Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	474,811	G→C	5.7%	F58L (TTC→TTG) ‡	dnaG ←	DNA primase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	474,811	0	G	C	5.7%	290.4 / 6.1	88	F58L (TTC→TTG) ‡	dnaG	DNA primase
Reads supporting (aligned to +/- strand):  ref base G (45/38);  new base C (2/3);  total (47/41)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.46e-01

CTTCTGGTCGCGGCGGCCTTGCTCGCGGGGCACTTCCATGCCGGCGGCGCGGGCCAGTTCCTCGACGGCCTGGGGGAAGTCCAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGCTGGACGCGCGAACTCACCACGTCGACGATGTCGAGGCGGTTGATCAGGTCGTCAATG  >  NC_002947/474664‑474958                                                                                                                                                     |                                                                                                                                                    cTTCTGGTCGCGGCGGCCTTGCTCGCGGGGCACTTCCATGCCGGCGGCGCGGGCCAGTTCCTCGACGGCCTGGGGGAAGTCCAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGtagtag                                                                                                                                                     >  2:80230/1‑148 (MQ=255)  ttCTGGTCGCGGCGGCCTTGCTCGCGGGGCACTTCCATGCCGGCGGCGCGGGCCAGTTCCTCGACGGCCTGGGGGAAGTCCAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGaa                                                                                                                                                   >  6:281857/1‑149 (MQ=255)       gTCGCGGCGGCCTTGCTCGCGGGGCACTTCCATGCCGGCGGCGCGGGCCAGTTCCTCGACGGCCTGGGGGAAGTCCAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGc                                                                                                                                               <  7:318283/148‑1 (MQ=255)         cgcgGCGGCCTTGCTCGCGGGGCACTTCCATGCCGGCGGCGCGGGCCAGTTCCTCGACGGCCTGGGGGAAGTCCAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTTCTACTACTGCttc                                                                                                                                            <  3:227802/149‑2 (MQ=255)          gcggcgGCCTTGCTCGCGGGGCACTTCCATGCCGGCGGCGCGGGCCAGTTCCTCGACGGCCTGGGGGAAGTCCAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTTCTACTACTGCttc                                                                                                                                            <  3:260386/148‑2 (MQ=255)           cggcggCCTTGCTCGCGGGGCACTTCCATGCCGGCGGCGCGGGCCAGTTCCTCGACGGCCTGGGGGAAGTCCAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTTCTACTACTGCttcgg                                                                                                                                          <  6:305621/149‑4 (MQ=255)              cggcCTTGCTCGCGGGGCACTTCCATGCCGGCGGCGCGGGCCAGTTCCTCGACGGCCTGGGGGAAGTCCAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCggg                                                                                                                                       <  4:343816/149‑1 (MQ=255)               ggcCTTGCTCGCGGGGCACTTCCATGCCGGCGGCGCGGGCCAGTTCCTCGACGGCCTGGGGGAAGTCCAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCgggg                                                                                                                                      <  6:83030/149‑1 (MQ=255)                  cTTGCTCGCGGGGCACTTCCATGCCGGCGGCGCGGGCCAGTTCCTCGACGGCCTGGGGGAAGTCCAGGTTGTCG‑TGGTCCAGGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAGCTGGTTGTCGGGGCTg                                                                                                                                   >  6:373049/1‑149 (MQ=255)                       tCGCGGGGCACTTCCATGCCGGCGGCGCGGGCCAGTTCCTCGACGGCCTGGGGGAAGTCCAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGt                                                                                                                              >  4:162097/1‑149 (MQ=255)                         gcgGGGCACTTCCATGCCGGCGGCGCGGGCCAGTTCCTCGACGGCCTGGGGGAAGTCCAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTg                                                                                                                             <  4:171801/148‑1 (MQ=255)                         gcgGGGCACTTCCATGCCGGCGGCGCGGGCCAGTTCCTCGACGGCCTGGGGGAAGTCCAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGa                                                                                                                            <  2:88425/149‑1 (MQ=255)                                 cTTCCATGCCGGCGGCGCGGGCCAGTTCCTCGACGGCCTGGGGGAAGTCCAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGAcc                                                                                                                                >  6:357106/1‑137 (MQ=255)                                 cTTCCATGCCGGCGGCGCGGGCCAGTTCCTCGACGGCCTGGGGGAAGTCCAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGAcc                                                                                                                                <  5:357106/137‑1 (MQ=255)                                  ttCCATGCCGGCGGCGCGGGCCAGTTCCTCGACGGCCTGGGGGAAGTCCAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACgggg                                                                                                                   >  3:262912/1‑149 (MQ=255)                                  ttCCATGCCGGCGGCGCGGGCCAGTTCCTCGACGGCCTGGGGGAAGTCCAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACgggg                                                                                                                   >  8:292468/1‑149 (MQ=255)                                      aTGCCGGCGGCGCGGGCCAGTTCCTCGACGGCCTGGGGGAAGTCCAGGTTGTCG‑TGGTCCTTGACAAAGCCCAAGGCGTTGCCACCGGCGCCAGAGCCGAATCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGtttt                                                                                                               <  3:148370/149‑1 (MQ=255)                                      aTGCCGGCGGCGCGGGCCAGTTCCTCGACGGCCTGGGGGAAGTCCAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGtttt                                                                                                               >  7:2703/1‑149 (MQ=255)                                            gcggcgCGGGCCAGTTCCTCGACGGCCTGGGGGAAGTCCAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCttt                                                                                                         <  5:281857/149‑1 (MQ=255)                                             cggcgCGGGCCAGTTCCTCGACGGCCTGGGGGAAGTCCAGGGTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTtg                                                                                                        <  7:313820/149‑1 (MQ=255)                                              ggcgCGGGCCAGTTCCTCGACGGCCTGGGGGAAGTCCAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTtgt                                                                                                       <  2:70633/149‑1 (MQ=255)                                                 gcgGGCCAGTTCCTCGACGGCCTGGGGGAAGTCCAGGTTGTCG‑TGGTCCATGCCAAAGCCCAAGGCGTTGCCACCGGCGCCACCGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTTACCGTGAAGGACGGCGTTTTTTCTTTGTgg                                                                                                     >  7:276128/1‑148 (MQ=255)                                                      ccAGTTCCTCGACGGCCTGGGGGAAGTCCAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGCCGGGGGTTTTTCTTTGTGGAACggg                                                                                               >  6:252831/1‑149 (MQ=255)                                                        aGTTCCTCGACGGCCTGGGGGAAGTCCAGGTTGTCGTTGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACggg                                                                                               >  1:254365/1‑148 (MQ=255)                                                          ttCCTCGACGGCCTGGGGGAAGTCCAGTTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGgcagc                                                                                           <  1:80230/149‑1 (MQ=255)                                                             cTCGACGGCCTGGGGGAAGTCCAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGcagcag                                                                                         <  1:357046/148‑1 (MQ=255)                                                             cTCGACGGCCTGGGGGAAGTCCAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAgg                                                                                        >  1:324513/1‑149 (MQ=255)                                                                gACGGCCTGGGGGAAGTCCAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGcc                                                                                      >  5:157755/1‑148 (MQ=255)                                                                gACGGCCTGGGGGAAGTCCAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGcc                                                                                      >  5:384401/1‑148 (MQ=255)                                                                 aCGGCCTGGGGGAAGTCCAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGa                                                                                    <  3:280531/149‑1 (MQ=255)                                                                    gCCTGGGTGAAGTCCAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGagta                                                                                 <  7:292468/149‑1 (MQ=255)                                                                    gCCTGGGGGAAGTCCAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGagta                                                                                 <  1:323979/149‑1 (MQ=255)                                                                     ccTGGGGGAAGTCCAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGagtag                                                                                >  4:68754/1‑149 (MQ=255)                                                                      cTGGGGGAAGTCCAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGagtagt                                                                               >  2:201257/1‑149 (MQ=255)                                                                      cTGGGGGAAGTCCAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGagtagt                                                                               >  6:60558/1‑149 (MQ=255)                                                                           ggAAGTCCAGGTTGTCG‑TGGTCCGTGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTg                                                                          >  4:62016/1‑149 (MQ=255)                                                                            gAAGTCCAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGc                                                                         >  5:145680/1‑149 (MQ=255)                                                                                tCCAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGt                                                                     <  2:254365/149‑1 (MQ=255)                                                                                 ccAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGtt                                                                    >  8:182791/1‑149 (MQ=255)                                                                                  cAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTtgt                                                                                                       >  2:324519/1‑113 (MQ=255)                                                                                  cAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTtgt                                                                                                       <  1:324519/113‑1 (MQ=255)                                                                                  cAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGttt                                                                   >  3:216495/1‑149 (MQ=255)                                                                                   aGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGtttt                                                                  >  7:257060/1‑149 (MQ=255)                                                                                        gTCG‑TGGTCCCTGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTc                                                              <  3:62016/148‑1 (MQ=255)                                                                                         tCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAg                                                            >  7:12149/1‑149 (MQ=255)                                                                                          cG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCCGc                                                           >  5:214903/1‑149 (MQ=255)                                                                                           g‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGc                                                           <  6:183943/148‑1 (MQ=255)                                                                                               gTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGCTgg                                                        <  2:324513/148‑1 (MQ=255)                                                                                               gTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGCTGGa                                                       <  6:265859/149‑1 (MQ=255)                                                                                               gTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCAACGCCGAAGAAGTAGTAGAAATGCTTGTGGGGGGTGTCCGTGATGGCAGGGGTTTTTTGTTTGTGTAAGGGGCAGAAGGGCGAGTAGTTCTTGCAGGTTTTTTTGAGCTGGa                                                       >  8:161180/1‑149 (MQ=255)                                                                                                 ccATGACAATGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGagt                                                                                  <  5:271345/120‑1 (MQ=255)                                                                                                 ccATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGgc                                                                                              >  3:111967/1‑108 (MQ=255)                                                                                                 ccATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGgc                                                                                              <  4:111967/108‑1 (MQ=255)                                                                                                 ccATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGagt                                                                                  >  6:271345/1‑120 (MQ=255)                                                                                                  cATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCTAGTAGTTCTAGACGGTTTTTTTCAGCTGGAcgc                                                    >  4:99460/1‑149 (MQ=255)                                                                                                   aTGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGCTGGAcgcg                                                   >  3:261014/1‑149 (MQ=255)                                                                                                    tGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGCTGGAcgcgc                                                  <  6:157755/149‑1 (MQ=255)                                                                                                     gACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGa                                                                                    <  3:345166/114‑1 (MQ=255)                                                                                                     gACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGa                                                                                    >  4:345166/1‑114 (MQ=255)                                                                                                      aCAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGCTGGAcgcgcg                                                 <  5:60558/148‑1 (MQ=255)                                                                                                       cAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGttttttt                                                               <  6:277651/133‑1 (MQ=255)                                                                                                       cAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGttttttt                                                               >  5:277651/1‑133 (MQ=255)                                                                                                       cAAAGCCCAAGGCGTTGCCACCGGCGCAACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGAGAAGGACGGCGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGCTGGAcgcgcca                                                >  8:101760/1‑146 (MQ=255)                                                                                                        aaaGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGCTGGACGCGCGAAc                                              >  8:267358/1‑149 (MQ=255)                                                                                                        aaaGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAACGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGCTGGACGCGCGAAc                                              >  6:331927/1‑149 (MQ=255)                                                                                                            cccAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGCTGGACGCGCGAACTcac                                          <  8:12149/149‑1 (MQ=255)                                                                                                              cAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGCTGGACGCGCGAACTcacca                                        >  1:218211/1‑149 (MQ=255)                                                                                                              cAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGCTGGACGCGCGAACTcacca                                        >  1:333388/1‑149 (MQ=255)                                                                                                                  gCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGCTGGACGCGCGAACTCACCACGTc                                    >  7:30248/1‑149 (MQ=255)                                                                                                                    gTTGCCACCGGCGCCACATCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGCTGGACGCGCGAACTCACCACGTcga                                  <  7:331586/149‑1 (MQ=255)                                                                                                                     ttGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGCTGGACGCGCGAACTCACCACGTcgac                                 <  6:37547/149‑1 (MQ=255)                                                                                                                        ccACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGCTGGACGCGCGACCTCACCACGTCGACGAt                              >  4:273872/1‑149 (MQ=255)                                                                                                                        ccACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGACGGTTTTTTTCAGCTGGAAGCGCGAACTCACCACGTCGACGAt                              >  1:282094/1‑149 (MQ=255)                                                                                                                         cACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGCTGGACGCGCGAACTCACCACGTCGACGATg                             >  5:177230/1‑149 (MQ=255)                                                                                                                          aCCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGCTGGACGCGCGAACTCACCACGTCGACGATGt                            >  3:67078/1‑149 (MQ=255)                                                                                                                             ggCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGag                                                                                   >  4:185687/1‑91 (MQ=255)                                                                                                                             ggCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGag                                                                                   <  3:185687/91‑1 (MQ=255)                                                                                                                              gcgcCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGCTGGACGCGCGAACTCACCACGTCGACGATGTCGAg                        >  5:77424/1‑149 (MQ=255)                                                                                                                              gcgcCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGCTGGACGCGCGAACTCACCACGTCGACGATGTCGAg                        >  6:274253/1‑149 (MQ=255)                                                                                                                              gcgcCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGCTGGACGCGCGAACTCACCACGTCGACGATGTCGAg                        <  4:262912/149‑1 (MQ=255)                                                                                                                                gcCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGCTGGACGCGCGAACTCACCACGTCGACGATGTCGAGGc                      <  8:257060/149‑1 (MQ=255)                                                                                                                                 ccACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGCTGGACGCGCGAACTCACCATGTCGACGATGTCGAGGCg                     <  1:201257/149‑1 (MQ=255)                                                                                                                                      cgacaaGCAGTTCTACTACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGCTGGACGCGCGAACTCACCACGTCGACGATGTCGAGGCGGTGGa                >  2:312218/5‑149 (MQ=255)                                                                                                                                      cgacaaGCAGTTCTACTACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTATCTTTGTGGAACGCGCAGCAGGCCGAGTACTTCTTGCCGGTTTTTTTCAGCTGGACGCGCGAACTCACCACGTCGCCGATGTCGAGGCGGTTgc                >  5:283401/5‑148 (MQ=255)                                                                                                                                        cGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGCTGGACGCGCGAACTCACCACGTCGACGATGTCGAGGCGGTTGATc              <  7:267358/149‑1 (MQ=255)                                                                                                                                          aaGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGCTGGACGCGCGAACTCACCACGTCGACGATGTCGAGGCGGTTGATCAg            <  3:68754/149‑1 (MQ=255)                                                                                                                                           aGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTCCGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGCTGGACGCGCGAACTCACCACGTCGACGATGTCGAGGCGGTTGATCAgg           <  4:76930/149‑1 (MQ=255)                                                                                                                                             cAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGCTGGACGCGCGAACTCACCACGTCGACGATGTCGAGGCGGTTGATCAGgtc         >  2:55667/1‑149 (MQ=255)                                                                                                                                              agtagtAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGCTGGACGCGCGAACTCACCACGTCGACGATGTCGAGGCGGTTGATCAGgtcg        <  4:67078/149‑1 (MQ=255)                                                                                                                                                   tagAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGCTGGCCGCGCGAACTCACCACGTCGACGATGTCGAGGCGGTTGATCAGGTCGTCaa    <  7:182791/148‑1 (MQ=255)                                                                                                                                                   tagAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGCTGGACGCGCGAACTCACCACGTCGACGATGTCGAGGCGGTTGATCAGGTCGTCAAt   <  3:99460/149‑1 (MQ=255)                                                                                                                                                    agAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGCTGGACGCGCGAACTCACCACGTCGACGATGTCGAGGCGGTTGATCAGGTCGTCAAt   >  3:261145/1‑148 (MQ=255)                                                                                                                                                     gAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGCTGGACGCGCGAACTCACCACGTCGACGATGTCGAGGCGGTTGATCAGGTCGTCAATg  <  3:162097/148‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                    CTTCTGGTCGCGGCGGCCTTGCTCGCGGGGCACTTCCATGCCGGCGGCGCGGGCCAGTTCCTCGACGGCCTGGGGGAAGTCCAGGTTGTCG‑TGGTCCATGACAAAGCCCAAGGCGTTGCCACCGGCGCCACAGCCGAAGCAGTAGTAGAACTGCTTGTCGGGGCTGACCGTGAAGGACGGGGTTTTTTCTTTGTGGAACGGGCAGCAGGCCGAGTAGTTCTTGCCGGTTTTTTTCAGCTGGACGCGCGAACTCACCACGTCGACGATGTCGAGGCGGTTGATCAGGTCGTCAATG  >  NC_002947/474664‑474958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑