Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 539,941 T→G 6.4% R773R (CGT→CGG)  rpoB → DNA‑directed RNA polymerase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947539,9410TG 6.4% 245.1 / 6.1 78R773R (CGT→CGG) rpoBDNA‑directed RNA polymerase subunit beta
Reads supporting (aligned to +/- strand):  ref base T (41/32);  new base G (2/3);  total (43/35)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01

GGTGTGATCGACTCGGTCGACGCCAGCCGTATCGTTGTTCGCGTTGCCGACGACGAAGTGGAAACCGGCGAAGCGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCGTGGAACGGCTTCAACTTCGAAGACTCCATCTGC  >  NC_002947/539801‑540088
                                                                                                                                            |                                                                                                                                                   
ggtgGGATCGACTCGTTCGACGCCAGCCGTATCGTTGTTCGCGTTGCCGACGACGAAGTGGAAACCGGCGAAGCGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGt                                                                                                                                             <  6:209132/149‑1 (MQ=255)
  tgtgATCGACTCGGTCGACGCCAGCCGTATCGTTGTTCGCGTTGCCGACGACGAAGTGGAAACCGGCGAAGCGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGa                                                                                                                                           <  1:131999/149‑1 (MQ=255)
    tgATCGACTCGGTCGACGCCAGCCGTATCGTTGTTCGCGTTGCCGACGACGAAGTGGAAACCGGCGAAGCGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAg                                                                                                                                          >  4:332503/1‑148 (MQ=255)
    tgATCGACTCGGTCGACGCAAGCCGTATCGTTGTTCGCGTTGCCGACGACGAAGTGGAAACCGGCGAAGCGGGTGTGGATATCTACAACCTGACAAAGTACACCCGTTCGAACAAGAACACCTGCATCAACCAGCGTCCGATGGTGAg                                                                                                                                          >  6:138490/1‑148 (MQ=255)
      aTCGACTCGGTCGACGCCAGCCGTATCGTTGTTCGCGTTGCCGACGACGAAGTGGAAACCGGCGAAGCGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGGACGCTGGttg                                                                                                                                           <  8:72977/145‑3 (MQ=255)
      aTCGACTCGGTCGACGCCAGCCGTATCGTTGTTCGCGTTGCCGACGACGAAGTGGAAACCGGCGAAGCGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGGACGCTGGttg                                                                                                                                           >  7:72977/1‑143 (MQ=255)
       tCGACTCGGTCGACGCCAGCCTTATCGTTGTTCGCGTTGCCGACGACGAAGTGGAAACCGGCGAAGCGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCaa                                                                                                                                       <  2:342976/148‑1 (MQ=255)
       tCGACTCGGTCGACGCCAGCCGTATGGTTGTTCGCGTTGCCGACGACGAAGTGGAAACCGGCGAAGCGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGGACGCTGGttgatgca                                                                                                                                      <  3:269232/149‑8 (MQ=255)
       tCGACTCGGTCGACGCCAGCCGTATCGTTGTTCGCGTTGCCGACGACGAAGTGGAAACCGGCGAAGCGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGGACGCTGGttgatgca                                                                                                                                      <  5:222277/149‑8 (MQ=255)
        cGACTCGGTCGACGCCAGCCGTATCGTTGTTCGCGTTGCCGACGACGAAGTGGAAACCGGCGAAGCGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAgg                                                                                                                                     >  7:99171/1‑149 (MQ=255)
          aCTCGGTCGACGCCAGCCGTATCGTTGTTCGCGTTGCCGACGACGAAGTGGAAACCGGCGAAGCGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGt                                                                                                                                   <  8:323882/149‑1 (MQ=255)
          aCTCGGTCGACGCCAGCCGTATCGTTGTTCGCGTTGCCGACGACGAAGTGGAAACCGGCGAAGCGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGt                                                                                                                                   <  8:323881/149‑1 (MQ=255)
           cTCGGTCGACGCCAGCCGTATCGTTGTTCGCGTTGCCGACGACGAAGTGGAAACCGGCGAAGCGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGt                                                                                                                                   >  7:43429/1‑148 (MQ=255)
                      ccAGCCGTATCGTTGTTCGCGTTGCCGACGACGAAGTGGAAACCGGCGAAGCGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAg                                                                                                                       <  3:332503/149‑1 (MQ=255)
                           cGTATCGTTGTTCGCGTTGCCGACGACGAAGTGGAAACCGGCGAAGCGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGc                                                                                                                                                 >  1:332943/1‑118 (MQ=255)
                           cGTATCGTTGTTCGCGTTGCCGACGACGAAGTGGAAACCGGCGAAGCGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGc                                                                                                                                                 <  2:332943/118‑1 (MQ=255)
                                     ttCGCGTTGCCGACGACGAAGTGGAAACCGGCGAAGCGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGAcatcat                                                                                                         <  5:83668/148‑1 (MQ=255)
                                         cgTTGCCGACGACGAAGTGGAAACCGGCGAAGCGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCg                                                                                                    <  8:99171/149‑1 (MQ=255)
                                              ccGACGACGAAGTGGAAACCGGCGAAGCGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGc                                                                                               <  5:55397/149‑1 (MQ=255)
                                               cgacgacgaAGTGGAAACCGGCGAAGCGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGcc                                                                                              >  2:125567/1‑149 (MQ=255)
                                                         gTGGAAACCGGCGAAGCGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGtt                                                                                                                          >  4:196413/1‑111 (MQ=255)
                                                         gTGGAAACCGGCGAAGCGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGtt                                                                                                                          <  3:196413/111‑1 (MQ=255)
                                                              aaCCGGCGAAGCGGGTGTGGATATCTACATCCTGACCAAGTACACCCGTTCGAAGCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGTGTGACAAGGTTCAGCGTGTTGACATCATGGCCGACGGCCCGTCCACCGACATgg                                                                               <  5:138490/149‑1 (MQ=255)
                                                              aaCCGGCGAAGCGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATgg                                                                               <  3:45513/149‑1 (MQ=255)
                                                              aaCCGGCGAAGCGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATgg                                                                               <  8:43429/149‑1 (MQ=255)
                                                                     gAAGCGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCt                                                                        >  8:13993/1‑149 (MQ=255)
                                                                     gAAGAGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAAAAAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTAAGCGTGGTGACATCATGTCCGACGGCCCGTCCACCGACATGGTTGAGCt                                                                        >  6:27351/1‑149 (MQ=255)
                                                                            gtgtGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCt                                                                        <  1:367901/142‑1 (MQ=255)
                                                                            gtgtGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCt                                                                        >  2:367901/1‑142 (MQ=255)
                                                                                 gATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACgg                                                                                                <  6:319271/113‑1 (MQ=255)
                                                                                 gATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACgg                                                                                                >  5:319271/1‑113 (MQ=255)
                                                                                  atatCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCTGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAg                                                           <  4:363181/149‑1 (MQ=255)
                                                                                    atCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGaa                                                         >  3:58498/1‑149 (MQ=255)
                                                                                      cTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTGAGCGTGGTGCCATCATGGCCGACGGCCCGGCCCCGGACATGGGGGAGGTGGCTGGGGGTcggcgcg                                                       >  4:306102/1‑143 (MQ=255)
                                                                                       tACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGa                                                                           <  2:194532/128‑1 (MQ=255)
                                                                                       tACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGa                                                                           >  1:194532/1‑128 (MQ=255)
                                                                                          aaCCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATgcg                                                   >  2:49588/1‑149 (MQ=255)
                                                                                           aCCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATgcg                                                   >  2:202784/1‑148 (MQ=255)
                                                                                           aCCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTACGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCACGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATgcg                                                   >  3:193585/1‑148 (MQ=255)
                                                                                            ccTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGaa                                                         <  8:178074/141‑1 (MQ=255)
                                                                                            ccTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGaa                                                         >  7:178074/1‑141 (MQ=255)
                                                                                            ccTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCa                                                 <  3:282650/149‑1 (MQ=255)
                                                                                             cTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGACCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCAt                                                >  2:40121/1‑149 (MQ=255)
                                                                                                aCCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGCGTCAGAACATGCGCATcgc                                             >  4:328343/1‑149 (MQ=255)
                                                                                                  cAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGt                                           >  4:328756/1‑149 (MQ=255)
                                                                                                  cAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGGGACCTCATGGCCGACGGACCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGt                                           >  6:290421/1‑149 (MQ=255)
                                                                                                     gTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCa                                        <  4:58498/149‑1 (MQ=255)
                                                                                                        cacCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACGTGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATg                                      <  3:293590/148‑1 (MQ=255)
                                                                                                          cccGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGct                                                                    >  6:77387/1‑116 (MQ=255)
                                                                                                          cccGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGct                                                                    <  5:77387/116‑1 (MQ=255)
                                                                                                          cccGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCg                                   >  2:223614/1‑149 (MQ=255)
                                                                                                           ccGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCGt                                  >  1:339078/1‑149 (MQ=255)
                                                                                                           ccGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCGt                                  >  1:4720/1‑149 (MQ=255)
                                                                                                                 gAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCGTGGAACg                            >  8:89975/1‑149 (MQ=255)
                                                                                                                   aCCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCGTGGAACGGc                          >  1:131781/1‑149 (MQ=255)
                                                                                                                      aGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCGTGGAACGGCtt                        >  6:128897/1‑148 (MQ=255)
                                                                                                                       gAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCGTGGAACGGCTTc                       >  2:105192/1‑148 (MQ=255)
                                                                                                                        aaCACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCGTGGAACGGCTTCaa                     >  2:246287/1‑149 (MQ=255)
                                                                                                                           acCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCGTg                                 >  7:117218/1‑134 (MQ=255)
                                                                                                                           acCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCGTg                                 <  8:117218/134‑1 (MQ=255)
                                                                                                                            ccTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCTTGTTGAAATCATGGCCGACTGCCCGTCCACCGACTTGGGTGAGCTGGCTCTGGGGCAGAACATGCGCATCGCGTTCATGGCGTGGAACGGCTTCAACTTc                 <  5:27351/149‑1 (MQ=255)
                                                                                                                            ccTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGGCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCGTGGAACGGCTTCAACTTc                 <  1:40121/149‑1 (MQ=255)
                                                                                                                             cTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCGTGGAACGGCTTCAACTTc                 >  2:271833/1‑148 (MQ=255)
                                                                                                                             cTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCGTGGAACGGCTTCAACTTc                 >  2:27716/1‑148 (MQ=255)
                                                                                                                             cTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCGTGGAACGGCTTCAACTTCg                <  3:328756/149‑1 (MQ=255)
                                                                                                                              tGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCGTGGAACGGCTTCAACTTCg                >  5:172161/1‑148 (MQ=255)
                                                                                                                                cATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCGTGGAACGGCTTCAACTTCg                >  1:267143/1‑146 (MQ=255)
                                                                                                                                cATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGAAAGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCGTGGAACGGCTTCAACTTCg                >  1:297880/1‑146 (MQ=255)
                                                                                                                                 aTCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCGTGGAACGGc                          >  5:116503/1‑135 (MQ=255)
                                                                                                                                 aTCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCGTGGAACGGc                          <  6:116503/135‑1 (MQ=255)
                                                                                                                                  tCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCGTGGAACGGCTTCAACTTCGAAGAc           <  1:202784/149‑1 (MQ=255)
                                                                                                                                   tcaCCAGCGGACGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCGTGGAACGGCTTCAACTTCGAAGACt          >  1:251430/3‑149 (MQ=255)
                                                                                                                                    aaCCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCGTGGAACGGCTTCAACTTCGAAGACt          <  7:86770/148‑1 (MQ=255)
                                                                                                                                     aCCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCGTGGAACGGCTTCAACTTCGAAGCCTc         >  3:172107/1‑148 (MQ=255)
                                                                                                                                       cAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCGTGGAACGGCTTCAACTTCGAAGACTCCa       >  8:76476/1‑148 (MQ=255)
                                                                                                                                       cAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCGTGGAACGGCTTCAACTTCGAAGACTCCAt      >  4:355151/1‑149 (MQ=255)
                                                                                                                                           gTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCGTGGAACGGCTTCAACTTCGAAGACTCCATCTGc  <  2:131781/149‑1 (MQ=255)
                                                                                                                                           gTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCGTGGAACGGCTTCAACTTCGAAGACTCCATCTGc  <  3:306102/149‑1 (MQ=255)
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GGTGTGATCGACTCGGTCGACGCCAGCCGTATCGTTGTTCGCGTTGCCGACGACGAAGTGGAAACCGGCGAAGCGGGTGTGGATATCTACAACCTGACCAAGTACACCCGTTCGAACCAGAACACCTGCATCAACCAGCGTCCGCTGGTGAGCAAGGGTGACAAGGTTCAGCGTGGTGACATCATGGCCGACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCGTGGAACGGCTTCAACTTCGAAGACTCCATCTGC  >  NC_002947/539801‑540088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑