Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,147,577	A→T	10.3%	intergenic (‑227/+39)	PP_5392 ← / ← PP_5393	WD40/YVTN repeat‑containing protein/metal‑binding chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,147,577	0	A	T	10.3%	157.1 / 8.6	58	intergenic (‑227/+39)	PP_5392/PP_5393	WD40/YVTN repeat‑containing protein/metal‑binding chaperone
Reads supporting (aligned to +/- strand):  ref base A (30/22);  new base T (4/2);  total (34/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01

GAAGCATTTTCTTGATCAGCGTCAACGTCCACGGCTTGACTCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGACACTCACTGTACCGGCAGCACGGTCAACGATCA  >  NC_002947/6147430‑6147725                                                                                                                                                     |                                                                                                                                                     gAAGCATTTTCTTGATCAGCGTCAACCTCCACGGCTTGACTCGGTGCGGAGTGAGCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGAAAc                                                                                                                                                     <  2:344031/149‑1 (MQ=255)   aGCATTTTCTTGATCAGCGTCAACGTCCACGGCTTGACTCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACTTCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACAt                                                                                                                                                   <  1:60701/149‑1 (MQ=255)      aTTTTCTTGATCAGCGTCAACGTCCACGGCTTGACTCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCACGGCCGAATATGAAGAAACCGCGCCATGAAACATgg                                                                                                                                                 <  1:55426/149‑1 (MQ=255)       ttttCTTGATCAGCGTCAACGTCCACGGCTTGACTCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCa                                                                                                                                               >  4:307725/1‑149 (MQ=255)        tttcgggATCAGCGTCAACGTCCACGGCTTGACTCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCAc                                                                                                                                              >  7:23465/7‑149 (MQ=255)                  aGCGTCAACGTCCACGGCTTGACTCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCAcggc                                                                                                                                           <  1:340206/142‑1 (MQ=255)                  aGCGTCAACGTCCACGGCTTGACTCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCAcggc                                                                                                                                           >  2:340206/1‑142 (MQ=255)                   gCGTCAACGTCCACGGCTTGACTCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGaaa                                                                                                                                                      >  7:10731/1‑130 (MQ=255)                   gCGTCAACGTCCACGGCTTGACTCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGaaa                                                                                                                                                      <  8:10731/130‑1 (MQ=255)                         aCGTCCACGGCTTGACTCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCA‑GGCCGCATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCt                                                                                                                               >  6:117052/1‑147 (MQ=255)                         aCGTCCACGGCTTGACTCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGa                                                                                                                             <  5:247246/149‑1 (MQ=255)                            tCCACGGCTTGACTCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCg                                                                                                                          >  4:65145/1‑149 (MQ=255)                             ccACGGCTTGACTCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACCGCGGCTTGACATCTGAGAgg                                                                                                                         >  6:83663/1‑149 (MQ=255)                               aCGGCTTGACTCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGTTTCATGGCGcggt                                                                                                                                           >  5:313129/1‑128 (MQ=255)                               aCGGCTTGACTCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGTTTCATGGCGcggt                                                                                                                                           <  6:313129/129‑2 (MQ=255)                                      gACTCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACgag                                                                                                                  <  8:209652/147‑1 (MQ=255)                                       aCTCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACt                                                                                                               >  5:100063/1‑149 (MQ=255)                                         tCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGTGCCATGTTTCATGGCGcggtttc                                                                                                                                        <  6:116167/122‑5 (MQ=255)                                         tCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGTGCCATGTTTCATGGCGcggtttc                                                                                                                                        >  5:116167/1‑118 (MQ=255)                                           ggTGCGTAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAg                                                                                                            <  8:23465/148‑1 (MQ=255)                                                      aTCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCA‑TGCCGAATATGAAGACACCGCGCCATGAAACATGGCACGGCGGCTTGCCATATGAGCGGCTACGAGACTTAGCGAATGACttgg                                                                                                >  6:303479/1‑147 (MQ=255)                                                         gAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTttggtt                                                                                             <  3:112761/149‑1 (MQ=255)                                                           aGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTttggttgg                                                                                           <  4:259812/149‑1 (MQ=255)                                                           aGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTttggttgg                                                                                           >  4:302399/1‑149 (MQ=255)                                                               cGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTaa                                                                                       <  3:65145/149‑1 (MQ=255)                                                               cGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTaa                                                                                       >  5:50934/1‑149 (MQ=255)                                                                 tCCACTTTTTGGGGAGTATCGCTGCCTATCTCGACCCGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCAACAGAGCGGCAACGAGACTTATCGACTGACTTTGGTTGGCTAAc                                                                                      >  7:249773/1‑148 (MQ=255)                                                                     cTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAgc                                                                                 >  8:169345/1‑149 (MQ=255)                                                                          tGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCt                                                                             <  3:307725/148‑1 (MQ=255)                                                                           ggggAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTg                                                                            <  3:132451/148‑1 (MQ=255)                                                                           ggggAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTg                                                                            >  1:322951/1‑148 (MQ=255)                                                                               aGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGAc                                                                         >  8:247662/1‑147 (MQ=255)                                                                                     gCTGGCTATCTCGACCCGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCAc                                                                 <  3:302399/149‑1 (MQ=255)                                                                                         gCTATCTCGACCCGCATTCAGTCGAGATCT‑TGCCTAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGAc                                                             <  8:135816/149‑1 (MQ=255)                                                                                           tATCTCGACCCGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGAcgc                                                           >  1:201615/1‑149 (MQ=255)                                                                                               tcGACCCGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTc                                                       >  2:96696/1‑149 (MQ=255)                                                                                                    ccGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACttt                                                                                                 >  5:2423/1‑102 (MQ=255)                                                                                                    ccGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACttt                                                                                                 <  6:2423/102‑1 (MQ=255)                                                                                                          tcagtcGACATCA‑TGCCGAATATGAAGATACCGAGCGATGATACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATg                                             <  8:249773/148‑1 (MQ=255)                                                                                                            agtcGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGtt                                           <  2:210811/148‑1 (MQ=255)                                                                                                            agtcGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTg                                          >  8:229986/1‑149 (MQ=255)                                                                                                             gtcGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGc                                         >  4:257713/1‑149 (MQ=255)                                                                                                              tcGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCCGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCt                                        >  8:196733/1‑149 (MQ=255)                                                                                                                gACATCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCtt                                       <  7:338221/148‑1 (MQ=255)                                                                                                                 aCATCA‑GGCCGAATATGAAGAAACCGCGCCTTGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTg                                     >  1:34291/1‑149 (MQ=255)                                                                                                                   aTCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGa                                   <  7:229986/149‑1 (MQ=255)                                                                                                                   aTCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGa                                   <  7:247662/149‑1 (MQ=255)                                                                                                                     cA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGaca                                 >  8:222548/1‑149 (MQ=255)                                                                                                                         gCCGATTATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGAcactc                              <  4:317561/149‑1 (MQ=255)                                                                                                                         gCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGAcactc                              >  8:61851/1‑149 (MQ=255)                                                                                                                                  gaagaaACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGACACTCACTGTACCg                     >  1:19081/1‑149 (MQ=255)                                                                                                                                   aagaaACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGAcactcac                            >  2:285219/1‑141 (MQ=255)                                                                                                                                   aagaaACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGAcactcac                            <  1:285219/141‑1 (MQ=255)                                                                                                                                   aagaaACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGACACTCACTGTACCgg                    >  4:272773/1‑149 (MQ=255)                                                                                                                                     gaaACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGACACTCACTGTACCgg                    >  2:120614/1‑147 (MQ=255)                                                                                                                                      aaaCCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGACACTCACTGTACCGgcag                 >  2:103852/1‑149 (MQ=255)                                                                                                                                        gccGTGCCATGTTTCATGGCGCGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGACACTCACTGTACCGgcagca               >  7:342987/2‑149 (MQ=255)                                                                                                                                         ccGTGCCATGTTTCATGGCGCGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGACACTCACTGTACCGgc                   >  5:309095/1‑144 (MQ=255)                                                                                                                                                aTGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGCCGCGTTCCGCCTCGATGTTGCTTTGGACACTCACTGTACCGGCAGCACGGTCAAc       >  5:342482/1‑149 (MQ=255)                                                                                                                                                  gAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCACTGCACCGACGCGTTCCGCCTCGATGTTGCTATGGACACTACCTCAACCGGCAGCACGGTCAACGa     >  8:185980/1‑149 (MQ=255)                                                                                                                                                   aaaCATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGACACTCACTGTACCGGCAGCACGGTCAACGAt    <  2:201615/149‑1 (MQ=255)                                                                                                                                                    aaCATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGACACTCACTGTACCGGCAGCACGGTCAACGAt    >  5:6263/1‑148 (MQ=255)                                                                                                                                                     aCATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGACACTCACTGTACCGGCAGCACGGTCAACGATCa  >  3:341896/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                     GAAGCATTTTCTTGATCAGCGTCAACGTCCACGGCTTGACTCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCA‑GGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGACACTCACTGTACCGGCAGCACGGTCAACGATCA  >  NC_002947/6147430‑6147725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑