Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,127,162 C→G 6.7% V241V (GTG→GTC) ‡ PP_5375 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,127,1620CG 6.7% 268.2 / 6.9 87V241V (GTG→GTC) ‡PP_5375LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (40/41);  new base G (3/3);  total (43/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.22e-01

AGCCGCACGCTTTCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTCAAGCCAGAGC  >  NC_002947/6127019‑6127309
                                                                                                                                                |                                                                                                                                                    
aGCCGCACGCTTTCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGcc                                                                                                                                                 <  5:68989/149‑1 (MQ=255)
  ccGCACGCTTTCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCt                                                                                                                                               >  3:176385/1‑149 (MQ=255)
  ccGCACGCTTTCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCt                                                                                                                                               <  6:161538/149‑1 (MQ=255)
     cACGCTTTCACGAGGACCACGCTCGGTGGTCGACTCCAGCAGGCAGAACTTGGCGTCGGT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCGTGGCCCTGGc                                                                                                                                            <  8:327395/149‑1 (MQ=255)
     cACGCTTTCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGACCCAGGCCAGGGCCACGCCCTgg                                                                                                                                             >  5:74055/1‑148 (MQ=255)
         cTTTCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCGTGGCCCTGGcctg                                                                                                                                         <  3:138805/148‑3 (MQ=255)
         cTTTCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCGTGGCCCTGGcctg                                                                                                                                         >  4:138805/1‑146 (MQ=255)
          tttCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGT‑CTGGAGTACCACATCGTTGGGCTTGCCCAGCGCACCGGTGCTCAACGCCTCGTCCCCCAGGTTGCCCCAGGCCAGGGCCCCGCCCTGGCCC‑Ct                                                                                                                                       >  4:304220/1‑148 (MQ=255)
          tttCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑Ct                                                                                                                                       >  1:278699/1‑148 (MQ=255)
              aCGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATc                                                                                                                                   <  4:275228/148‑1 (MQ=255)
                   gACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGAc                                                                                                                             >  3:87735/1‑149 (MQ=255)
                      cACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCTGT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGTCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTgg                                                                                                                          <  1:147193/149‑1 (MQ=255)
                      cACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTgg                                                                                                                          <  5:189157/149‑1 (MQ=255)
                      cACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTg                                                                                                                           >  2:97881/1‑148 (MQ=255)
                          cTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACAAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCa                                                                                                                      <  1:97881/149‑1 (MQ=255)
                             ggTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACAAAACATCCACAAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCa                                                                                                                   >  1:306977/1‑149 (MQ=255)
                              gTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCACCAGGCCAGGGCCACGCCCAGGCCA‑CTGATCGACGACTGGATCAGCa                                                                                                                   >  7:18971/1‑148 (MQ=255)
                                 gacgacTCCAGCAGGCAGAACTTGGCGTCGGT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATAc                                                                                                                <  1:30917/148‑1 (MQ=255)
                                    gacTCCAGCAGGCAGAACTTGGCGTCGGT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACCTGATCGCCGACTGGATCAGCATACCGt                                                                                                             <  1:16773/149‑1 (MQ=255)
                                     acTCCAGCAGGCAGAACTTGGCGTCGGT‑CTGGTGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAg                                                                                                           <  2:326371/149‑1 (MQ=255)
                                      cTCCAGCAGGCAGAACTTGGCGTCGGT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAg                                                                                                           >  8:118877/1‑148 (MQ=255)
                                         cagcagGCAGAACTTGGCGTCGGTACTGGATTACCACATCGTTGGGCTTGACCAGCGCACCGGTGATCAACCCATCGTCCCCCAGGTTGCCCCAGGCCAGGGACACCCCATGGCAA‑CTGATCGCCGACTGGATCACCAAACCCTAGCTg                                                                                                        >  7:230738/1‑149 (MQ=255)
                                         cagcagGCAGAACTTGGCGTCGGT‑CTGGAGTACCACTTCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGGATCGTCCTCCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATTGCGTAggt                                                                                                         <  8:219238/147‑3 (MQ=255)
                                         cagcagGCAGAACTTGGCGTCGGT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTg                                                                                                        <  8:119247/148‑1 (MQ=255)
                                         cagcagGCAGAACTTGGCGTCGGT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGt                                                                                                       <  7:115574/149‑1 (MQ=255)
                                         cagcagGCAGAACTTGGCGTCGGT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGt                                                                                                       >  6:274573/1‑149 (MQ=255)
                                          agcagGCAGAACTTGGCGTTGGT‑CTGGAGTACCACATCGTTGGGATTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGtt                                                                                                      <  8:230738/149‑1 (MQ=255)
                                               gcagAACTTGGCGTCGGT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGatat                                                                                                 >  1:969/1‑149 (MQ=255)
                                               gcagAACTTGGCGTCGGT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGa                                                                                                    <  8:274433/146‑1 (MQ=255)
                                               gcagAACTTGGCGTCGGT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGa                                                                                                    >  7:274433/1‑146 (MQ=255)
                                               gcagAACTTGGCGTCGGT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACCCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGAAGCTGTTGatat                                                                                                 >  2:219398/1‑149 (MQ=255)
                                                cagAACTTGGCGTCGGT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATAtt                                                                                                <  5:274573/149‑1 (MQ=255)
                                                 agAACTTGGCGTCGGT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATAtt                                                                                                >  3:206680/1‑148 (MQ=255)
                                                 agAACTTGGCGTCGGT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATAtt                                                                                                >  4:202957/1‑148 (MQ=255)
                                                 agAACTTGGCGTCGGT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGATGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGAAGCTGTTGATAtt                                                                                                >  2:295150/1‑148 (MQ=255)
                                                     cTTGGCGTCGGT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGAcgc                                                                                           <  4:176385/149‑1 (MQ=255)
                                                           gTCGGT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCt                                                                                     >  7:355851/1‑149 (MQ=255)
                                                              ggT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTg                                                                                   <  2:374737/148‑1 (MQ=255)
                                                              ggT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTg                                                                                   <  2:278699/148‑1 (MQ=255)
                                                                t‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTgggg                                                                                >  2:75274/1‑149 (MQ=255)
                                                                 tccgcaGTACCACATCGCTCCGCTGCACCACCGCACCGGTCCCCCCCGCATCGTCCACCAGGTTCCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTgggg                                                                                <  4:285600/144‑1 (MQ=255)
                                                                     gAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCa                                                                            >  2:6433/1‑149 (MQ=255)
                                                                       gTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATg                                                                          <  6:105519/149‑1 (MQ=255)
                                                                             caTCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCg                                                                    >  4:216289/1‑149 (MQ=255)
                                                                              aTCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCg                                                                    >  1:200922/1‑148 (MQ=255)
                                                                                 gTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATc                                                                >  7:258494/1‑149 (MQ=255)
                                                                                    gggCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATcc                                                               >  6:147051/1‑147 (MQ=255)
                                                                                    gggCGTGACCAGCGCCCCGGTTCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCTACTGGATCAGCATACCGTAGCTGTTGATATTGACTCGATGCTTGGGGGTCATCGGCTCGTATccc                                                              <  6:74055/148‑1 (MQ=255)
                                                                                     ggCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCa                                                             <  3:26311/148‑1 (MQ=255)
                                                                                     ggCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAg                                                            <  5:147051/149‑1 (MQ=255)
                                                                                       cTTGACCAGCGCACCGGTGCTCAACGAATCGGCCACCAGGTTGCCCCAGGCCATGGCCACGCCCTTGCCA‑CTGATCGCCGAGTGGATCAGCATACCGTTGCTGTTGATATTGACGCGATTCTTGGGGGTCATGGGCTCGTTTCCCAGgc                                                          <  8:18971/149‑1 (MQ=255)
                                                                                         tGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGgcgc                                                        >  4:365663/1‑149 (MQ=255)
                                                                                         tGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGgcgc                                                        >  2:172330/1‑149 (MQ=255)
                                                                                           aCCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCtt                                                      >  3:330163/1‑149 (MQ=255)
                                                                                            ccAGCGCAACGGTGCTCAACGCATCGTCCACCAGGTTGCCCCCGGCCAGGGCCACCCCCTGGCCA‑CTGATAGCCGAATGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGTGCTCGTATCACATgcga                                                        >  8:156224/1‑145 (MQ=255)
                                                                                             cAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTg                                                     >  3:90566/1‑148 (MQ=255)
                                                                                                cgcACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAAc                                                  >  6:105802/1‑148 (MQ=255)
                                                                                                      ggTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCgg                                           <  8:258494/149‑1 (MQ=255)
                                                                                                        tGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGc                                         <  1:75274/149‑1 (MQ=255)
                                                                                                        tGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGc                                         <  5:105802/149‑1 (MQ=255)
                                                                                                         gCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGc                                         >  5:10161/1‑148 (MQ=255)
                                                                                                           tCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCaa                                      >  6:242044/1‑149 (MQ=255)
                                                                                                             aaCGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATAGCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAAc                                     <  7:118877/148‑1 (MQ=255)
                                                                                                             aaCGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCaa                                      <  7:156224/147‑1 (MQ=255)
                                                                                                             aaCGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCaa                                      <  1:172330/147‑1 (MQ=255)
                                                                                                             aaCGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCaa                                      <  3:365663/147‑1 (MQ=255)
                                                                                                                    cGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGgcgc                                                        >  7:294878/1‑122 (MQ=255)
                                                                                                                    cGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGgcgc                                                        <  8:294878/122‑1 (MQ=255)
                                                                                                                     gTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGt                            >  7:211788/1‑149 (MQ=255)
                                                                                                                         accaGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCAGACCGTAGCTGTTGATATTGACGCGATGGTTGGGGGTCATGGGCTCGTATCCCAGCCGCTTGAACCATACGGGCCAACCTACCCAGCcgag                        >  2:304241/1‑147 (MQ=255)
                                                                                                                           caGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGTCGCGATGCTTGGGGGTCATGGGGTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCt                      <  2:306977/149‑1 (MQ=255)
                                                                                                                           caGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGCATCAGCAAACCGTAGCTCTTGATATTGACGCGATGCTAGGGGGTCATGGGCTCGTATCACAGGGGCTTGAACCATTCGGGCCAACCGACGCAGACGCGCt                      >  2:332923/1‑149 (MQ=255)
                                                                                                                             ggTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTgg                    <  4:330163/149‑1 (MQ=255)
                                                                                                                               ttGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGa                  <  2:200922/149‑1 (MQ=255)
                                                                                                                               ttGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGa                  <  4:197086/149‑1 (MQ=255)
                                                                                                                                 atcagtggccaggGCGTGGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGa                  <  4:311198/141‑1 (MQ=255)
                                                                                                                                 atcagtggccaggGCGTGGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGa                  >  3:311198/7‑147 (MQ=255)
                                                                                                                                   cccAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCt              >  7:276187/1‑149 (MQ=255)
                                                                                                                                   cccAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCt              >  1:47181/1‑149 (MQ=255)
                                                                                                                                   cccAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCt              <  3:304220/149‑1 (MQ=255)
                                                                                                                                    ccaggccaggGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCtt             >  6:287336/1‑149 (MQ=255)
                                                                                                                                    ccaggccaggGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCtt             <  3:216289/149‑1 (MQ=255)
                                                                                                                                        gccaggGCGTGGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTCAag         >  3:11475/1‑149 (MQ=255)
                                                                                                                                         ccaggGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTCAagc        <  1:295150/149‑1 (MQ=255)
                                                                                                                                           aggGCCATTCCCTGCACA‑CTGATCGCAGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGTCGCTTTAACCATTCGGGCCAACCTAACCAGTCGCGCTGGGAGTCTTAAaggct      >  8:328552/1‑146 (MQ=255)
                                                                                                                                             ggCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTCAagccaga    <  3:202957/149‑1 (MQ=255)
                                                                                                                                             ggCCACGCCCTGGCCA‑CAGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTCAagccaga    <  3:326451/149‑1 (MQ=255)
                                                                                                                                               ccACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTCAagccagagc  >  7:104911/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
AGCCGCACGCTTTCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGT‑CTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCA‑CTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTTCAAGCCAGAGC  >  NC_002947/6127019‑6127309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑