117974/89‑1 (MQ=255)

                                                                                                                   cgtcgCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCg                                                                                            >  7:117974/1‑89 (MQ=255)

                                                                                                                       gcgGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTc                             <  4:342054/148‑1 (MQ=255)

                                                                                                                       gcgGAAAGTGAAGGTCTCAGCCACTGGCAGGGACTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCACCAGCCGCGAGCCGGTCTTCGCGCAACGTGCCGCAAATGCAGCCGTTGcg                              >  5:211439/1‑146 (MQ=255)

                                                                                                                          gAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCAtct                         >  4:197058/1‑149 (MQ=255)

                                                                                                                              gTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGAc                     <  3:54654/149‑1 (MQ=255)

                                                                                                                              gTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGAc                     <  3:197058/149‑1 (MQ=255)

                                                                                                                                 aaGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCACAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGACCAg                  >  5:282363/1‑149 (MQ=255)

                                                                                                                                    gTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGACCAGttt               >  4:291235/1‑149 (MQ=255)

                                                                                                                                    gTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGACCAGttt               <  6:182730/149‑1 (MQ=255)

                                                                                                                                      ctcAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCAGAATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGACCAGTTtc              >  1:82111/1‑149 (MQ=255)

                                                                                                                                       tgaGCCACTGCCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAg                                                                  >  6:139626/3‑95 (MQ=255)

                                                                                                                                       tgaGCCACTGCCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAg                                                                  <  5:139626/93‑1 (MQ=255)

                                                                                                                                       tgaGCCACTGCCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGACCAGTTtctc            >  5:137650/3‑149 (MQ=255)

                                                                                                                                       tgaGCCACTGCCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGACCAGTTtctc            >  3:145968/3‑149 (MQ=255)

                                                                                                                                       tcAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGACCAGTTtctc            >  7:221128/1‑149 (MQ=255)

                                                                                                                                         aGCCACTGTCAGTGTCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCATGTCTTCGCTCAACGTGCAGCAAATGCTGCCGTTGCTCATCTCGACCAGTTTCTCtt          <  5:26603/149‑1 (MQ=255)

                                                                                                                                         aGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGACCAGTTTctct           <  8:118148/148‑1 (MQ=255)

                                                                                                                                         aGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGACCAGTTTCTCtt          <  2:82111/149‑1 (MQ=255)

                                                                                                                                         aGCCACAGGCAGTGGCTAAGAGAT‑‑‑ACCGGGGGCCTCGATCAGCA‑AATAATCGAAACGCCCCGGTTCGGCCAGTAGCGGAACCGCCACGAGCAGGTCTTCGCCCAACCTGCAGCAAATGACGCCGTTGGGCCTATCGACCACTTTCTCtt          >  2:198130/1‑149 (MQ=255)

                                                                                                                                           ccACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGACCAGTTTCTCtt          >  8:159915/1‑147 (MQ=255)

                                                                                                                                           ccACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGACCAGTTTCTCTTCa        <  4:329550/149‑1 (MQ=255)

                                                                                                                                                 gCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGACCAGTTTCTCTTCAGCGCgg  <  2:257611/149‑1 (MQ=255)

TCGCCCCGGCTGGCCAGGCTGTCTGCTGCCTGATAGTCGCGCAGGAAGTTCAAGCCATCGACCACGGTGACCATGGTGTCCAGTCGCGCCATATCGGACAGACTGCGACCCTGCTCGTCGCGGAAAGTGAAGGTCTCAGCCACTGGCAGTGGCTCAGAGAT‑‑‑ACCGGTGGACTCGATCAGCA‑AATAATCGAAACGCCCCTCTTCGGCCAGTCGCGCAACCTCCTCGAGCAGGTCTTCGCGCAACGTGCAGCAAATGCAGCCGTTGCTCATCTCGACCAGTTTCTCTTCAGCGCGG  >  NC_002947/6111043‑6111336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`