Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,159,570 T→C 7.4% T250T (ACA→ACG)  PP_5402 ← NAD‑dependent acetyl‑CoA synthetase deacetylase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,159,5700TC 7.4% 157.9 / 2.4 54T250T (ACA→ACG) PP_5402NAD‑dependent acetyl‑CoA synthetase deacetylase subunit
Reads supporting (aligned to +/- strand):  ref base T (22/28);  new base C (2/2);  total (24/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

CGCAACAGCAGTGAAAGGCCCGTATCTTCTGAGGTAGCGTGGCGTGAGATATGTGCAAAAAAATGTGCACATCATCAGCGGATTGGGTGGTGTGCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCTGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCAGGTCCTTCGAGCATTATCTCGTCCGCTCCGTCCATTCCCACGTCTTCAAGGTTCACATGGATCACCGTTGCCCCGACCGCCAGAGCCATATCTGGAATACCGGCTGCCGG  >  NC_002947/6159424‑6159716
                                                                                                                                                  |                                                                                                                                                   
cgcAACAGCAGTGAAAGGCCCGTATCTTCTGAGGTAGCGTGGCGTGAGATATGTGCAAAAAAATGTGCACATCATCAGCGGATTGGGTGGTGTGCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCtgt                                                                                                                                                   <  7:331522/149‑1 (MQ=255)
   aaCAGCAGTGAAAGGCCCGTATCTTCTGAGGTAGCGTGGCGTGAGATATGTGCAAAAAAATGTGCACATCATCAGCGGATTGGGTGGTGTGCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCTGTGGc                                                                                                                                                <  7:207366/149‑1 (MQ=255)
     cagcagTGAAAGGCCCGTATCTTCTGAGGTAGCGTGGCGTGAGATATGTGCAAAAAAATGTGCACATCATCAGCGGATTGGGTGGTGTGCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCTGTGGC‑tt                                                                                                                                             <  7:129365/149‑1 (MQ=255)
     cagcagTGAAAGGCCCGTATCTTCTGAGGTAGCGTGGCGTGAGATATGTGCAAAAAAATGTGCACATCATCAGCGGATTGGGTGGTGTGCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCTGTGGC‑tt                                                                                                                                             >  2:8254/1‑149 (MQ=255)
      agcagTGTAAGGCCCGTATCTTCTGAGGTAGCGTGGCGTGAGATATGTGCAAAAAAATGTGCACATCATCAGCGGATTGGGTTGTGTTCAAGCTTGGCTCGTTAAGTATGAACGCCAGGCGGAGACAGAGCAGCAACCCCTGTGGC‑TTg                                                                                                                                            <  5:247054/149‑1 (MQ=255)
      agcagTGAAAGGCCCGTATCTTCTGAGGTAGCGTGGCGTGAGATATGTGCAAAAAAATGTGCACATCATCAGCGGATTGGGTGGTGTGCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCTGTGGC‑tt                                                                                                                                             >  1:155946/1‑148 (MQ=255)
        cagTGAAAGGCCCGTATCTTCTGAGGTAGCGTGGCGTGAGATATGTGCAAAATAATGTGCACATCATCAGCGGATTGGGTGGTGTGCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCTGTGGC‑TTGaa                                                                                                                                          <  3:351463/149‑1 (MQ=255)
                 gCCCGTATCTTCTGAGGTAGCGTGGCGTGAGATATGTGCAAAAAAATGTGCACATCATCAGCGGATTGGGTGGTGTGCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCTGTGGC‑TTGAAGCAACGCgg                                                                                                                                 <  3:355541/149‑1 (MQ=255)
                 gCCCGTATCTTCTGAGCTAGCGTGGCGTGAGATATGTGCAAAAAAATGTGCACATCATCAGCGTATTGGGTGGTGTGCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCTGTGGC‑TTGAAGCAACGCgg                                                                                                                                 <  7:276755/149‑1 (MQ=255)
                  cccGTATCTTCTGAGGTAGCGTGGCGTGAGATATGTGCAAAAAAATGTGCACATCATCAGCGGATTGGGTGGTGTGCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCTGTGGC‑TTGAAGCAACGCggg                                                                                                                                <  1:376262/149‑1 (MQ=255)
                     gTATCTTCTGAGGTAGCGTGGCGTGAGATATGTGCAAAAAAATGTGCACATCGTCAGCGGATTGGGTGGTGTGCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCTGTGGC‑TTGAAGCAACGCGGGTaa                                                                                                                             >  1:284237/1‑149 (MQ=255)
                          ttctGAGGTAGCGTGGCGTGAGATATGTGCAAAAAAATGTGCACATCATCAGCGGATTGGGTGGTGTGCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCTGTGGC‑TTGaa                                                                                                                                          >  4:207226/1‑131 (MQ=255)
                          ttctGAGGTAGCGTGGCGTGAGATATGTGCAAAAAAATGTGCACATCATCAGCGGATTGGGTGGTGTGCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCTGTGGC‑TTGaa                                                                                                                                          <  3:207226/131‑1 (MQ=255)
                           tctGAGGTAGCGTGGCGTGAGATATGTGCAAAAAAATGTGCACATCATCAGCGGATTGGGTGGTGTGCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCTGTGGC‑TTGAAGCAACGCGGGTAATaccac                                                                                                                       >  1:243403/1‑149 (MQ=255)
                            ctGAGGTAGCGTGGCGTGAGATATGTGCAAAAAAATGTGCACATCATCAGCGGATTGGGTGGTGTGCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCTATGGC‑TTGAAGCAACGCGGGTAATaccac                                                                                                                       <  2:56158/148‑1 (MQ=255)
                               aGGTAGCGTGGCGTGAGATATGTGCAAAAAAATGTGCACATCATCAGCGGATTGGGTGGTGTGCAAGCTTGGCTCGTTAAGTAGGACCGCCAGGCGGAGACAGAGCAGCAACCCGTGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCt                                                                                                                   >  8:315445/1‑149 (MQ=255)
                               aGGTAGCGTGGCGTGAGATATGTGCAAAAAAATGTGCACATCATCAGCGGATTGGGTGGTGTGCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCTGTGGC‑TTGAAGCAACGCGGGTAATACCAccc                                                                                                                     <  1:160421/147‑1 (MQ=255)
                                  tAGCGTGGCGTGAGATATGTGCAAAAAAATGTGCACATCATCAGCGGATTGGGTGGTGTGCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCTGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTg                                                                                                                  >  7:139526/1‑147 (MQ=255)
                                  tAGCGTGGCGTGAGATATGTGCAAAAAAATGTGCACATCATCAGCGGATTGGGTGGTGTGCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCTGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCa                                                                                                                <  8:139526/149‑1 (MQ=255)
                                       tggcgtgAGATATGTGCAAAAAAATGTGCACATCATCAGCGGATTGGGTGGTGTGCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCTGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCAGGTcc                                                                                                           <  2:155946/149‑1 (MQ=255)
                                        ggcgtgAGATATGTGCAAAAAAATGTGCACATCATCAGCGGATTGGGTGGTGTGCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCCGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCAGGTCCt                                                                                                          >  1:66326/1‑149 (MQ=255)
                                          agtgAGATATGTGCAAAAAAATGTGCACATCATCAGCGGATTGGGTGGTGTGCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCTGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCAGGTCCTTc                                                                                                        <  8:162848/148‑1 (MQ=255)
                                                 tatGTGCAAAAAAATGTGCACATCATCAGCGGATTGGGTGGTGTGCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCTGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCAGGTCCTTCGAGCAtt                                                                                                 <  2:284237/149‑1 (MQ=255)
                                                    gtgCAAAAAAATGTGCACATCATCAGCGGATTGGGTGGTGTGCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCTGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCa                                                                                                                <  4:91831/131‑1 (MQ=255)
                                                    gtgCAAAAAAATGTGCACATCATCAGCGGATTGGGTGGTGTGCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCTGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCAGGTCCTTCGAGCATTAtc                                                                                              >  3:152075/1‑149 (MQ=255)
                                                       cAAAAAAATGTGCACATCATCAGCGGATTGGGTGGTGTGCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCTGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCAGGTCCTTCGAGCATTATCTCg                                                                                           <  4:152075/149‑1 (MQ=255)
                                                         ataaaaTGTGCACATCATCAGCGGATTGGCTGGTGTGCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCTGTGGC‑TTGAATCAACGCGGGTAATACCACCCCTGCAGGTCCTTCGAGCATTATCTCGTc                                                                                         <  4:97790/147‑1 (MQ=255)
                                                               tgtgCACATCATCAGCGGATTGGGTGGTGTGCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCACCCCCTGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCAGGTCCTTCGAGCATTATCTCGTCCGCTcc                                                                                   >  3:216504/1‑149 (MQ=255)
                                                                     catcatCAGCGGATTGGGTGGTGTGCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCACTGTGGC‑TTGAAGGAACGCGGGTAATACCACCCCTGCTGGTCCTTCGAGCATTATCTCGTCCGCTCCGTCCAt                                                                             <  5:184886/149‑1 (MQ=255)
                                                                           caGCGGATTGGGTGGTGTGCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCTGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCAGGTcc                                                                                                           >  6:11736/1‑113 (MQ=255)
                                                                           caGCGGATTGGGTGGTGTGCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCTGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCAGGTcc                                                                                                           <  5:11736/113‑1 (MQ=255)
                                                                            aGCGGATTGGGTGGTGTGCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCCGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCAGGTCCTTCGAGCATTATCTCGTCCGCTCCGTCCATTCCCAc                                                                       <  2:66326/148‑1 (MQ=255)
                                                                                       tggtgTGCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCCGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCAGGTCCTTCGAGCATTATCTCGTCCGCTCCGTCCAt                                                                             >  3:81308/1‑131 (MQ=255)
                                                                                       tggtgTGCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCCGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCAGGTCCTTCGAGCATTATCTCGTCCGCTCCGTCCAt                                                                             <  4:81308/131‑1 (MQ=255)
                                                                                            tgCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCTGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCAGGTCCTTCGAGCATTATCTCGTCCGCTCCGTCCATTCCCACGTCTTCAAGGTTcaca                                                       <  2:243403/148‑1 (MQ=255)
                                                                                              cAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCTGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCAGGTCCTTCGAGCATTATCTCGTCCGCTCCGTCCATTCCCACGTCTTCAAGGTTCACATg                                                     >  6:372665/1‑148 (MQ=255)
                                                                                                 gCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCTGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCAGGTCCTTCGAGCATTATCTCGTCCGCTCCGTCCATTCCCACGTCTTCAAGGTTCACATGGAt                                                  >  2:254074/1‑148 (MQ=255)
                                                                                                          gTTAAGTAGGAAAGCCAGGCGGAGACATAGCAGCAAACCCTGTGGCTTTTATGCTTCGCGGGTTTTTTTTTCCCTGCAGGTCCTTCGAGCATTATCTCGTCCGCTCCGTCCATTCCCACGTCTTCAAGGTTCACATGGATCACCGTTg                                          <  1:121857/148‑1 (MQ=255)
                                                                                                              aGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCTGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCAGGTCCTTCGAGCATTATCTCGTCCGCTCCGTCCATTCCCACGTCTTCAAGGTTCACATGGATCACCGTTGCCCCGa                                    <  4:215778/149‑1 (MQ=255)
                                                                                                                tAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCTGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCAGGTCCTTCGAGCATTATCTCGTCCGCTCCGTCCATTCCCACGTCTTCAAGGTTCACATGGATCACCGTTGCCCCGAcc                                  >  1:197782/1‑149 (MQ=255)
                                                                                                                        ctaGGCGGAGACAGAGCAGCAACCCCTGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCAGGTCCTTCGAGCATTATCTCGTCCGCTCCGTCCATTCCCACGTCTTCaa                                                               <  7:153883/110‑1 (MQ=255)
                                                                                                                        ctaGGCGGAGACAGAGCAGCAACCCCTGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCAGGTCCTTCGAGCATTATCTCGTCCGCTCCGTCCATTCCCACGTCTTCaa                                                               >  8:153883/3‑112 (MQ=255)
                                                                                                                            gCGGAGACAGAGCAGCAACCCCTGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCAGGTCCTTCGAGCATTATCTCGTCCGCTCCGTCCATTCCCACGTCTTCAAGGTTCACATGGATCACCGTTGCCCCGACCGCCAGAGCCata                      <  3:180486/149‑1 (MQ=255)
                                                                                                                               gagaCAGAGCAGCAACCCCTGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCAGGTCCTTCGAGCATTATCTCGTCCGCTCCGTCCATTCCCACGTCTTCAAGGTTCACATGGATCACCGTTGCCCCGACCGCCAGAGCCATATCt                   >  7:78133/1‑149 (MQ=255)
                                                                                                                                agaCAGAGCAGCAACCCCTGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCAGGTCCTTCGAGCATTATCTCGTCCGCTCCGTCCATTCCCACGTCTTCAAGGTTCACATGGATCACCGTTGCCCCGACCGCCAGAGCCATATCTg                  <  5:372665/149‑1 (MQ=255)
                                                                                                                                  aCAGAGCAGCAACCCCTGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCAGGTCCTTCGAGCATTATCTCGTCCGCTCCGTCCATTCCCACGTCTTCAAGGTTCACATGGATCACCGTTGCCCCGACCGCCAGAGCCATATCTgg                 >  2:164406/1‑148 (MQ=255)
                                                                                                                                    agagCAGCAACCCCTGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCAGGTCCTTCGAGCATTATCTCGTCCGCTCCGTCCATTCCCACGTCTTCAAGGTTCACATGGATCACCGTTGCCCCGACCGCCAGAGCCATATCTg                  <  3:253461/145‑1 (MQ=255)
                                                                                                                                    agagCAGCAACCCCTGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCAGGTCCTTCGAGCATTATCTCGTCCGCTCCGTCCATTCCCACGTCTTCAAGGTAGACATGGATCACCGTTGCCCCGCCCGCCAGAGCCATATCTg                  >  4:253461/1‑145 (MQ=255)
                                                                                                                                      agcagcAACCCCTGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCAGGTCCTTCGAGCATTATCTCGTCCGCTCCGTCCATTCCCACGTCTTCAAGGTTCACATGGATCACCGTTGCCCCGACCGCCAGAGCCATATCTGGAATAc            >  3:166533/1‑149 (MQ=255)
                                                                                                                                         agcaACCCCTGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCAGGTCCTTCGAGCATTATCTCGTCCGCTCCGTCCATTCCCACGTCTTCAAGGTTCACATGGATCACCGTTGCCCCGACCGCCAGAGCCATATCTGGAATACCgg         <  2:197782/149‑1 (MQ=255)
                                                                                                                                                 cTGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCAGGTCCTTCGAGCATTAtctc                                                                                            >  3:194667/1‑58 (MQ=255)
                                                                                                                                                 cTGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCAGGTCCTTCGAGCATTAtctc                                                                                            <  4:194667/58‑1 (MQ=255)
                                                                                                                                                 cTGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCAGGTCCTTCGAGCATTATCTCGTCCGCTCCGTCCATTCCCACGTCTTCAAGGTTCACATGGATCACCGTTGCCCCGACCGCCAGAGCCATATCTGGAATACCGGCTGCCgg  >  2:120526/1‑148 (MQ=255)
                                                                                                                                                 cTGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCAGGTCCTTCGAGCATTATCTCGTCCGCTCCGTCCATTCCCACGTCTTCAAGGTTCACATGGATCACCGTTGCCCCGACCGCCAGAGCCATATCTGGAATACCGGCTGCCgg  >  6:67684/1‑148 (MQ=255)
                                                                                                                                                 cTGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCAGGTCCTTCGAGCATTATCTCGTCCGCTCCGTCCATTCCCACGTCTTCAAGGTTCACATGGATCACCGTTGCCCCGACCGCCAGAGCCATATCTGGAATACCGGCTGCCgg  >  6:129944/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CGCAACAGCAGTGAAAGGCCCGTATCTTCTGAGGTAGCGTGGCGTGAGATATGTGCAAAAAAATGTGCACATCATCAGCGGATTGGGTGGTGTGCAAGCTTGGCTCGTTAAGTAGGAACGCCAGGCGGAGACAGAGCAGCAACCCCTGTGGC‑TTGAAGCAACGCGGGTAATACCACCCCTGCAGGTCCTTCGAGCATTATCTCGTCCGCTCCGTCCATTCCCACGTCTTCAAGGTTCACATGGATCACCGTTGCCCCGACCGCCAGAGCCATATCTGGAATACCGGCTGCCGG  >  NC_002947/6159424‑6159716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑