Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 448,977 G→A 5.8% V50I (GTC→ATC)  PP_0370 → acyl‑CoA dehydrogenase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947448,9770GA 5.8% 211.3 / 2.4 69V50I (GTC→ATC) PP_0370acyl‑CoA dehydrogenase family protein
Reads supporting (aligned to +/- strand):  ref base G (33/32);  new base A (2/2);  total (35/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

CTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGCGGACCCCAGCCGGTTTCATTGAAGCCTACAACACCTACGCCGAAGGCGGCTGGGTGGGCGTGGGCGGCGACCC  >  NC_002947/448834‑449125
                                                                                                                                               |                                                                                                                                                    
cTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCAcc                                                                                                                                                 >  5:130450/1‑149 (MQ=255)
    cTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCa                                                                                                                                             <  7:277187/149‑1 (MQ=255)
     tataAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAg                                                                                                                                            >  6:202124/1‑149 (MQ=255)
         aaGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGAc                                                                                                                                         >  8:211822/1‑148 (MQ=255)
           gcgcCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCAt                                                                                                                                      <  6:123948/149‑1 (MQ=255)
            cgccgcTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCAtt                                                                                                                                     <  4:304406/149‑1 (MQ=255)
                       gACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAg                                                                                                                          <  1:210216/149‑1 (MQ=255)
                         cATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAgcc                                                                                                                        <  2:193845/149‑1 (MQ=255)
                          aTGCGCTTCGTACTGAATGAAGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACAGCCAGGACCATTGCGCCGCTCAgccg                                                                                                                       >  3:229941/1‑149 (MQ=255)
                            gcgcTTCGTACTGAATGAAGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAgccgc                                                                                                                      >  8:328060/1‑148 (MQ=255)
                            gcgcTTCGTACTGAATGAAGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAgccgc                                                                                                                      <  7:328060/148‑1 (MQ=255)
                                   gTACTGAATGTAGTCTTCTACGTGGAGGAGCAGTGGACTCAGTTGCCCGGGCGGGCGGAGGTTCTCGAGGCAGACACGTCCATGGCCGTTCTGGAAGATGCCGGCATGGTGAGCGCCAGGACCTTTGCGCCGCTCAGCCGCGCCGCcga                                                                                                              <  4:103208/149‑1 (MQ=255)
                                         aatgaaGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGaa                                                                                                          >  1:245322/1‑147 (MQ=255)
                                           tgaaGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGACGCTCAGCCGCGCAGCCGACGAAGAgg                                                                                                      >  7:94559/1‑149 (MQ=255)
                                             aaGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTgcg                                                                                                                                  <  3:3329/119‑1 (MQ=255)
                                             aaGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCAAGGACCATTgcg                                                                                                                                  >  4:3329/1‑119 (MQ=255)
                                              aGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCt                                                                                                   <  5:154563/149‑1 (MQ=255)
                                               gTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTg                                                                                                  >  8:142267/1‑149 (MQ=255)
                                                  ttCAACGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCa                                                                                               >  6:284895/1‑149 (MQ=255)
                                                    cAACGTGGCCGAGCAGTGGACGAAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACt                                                                                             >  2:43340/1‑149 (MQ=255)
                                                      aCGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTgg                                                                                           <  4:229941/149‑1 (MQ=255)
                                                        gTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGa                                                                                         >  8:135784/1‑149 (MQ=255)
                                                          ggCCGAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGAc                                                                                        >  4:174259/1‑148 (MQ=255)
                                                           gCCGAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACaa                                                                                      <  5:284895/149‑1 (MQ=255)
                                                             cGAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACg                                                                                    >  6:288987/1‑149 (MQ=255)
                                                              gAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGATCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTg                                                                                            <  5:290639/140‑1 (MQ=255)
                                                              gAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGATCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTg                                                                                            >  4:86488/1‑140 (MQ=255)
                                                              gAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGATCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTg                                                                                            <  3:86488/140‑1 (MQ=255)
                                                              gAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGATCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTg                                                                                            >  6:290639/1‑140 (MQ=255)
                                                                      gACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACgg                                                                                   <  4:9112/141‑1 (MQ=255)
                                                                      gACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACgg                                                                                   >  3:9112/1‑141 (MQ=255)
                                                                      gACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGc                                                                           <  8:94559/149‑1 (MQ=255)
                                                                      gACGAAGTTGCCCGGGATGGCCGAGGTTGTGGATGCCGCCACGGCCATGGCGGTGCGGGAACAAGCCGGCAAGGTAACCGCCAGGACCATTGCGCCGCGCAGCCGCGCCGCCGACGAAGAGGCGGCCCAGGGGGACAACGGCGCGGtcc                                                                           >  4:342612/1‑147 (MQ=255)
                                                                       aCGCAGTTGCCCGGGCTGGCCGTGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGCg                                                                          <  3:342612/149‑1 (MQ=255)
                                                                         gCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGCGGa                                                                        <  4:307479/149‑1 (MQ=255)
                                                                            gTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGCGGAccc                                                                     <  2:245322/149‑1 (MQ=255)
                                                                            gTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGCGGAccc                                                                     >  2:317872/1‑149 (MQ=255)
                                                                               gCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGt                                                                             <  1:237536/138‑1 (MQ=255)
                                                                               gCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGt                                                                             >  2:237536/1‑138 (MQ=255)
                                                                                         gCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGCGGACCCCAGCCGGTTTCa                                                         <  3:164112/148‑1 (MQ=255)
                                                                                          ccGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGCGGACCCCAGCCGGTTTCAtt                                                       >  2:287176/1‑149 (MQ=255)
                                                                                          ccGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGCGGACCCCAGCCGGTTTCAtt                                                       <  4:338401/149‑1 (MQ=255)
                                                                                          ccGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGCGGACCCCAGCCGGTTTCAtt                                                       <  6:143003/149‑1 (MQ=255)
                                                                                          ccGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGCGGACCCCAGCCGGTTTCAtt                                                       <  2:209608/149‑1 (MQ=255)
                                                                                          ccGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGCGGACCCCAGCCGGTTTCAtt                                                       <  6:130450/149‑1 (MQ=255)
                                                                                            gAGGGTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGCGGACCCCAGCCGGTTTCATTGa                                                     <  5:270242/149‑1 (MQ=255)
                                                                                                  gTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGCGGACCCCAGCCGGTGTCATTGAAGCCTa                                               >  8:130759/1‑149 (MQ=255)
                                                                                                  gTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGACAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGCGGACCCCAGCCGGTTTCATTGAAGCCTa                                               >  4:202946/1‑149 (MQ=255)
                                                                                                    cGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCt                                                                                                   >  6:45352/1‑95 (MQ=255)
                                                                                                     gATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGCGGACCCCAGCCGGTTTCATTGAAGCCTacaa                                            <  7:142267/149‑1 (MQ=255)
                                                                                                          cagcacGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGCGGACCCCAGCCGGTTTCATTGAAGCCTACAACAcc                                        >  3:36988/4‑148 (MQ=255)
                                                                                                           gACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGCGGACCCCAGCCGGTTTCATTGAAGCCTACAACACCTa                                      >  7:223669/1‑149 (MQ=255)
                                                                                                           gACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGCGGACCCCAGCCGGTTTCATTGAAGCCTACAACACCTa                                      <  7:135784/149‑1 (MQ=255)
                                                                                                              acGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGCGGACCCCAGCCGGTTTCATTGAAGCCTACAACACCTACGc                                   >  7:182487/1‑149 (MQ=255)
                                                                                                                ggCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGCGGACCCCAGCCGGTTTCATTGAAGCCTACAACACCTACGcc                                  >  1:138954/1‑148 (MQ=255)
                                                                                                                 gCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGCGGGCTGCCACTGGGACAACGGCGCGGTGCGGACCCCAGCCGGTTTCATTGAAGCCTACAACACCTACGCCg                                 >  4:189277/1‑148 (MQ=255)
                                                                                                                  ccATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGCGCTGCCACTGGGACAACGGCGCGGTGCGGACCCCAGCCGGTTTCATAGAAGCCTACAACACCTACGCCg                                 >  1:345555/1‑147 (MQ=255)
                                                                                                                     tGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGCGGACCCCAGCCGGTTTCATTGAAGCCTACAACACCTACGCCGAAggc                            <  3:174259/149‑1 (MQ=255)
                                                                                                                         cGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGCGGACCCCAGCCGGTTTCATTGAAGCCTACAACACCTACGCCGAAGGCGGCt                        <  2:270263/149‑1 (MQ=255)
                                                                                                                               ggAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGCGGACCCCAGCCGGTTTCATTGAAGCCTACAACACCTACGCCGAAGGCGGCtgggtgg                  >  2:12787/1‑149 (MQ=255)
                                                                                                                               ggAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGCGGACCCCAGCCGGTTTCATTGAAGCCTACAACACCTACGCCGAAGGCGGCtgggtgg                  >  3:194571/1‑149 (MQ=255)
                                                                                                                                gaagaaGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGCGGACCCCAGCCGGTTTCATTGAAGCCTACAACACCTACGCCGAAGGCGGCtgggtggg                 >  3:190186/1‑149 (MQ=255)
                                                                                                                                 aagaagCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGCGGACCCCAGCCGGTTTCATTGAAGCCTACAACACCTACGCCGAAGGCGGCTGGgtgggc                >  5:295757/1‑149 (MQ=255)
                                                                                                                                  agaagCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGCGGACCCCAGCCGGTTTCATTGAAGCCTACAACACCTACGCCGAAGGCGGCTGGgtgggcg               >  2:299186/1‑149 (MQ=255)
                                                                                                                                     agCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGCGGACCCCAGCCGGTTTCATTGAAGCCTACAACACCTACGCCGAAGGCGGCTGGgtgggcgtgg            <  2:364703/149‑1 (MQ=255)
                                                                                                                                      gCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGCGGACCCCAGCCGGTTTCATTGAAGCCTACAACACCTACGCCGAAGGCGGCTGGgtgggcgtggg           <  8:223669/149‑1 (MQ=255)
                                                                                                                                        cGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGCGGACCCCAGCCGGTTTCATTGAAGCCTACAACACCTACGCCGAAGGCGGCTGGGtgggcgtgggcg         <  1:12787/149‑1 (MQ=255)
                                                                                                                                         ggCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGCGGACCCCAGCCGGTTTCATTGAAGCCTACAACACCTACGCCGAAGGCGGCTGGGTGGGCGTGggcgg        <  1:287176/149‑1 (MQ=255)
                                                                                                                                         ggCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGCGGACCCCAGCCGGTTTCATTGAAGCCTACAACACCTACGCCGAAGGCGGCTGGGTGGGCGTGggcgg        <  4:36988/149‑1 (MQ=255)
                                                                                                                                               gTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGCGGACCCCAGCCGGTTTCATTGAAGCCTACAACACCTACGCCGAAGGCGGCTGGGTGGGCGTGGGCGGCGAccc  >  8:323035/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
CTGACTATAAAGCGCCGCTGCGCGACATGCGCTTCGTACTGAATGAAGTCTTCAACGTGGCCGAGCAGTGGACGCAGTTGCCCGGGCTGGCCGAGGTTGTCGATGCCGACACGGCCATGGCCGTGCTGGAAGAAGCCGGCAAGGTCACCGCCAGGACCATTGCGCCGCTCAGCCGCGCCGCCGACGAAGAGGGCTGCCACTGGGACAACGGCGCGGTGCGGACCCCAGCCGGTTTCATTGAAGCCTACAACACCTACGCCGAAGGCGGCTGGGTGGGCGTGGGCGGCGACCC  >  NC_002947/448834‑449125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑