Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 521,212 T→C 8.9% E28E (GAA→GAG) ‡ anmK ← anhydro‑N‑acetylmuramic acid kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947521,2120TC 8.9% 223.7 / 9.1 80E28E (GAA→GAG) ‡anmKanhydro‑N‑acetylmuramic acid kinase
Reads supporting (aligned to +/- strand):  ref base T (34/38);  new base C (4/3);  total (39/41)
Fisher's exact test for biased strand distribution p-value = 7.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGGCGGATACCTTCGCCGGCCAGGCTGGCCCAGCGGTTTTCCGCCAGTGCTGCACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAGTAGAGCGCCATGGCTTAGCGCTTGTTCGCTGCGAGCAGGGTGGCCTTTTCCTGATCCATACGGGCAATCAGCGGCT  >  NC_002947/521068‑521360
                                                                                                                                                |                                                                                                                                                    
ttGGCGGATACCTTCGCCGGCCAGGCTGGCCCAGCGGTTTTCCGCCAGTGCTGCACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCaa                                                                                                                                                  <  8:150048/149‑1 (MQ=255)
   gCGGATACCTTCGCCGGCCAGGCTGGCCCAGCGGTTTTCCGCCAGTGCTGCACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGTTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAgc                                                                                                                                                <  3:39976/148‑1 (MQ=255)
   gCGGATACCTTCGCCGGCCAGGCTGGCCCAGCGGTTTTCCGCCAGTGCTGCACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAgc                                                                                                                                                <  3:260383/148‑1 (MQ=255)
          ccTTCGCCGGCCAGGCTGGCCCAGCGGTTTTCCGCCAGTGCTGCACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGcc                                                                                                                                        <  8:267189/149‑1 (MQ=255)
           cTTCGCCGGCCAGGCTGGCCCAGCGGTTTTCCGCCAGTGCTGCACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCt                                                                                                                                       >  5:1624/1‑149 (MQ=255)
           cTTCGCCGGCCAGGCTGGCCCAGCGGTTTTCCGCCAGTGCTGCACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCt                                                                                                                                       >  8:176832/1‑149 (MQ=255)
           cTTCGCCGGCCAGGCTGGCCCAGCGGTTTTCCGCCAGTGCTGCACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCt                                                                                                                                       >  4:312101/1‑149 (MQ=255)
           cTTCGCCGGCCAGGCTGGCCCAGCGGTTTTCCGCCAGTGCTGCACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCt                                                                                                                                       >  1:186238/1‑149 (MQ=255)
           cTTCGCCGGCCAGGCTGGCCCAGCGGTTTTCCGCCAGTGCTGCACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGCTTGAACTGCTCGc                                                                                                                                         <  1:65716/147‑1 (MQ=255)
           cTTCGCCGGCCAGGCTGGCCCAGCGGTTTTCCGCCAGTGCTGCACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGCTTGAACTGCTCGc                                                                                                                                         >  2:65716/1‑147 (MQ=255)
           cTTCGCCGGCCAGGCTGGCCCAGCGGTTTTCCGCCAGTGCTGCACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGCGCCAGCAGGTCCTGGCGCACGTCCGGGGGCATGGGCAGGAAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCt                                                                                                                                       >  3:133941/1‑149 (MQ=255)
               gCCGGCCAGGCTGGCCCAGCGGTTTTCCGCCAGTGCTGCACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGtt                                                                                                                                   <  7:176832/149‑1 (MQ=255)
               gCCGGCCAGGCTGGCCCAGCGGTTTTCCGCCAGTGCTGCACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGtt                                                                                                                                   >  5:349714/1‑149 (MQ=255)
               gCCGGCCAGGCTAGCCCAGCGGTTTTCCGCCAGTGCTGCACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGt                                                                                                                                    >  6:304160/1‑148 (MQ=255)
                ccGGCCAGGCTGGCCCAGCGGTTTTCCGCCAGTGCTGCACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTc                                                                                                                                  <  4:198891/149‑1 (MQ=255)
                ccGGCCAGGCTGGCCCAGCGGTTTTCCGCCAGTGCTGCACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTc                                                                                                                                  >  5:333899/1‑149 (MQ=255)
                   gCCAGGCTGGCCCAGCGGTTTTCCGCCAGTGCTGCACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGAc                                                                                                                               <  8:145361/149‑1 (MQ=255)
                   gCCAGGCTGGCCCAGCGGTTTTCCGCCAGTGCTGCACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGCTTGAACTGCTCg                                                                                                                                          >  7:145045/1‑138 (MQ=255)
                   gCCAGGCTGGCCCAGCGGTTTTCCGCCAGTGCTGCACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGCTTGAACTGCTCg                                                                                                                                          <  8:145045/138‑1 (MQ=255)
                        gCTGGCCCAGCGGTTTTCCGCCAGTGCTGCACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAggg                                                                                                                          >  4:349943/1‑149 (MQ=255)
                        gCTGGCCCAGCGGTTTTCCGCCAGTGCTGCACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAggg                                                                                                                          <  1:105951/149‑1 (MQ=255)
                         cTGGCCCAGCGGTTTTCCGCCAGTGCTGCACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCCTGGTCGAACAAGGc                                                                                                                         <  4:319799/149‑1 (MQ=255)
                            gCCCAGCGGTTTTCCGCCAGTGCTGCTCGGTCAATCTCGTCTGGGCCGATGCTGCACAGGGGCAGCTGGTTGTGGCGCAGGTCCGTGGGCATGTGCAGGTAGTGGGTGGCTAGAAGTTCTAGCTGCTCGCCTTGTTTGACCAGGGCgtt                                                                                                                      <  4:133941/149‑3 (MQ=255)
                            gCCCAGCGGTTTTCCGCCAGTGCTGCACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCg                                                                                                                        <  3:312101/147‑1 (MQ=255)
                             cccAGCGGTTTTCCGCCAGTGCTGCACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGACCTGGCGCAGGGCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGata                                                                                                                     >  3:18538/1‑149 (MQ=255)
                              ccAGCGGTTTTCCGCCAGTGCTGCACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGatat                                                                                                                    >  4:116324/1‑149 (MQ=255)
                                aGCGGTTTTCCGCCAGTGCTGCACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATc                                                                                                                   >  6:244038/1‑148 (MQ=255)
                                  cGGTTTTCCGCCAGTGCTGCACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAg                                                                                                                <  8:217639/149‑1 (MQ=255)
                                     ttttCCGCCAGTGCTGCACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGCCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCTGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGcc                                                                                                             >  6:337560/1‑149 (MQ=255)
                                           gCCAGTGCTGCACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAg                                                                                                       <  2:186238/149‑1 (MQ=255)
                                             cAGTGCTGCACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGc                                                                                                     <  3:349943/149‑1 (MQ=255)
                                              aGTGCTGCACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCt                                                                                                    <  5:304160/149‑1 (MQ=255)
                                               gTGCTGCACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGACCTGGCGCAGGGCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTg                                                                                                   <  4:18538/149‑1 (MQ=255)
                                                tgctgcACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTgg                                                                                                  >  8:46650/1‑149 (MQ=255)
                                                    gcACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGcc                                                                                              >  8:64470/1‑149 (MQ=255)
                                                    gcACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGAGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAgg                                                                                                                           <  4:275902/120‑1 (MQ=255)
                                                    gcACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGAGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAgg                                                                                                                           >  3:275902/1‑120 (MQ=255)
                                                      aCGGGCAATCTCGTCAGGGCCGCTGCTGCACAGAGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCgg                                                                                            >  2:173298/1‑149 (MQ=255)
                                                          gCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACAt                                                                                        <  2:321675/149‑1 (MQ=255)
                                                          gCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACAt                                                                                        >  5:35466/1‑149 (MQ=255)
                                                               ctcGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCAccc                                                                                   >  2:319395/1‑149 (MQ=255)
                                                                  gTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCAccccc                                                                                 >  8:151789/1‑148 (MQ=255)
                                                                    cAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAg                                                                              <  3:116324/149‑1 (MQ=255)
                                                                     aGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAg                                                                              >  1:11678/1‑148 (MQ=255)
                                                                      gggCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAGTa                                                                            <  5:157726/149‑1 (MQ=255)
                                                                               ctgcACAGGGCCAGCAGGTCCTGGTGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAGTAGAGCGCCAt                                                                   >  6:9238/1‑149 (MQ=255)
                                                                                  cacaGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGGATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCCCCCCCCAGTAGCGCGCCATGGc                                                                >  6:187805/1‑149 (MQ=255)
                                                                                  cacaGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAGTAGAGCGCCATgg                                                                 <  7:151789/148‑1 (MQ=255)
                                                                                       ggCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAGTAGAGCGCCATGGCTTAgc                                                           >  4:208210/1‑149 (MQ=255)
                                                                                        gCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAGTAGAGCGCCATGGCTTAgcg                                                          >  1:146614/1‑149 (MQ=255)
                                                                                         ccAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAGTAGAGCGCCATGGCTTAgcgc                                                         <  1:319395/149‑1 (MQ=255)
                                                                                         ccAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAGTAGAGCGCCATGGCTTAgcgc                                                         <  7:64470/149‑1 (MQ=255)
                                                                                         ccAGCAGGACCTCGCGCAGGTCCGGGGGCATGGGCAGGAAGTGGGTGGCGCGAAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCAAGGACGCTCGTCCCGGACATCACCCCCAAGTAGAGCGCCAACGCTAAgcc                                                          >  4:15336/1‑147 (MQ=255)
                                                                                                gTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAGTAGAGCGCCATGGCTTAGCGCTTGTTCg                                                  >  3:224393/1‑149 (MQ=255)
                                                                                                 tCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAGTAGAGCGCCATGGCTTAGCGCTTGTTCGc                                                 >  6:289885/1‑149 (MQ=255)
                                                                                                      gcgcAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAgc                                                                                                                                                >  4:54392/1‑49 (MQ=255)
                                                                                                      gcgcAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAgc                                                                                                                                                <  3:54392/49‑1 (MQ=255)
                                                                                                      gcgcAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAGTAGAGCGCCATGGCTTAGCGCTTGTTCGCTGc                                              <  6:333899/147‑1 (MQ=255)
                                                                                                        gcAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAGTAGAGCGCCATGGCTTAGCGCTTGTTCGCTGCGAg                                           >  3:330310/1‑148 (MQ=255)
                                                                                                        gcAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGATCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAGTAGAGCGCCATGGCTTAGCGCTTGTTCGCTGCGAg                                           >  5:355798/1‑148 (MQ=255)
                                                                                                           ggTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAGTAGAGCGCCATGGCTTAGCGCTTGTTCGCTGCGAGCAgg                                       <  6:1624/149‑1 (MQ=255)
                                                                                                            gTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCAACCCCAAGTAGAGCGCCATGGCTTAGCGCTTGTTCGCTGCGAGCAggg                                      >  3:8024/1‑149 (MQ=255)
                                                                                                                 ggggCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAGTAGAGCGCCATGGCTTAGCGCTTGTTCGCTGCGAGCAGGGTGGcc                                 <  4:224393/149‑1 (MQ=255)
                                                                                                                      aTGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAGTAGAGCGCCATGGCTTAgc                                                           <  6:45085/118‑1 (MQ=255)
                                                                                                                      aTGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAGTAGAGCGCCATGGCTTAgc                                                           >  5:45085/1‑118 (MQ=255)
                                                                                                                      aTGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAGTAGAGCGCCATGGCTTAGCGCTTGTTCGCTGCGAGCAGGGTGGCCTTTTc                            <  5:96264/149‑1 (MQ=255)
                                                                                                                      aTGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAGTAGAGCGCCATGGCTTAGCGCTTGTTCGCTGCGAGCAGGGTGGCCTTTTc                            <  5:244038/149‑1 (MQ=255)
                                                                                                                      aTGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAGTAGAGCGCCATGGCTTAGCGCTTGTTCGCTGCGAGCAGGGTGGCCTTTTc                            <  7:297467/149‑1 (MQ=255)
                                                                                                                          gCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAGTAGAGCGCCATGGCTTAGCGCTTGTTCGCTGCGAGCAGGGTGGCCTTTTCCTGa                        <  7:46650/149‑1 (MQ=255)
                                                                                                                          gCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCGTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAGTAGAGCGCCATGGCTTAGCGCTTGTTCGCTGCGAGCAGGGTGGCCTTTTCCTGa                        <  5:63709/149‑1 (MQ=255)
                                                                                                                               tAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCATGCCATCGAGGCTGGTGCCGGACATCACCCCCAAGTAGAGCGCCATGGCTTAGCGCTTGTTCGCTGCGAGCAGGGTGGCCTTTTCCTGATCCAt                   <  3:208210/149‑1 (MQ=255)
                                                                                                                                aGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAGTAGAGCGCCATGGCTTAGCGCTTGTTCGCTGCGAGCAGGGTGGCCTTTTCCTGATCCATa                  >  6:329243/1‑149 (MQ=255)
                                                                                                                                    ggtggCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAGTAGAGCGCCATGGCTTAGCGCTTGTTCGCTGCGAGCAGGGTGGCCTTTTCCTGATCCATACggg              <  4:330310/149‑1 (MQ=255)
                                                                                                                                    ggtggCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAGTAGAGCGCCATGGCTTAGCGCTTGTTCGCTGCGAGCAGGGTGGCCTTTTCCTGATCCATACgg               <  2:11678/148‑1 (MQ=255)
                                                                                                                                     aaggCGAGCAGCTTGAACTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAGTAGAGCGCCATGGCTTAGCGCTTGTTCGCTGCGAGCAGGGTGGCCTTTTCCTGATCCATACggg              >  7:48733/3‑148 (MQ=255)
                                                                                                                                      aggCGAGCAGCTTGAACTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAGTAGAGCGCCATGGCTTAGCGCTTGTTCGCTGCGAGCAGGGTGGCCTTTTCCTGATCCATACGGGCa            <  8:48733/148‑1 (MQ=255)
                                                                                                                                       ggCGAGCAGCTTGAACTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAGTAGAGCGCCATGGCTTAGCGCTTGTTCGCTGCGAGCAGGGTGGCCTTTTCCTGATCCATACGGGCaa           >  2:352504/1‑149 (MQ=255)
                                                                                                                                        gCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAGTAGAGCGCCATGGCTTAGCGCTTGTTCGCTGCGAGCa                                         <  7:314155/118‑1 (MQ=255)
                                                                                                                                        gCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAGTAGAGCGCCATGGCTTAGCGCTTGTTCGCTGCGAGCa                                         >  8:314155/1‑118 (MQ=255)
                                                                                                                                             cAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAGTAGAGCGCCATGGCTTAGCGCTTGTTCGCTGCGAGCAGGGTGGCCTTTTCCTGATCCATACGGGCAATCAGCg     <  5:289885/149‑1 (MQ=255)
                                                                                                                                                ttCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCTGACATCACCCCCAAGTAGAGCTCCATGTCTTAGCGCTTGTTCGCTGCTATCAGGGTGGCCTTTTCCTGATCCATACGGTCAAt          <  3:295913/141‑1 (MQ=255)
                                                                                                                                                ttCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAGTAGAGCGCCATGGCTTAGCGCTTGTTCGCTGCGAGCAGGGTGGCCTTTTCCTGATCCATACGGGCAATCAGCggct  >  8:205035/1‑149 (MQ=255)
                                                                                                                                                ttCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCACAAGTAGAGAGCCATGGCTTAGAGATTGTTCGCTGCGAGCAGGGTGTCCTTTTCATGATACATACTGGCAAt          >  4:295913/1‑141 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
TTGGCGGATACCTTCGCCGGCCAGGCTGGCCCAGCGGTTTTCCGCCAGTGCTGCACGGGCAATCTCGTCAGGGCCGCTGCTGCACAGGGCCAGCAGGTCCTGGCGCAGGTCCGGGGGCATGGGCAGGTAGTGGGTGGCGAGCAGTTCAAGCTGCTCGCCTTGTTCGACCAGGGCGATATCCAGGCCATCGAGGCTGGTGCCGGACATCACCCCCAAGTAGAGCGCCATGGCTTAGCGCTTGTTCGCTGCGAGCAGGGTGGCCTTTTCCTGATCCATACGGGCAATCAGCGGCT  >  NC_002947/521068‑521360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑