Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	479,493	A→G	10.9%	intergenic (‑25/+31)	cca ← / ← ycgB	multifunctional tRNA nucleotidyltransferase/2',3'‑cyclic phosphodiesterase/2' nucleotidase/phosphatase/type IV piliation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	479,493	0	A	G	10.9%	169.8 / 10.8	64	intergenic (‑25/+31)	cca/ycgB	multifunctional tRNA nucleotidyltransferase/2',3'‑cyclic phosphodiesterase/2' nucleotidase/phosphatase/type IV piliation protein
Reads supporting (aligned to +/- strand):  ref base A (29/28);  new base G (2/5);  total (31/33)
Fisher's exact test for biased strand distribution p-value = 4.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.71e-01

CCATTTGCTAGCATTTCTTCGACGGTGGCGCCCACCACCAGCCAGTCGATATCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCAGAGGTGGTGGATAACAGCCAGGTCCATGCGGCCATAGTCGGCGCTTTCGCTATGCTCGCCGCGCGGCGGCATGTGGTGAGTACTCATCACTTGCTCGCCTTGCACGGTCTC  >  NC_002947/479356‑479637                                                                                                                                          |                                                                                                                                                 ccATTTGCTAGCATTTCTTCGACGGTGGCGCCCACCACCAGCCAGTCGATATCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATGACCGCCGACGGGTTATCCTGt                                                                                                                                       <  7:255918/149‑1 (MQ=255)  cATTTGCTAGCATTTCTTCGACGGTGGCGCCCACCACCAGCCAGTCGATATCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTc                                                                                                                                      >  8:74280/1‑149 (MQ=255)  cATTTGCTAGCATTGCTTCGACGGTGGCGCCCACCACCAGCCAGTCGATATCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTc                                                                                                                                      <  3:144289/149‑1 (MQ=255)    tttGCTAGCATTTCTTCGACGGTGGCGCCCACCACCAGCCAGTCGATATCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTcgg                                                                                                                                    <  7:149417/149‑1 (MQ=255)       gCTAGCCTTTCTTCGCCGGTGGCGCCCACCACCCGCCACGCGCTATCGCTGACAGGGCGGCCGAGCAAACGGGCGCGCACGGCGCCGCCAACTTTGTAGATGGGCATGTGCAGCTCTCCATAACTGCCGACACGATAACCCGTcggcg                                                                                                                                  <  2:175568/148‑1 (MQ=255)       gCTAGCATTTCTTCGACGGTGGCGCCCACCACCAGCCAGTCGATATCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACGGGTTATCCTGTcggca                                                                                                                                  <  1:366079/148‑2 (MQ=255)         tAGCATTTCTTCGACGGTGGCGCCCACCACCAGCCAGTCGATATCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGt                                                                                                                                >  6:330888/1‑148 (MQ=255)         tAGCATTTCTTCGACGGTGGCGCCCACCACCAGCCAGTCGATATCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTc                                                                                                                               >  5:6115/1‑149 (MQ=255)           gCATTTCTTCGACGGTGGCGCCCACCACCAGCCAGTCGATATCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACGGGTTATCCTGTCGGCAGtta                                                                                                                              <  5:218194/148‑3 (MQ=255)              tttCTTCGACGGTGGCGCCCACCACCAGCCAGTCGATATCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACCGCCGACGGGTTATCCTGTCGGCAGTTATgga                                                                                                                          <  3:129052/149‑2 (MQ=38)                tcttcGCCGGTGGCGCCCACCTCCAGCCAGTCGATATCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATgggg                                                                                                                         <  5:53548/148‑1 (MQ=255)                tcttcGACGGTGGCGCCCACCACCAGCCAGTCGATATCGCTGACAGGGCGGCCGAGCAAACGGTCTCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATgggg                                                                                                                         <  3:15744/148‑1 (MQ=255)                tcttcGACGGTGGCGCCCACCACCAGCCAGTCGATATCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGt                                                                                                                        >  1:181064/1‑149 (MQ=255)                  ttcGACGGTGGCGCCCACCACCAGCCAGTCGATATCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTg                                                                                                                      >  4:69100/1‑149 (MQ=255)                  ttcGACGGTGGCGCCCACCACCAGCCAGTCGATATCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTg                                                                                                                      >  6:241913/1‑149 (MQ=255)                   tcGACGGTGGCGCCCACCACCAGCCAGTCGATATCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTg                                                                                                                      >  6:264013/1‑148 (MQ=255)                   tcGACGGTGGCGCCCACCACCAGCCAGTCGATATCGCTGACAGGGCAGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTcggcgg                                                                                                                                 >  7:13232/1‑137 (MQ=255)                   tcGACGGTGGCGCCCACCACCAGCCAGTCGATATCGCTGACAGGGCAGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTcggcgg                                                                                                                                 <  8:13232/137‑1 (MQ=255)                    cGACGGTGGCGCCCACCACCAGCCAGTCGATATCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGtt                                                                                                                       <  7:353846/146‑1 (MQ=255)                    cGACGGTGGCGCCCACCACCAGCCAGTCGATATCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGc                                                                                                                     <  5:330888/148‑1 (MQ=255)                       cGGTGGCGCCCACCACCAGCCAGTCGATATCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCa                                                                                                                 <  3:69100/149‑1 (MQ=255)                        ggTGGCGCCCACCACCAGCCAGTCGATATCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAAc                                                                                                                                           >  3:216867/1‑122 (MQ=255)                        ggTGGCGCCCACCACCAGCCAGTCGATATCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAAc                                                                                                                                           <  4:216867/122‑1 (MQ=255)                         gTGGCGCCCACCACCAGCCAGTCGATATCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCaga                                                                                                               <  5:264013/149‑1 (MQ=255)                          tGGCGCCCACCACCAGCCAGTCGATATCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCagag                                                                                                              <  6:6115/149‑1 (MQ=255)                                    caccaGCCAGTCGATCTCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTc                                                                                                                               >  4:32659/1‑122 (MQ=255)                                     accaGCCAGTCGATATCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATgggg                                                                                                                         <  2:323416/127‑1 (MQ=255)                                     accaGCCAGTCGATATCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATgggg                                                                                                                         >  1:323416/1‑127 (MQ=255)                                     accaGCCAGTCGATATCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCAGAGGTGGTGGATaa                                                                                                   <  2:181064/149‑1 (MQ=255)                                     accaGCCAGTCGATATCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCAGAGGTGGTGGATaa                                                                                                   <  7:321478/149‑1 (MQ=255)                                       cagccagTCGATATCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCAGAGGTGGTGGATAACa                                                                                                 <  5:241913/149‑1 (MQ=255)                                       cagccagTCGATATCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCAGAGGTGGTGGATAACa                                                                                                 <  6:130096/149‑1 (MQ=255)                                         gccagTCGATATCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATGACCGCCGACGGGTTATCCTGTCGGCAGTTATggagag                                                                                                                       >  5:114564/1‑121 (MQ=25)                                         gccagTCGATATCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATGACCGCCGACGGGTTATCCTGTCGGCAGTTATggagag                                                                                                                       <  6:114564/125‑5 (MQ=25)                                                gATATCGCTGACAGGGCGGCCGAGGAAACGGTCGCGCACGGCGCGGCCATCTTTGTAGATGTGCATGTGCAGCTCTCCATACCTGCCGAGAGGATAACCCGTCGGCGGTCATGGGGTTGGATCAGCGGTGGTGGCTAACAGCCAGGTcc                                                                                        >  8:313381/1‑149 (MQ=255)                                                gATATCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCAGAggtggt                                                                                                         >  8:168593/1‑132 (MQ=255)                                                gATATCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCAGAggtggt                                                                                                         <  7:168593/132‑1 (MQ=255)                                                    tCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCAGAGGTGGTGGATAACAGCCAGGTCCATGc                                                                                    <  7:74280/149‑1 (MQ=255)                                                       cTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCAGAGGTGGTGGATAACAGCCAGGTCCATGCGGc                                                                                 >  6:344425/1‑149 (MQ=255)                                                            aGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGGATCAGAGGTGGTGGATAACAGCCAGGTCCATGCGGCCATAg                                                                            >  8:150624/1‑149 (MQ=255)                                                                    cGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCAGAGGTGGTGGATAACAGCCAGGTCCATGCGGCCATAGTCGGCGCt                                                                    >  7:244968/1‑149 (MQ=255)                                                                    cGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCAGAGGTGGTGGATAACAGCCAGGTCCATGCGGCCATAGTCGGCGCt                                                                    >  6:182987/1‑149 (MQ=255)                                                                               tCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCAGAGGTGGTGGATAACAGCCAGGTCCATGCGGCCATAGTCGGCGCTTTCGCTATGCt                                                         >  4:359907/1‑149 (MQ=255)                                                                                          gccgccAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCAGAGGTGGTGGATAACAGCCAGGTCCATGCGGCCATAGTCGGCGCTTTCGCTATGCTCGCCGCgcggc                                              <  3:9194/149‑1 (MQ=255)                                                                                             gccAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCAGAGGTGGTGGATAACAGCCAGGTCCATGCGGCCATAGTCGGCGCTTTCGCTATGCTCGCCGCGcggcgg                                            >  1:356925/1‑148 (MQ=255)                                                                                               cAACTTTGTAGATGTGCATGTGCAGCTCTCAATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCAGAGGTGGTGGATAACAGCCAGGTCCATGCGGCCATAGTCGGCGCTTTCGCTATGCTCGCCGCGCGGCGGCAt                                         >  1:223874/1‑149 (MQ=255)                                                                                               cAACTTTGTAGATGTGCATGTGCAGCTCTCAATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCAGAGGTGGTGGATAACAGCCAGGTCCATGCGGCCATAGTCGGCGCTTTCGCTATGCTCGCCGCGCGGCGGCAt                                         >  1:218508/1‑149 (MQ=255)                                                                                                  cTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCAGAGGTGGTGGATAACAGCCAGGTCCATGCGGCCATAGTCGGCGCTTTCGCTATGCTCGCCGCGCGGCGGCAtgtg                                      <  5:182987/149‑1 (MQ=255)                                                                                                   tttGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCAGAGGTGGTGGATAACAGCCAGGTCCATGCGGCCATAGTCGGCGCTTTCGCTATGCTCGCCGCGCGGCGGCATgtgg                                     >  1:126552/1‑149 (MQ=255)                                                                                                       tAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCAGAGGTGGTGGATAACAGCCAGGTCCATGCGGCCATAGTCGGCGCTTTCGCTATGCTCGCCGCgcggc                                              <  3:100444/136‑1 (MQ=255)                                                                                                       tAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCAGAGGTGGTGGATAACAGCCAGGTCCATGCGGCCATAGTCGGCGCTTTCGCTATGCTCGCCGCgcggc                                              >  4:100444/1‑136 (MQ=255)                                                                                                       tAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCAGAGGTGGTGGATAACAGCCAGGTCCATGCGGCCATAGTCGGCGCTTTCGCTATGCTCGCCGCGCGGCGGCATGTGGTGa                                  <  3:359907/148‑1 (MQ=255)                                                                                                               catgtgcaGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCAGAGGTGGTGGATAACAGCCAGGTCCATGCGGCCATAGTCGGCGCTTTCGCTATGCTCGCCGCGCGGCGGCATGTGGTGAGTACtcat                          >  4:336262/1‑148 (MQ=255)                                                                                                                   cgcaGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTTCATCATAGTTGGTGGATAACAGCAAGTTCCATGATTCCATAGTCGTCGCTTTCTCTATGCTCGCCGTGCGGCGGCATGTGATGAGTACTcaccacat                     <  2:115785/148‑3 (MQ=255)                                                                                                                     caGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCAGAGGTGGTGGATAACAGCCAGGTCCATGCGGCCATAGTCGGCGCTTTCGCTATGCTCGCCGCGCGGCGGCATGTGGTGAGTACTCATCACTTGc                   <  2:126552/149‑1 (MQ=255)                                                                                                                           cccATGACCGCCGACGGGTTATCCTGTCGGCAGTTATGGGGTTGCATCAGAGGTGGTGGATAACAGCCAGGTCCATGCGGCCATAGTCGGCGCTTTCGCTATGCTCGCCGCGCGGCGGCATGTGGTGAGTACTCATCACTTGCTCGCCt             >  8:52081/2‑149 (MQ=38)                                                                                                                             cATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCAGAGGTGGTGGATAACAGCCAGGTCCATGCGGCCATAGTCGGCGCTTTCGCTATGCTCGCCGCGCGGCGGCATgtggt                                    >  6:232665/1‑124 (MQ=255)                                                                                                                             cATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCAGAGGTGGTGGATAACAGCCAGGTCCATGCGGCCATAGTCGGCGCTTTCGCTATGCTCGCCGCGCGGCGGCATgtggt                                    <  5:232665/124‑1 (MQ=255)                                                                                                                             cATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCAGAGGTGGTGGATAACAGCCAGGTCCATGCGGCCATAGTCGGCGCTTTCGCTATGCTCGCCGCGCGGCGGCATGTGGTGAGTACTCATCACTTGCTCGCCtt            <  7:150624/148‑1 (MQ=255)                                                                                                                             cATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCAGAGGTGGTGGATAACAGCCAGGTCCATGCGGCCATAGTCGGCGCTTTCGCTATGCTCGCCGCGCGGCGGCATGTGGTGAGTACTCATCACTTGCTCGCCTTg           >  8:840/1‑149 (MQ=255)                                                                                                                                  cTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCAGAGGTGGTGGATAACAGCCAGGTCCATGCGGCCATAGTCGGCGCTTTCGCTATGCTCGCCGCGCGGCGGCATGTGGTGAGTACTCATCACTTGCTCGCCTTGCACgg      >  1:215960/1‑149 (MQ=255)                                                                                                                                   tGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCAGAGGTGGTGGATAACAGCCAGGTCCATGCGGCCATAGTCGGCGCTTTCGCTATGCTCGCCGCGCGGCGGCATGTGGTGAGTACTCATCACTTGCTCGCCTTGCACGGt     >  5:40031/1‑149 (MQ=255)                                                                                                                                     ccGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCAGAGGTGGTGGATAACAGCCAGGTCCATGCGGCCATAGTCGGCGCTTTCGCTATGCTCGCCGCGCGGCGGCATGTGGTGAGTACTCATCACTTGCTCGCCTTGCACTGtct   >  8:230522/1‑149 (MQ=255)                                                                                                                                      cGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCAGAGGTGGTGGATAACAGCCAGGTCCATGCGGCCATAGTCGGCGCTTTCGCTATGCTCGCCGCGCGGCGGCATGTGGTGAGTACTCATCACTTGCTCGCCTTGCACGGtctc  <  8:244968/149‑1 (MQ=255)                                                                                                                                          |                                                                                                                                                 CCATTTGCTAGCATTTCTTCGACGGTGGCGCCCACCACCAGCCAGTCGATATCGCTGACAGGGCGGCCGAGCAAACGGTCGCGCACGGCGCCGCCAACTTTGTAGATGTGCATGTGCAGCTCTCCATAACTGCCGACAGGATAACCCGTCGGCGGTCATGGGGTTGCATCAGAGGTGGTGGATAACAGCCAGGTCCATGCGGCCATAGTCGGCGCTTTCGCTATGCTCGCCGCGCGGCGGCATGTGGTGAGTACTCATCACTTGCTCGCCTTGCACGGTCTC  >  NC_002947/479356‑479637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑