| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 459,899 | C→T | 6.4% | intergenic (‑45/+34) | pqqB ← / ← pqqA | coenzyme PQQ synthesis protein B/coenzyme PQQ synthesis protein A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 459,899 | 0 | C | T | 6.4% | 277.4 / 6.3 | 93 | intergenic (‑45/+34) | pqqB/pqqA | coenzyme PQQ synthesis protein B/coenzyme PQQ synthesis protein A |
| Reads supporting (aligned to +/- strand): ref base C (45/42); new base T (2/4); total (47/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01 | |||||||||||
GTGGCTTTCAGGGTGCCATCACGGTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGAGGGTTGGGTTGTTCAGCTAC > NC_002947/459751‑460045 | gTGGCTTTCAGGGTGCCATCACGGTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGccc < 7:82198/149‑1 (MQ=255) tGGCTTTCAGGGTGCCATCACGGTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGcccc < 4:180326/149‑1 (MQ=255) tttCAGGGTGCCATCACGGTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCgg > 6:264304/1‑147 (MQ=255) cAGGGTGCCATCTCGGTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAgg < 3:15268/148‑1 (MQ=255) gcgagCCATCACGGTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACAGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGAAGAAACCGGATCGAAAAAACGAAAACCCCCCGGCAGGCCg > 8:139710/5‑149 (MQ=255) gTGCCATCACGGTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAg > 2:250272/1‑149 (MQ=255) gCCATCACGGTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGc < 5:264304/149‑1 (MQ=255) aTCACGGTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCAGGCAGGCCGAGGCGt > 3:40627/1‑148 (MQ=255) aCGGTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACCAAAACGCCTCGGCCTGCCGGGGCGttttc < 7:300560/149‑5 (MQ=255) cGGTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAAc < 1:310777/149‑1 (MQ=255) ggTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCTCGGCCTGCCGGGGCGttttcgt < 2:260005/149‑7 (MQ=255) ggTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAAcc < 4:64763/149‑1 (MQ=255) cccTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGGACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAg > 1:331180/1‑149 (MQ=255) cTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGc > 5:250722/1‑128 (MQ=255) cTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGc < 6:250722/128‑1 (MQ=255) cTTGCAGTTGACGCAGTTGCAGTTCCACTGCGCGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGCATCGAAAAAACGAAAACGCCCCCGCAGGCCGACGCGTGAAAACCAAAGcc > 6:37124/1‑149 (MQ=255) gCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTgg > 6:40336/1‑149 (MQ=255) ctgTTGACGCAGTTGCAGGTCCACTGCGGGAACCCGGCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCTGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGGCGAGGCGTGAAACCCTAAGCCTgg < 2:278686/146‑1 (MQ=255) cGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGc < 3:137698/142‑1 (MQ=255) cGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGc > 4:137698/1‑142 (MQ=255) cagttgcagttCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGtt < 5:40336/149‑1 (MQ=255) cgttgcaggtCCTCTGCGGGAACCCGCCACCGTCGGCGTAGCCGAGAACCTGGAGTTACATGGCCACTCTCTATGCAGAAACCTGATCCAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTa < 8:121623/148‑1 (MQ=255) agttgcagttCCACTGCGGGGACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTa < 2:331180/149‑1 (MQ=255) agttgcagttCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGt > 1:14939/1‑147 (MQ=255) agttgcagttCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTa < 6:323356/149‑1 (MQ=255) agttgcagttCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTa < 7:248299/149‑1 (MQ=255) gttgcagttCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAg > 8:68430/1‑149 (MQ=255) ttgcagttCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGc < 1:250272/149‑1 (MQ=255) gttCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTa > 3:170125/1‑142 (MQ=255) gttCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGt < 6:52179/149‑1 (MQ=255) gttCCACTGCGGCAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTa < 4:170125/142‑1 (MQ=255) ttCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGCCAGGCCCAGGCGAGAAACCCTAAGCCTGGCTTAGAGGTTAGCGAAGTa > 1:225576/1‑149 (MQ=255) cACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACa < 7:68430/148‑1 (MQ=255) cACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACAt > 4:202070/1‑149 (MQ=255) aCTTCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCCGATCGAAAAAACGAAAACGCCCCGACAGGCCGAGGCGTGAAACCCTAAGCCTGGCTAACCGGTTAGCGAAGTACATg > 8:327483/1‑149 (MQ=255) cTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATgg > 8:96195/1‑149 (MQ=255) cGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGt < 3:128006/147‑1 (MQ=255) cGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGt < 1:29522/147‑1 (MQ=255) ggAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTACGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACt > 5:356236/1‑149 (MQ=255) ggAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACt > 8:123958/1‑149 (MQ=255) aaCCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTc < 2:12343/149‑1 (MQ=255) cccGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCg > 6:133854/1‑148 (MQ=255) ccGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCg > 6:211592/1‑147 (MQ=255) cGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCTCTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGaa > 3:261710/1‑130 (MQ=255) cGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTATCGGTTAGCGAAGTACATGGTGACTTCGAAg < 4:40627/149‑1 (MQ=255) cGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGaa < 4:261710/130‑1 (MQ=255) cGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACGCTCGCTGCAGAGGCCGGAGCGAAAAAACGAAAACGCCCCGGCAGGCCGCGGCGTGCACCCCTAAGCCTGGCGTAGCGGTTTGCGAAGTACATGGGGACTTCGacg > 5:374924/1‑147 (MQ=255) gCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAg > 8:36465/1‑149 (MQ=255) ccGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAACACCAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAgt < 2:361435/149‑1 (MQ=255) ccGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCGGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTa < 1:286331/108‑1 (MQ=255) ccGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCGGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTa > 2:286331/1‑108 (MQ=255) ccGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGAACGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAgt < 8:4729/149‑1 (MQ=255) gaACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTAt < 3:202070/149‑1 (MQ=255) gaACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGAACGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTCGCTTAGCGGTTAGCGAAGTACATCGTGACTTCGAAGCCGATACTCAGCTAAGTCTAt > 1:178806/1‑149 (MQ=255) ccTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAg < 2:364819/125‑1 (MQ=255) ccTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAg > 1:364819/1‑125 (MQ=255) gtGGATGAAAATGGCCACTCTGTATGCAGAAACGGGATCGAAAAAACCAAAACGCCCCGGCAGGCCGATGCGTGAAAGCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAg < 2:178806/148‑1 (MQ=255) cTGGATGGACCTGGCCACGCTCTAGCCAGCACCCGCATCGAAACAACGAAACCGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCGTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGCCAGTGTATGCAg < 6:374924/149‑1 (MQ=255) gATGTACATTGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCGGGCTGGCCGAGGCGTGAAACCCTAAGCCTgg < 3:303988/86‑1 (MQ=255) gATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGTCGTGAAACCCTAAGCCTgg > 4:303988/1‑86 (MQ=255) aCATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTc > 4:175241/1‑149 (MQ=255) cATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGAGTTCGAAGCCGATACGCAGGTCAGTCTATGCAGGTTTGGTc > 4:41634/1‑148 (MQ=255) cATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTc > 7:241433/1‑148 (MQ=255) aTGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAgg > 1:214971/1‑54 (MQ=255) aTGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAgg < 2:214971/54‑1 (MQ=255) aTGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGAGTATGCAGGTTTGGTcc > 7:42431/1‑148 (MQ=255) gCCTCTCTCTATGCAGAAACCGGATCGAAAAAACGAAAAGGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGGCTGGCTTAGCGTTTAGCCAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCcaca < 5:37124/148‑1 (MQ=255) gCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGAGTATGCAGGTTTGGTCCACAt < 8:42431/149‑1 (MQ=255) cACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATgg < 2:14939/149‑1 (MQ=255) cACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATg > 2:71707/1‑148 (MQ=255) tctATGGAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTa > 6:4546/1‑149 (MQ=255) ctATGCAGAAACCGGATCCAAAAAACGAAAACGCACCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAACCCGATACGCAGGTCAGTGTATGCAGGTTAGGTCCAGATGCAATTa > 8:223805/1‑148 (MQ=255) gCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTc > 8:305099/1‑147 (MQ=255) gCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTc < 7:305099/147‑1 (MQ=255) aGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTc > 5:125771/1‑149 (MQ=255) aGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTCCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTc > 1:13999/1‑149 (MQ=255) gAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTcc < 7:96195/149‑1 (MQ=255) aCCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGaa < 3:175241/149‑1 (MQ=255) ccGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAAt > 4:294494/1‑149 (MQ=255) ggATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGa < 6:356236/149‑1 (MQ=255) gAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGAgggtt < 5:211592/149‑1 (MQ=255) aaaaaaCGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGAgggttg > 3:212922/1‑149 (MQ=255) aaaaaaCGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGAgggttg > 5:198270/1‑149 (MQ=255) aaaacgaaaacgCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGAgggttggg > 7:21340/1‑149 (MQ=255) aacgaaaacgCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTTTGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGAgggttgggtt < 7:139710/149‑1 (MQ=255) gaaaacgCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGAGGGTTGGgttgtt > 2:51051/1‑149 (MQ=255) gaaaacgCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGAGGGTTGGgttgtt > 5:327482/1‑149 (MQ=255) taaacgCCCCGGCAGGCCGAGGCGTTAAACCCTAAGCCGGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAATCCTATACGCAGGTCAGTGTATGCAGGTTTGGTCCTCATGGAATTACTCCTTCCGAATGAGTGTTGGGTTGTTc < 6:337395/148‑1 (MQ=255) aaacgCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGTAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGAGGGTTGGGTTGTTc < 6:134815/148‑1 (MQ=255) acgCCCCTGCAGGCCGAGGCGTGAAACCCTAAGCCTGGGTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGAGGGTTGGGTTGTTCAGc < 7:327483/149‑1 (MQ=255) cgCCTCGGCCTGCCGGGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCTCATGGAATTACTCCTTCCGAATGAgggttgggt < 5:80203/140‑1 (MQ=255) cgCCTCGGCCTGCCGGGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGAgggttgggt < 5:85533/140‑1 (MQ=255) cgCCTCGGCCTGCCGGGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGAgggttgggt > 6:80203/1‑140 (MQ=255) cgCCTCGGCCTGCCGGGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGAgggttgggt > 6:85533/1‑140 (MQ=255) gCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGAGGGTTGGGTTGTTCAGc > 3:212650/1‑147 (MQ=255) cccGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGAGGGTTGGGTTGTTCAGctac > 8:184517/1‑148 (MQ=255) | GTGGCTTTCAGGGTGCCATCACGGTAGCCCTTGCAGTTGACGCAGTTGCAGTTCCACTGCGGGAACCCGCCACCGGCGGCGGAGCCGAGAACCTGGATGTACATGGCCACTCTCTATGCAGAAACCGGATCGAAAAAACGAAAACGCCCCGGCAGGCCGAGGCGTGAAACCCTAAGCCTGGCTTAGCGGTTAGCGAAGTACATGGTGACTTCGAAGCCGATACGCAGGTCAGTGTATGCAGGTTTGGTCCACATGGAATTACTCCTTCCGAATGAGGGTTGGGTTGTTCAGCTAC > NC_002947/459751‑460045 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |