Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 543,727 A→G 8.3% N656S (AAC→AGC)  rpoC → DNA‑directed RNA polymerase subunit beta'

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947543,7270AG 8.3% 204.2 / 7.2 72N656S (AAC→AGC) rpoCDNA‑directed RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base A (33/33);  new base G (3/3);  total (36/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.51e-01

TACCGCGTGGTTGGTCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACCTCTCGAAAGAGAA  >  NC_002947/543585‑543874
                                                                                                                                              |                                                                                                                                                    
tACCGCGTGGTTGGTCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTAc                                                                                                                                                >  7:295988/1‑149 (MQ=255)
          ttggtCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAGCGTTACCGAtgatgcg                                                                                                                                      <  5:354928/149‑5 (MQ=255)
          ttggtCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCAGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGAtgaagtg                                                                                                                                      <  3:297317/149‑1 (MQ=255)
              tCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGAtgaagtgaag                                                                                                                                   >  2:236193/1‑148 (MQ=255)
                tGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAGCGTTACCGAtga                                                                                                                                          <  6:383254/139‑1 (MQ=255)
                tGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAGCGTTACCGAtga                                                                                                                                          >  5:383254/1‑139 (MQ=255)
                tGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGa                                                                                                                                 >  1:16493/1‑148 (MQ=255)
                tGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGa                                                                                                                                 >  4:60994/1‑148 (MQ=255)
                  aaaGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGaaa                                                                                                                               <  1:236193/148‑1 (MQ=255)
                     gagaCCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATc                                                                                                                             >  6:349014/1‑147 (MQ=255)
                         ccGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGc                                                                                                                       <  8:298301/149‑1 (MQ=255)
                          cGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGc                                                                                                                       >  4:141328/1‑148 (MQ=255)
                                 ttCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGc                                                                                                               >  8:118002/1‑149 (MQ=255)
                                       gACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑Cgg                                                                                                        <  8:80190/149‑1 (MQ=255)
                                          cAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCt                                                                                                     <  1:279078/149‑1 (MQ=255)
                                            aCTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTgg                                                                                                   >  3:259765/1‑149 (MQ=255)
                                             cTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGt                                                                                                  <  2:300603/149‑1 (MQ=255)
                                             cTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGt                                                                                                  <  5:292195/149‑1 (MQ=255)
                                                aTGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTaa                                                                                                <  6:93618/148‑1 (MQ=255)
                                                aTGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAAc                                                                                               >  4:350828/1‑149 (MQ=255)
                                                     cacCGTTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAgg                                                                                          <  7:307431/149‑1 (MQ=255)
                                                     cacCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAgg                                                                                          <  3:141328/149‑1 (MQ=255)
                                                     cacCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAgg                                                                                          >  1:80315/1‑149 (MQ=255)
                                                     cacCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAgg                                                                                          <  2:275439/149‑1 (MQ=255)
                                                     cacCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAgg                                                                                          <  3:15952/149‑1 (MQ=255)
                                                      acCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAggg                                                                                         >  5:209725/1‑149 (MQ=255)
                                                      acCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGACAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAAACGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAggg                                                                                         >  8:314629/1‑149 (MQ=255)
                                                       ccGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGc                                                                                        <  5:164146/149‑1 (MQ=255)
                                                        cGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCt                                                                                                             <  2:106336/128‑1 (MQ=255)
                                                        cGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCt                                                                                                             >  1:106336/1‑128 (MQ=255)
                                                        cGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGCTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCg                                                                                       <  1:18073/149‑1 (MQ=255)
                                                             tCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGaa                                                                                   <  4:313990/148‑1 (MQ=255)
                                                             tCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGaa                                                                                   <  4:114343/148‑1 (MQ=255)
                                                             tCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAg                                                                                  >  5:353507/1‑149 (MQ=255)
                                                               gCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGt                                                                                 >  7:251164/1‑148 (MQ=255)
                                                               gCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTa                                                                                >  8:24812/1‑149 (MQ=255)
                                                                cATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTac                                                                               <  7:29930/149‑1 (MQ=255)
                                                                    cTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTacaac                                                                            <  4:352366/148‑1 (MQ=255)
                                                                       gACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTACAACAAgg                                                                        >  1:209136/1‑149 (MQ=255)
                                                                       gACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTACAACAAgg                                                                        >  5:166589/1‑149 (MQ=255)
                                                                       gACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAATGAAGGAAATCGAGAGCCAGTACGCCCC‑CGGCCTGGTAACCCAGGGCGAGAAGTACAACAAgg                                                                        >  4:208520/1‑149 (MQ=255)
                                                                       gACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTAATACCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGAAAGTACGCATC‑CGGCCTTGTAACCCAGGGCGAGAAGTACAACAAgg                                                                        >  7:178324/1‑149 (MQ=255)
                                                                        aCCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTACAACAAgg                                                                        >  7:44877/1‑148 (MQ=255)
                                                                          cATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCa                                                                     >  5:202203/1‑149 (MQ=255)
                                                                            tttCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATc                                                                   <  6:353507/149‑1 (MQ=255)
                                                                            tttCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATc                                                                   <  5:349014/149‑1 (MQ=255)
                                                                                cGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACt                                                               <  6:166589/149‑1 (MQ=255)
                                                                                       tCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGt                                                         <  5:213661/148‑1 (MQ=255)
                                                                                           tCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAATGAAGGAAATCGAGAGCCAGTACGCCCC‑CGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAg                                                    <  3:208520/149‑1 (MQ=255)
                                                                                                 ttAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAac                                              <  8:295988/149‑1 (MQ=255)
                                                                                                   aaCGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAacga                                            >  8:42746/1‑149 (MQ=255)
                                                                                                       aCTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCTATGAAGTGAAGTAAATCGAGAGCCATTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGaa                                        <  8:178324/149‑1 (MQ=255)
                                                                                                       aCTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGaa                                        <  8:244343/149‑1 (MQ=255)
                                                                                                         ttCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAgt                                      >  6:361124/1‑149 (MQ=255)
                                                                                                         ttCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAgt                                      >  5:1486/1‑149 (MQ=255)
                                                                                                             ttATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTcc                                  <  2:296784/149‑1 (MQ=255)
                                                                                                                  ccGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGc                             >  4:73286/1‑149 (MQ=255)
                                                                                                                   cGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAATTGAAGGAAATCGAGAGCCAGTACGCCTC‑AGGCCTGGTAACCCAGGGCGAGAAGTAGAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGc                             >  8:319024/1‑148 (MQ=255)
                                                                                                                     gACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCAAAGGCgat                          >  2:301115/1‑149 (MQ=255)
                                                                                                                        gagaAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCgatgat                       >  6:157589/1‑149 (MQ=255)
                                                                                                                         agaAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTtgt                                                            >  7:37662/1‑111 (MQ=255)
                                                                                                                         agaAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTtgt                                                            <  8:37662/111‑1 (MQ=255)
                                                                                                                         agaAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGatgatg                      >  3:306486/1‑149 (MQ=255)
                                                                                                                          gaAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATgg                     <  7:24812/149‑1 (MQ=255)
                                                                                                                            aaGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGc                    <  7:93373/148‑1 (MQ=255)
                                                                                                                                 cGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAAcc               >  5:12301/1‑148 (MQ=255)
                                                                                                                                  acttcatcGGTAGCGTTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACctc             >  8:316478/4‑149 (MQ=255)
                                                                                                                                    ttcatcGGTAGCGTTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACCtctc           >  7:38339/2‑149 (MQ=255)
                                                                                                                                        tcGGTAGCGTTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACCTCTCGaaa       <  7:316478/149‑1 (MQ=255)
                                                                                                                                        tcGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGAGGGCCAACCTCTCGaaa       <  2:16493/149‑1 (MQ=255)
                                                                                                                                           gTAACGCTACCTATGAATTGAAGGAAATAGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACCTCTCGAAagag    <  7:118002/149‑1 (MQ=255)
                                                                                                                                           gTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTCTCGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACCTCTCGaaa       >  1:270499/1‑147 (MQ=255)
                                                                                                                                              aCGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACCTCTCGAAAGAGaa  <  8:251164/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
TACCGCGTGGTTGGTCTGAAAGAGACCGTTATCTTCGCCGACCAACTGATGTACACCGGTTTCGCATACTCGACCATTTCCGGCGTTTCCATCGGTGTTAACGACTTCGTTATCCCGGACGAGAAAGCCCGCATCATCGGTAACGCTACCGATGAAGTGAAGGAAATCGAGAGCCAGTACGCCTC‑CGGCCTGGTAACCCAGGGCGAGAAGTACAACAAGGTCATCGACTTGTGGTCGAAGGCGAACGACGAAGTGTCCAAGGCGATGATGGCCAACCTCTCGAAAGAGAA  >  NC_002947/543585‑543874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑