Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 676,102 T→C 8.5% Q103Q (CAA→CAG)  PP_0578 ← putative Methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947676,1020TC 8.5% 163.0 / 5.1 59Q103Q (CAA→CAG) PP_0578putative Methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (27/27);  new base C (3/2);  total (30/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01

TAGACCAGGGCGTCGCGGTTGTAGAAGGCCAGCGATAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGTTCGGCCAGGGTCAGCTGGTGGCCGCGCTCGGCCAGCCACAAGGC  >  NC_002947/675956‑676245
                                                                                                                                                  |                                                                                                                                               
tAGACCAGGGCGTCGCGGTTGTAGAAGGCCAGCGATAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTg                                                                                                                                               <  3:201290/149‑1 (MQ=255)
    ccAGGGCGTCGCGGTTGTAGAAGGCCAGCGATAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCg                                                                                                                                           <  2:368635/149‑1 (MQ=255)
       gggCGTCGCGGTTGTAGAAGGCCAGCGATAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCgagg                                                                                                                                        <  3:268901/149‑1 (MQ=255)
       gggCGTCGCGGTTGTAGAAGGCCAGCGATAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGCCAACTGACCgagc                                                                                                                                        <  5:57689/149‑2 (MQ=255)
              gcgGTTGTAGAAGGCCAGCGATAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCtt                                                                                                                                 <  4:114610/149‑1 (MQ=255)
                  ttGTAGAAGGCCAGCGATAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGc                                                                                                                               >  4:273933/1‑147 (MQ=255)
                    gTAGAAGGACAGCGATAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAg                                                                                                                            <  8:154776/148‑1 (MQ=255)
                         aGGCCAGCGATAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGcc                                                                                                                      <  1:150120/149‑1 (MQ=255)
                          ggCCAGCGATAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCt                                                                                                                     <  3:140320/149‑1 (MQ=255)
                            ccAGCGATAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGCCAACTGACCgagc                                                                                                                                        <  1:110546/128‑2 (MQ=255)
                            ccAGCGATAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGCCAACTGACCgagc                                                                                                                                        >  2:110546/1‑127 (MQ=255)
                             cAGCGATAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCATTTGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGa                                                                                                                  <  1:311547/149‑1 (MQ=255)
                             cAGCGATAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGGGCCTGGa                                                                                                                  >  8:95951/1‑149 (MQ=255)
                             cAGCGATAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGa                                                                                                                  >  7:1587/1‑149 (MQ=255)
                                  aTAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGaaa                                                                                                             <  1:197500/149‑1 (MQ=255)
                                  aTAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGaaa                                                                                                             <  1:244755/149‑1 (MQ=255)
                                       caaccGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTcgc                                                                                                        >  7:286959/1‑149 (MQ=255)
                                        aaccGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTcgcg                                                                                                       >  4:178423/1‑149 (MQ=255)
                                        aaccGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTcgcg                                                                                                       <  3:359395/149‑1 (MQ=255)
                                         accGCCGGGGGCCGTGAGCTGGCGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCgg                                                                                                      >  2:209186/1‑149 (MQ=255)
                                          ccgccgGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCgga                                                                                                     >  1:319441/1‑148 (MQ=255)
                                                     cGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCtt                                                                                          >  2:358549/1‑149 (MQ=255)
                                                     cGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCtg                                                                                          >  4:281519/1‑148 (MQ=255)
                                                      gTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTc                                                                                         <  6:5981/149‑1 (MQ=255)
                                                         aGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCgg                                                                                       <  7:112020/148‑1 (MQ=255)
                                                           cTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCAGCAGGTCGTAGCGCTCGGTCAGTTGGCCGCGGAGGTCTTGCCAGGGCGCCTGGACGAAAGTCGCGGTCTGGCCGGCTTCGGCaa                                                                                    >  7:336854/1‑149 (MQ=255)
                                                              gTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCaaa                                                                                   <  8:198488/147‑1 (MQ=255)
                                                              gTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCaaa                                                                                   <  1:156392/147‑1 (MQ=255)
                                                              gTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGc                                                                                 <  2:100452/149‑1 (MQ=255)
                                                                   gcaCCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGcc                                                                            >  3:125531/1‑149 (MQ=255)
                                                                    caCCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGccc                                                                           >  7:217130/1‑149 (MQ=255)
                                                                     aCCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCt                                                                          >  6:209239/1‑149 (MQ=255)
                                                                             cctGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACGAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGTGCCGt                                                                  >  3:291492/3‑149 (MQ=255)
                                                                              aTGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTc                                                                 <  8:217130/149‑1 (MQ=255)
                                                                               tGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTc                                                                 >  5:1664/1‑148 (MQ=255)
                                                                               tGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTc                                                                 <  6:1664/148‑1 (MQ=255)
                                                                                  tctcGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCGGAGGAGGTCTTGCCAGGGCGCCTGGAGGAAAGTCGCGGTCTGGCCGGATTCGGCAACGCGTGCCCTGGCGCCGTCGAGc                                                             >  5:206296/1‑149 (MQ=255)
                                                                                      gggTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCAt                                                           <  4:125531/147‑1 (MQ=255)
                                                                                      gggTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATg                                                          <  3:178423/148‑1 (MQ=255)
                                                                                            ggCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGcgccg                                                   >  4:301646/1‑149 (MQ=255)
                                                                                               cagccaTTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAg                                                <  3:31046/149‑1 (MQ=255)
                                                                                                 gccaTTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGt                                              <  1:358549/149‑1 (MQ=255)
                                                                                                        gAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGTTTCGGc                                         >  7:950/1‑147 (MQ=255)
                                                                                                         aGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGTTCGGCCAg                                      <  8:1587/149‑1 (MQ=255)
                                                                                                          gCACGGCGTGGCAGAGAACCACCAACAACCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCATGGATGAAAGTCGCGGTCTGGCCGGCATCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGTTCGGCCAgg                                     >  6:175805/1‑149 (MQ=255)
                                                                                                               gCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGTTCGGCCAGGGTCAg                                >  4:112748/1‑149 (MQ=255)
                                                                                                                  tGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGCGCGCCGCAGGTTCGGCCAGGGTCAGCtg                             <  1:209186/149‑1 (MQ=255)
                                                                                                                    gCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGTTCGGCCAGGGTCAGCtggt                           >  3:354507/1‑149 (MQ=255)
                                                                                                                    gCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGTTCGGCCAGGGTCAGCtggt                           >  1:182551/1‑149 (MQ=255)
                                                                                                                      agagCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGTTCGGCCAGGGTCAGCtggtgg                         >  7:59440/1‑149 (MQ=255)
                                                                                                                         gCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGTTCGGCCAGGGTCAGCTGGTGGCcg                      >  5:263677/1‑149 (MQ=255)
                                                                                                                             cAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGTTCGGCCAGGGTCAGCTGGTGGCCGCGCt                  >  6:77196/1‑149 (MQ=255)
                                                                                                                                      ctccTCGGCCAACTGACCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCT‑GCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGTTCGGCCAGGGTCAGCTGGTGGCCGCGCTCGgccagcca        >  2:314739/4‑149 (MQ=255)
                                                                                                                                       tccTCGGCCAACTGACCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGTTCGGCCAGGGTCAGCTGGTGGCCGCGCTCGgccagcca        >  5:129711/3‑149 (MQ=255)
                                                                                                                                           cGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGTTCGGCCAGGGTCAGCTGGTGGCCGCGCTCGGCCAGCCACAAg    <  6:263677/149‑1 (MQ=255)
                                                                                                                                           cGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGTTCGGCCAGGGTCAGCTGGTGGCCGCGCTCGGCCAGCCACAAg    <  3:112748/149‑1 (MQ=255)
                                                                                                                                             gTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGTTCGGCCAgg                                     <  2:252230/114‑1 (MQ=255)
                                                                                                                                             gTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGTTCGGCCAgg                                     >  1:252230/1‑114 (MQ=255)
                                                                                                                                             gTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGTTCGGCCAGGGTCAGCTGGTGGCCGCGCTCGGCCAGCCACAAGGc  >  5:311482/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
TAGACCAGGGCGTCGCGGTTGTAGAAGGCCAGCGATAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGTTCGGCCAGGGTCAGCTGGTGGCCGCGCTCGGCCAGCCACAAGGC  >  NC_002947/675956‑676245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑