Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	467,098	A→G	7.7%	intergenic (‑31/+22)	PP_5445 ← / ← PP_t71	hypothetical protein/tRNA‑Met

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	467,098	0	A	G	7.7%	184.8 / 4.6	65	intergenic (‑31/+22)	PP_5445/PP_t71	hypothetical protein/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base A (32/28);  new base G (3/2);  total (35/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

GCTGGGTGACCGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCACCTGTGCCATCTGAA  >  NC_002947/466956‑467246                                                                                                                                               |                                                                                                                                                       gcTGGGTGACCGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATcgcg                                                                                                                                                  <  6:197935/149‑1 (MQ=255)  cTGGGTGACCGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCTCAATcgc                                                                                                                                                   >  4:222774/1‑147 (MQ=255)       tGACCGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCtt                                                                                                                                            >  1:140872/1‑149 (MQ=255)           cGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTc                                                                                                                                        >  5:79017/1‑149 (MQ=255)           cGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTc                                                                                                                                        <  2:61123/149‑1 (MQ=255)                  ttGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCttt                                                                                                                                           >  4:235158/1‑139 (MQ=255)                  ttGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCttt                                                                                                                                           <  3:235158/139‑1 (MQ=255)                     gtggGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCtggt                                                                                                                               <  7:193312/148‑1 (MQ=255)                          gCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGcc                                                                                                                          >  8:83034/1‑148 (MQ=255)                              aTTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCacac                                                                                                                     <  4:168710/149‑1 (MQ=255)                              aTTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCacac                                                                                                                     <  7:193062/149‑1 (MQ=255)                                ttCGCTGAGTTAGCGTTTTAGCGCTACTTCCAAGGTCATGCGGTTGGCGCCGCTGCGGTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAATAAGCCGCGCGATTGCGCGGCtttttg                                                                                                                                        <  4:59672/128‑4 (MQ=255)                                ttCGCTGAGATACAGTTTTAGCGCTACTTCCAAGCTCAAGCGCTCGGCGCCGCTGCCCTGGATGTACGAGCCCCTGACCATTAAATTCTTGCGGGCATGAAAAACACGCCCGATTGCGCGGCtatatg                                                                                                                                        >  3:59672/1‑123 (MQ=255)                                   gCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑c                                                                                                               <  2:177507/148‑1 (MQ=255)                                   gCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑Ct                                                                                                              >  2:111783/1‑149 (MQ=255)                                    cTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTc                                                                                                             <  2:140872/149‑1 (MQ=255)                                     tGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTc                                                                                                             >  7:217164/1‑148 (MQ=255)                                     tGACATACCGTTTTAGCGCTACTTCCACGGGAATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGTCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCg                                                                                                            <  8:152796/149‑1 (MQ=255)                                       agaTACCGTTCTAGCGCTACTTCCACGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCg                                                                                                            <  4:349097/147‑1 (MQ=255)                                            ccGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGt                                                                                                      <  8:211192/148‑1 (MQ=255)                                                    gcgcTACTTCCCAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCaaa                                                                                              <  5:8507/148‑1 (MQ=255)                                                    gcgcTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGGGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGAc                                                                                                  >  7:198344/1‑144 (MQ=255)                                                    gcgcTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGAc                                                                                                  <  8:198344/144‑1 (MQ=255)                                                           ttCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTAt                                                                                       >  5:154099/1‑148 (MQ=255)                                                            tCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTAt                                                                                       >  8:326870/1‑147 (MQ=255)                                                              cAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTATGAGt                                                                                   >  3:180685/1‑149 (MQ=255)                                                              cAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCACGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTATGAGt                                                                                   >  5:190119/1‑149 (MQ=255)                                                                   tCATGTGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTATGAGTCctct                                                                              >  5:254210/1‑149 (MQ=255)                                                                     aTGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTATGAGTCCTCTg                                                                             <  3:222774/148‑1 (MQ=255)                                                                       gcgcTCGGCGCCGCTGCGCTGGATGTACGAGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGAGCGGCTTTGTCGAATCTGCTGGGCCCACACGGA‑‑CTAGAACCGTGGACCATAGGATTATGCGTACTCTGgtc                                                                          >  8:336195/1‑149 (MQ=255)                                                                        cgcTCGGCGCCGCTGCGCTTGATGTACGCGCCCCTGACCATTAATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGctct                                                                         <  8:310597/149‑1 (MQ=255)                                                                         gcTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGctct                                                                         >  7:89892/1‑148 (MQ=255)                                                                          cTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTaa                                                                       <  8:89892/149‑1 (MQ=255)                                                                          cTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTa                                                                        >  4:147281/1‑148 (MQ=255)                                                                           tCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCGATTGCGCGGCttttt                                                                                                                                         <  8:320534/84‑3 (MQ=255)                                                                           tCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCGATTGCGCGGCttttt                                                                                                                                         >  7:320534/1‑82 (MQ=255)                                                                                  cgctgcgctgGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGa                                                               <  7:326870/149‑1 (MQ=255)                                                                                   gctgcgctgGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAg                                                              <  7:83034/149‑1 (MQ=255)                                                                                        gctgGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCta                                                           >  5:190312/1‑147 (MQ=255)                                                                                          tgGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAGAGGATTATGAGTCCTCTGCTCTAAc                                                                      >  6:136834/1‑134 (MQ=255)                                                                                          tgGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAGAGGATTATGAGTCCTCTGCTCTAAc                                                                      <  5:136834/134‑1 (MQ=255)                                                                                          tgGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAgg                                                       <  1:158812/149‑1 (MQ=255)                                                                                              tGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTa                                                                                        <  8:289557/112‑1 (MQ=255)                                                                                              tGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTa                                                                                        >  7:289557/1‑112 (MQ=255)                                                                                                  cgcgCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGt                                               <  6:190312/149‑1 (MQ=255)                                                                                                   gcgcCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTCGTGGGCCCACACGGA‑‑CTCGAACCGTGGCCCATAGGCTTATGCGTCCTCTGCTCTTACCCACTGAGCTATAGGCCGTCAGt                                               >  8:343007/1‑148 (MQ=255)                                                                                                    cgcCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGACTCTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGt                                               <  1:111783/149‑1 (MQ=255)                                                                                                       cccTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTg                                          >  5:353292/1‑149 (MQ=255)                                                                                                        ccTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTgg                                         >  4:371396/1‑149 (MQ=255)                                                                                                             ccATTTATTTCTTGCGGGCATGAAAAAGCCGAGCAATCGCGCGGCTTTGTCGTTTCTGGTGGGCCCAAACGGA‑‑CTCGAACCGTGGACCAAAGGATTATTAGTCATATGATCTAAACAAATGAGCTATAGGCCATAAGTAAGTGGGCgca                                    >  5:89831/1‑147 (MQ=255)                                                                                                              cATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGAt                                   <  6:154099/149‑1 (MQ=255)                                                                                                               atttatatCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGACCAAACGGA‑‑ATCGAACCGTGGACCAAAGGATTATGAGTCCTCTCGACTACACAACTGAGCTATACGCCCACATGAAGTGGGCGGAt                                   >  8:304881/1‑148 (MQ=255)                                                                                                                   atttCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATaa                              >  1:251614/1‑149 (MQ=255)                                                                                                                    tttCTTGCGGGCATGACAAAGCCGCGCAATCGCGCGGGTTTGTCGTATCTGGGGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATaa                              <  6:295230/148‑1 (MQ=255)                                                                                                                     ttCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAAc                             >  7:345376/1‑148 (MQ=255)                                                                                                                     ttCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACAAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAAc                             >  1:149085/1‑148 (MQ=255)                                                                                                                      tCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATaa                              >  3:330529/1‑146 (MQ=255)                                                                                                                      tCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATaa                              <  4:330529/146‑1 (MQ=255)                                                                                                                        ttGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAgg                         >  7:295753/1‑149 (MQ=255)                                                                                                                            gggCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTATGAGTCGTCTGCTCTAAGCAACTGAGCTAAAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGttt                     >  7:71796/1‑149 (MQ=255)                                                                                                                                  gAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCAcc               >  8:222029/1‑149 (MQ=255)                                                                                                                                    aaaaGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCCTGGACCAAAGGATTATGAGTCCGCGGCTCTAACCACCTGAGCTATAGGCCCTGAGTAAGTGGGCGGATTATAACGAGGGTTTCGCACCtg             >  4:269672/1‑149 (MQ=255)                                                                                                                                     aaaGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCACCtgt            <  6:79017/149‑1 (MQ=255)                                                                                                                                        gCCGCGCGATTGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCACCTGTGc          >  2:67222/1‑148 (MQ=255)                                                                                                                                            cgcAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCACCTGTGCCATCt     <  3:147281/149‑1 (MQ=255)                                                                                                                                               aaTCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCACCTGTGCCATCTGaa  <  8:217164/149‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                       GCTGGGTGACCGGCTTCTTGGTGGGGCTGATTTCGCTGAGATACCGTTTTAGCGCTACTTCCAAGGTCATGCGCTCGGCGCCGCTGCGCTGGATGTACGCGCCCCTGACCATTTATTTCTTGCGGGCATGAAAAAGCCGCGCAATCGCGCGGCTTTGTCGTATCTGGTGGGCCCACACGGA‑‑CTCGAACCGTGGACCAAAGGATTATGAGTCCTCTGCTCTAACCAACTGAGCTATAGGCCCTCAGTAAGTGGGCGGATTATAACGAGGGTTTCGCACCTGTGCCATCTGAA  >  NC_002947/466956‑467246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑