Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 572,518 A→G 6.2% I191T (ATC→ACC)  PP_0486 ← GntR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947572,5180AG 6.2% 188.3 / 2.5 64I191T (ATC→ACC) PP_0486GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (31/29);  new base G (2/2);  total (33/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

GCGCGCAATGCCTCCGGGATGAGCCCGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGACCACCCTCTGTCACATAGTCGAGTACCTGCCCC  >  NC_002947/572374‑572664
                                                                                                                                                |                                                                                                                                                  
gcgcgcAATGCCTCCGGGATGAGCCCGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGc                                                                                                                                                <  5:58284/149‑1 (MQ=255)
    gcAATGCCTCCGGGATGAGCCCGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGccacc                                                                                                                                            <  1:304575/149‑1 (MQ=255)
     cAATGCCTCCGGGATGAGCCCGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGccacca                                                                                                                                           <  3:319008/149‑1 (MQ=255)
          ccTCCGGGATGAGCCCGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGGTGGCCATCagcagc                                                                                                                                      >  7:294359/1‑149 (MQ=255)
           cTCCGGGATGAGCCCGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGcc                                                                                                                                     >  8:172902/1‑149 (MQ=255)
              cGGGATGAGCCCGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGGTGGCCATCAGCAGcct                                                                                                                                    <  7:232963/147‑2 (MQ=255)
              cGGGATGAGCCCGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGGTGGCCATCAGCAGcct                                                                                                                                    <  8:294359/147‑2 (MQ=255)
                     aGCCCGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGgc                                                                                                                           <  1:62899/149‑1 (MQ=255)
                      gCCCGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGgcg                                                                                                                          <  6:272843/149‑1 (MQ=255)
                       cccGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCagcagc                                                                                                                                      <  7:285533/136‑1 (MQ=255)
                       cccGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCagcagc                                                                                                                                      >  8:285533/1‑136 (MQ=255)
                       cccGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGgcgc                                                                                                                         >  4:274422/1‑149 (MQ=255)
                         cGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCa                                                                                                                       <  7:344265/149‑1 (MQ=255)
                                       gCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGtacta                                                                                                          <  3:121500/148‑1 (MQ=255)
                                       gCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGtacta                                                                                                          <  5:230549/148‑1 (MQ=255)
                                            gccgcGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACctgct                                                                                                    <  7:172902/149‑1 (MQ=255)
                                              cgcgCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCtgctgc                                                                                                  <  6:45724/149‑1 (MQ=255)
                                                            cccAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAgg                                                                                    <  2:128930/149‑1 (MQ=255)
                                                             ccAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAgg                                                                                    >  6:69393/1‑148 (MQ=255)
                                                              cAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGca                                                                                  <  3:274422/149‑1 (MQ=255)
                                                               aGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGcag                                                                                 <  7:327966/149‑1 (MQ=255)
                                                                ggCTGTGGGCCAGGTATAGGATGCCCGGGTAGCCGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGcagc                                                                                <  8:291212/149‑1 (MQ=255)
                                                                  gcctgGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGcagcca                                                                              <  5:185589/146‑1 (MQ=255)
                                                                     tgGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAgcg                                                                           >  2:314185/1‑149 (MQ=255)
                                                                       ggCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACGTGCTGCCAGCGAGCATCAGGCAGCCAgcgc                                                                          <  1:32560/148‑1 (MQ=255)
                                                                       ggCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCt                                                                         <  5:30927/149‑1 (MQ=255)
                                                                       ggCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCt                                                                         >  6:30927/1‑149 (MQ=255)
                                                                        gCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTg                                                                        >  8:117732/1‑149 (MQ=255)
                                                                          cAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTa                                                                      >  8:258713/1‑149 (MQ=255)
                                                                           aGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTAt                                                                     >  2:6644/1‑149 (MQ=255)
                                                                               atatGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAg                                                                 <  8:200632/149‑1 (MQ=255)
                                                                                      gCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCAc                                                          >  8:299691/1‑149 (MQ=255)
                                                                                       cccGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGc                                                                                          >  5:160320/1‑116 (MQ=255)
                                                                                       cccGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGc                                                                                          <  6:160320/116‑1 (MQ=255)
                                                                                                 agagCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCttgtttg                                               >  4:217000/1‑149 (MQ=255)
                                                                                                  gagCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCttgtttgt                                              >  4:52597/1‑149 (MQ=255)
                                                                                                  gagCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCttgtttgt                                              <  6:324141/149‑1 (MQ=255)
                                                                                                   agCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGCGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTtgtg                                             >  4:87062/1‑149 (MQ=255)
                                                                                                           gCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACgtgt                                     >  3:361275/1‑149 (MQ=255)
                                                                                                            cacaGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGtgtg                                    <  7:299691/149‑1 (MQ=255)
                                                                                                              caGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGa                                   <  2:57592/148‑1 (MQ=255)
                                                                                                              caGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGa                                   >  1:57592/1‑148 (MQ=255)
                                                                                                                gCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGacca                                <  4:361275/149‑1 (MQ=255)
                                                                                                                 caccacATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGaccac                               >  3:90488/1‑149 (MQ=255)
                                                                                                                    cacaTCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCttgtttgt                                              >  7:311204/1‑131 (MQ=255)
                                                                                                                    cacaTCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCttgtttgt                                              <  8:311204/131‑1 (MQ=255)
                                                                                                                           tCACTCTTCGACAGGCTGCTGATCGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCACGTCGCACCGGCTTGTTTGTGGGACGTGTGACCACCCTCTGTca                      >  7:211753/1‑148 (MQ=255)
                                                                                                                             aCTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGACCACCCTCTGTcaca                    >  5:52864/1‑148 (MQ=255)
                                                                                                                              ctctTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCtt                                                    <  3:335578/115‑1 (MQ=255)
                                                                                                                              ctctTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCtt                                                    >  4:335578/1‑115 (MQ=255)
                                                                                                                               tctTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGACCACCCTCTGTCACATAg                 >  7:200268/1‑149 (MQ=255)
                                                                                                                                ctTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGACCACCCTCTGTCACATAGt                >  2:59721/1‑149 (MQ=255)
                                                                                                                                 ttCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGACCACCCTCTGTCACATAGTc               >  6:278715/1‑149 (MQ=255)
                                                                                                                                     aCAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGACCACCCTCTGTCACATAGTCGa             >  2:30800/1‑147 (MQ=255)
                                                                                                                                     aCAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGCACGTGTGACCACCCTCTGTCACATAGTCGa             <  1:30800/147‑1 (MQ=255)
                                                                                                                                          ctgctgGTGGCCATCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGACCACCCTCTGTCACATAGTCGAGTACCTg      >  3:350709/1‑149 (MQ=255)
                                                                                                                                           tgctgATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGACCACCCTCTGTCACATAGTCGAGTACCTGc     >  7:303322/1‑149 (MQ=255)
                                                                                                                                            gctgATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTAATACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGACCACCCTCTGTCACATAGTCGAGTACCTGcc    >  5:233602/1‑149 (MQ=255)
                                                                                                                                             ctgATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACgtgt                                     >  6:76931/1‑115 (MQ=255)
                                                                                                                                             ctgATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACgtgt                                     <  5:76931/115‑1 (MQ=255)
                                                                                                                                               gATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTtgtg                                             >  6:39824/1‑105 (MQ=255)
                                                                                                                                               gATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTtgtg                                             <  5:39824/105‑1 (MQ=255)
                                                                                                                                               gATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGACCACCCTCTGTCACATAGTCGAGTACCTGcccc  >  7:335567/1‑148 (MQ=255)
                                                                                                                                               gATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGACCACCCTCTGTCACATAGTCGAGTACCTGcccc  >  7:320952/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
GCGCGCAATGCCTCCGGGATGAGCCCGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGACCACCCTCTGTCACATAGTCGAGTACCTGCCCC  >  NC_002947/572374‑572664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑