Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,147,584 A→G 11.3% intergenic (‑234/+32) PP_5392 ← / ← PP_5393 WD40/YVTN repeat‑containing protein/metal‑binding chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,147,5840AG11.3% 161.8 / 9.6 62intergenic (‑234/+32)PP_5392/PP_5393WD40/YVTN repeat‑containing protein/metal‑binding chaperone
Reads supporting (aligned to +/- strand):  ref base A (33/22);  new base G (4/3);  total (37/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01

TTTTCTTGATCAGCGTCAACGTCCACGGCTTGACTCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGACACTCACTGTACCGGCAGCACGGTCAACGATCACC  >  NC_002947/6147436‑6147727
                                                                                                                                                    |                                                                                                                                               
ttttCTTGATCAGCGTCAACGTCCACGGCTTGACTCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCa                                                                                                                                                 >  4:307725/1‑149 (MQ=255)
 tttcgggATCAGCGTCAACGTCCACGGCTTGACTCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCAc                                                                                                                                                >  7:23465/7‑149 (MQ=255)
           aGCGTCAACGTCCACGGCTTGACTCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCAcggc                                                                                                                                             <  1:340206/142‑1 (MQ=255)
           aGCGTCAACGTCCACGGCTTGACTCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCAcggc                                                                                                                                             >  2:340206/1‑142 (MQ=255)
                  aCGTCCACGGCTTGACTCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCAGGCCGCATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCt                                                                                                                                 >  6:117052/1‑147 (MQ=255)
                  aCGTCCACGGCTTGACTCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGa                                                                                                                               <  5:247246/149‑1 (MQ=255)
                     tCCACGGCTTGACTCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCg                                                                                                                            >  4:65145/1‑149 (MQ=255)
                      ccACGGCTTGACTCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACCGCGGCTTGACATCTGAGAgg                                                                                                                           >  6:83663/1‑149 (MQ=255)
                        aCGGCTTGACTCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCAGGCCGAATATGAAGAAACCGCGCCATGTTTCATGGCGcggt                                                                                                                                             >  5:313129/1‑128 (MQ=255)
                        aCGGCTTGACTCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCAGGCCGAATATGAAGAAACCGCGCCATGTTTCATGGCGcggt                                                                                                                                             <  6:313129/129‑2 (MQ=255)
                               gACTCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACgag                                                                                                                    <  8:209652/147‑1 (MQ=255)
                                aCTCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACt                                                                                                                 >  5:100063/1‑149 (MQ=255)
                                  tCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCAGGCCGAATATGAAGAAACCGTGCCATGTTTCATGGCGcggtttc                                                                                                                                          <  6:116167/122‑5 (MQ=255)
                                  tCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCAGGCCGAATATGAAGAAACCGTGCCATGTTTCATGGCGcggtttc                                                                                                                                          >  5:116167/1‑118 (MQ=255)
                                    ggTGCGTAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAg                                                                                                              <  8:23465/148‑1 (MQ=255)
                                               aTCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCATGCCGAATATGAAGACACCGCGCCATGAAACATGGCACGGCGGCTTGCCATATGAGCGGCTACGAGACTTAGCGAATGACttgg                                                                                                  >  6:303479/1‑147 (MQ=255)
                                                  gAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTttggtt                                                                                               <  3:112761/149‑1 (MQ=255)
                                                    aGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTttggttgg                                                                                             >  4:302399/1‑149 (MQ=255)
                                                    aGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTttggttgg                                                                                             <  4:259812/149‑1 (MQ=255)
                                                        cGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTaa                                                                                         <  3:65145/149‑1 (MQ=255)
                                                        cGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTaa                                                                                         >  5:50934/1‑149 (MQ=255)
                                                          tCCACTTTTTGGGGAGTATCGCTGCCTATCTCGACCCGGAGTCAGTCGACATCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCAACAGAGCGGCAACGAGACTTATCGACTGACTTTGGTTGGCTAAc                                                                                        >  7:249773/1‑148 (MQ=255)
                                                              cTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAgc                                                                                   >  8:169345/1‑149 (MQ=255)
                                                                   tGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCt                                                                               <  3:307725/148‑1 (MQ=255)
                                                                    ggggAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTg                                                                              >  1:322951/1‑148 (MQ=255)
                                                                    ggggAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTg                                                                              <  3:132451/148‑1 (MQ=255)
                                                                        aGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGAc                                                                           >  8:247662/1‑147 (MQ=255)
                                                                              gCTGGCTATCTCGACCCGGAGTCAGTCGACATCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCAc                                                                   <  3:302399/149‑1 (MQ=255)
                                                                                  gCTATCTCGACCCGCATTCAGTCGAGATCTTGCCTAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGAc                                                               <  8:135816/149‑1 (MQ=255)
                                                                                    tATCTCGACCCGGAGTCAGTCGACATCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGAcgc                                                             >  1:201615/1‑149 (MQ=255)
                                                                                        tcGACCCGGAGTCAGTCGACATCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTc                                                         >  2:96696/1‑149 (MQ=255)
                                                                                             ccGGAGTCAGTCGACATCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACttt                                                                                                   >  5:2423/1‑102 (MQ=255)
                                                                                             ccGGAGTCAGTCGACATCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACttt                                                                                                   <  6:2423/102‑1 (MQ=255)
                                                                                                   tcagtcGACATCATGCCGAATATGAAGATACCGAGCGATGATACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATg                                               <  8:249773/148‑1 (MQ=255)
                                                                                                     agtcGACATCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGtt                                             <  2:210811/148‑1 (MQ=255)
                                                                                                     agtcGACATCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTg                                            >  8:229986/1‑149 (MQ=255)
                                                                                                      gtcGACATCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGc                                           >  4:257713/1‑149 (MQ=255)
                                                                                                       tcGACATCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCCGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCt                                          >  8:196733/1‑149 (MQ=255)
                                                                                                         gACATCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCtt                                         <  7:338221/148‑1 (MQ=255)
                                                                                                          aCATCAGGCCGAATATGAAGAAACCGCGCCTTGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTg                                       >  1:34291/1‑149 (MQ=255)
                                                                                                            aTCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGa                                     <  7:247662/149‑1 (MQ=255)
                                                                                                            aTCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGa                                     <  7:229986/149‑1 (MQ=255)
                                                                                                              cAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGaca                                   >  8:222548/1‑149 (MQ=255)
                                                                                                                 gCCGATTATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGAcactc                                <  4:317561/149‑1 (MQ=255)
                                                                                                                 gCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGAcactc                                >  8:61851/1‑149 (MQ=255)
                                                                                                                          gaagaaACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGACACTCACTGTACCg                       >  1:19081/1‑149 (MQ=255)
                                                                                                                           aagaaACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGAcactcac                              <  1:285219/141‑1 (MQ=255)
                                                                                                                           aagaaACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGAcactcac                              >  2:285219/1‑141 (MQ=255)
                                                                                                                           aagaaACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGACACTCACTGTACCgg                      >  4:272773/1‑149 (MQ=255)
                                                                                                                             gaaACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGACACTCACTGTACCgg                      >  2:120614/1‑147 (MQ=255)
                                                                                                                              aaaCCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGACACTCACTGTACCGgcag                   >  2:103852/1‑149 (MQ=255)
                                                                                                                                gccGTGCCATGTTTCATGGCGCGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGACACTCACTGTACCGgcagca                 >  7:342987/2‑149 (MQ=255)
                                                                                                                                 ccGTGCCATGTTTCATGGCGCGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGACACTCACTGTACCGgc                     >  5:309095/1‑144 (MQ=255)
                                                                                                                                        aTGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGCCGCGTTCCGCCTCGATGTTGCTTTGGACACTCACTGTACCGGCAGCACGGTCAAc         >  5:342482/1‑149 (MQ=255)
                                                                                                                                          gAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCACTGCACCGACGCGTTCCGCCTCGATGTTGCTATGGACACTACCTCAACCGGCAGCACGGTCAACGa       >  8:185980/1‑149 (MQ=255)
                                                                                                                                           aaaCATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGACACTCACTGTACCGGCAGCACGGTCAACGAt      <  2:201615/149‑1 (MQ=255)
                                                                                                                                            aaCATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGACACTCACTGTACCGGCAGCACGGTCAACGAt      >  5:6263/1‑148 (MQ=255)
                                                                                                                                             tcATGGCGCGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGACACTCACTGTACCGGCAGCACGGTCAACGATc     <  8:342987/147‑1 (MQ=255)
                                                                                                                                             aCATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGACACTCACTGTACCGGCAGCACGGTCAACGATCa    >  3:341896/1‑149 (MQ=255)
                                                                                                                                              cATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGACACTCACTGTACCGGCAGCACGGTCaa          >  7:301065/1‑142 (MQ=255)
                                                                                                                                              cATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGACACTCACTGTACCGGCAGCACGGTCaa          <  8:301065/142‑1 (MQ=255)
                                                                                                                                               aTGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGACACTCACTGTACCGGCAGCACGGTCAACGATCAcc  >  4:328699/1‑149 (MQ=255)
                                                                                                                                               aTGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCCGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGACACTCACTGTACCGGCAGCACGGTCAACGATCAcc  <  7:196733/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
TTTTCTTGATCAGCGTCAACGTCCACGGCTTGACTCGGTGCGGAGTGATCGAAGGCCGTCCACTTTTTGGGGAGTATCGCTGGCTATCTCGACCCGGAGTCAGTCGACATCAGGCCGAATATGAAGAAACCGCGCCATGAAACATGGCACGGCGGCTTGCCATCTGAGCGGCAACGAGACTTAGCGACTGACTTTGGTTGGGTAACCAAGCGCCTGGACCAGTGCACCGACGCGTTCCGCCTCGATGTTGCTTTGGACACTCACTGTACCGGCAGCACGGTCAACGATCACC  >  NC_002947/6147436‑6147727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑