Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 571,214 G→C 6.1% K62N (AAG→AAC)  ssb → single‑stranded DNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947571,2140GC 6.1% 208.5 / 3.8 65K62N (AAG→AAC) ssbsingle‑stranded DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (27/34);  new base C (2/2);  total (29/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.58e-01

GCCAGGATCCCGAAGTCCGCTACCTGCCCAACGGTAACGCCGTGACCAACCTGAGCCTGGCCACCAGCGAGCAGTGGACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGACCCGCGAGTGGGAAAAAGACGGCATCAAGCGCTACACCACCGAGATCATCGTCGATATCAACGGCACCATGCAGCTG  >  NC_002947/571072‑571358
                                                                                                                                              |                                                                                                                                                
gCCAGGATCCCGAAGTCCGCTACCTGCCCAACGGTAACGCCGTGACCAACCTGAGCCTGGCCACCAGCGAGCAGTGGACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGcc                                                                                                                                            >  1:258172/1‑149 (MQ=255)
gCCAGGATCCCGAAGTCCGCTACCTGCCCAACGGTAACGCCGTGACCAACCTGAGCCTGGCCACCAGCGAGCAGTGGACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGc                                                                                                                                             <  4:92704/148‑1 (MQ=255)
    ggATCCCGAAGTCCGCTACCTGCCCAACGGTAACGCCGTGACCAACCTGAGCCTGGCCACCAGCGAGCAGTGGACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGaaa                                                                                                                                        <  5:260762/149‑1 (MQ=255)
         ccGAAGTCCGCTACCTGCCCAACGGTAACGCCGTGACCAACCTGAGCCTGGCCACCAGCGAGCAGTGGACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAACCTTGCCGaacagcga                                                                                                                                   <  5:24636/149‑7 (MQ=255)
          cGAAGTGCGCTACCTGCGCAACCGTATGGCCGTGACCAACGTGAGCCTGGCCACGAGCGAGCTGTGGACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTg                                                                                                                                  <  5:228763/149‑1 (MQ=255)
          cGAAGTCCGCTACCTGCCCAACGGTAACGCCGTGACCAACCTGAGCCTGGCCACCAGCGAGCAGTGGACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGcc                                                                                                                                            <  5:134534/139‑1 (MQ=255)
          cGAAGTCCGCTACCTGCCCAACGGTAACGCCGTGACCAACCTGAGCCTGGCCACCAGCGAGCAGTGGACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGcc                                                                                                                                            >  6:134534/1‑139 (MQ=255)
          cGAAGTCCGCTACCTGCCCAACGGTAACGCCGTGACCAACCTGAGCCTGGCCACCAGCGAGCAGTGGACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTg                                                                                                                                  <  2:13778/149‑1 (MQ=255)
          cGAAGTCCGCTACCTGCCCAACGGTAACGCCGTGACCAACCTGAGCCTGGCCACCAGCGAGCAGTGGACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTg                                                                                                                                  >  5:372911/1‑149 (MQ=255)
           gAAGTCCGCTACCTGCCCAACGGTAACGCCGTGACCAACCTGAGCCTGGCCACCAGCGAGCAGTGGACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTgg                                                                                                                                 >  7:304698/1‑149 (MQ=255)
           gAAGTCCGCTACCTGCCCAACGGTAACGCCGTGACCAACCTGAGCCTGGCCACCAGCGAGCAGTGGACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTgg                                                                                                                                 >  8:241850/1‑149 (MQ=255)
                      ccTGCCCAACGGTAACGCCGTGACCAACCTGAGCCTGGCCACCAGCGAGCAGTGGACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTgc                                                                                                                      <  6:261029/149‑1 (MQ=255)
                      ccTGACCAACGGTAACGACGTGACCAACCTGAGCCTGGCCACCAGCGAGCAGTGGACTGAAAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCAACGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTgc                                                                                                                      <  8:308516/149‑1 (MQ=255)
                       cTGCCCAACGGTAACGCCGTGACCAACCTGAGCCTGGCCACCAGCGAGCAGTGGACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTgcg                                                                                                                     >  1:233147/1‑149 (MQ=255)
                        tGCCCAACGGTAACGCCGTGACCAACCTGAGCCTGGCCACCAGCGAGCAGTGGACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTgcg                                                                                                                     >  1:48627/1‑148 (MQ=255)
                          cccAACGGTAACGCCGTGACCAACCTGAGCCTGGCCACCAGCGAGCAGTGGACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCaa                                                                                                                  <  1:38026/149‑1 (MQ=255)
                          cccAACGGTAACGCCGTGACCAACCTGAGCCTGGCCACCAGCGAGCAGTGGACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCaa                                                                                                                  <  6:178168/149‑1 (MQ=255)
                             aaCGGTAACGCCGTGACCAACCTGAGCCTGGCCACCAGCGAGCAGTGGACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAggg                                                                                                               <  2:236349/149‑1 (MQ=255)
                               cGGTAACGCCGTGACCAACCTGAGCCTGGCCACCAGCGAGCAGTGGACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGtt                                                                                                             >  8:323371/1‑149 (MQ=255)
                                 gTAACGCCGTGACCAACCTGAGCCTGGCCACCAGCGAGCAGTGGACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCg                                                                                                           >  5:157845/1‑149 (MQ=255)
                                   aaCGCCGTGACCAACCTGCGCCTGGCCACCAGCGAGCAGTGGACTGACAAGCAGGCGGGCCAGAAGGTCGAGCGTACCGAGGGGCACCGGGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCgta                                                                                                         <  6:195805/149‑3 (MQ=255)
                                     cGCCGTGACCAACCTGAGCCTGGCCACCAGCGAGCAGTGGACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGt                                                                                                       <  3:95572/149‑1 (MQ=255)
                                       ccGTGACCAACCTGAGCCTGGCCACCAGCGAGCAGTGGACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTTGCGAATACCTGCGCAAGGGTTCGCAGTGc                                                                                                     <  6:312805/149‑1 (MQ=255)
                                             ccAACCTGAGCCTGGCCACCAGCGAGCAGTGGACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATc                                                                                               <  6:131887/149‑1 (MQ=255)
                                                        cTGGCCACCAGCGAGCAGTGGACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCt                                                                                    <  3:151366/149‑1 (MQ=255)
                                                           gCCACCAGCGAGCAGTGGACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTg                                                                                   <  4:367374/147‑1 (MQ=255)
                                                           gCCACCAGCGAGCAGTGGACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGc                                                                                  <  2:258172/148‑1 (MQ=255)
                                                             caccaGCGAGCAGTGGACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTTTTCGGCAACCTTGCCGaaca                                                                                                                                       <  8:111914/93‑3 (MQ=255)
                                                             caccaGCGAGCAGTGGACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAACCTTGCCGaaca                                                                                                                                       >  7:111914/1‑91 (MQ=255)
                                                              accaGCGAGCAGTGGACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGAc                                                                              <  4:41286/149‑1 (MQ=255)
                                                                  gCGAGCAGTGGACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGACCcgc                                                                          >  5:300670/1‑149 (MQ=255)
                                                                    gAGCAGTGGACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGACCCGCGa                                                                        <  3:368153/149‑1 (MQ=255)
                                                                    gAGCAGTGGACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGACCCGCGa                                                                        <  2:233147/149‑1 (MQ=255)
                                                                       cAGTGGACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGACCCGCGAGTg                                                                     <  8:304698/149‑1 (MQ=255)
                                                                            gACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGACCCGCGAGTGGaaa                                                                 >  3:54406/1‑148 (MQ=255)
                                                                            gACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGACCCGCGAGTGGGaaa                                                                <  2:48627/149‑1 (MQ=255)
                                                                            gACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGACCCGCGAGTGGGaa                                                                 >  5:195227/1‑148 (MQ=255)
                                                                              cTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGACCCGCGAGTGGGaaaaa                                                              <  6:372911/149‑1 (MQ=255)
                                                                               tGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGACCCGCGAGTGGGaaa                                                                >  1:310696/1‑146 (MQ=255)
                                                                               tGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGCTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACCTCGAAGGCAAGCTGCAGACCCGCGAGTGGGaaa                                                                >  6:251263/1‑146 (MQ=255)
                                                                                 aCAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGACCCGCGAGTGGGAAAAAGAc                                                           >  2:109969/1‑149 (MQ=255)
                                                                                 aCAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGACCCGCGAGTGGGAAAAAGAc                                                           >  2:105257/1‑149 (MQ=255)
                                                                                   aaGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGACCCGCGAGTGGGAAAAAGACgg                                                         <  6:300670/149‑1 (MQ=255)
                                                                                     gCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGaa                                                                                            >  5:52522/1‑112 (MQ=255)
                                                                                     gCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGaa                                                                                            <  6:52522/112‑1 (MQ=255)
                                                                                               cAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGACCCGCGAGTGGGAAAAAGACGGCATCAAGCGCTa                                             >  5:265721/1‑149 (MQ=255)
                                                                                                aGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGACCCGCGAGTGGGAAAAAGACGGCATCAAGCGCTac                                            >  6:334159/1‑149 (MQ=255)
                                                                                                 gAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGACCCGCGAGTGGGAAAAAGACGGCATCAAGCGCTaca                                           <  2:102171/149‑1 (MQ=255)
                                                                                                      tCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCTCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGACCCGCGAGTGGGAAAAAGACGGCATCAAGCGCTAcaccac                                       <  7:241850/148‑1 (MQ=255)
                                                                                                          gCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGACCCGCGAGTGGGAAAAAGACGGCATCAAGCGCTACACCACCgaga                                  <  5:334159/149‑1 (MQ=255)
                                                                                                               ccGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGACCCGCGAGTGGGAAAAAGACGGCATCAAGCGCTACACCACCGAGatcatc                             <  6:265721/149‑1 (MQ=255)
                                                                                                                    tGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGACCCGCGAGTGGGAAAAAGACGGCATCAAGCGCTACACCACCGAGATCATCGTCGa                        <  1:109969/149‑1 (MQ=255)
                                                                                                                    tGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGACCCGCGAGTGGGAAAAAGACGGCATCAAGCGCTACACCACCGAGATCATCGTCGa                        <  1:105257/149‑1 (MQ=255)
                                                                                                                      gCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGACCCGCGAGTGGGAAAAAGACGGCATCAAGCGCTACACCACCGAGATCAtcgt                           >  4:227285/1‑144 (MQ=255)
                                                                                                                      gCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGACCCGCGAGTGGGAAAAAGACGGCATCAAGCGCTACACCACCGAGATCAtcgt                           <  3:227285/144‑1 (MQ=255)
                                                                                                                       cACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGACCCGCGAGTGGGAAAAAGACGGCATCAAGCGCTACACCACCGAGATCATCGTCGatat                     <  7:323371/149‑1 (MQ=255)
                                                                                                                       cACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGACCCGCGAGTGGGAAAAAGACGGCATCAAGCGCTACACCACCGAGATCATCGTCGata                      >  3:27246/1‑148 (MQ=255)
                                                                                                                        aCCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGACCCGCGAGTGGGAAAAAGACGGCATCAAGCGCTa                                             >  6:315121/1‑124 (MQ=255)
                                                                                                                        aCCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGACCCGCGAGTGGGAAAAAGACGGCATCAAGCGCTa                                             <  5:315121/124‑1 (MQ=255)
                                                                                                                        aCCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGACCCGCGAGTGGGAAAAAGACGGCATCAAGCGCTACACCACCGAGATCATCGTCGatat                     >  2:205810/1‑148 (MQ=255)
                                                                                                                                tCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGACCCGCGAGTGGGAAAAAGACGGCATCAAGCGCTACACCACCGAGATCATCGTCGATATCAACGGCAc            >  4:90430/1‑149 (MQ=255)
                                                                                                                                  gCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGACCCGCGAGTGGGAAAAAGACGGCATCAAGCGCTACACCACCGAGATCATCGTCGATATCAACGGCACCa          >  1:223009/1‑149 (MQ=255)
                                                                                                                                    atttCGGCAACCTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGACCCGCGAGTGGGAAAAAGACGGCATCAAGCGCTACACCACCGAGATCATCGTCGATATCAACGGCACCATg        >  2:367626/3‑149 (MQ=255)
                                                                                                                                       tCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGACCCGCGAGTGGGAAAAAGACGGCATCAAGCGCTACACCACCGAGATCATCGTCGATATCAACGGCACCATGCAg     >  8:89990/1‑149 (MQ=255)
                                                                                                                                          gCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGACCCGCGAGTGGGAAAAAGACGGCATCAAGCGCTACACCACCGAGATCATCGTCGATATCAACGGCACCATGCAgctg  >  2:73715/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                
GCCAGGATCCCGAAGTCCGCTACCTGCCCAACGGTAACGCCGTGACCAACCTGAGCCTGGCCACCAGCGAGCAGTGGACTGACAAGCAGTCGGGCCAGAAGGTCGAGCGTACCGAGTGGCACCGTGTGTCGCTGTTCGGCAAGGTTGCCGAAATCGCTGGCGAATACCTGCGCAAGGGTTCGCAGTGCTACATCGAAGGCAAGCTGCAGACCCGCGAGTGGGAAAAAGACGGCATCAAGCGCTACACCACCGAGATCATCGTCGATATCAACGGCACCATGCAGCTG  >  NC_002947/571072‑571358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑