Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 676,105 G→A 8.6% G102G (GGC→GGT)  PP_0578 ← putative Methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947676,1050GA 8.6% 162.5 / 4.9 58G102G (GGC→GGT) PP_0578putative Methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (27/26);  new base A (3/2);  total (30/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

CCAGGGCGTCGCGGTTGTAGAAGGCCAGCGATAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGTTCGGCCAGGGTCAGCTGGTGGCCGCGCTCGGCCAGCCACAAGGC  >  NC_002947/675960‑676245
                                                                                                                                                 |                                                                                                                                            
ccAGGGCGTCGCGGTTGTAGAAGGCCAGCGATAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCg                                                                                                                                           <  2:368635/149‑1 (MQ=255)
   gggCGTCGCGGTTGTAGAAGGCCAGCGATAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCgagg                                                                                                                                        <  3:268901/149‑1 (MQ=255)
   gggCGTCGCGGTTGTAGAAGGCCAGCGATAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGCCAACTGACCgagc                                                                                                                                        <  5:57689/149‑2 (MQ=255)
          gcgGTTGTAGAAGGCCAGCGATAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCtt                                                                                                                                 <  4:114610/149‑1 (MQ=255)
              ttGTAGAAGGCCAGCGATAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGc                                                                                                                               >  4:273933/1‑147 (MQ=255)
                gTAGAAGGACAGCGATAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAg                                                                                                                            <  8:154776/148‑1 (MQ=255)
                     aGGCCAGCGATAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGcc                                                                                                                      <  1:150120/149‑1 (MQ=255)
                      ggCCAGCGATAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCt                                                                                                                     <  3:140320/149‑1 (MQ=255)
                        ccAGCGATAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGCCAACTGACCgagc                                                                                                                                        <  1:110546/128‑2 (MQ=255)
                        ccAGCGATAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGCCAACTGACCgagc                                                                                                                                        >  2:110546/1‑127 (MQ=255)
                         cAGCGATAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCATTTGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGa                                                                                                                  <  1:311547/149‑1 (MQ=255)
                         cAGCGATAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGGGCCTGGa                                                                                                                  >  8:95951/1‑149 (MQ=255)
                         cAGCGATAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGa                                                                                                                  >  7:1587/1‑149 (MQ=255)
                              aTAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGaaa                                                                                                             <  1:197500/149‑1 (MQ=255)
                              aTAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGaaa                                                                                                             <  1:244755/149‑1 (MQ=255)
                                   caaccGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTcgc                                                                                                        >  7:286959/1‑149 (MQ=255)
                                    aaccGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTcgcg                                                                                                       >  4:178423/1‑149 (MQ=255)
                                    aaccGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTcgcg                                                                                                       <  3:359395/149‑1 (MQ=255)
                                     accGCCGGGGGCCGTGAGCTGGCGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCgg                                                                                                      >  2:209186/1‑149 (MQ=255)
                                      ccgccgGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCgga                                                                                                     >  1:319441/1‑148 (MQ=255)
                                                 cGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCtt                                                                                          >  2:358549/1‑149 (MQ=255)
                                                 cGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCtg                                                                                          >  4:281519/1‑148 (MQ=255)
                                                  gTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTc                                                                                         <  6:5981/149‑1 (MQ=255)
                                                     aGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCgg                                                                                       <  7:112020/148‑1 (MQ=255)
                                                       cTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCAGCAGGTCGTAGCGCTCGGTCAGTTGGCCGCGGAGGTCTTGCCAGGGCGCCTGGACGAAAGTCGCGGTCTGGCCGGCTTCGGCaa                                                                                    >  7:336854/1‑149 (MQ=255)
                                                          gTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCaaa                                                                                   <  1:156392/147‑1 (MQ=255)
                                                          gTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCaaa                                                                                   <  8:198488/147‑1 (MQ=255)
                                                          gTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGc                                                                                 <  2:100452/149‑1 (MQ=255)
                                                               gcaCCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGcc                                                                            >  3:125531/1‑149 (MQ=255)
                                                                caCCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGccc                                                                           >  7:217130/1‑149 (MQ=255)
                                                                 aCCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCt                                                                          >  6:209239/1‑149 (MQ=255)
                                                                         cctGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACGAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGTGCCGt                                                                  >  3:291492/3‑149 (MQ=255)
                                                                          aTGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTc                                                                 <  8:217130/149‑1 (MQ=255)
                                                                           tGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTc                                                                 >  5:1664/1‑148 (MQ=255)
                                                                           tGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTc                                                                 <  6:1664/148‑1 (MQ=255)
                                                                              tctcGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCGGAGGAGGTCTTGCCAGGGCGCCTGGAGGAAAGTCGCGGTCTGGCCGGATTCGGCAACGCGTGCCCTGGCGCCGTCGAGc                                                             >  5:206296/1‑149 (MQ=255)
                                                                                  gggTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCAt                                                           <  4:125531/147‑1 (MQ=255)
                                                                                  gggTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATg                                                          <  3:178423/148‑1 (MQ=255)
                                                                                        ggCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGcgccg                                                   >  4:301646/1‑149 (MQ=255)
                                                                                           cagccaTTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAg                                                <  3:31046/149‑1 (MQ=255)
                                                                                             gccaTTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGt                                              <  1:358549/149‑1 (MQ=255)
                                                                                                    gAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGTTTCGGc                                         >  7:950/1‑147 (MQ=255)
                                                                                                     aGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGTTCGGCCAg                                      <  8:1587/149‑1 (MQ=255)
                                                                                                      gCACGGCGTGGCAGAGAACCACCAACAACCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCATGGATGAAAGTCGCGGTCTGGCCGGCATCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGTTCGGCCAgg                                     >  6:175805/1‑149 (MQ=255)
                                                                                                           gCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGTTCGGCCAGGGTCAg                                >  4:112748/1‑149 (MQ=255)
                                                                                                              tGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGCGCGCCGCAGGTTCGGCCAGGGTCAGCtg                             <  1:209186/149‑1 (MQ=255)
                                                                                                                gCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGTTCGGCCAGGGTCAGCtggt                           >  3:354507/1‑149 (MQ=255)
                                                                                                                gCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGTTCGGCCAGGGTCAGCtggt                           >  1:182551/1‑149 (MQ=255)
                                                                                                                  agagCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGTTCGGCCAGGGTCAGCtggtgg                         >  7:59440/1‑149 (MQ=255)
                                                                                                                     gCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGTTCGGCCAGGGTCAGCTGGTGGCcg                      >  5:263677/1‑149 (MQ=255)
                                                                                                                         cAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGTTCGGCCAGGGTCAGCTGGTGGCCGCGCt                  >  6:77196/1‑149 (MQ=255)
                                                                                                                                  ctccTCGGCCAACTGACCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCT‑GCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGTTCGGCCAGGGTCAGCTGGTGGCCGCGCTCGgccagcca        >  2:314739/4‑149 (MQ=255)
                                                                                                                                   tccTCGGCCAACTGACCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGTTCGGCCAGGGTCAGCTGGTGGCCGCGCTCGgccagcca        >  5:129711/3‑149 (MQ=255)
                                                                                                                                       cGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGTTCGGCCAGGGTCAGCTGGTGGCCGCGCTCGGCCAGCCACAAg    <  3:112748/149‑1 (MQ=255)
                                                                                                                                       cGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGTTCGGCCAGGGTCAGCTGGTGGCCGCGCTCGGCCAGCCACAAg    <  6:263677/149‑1 (MQ=255)
                                                                                                                                         gTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGTTCGGCCAgg                                     <  2:252230/114‑1 (MQ=255)
                                                                                                                                         gTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGTTCGGCCAgg                                     >  1:252230/1‑114 (MQ=255)
                                                                                                                                         gTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGTTCGGCCAGGGTCAGCTGGTGGCCGCGCTCGGCCAGCCACAAGGc  >  5:311482/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                            
CCAGGGCGTCGCGGTTGTAGAAGGCCAGCGATAACCAACCGCCGGGGGCCGTGAGCTGGTGCAGCACCGGCAGGATGCTCTCGGGTTCGGCCAGCCATTCGAGCACGGCGTGGCAGAGCACCAGGTCGTAGCGCTCGGTCAGTTGGCCGAGGAGGTCTTGCCAGGGCGCCTGGATGAAAGTCGCGGTCTGGCCGGCTTCGGCAAAGCGTGCCCTGGCGCCGTCGAGCATGGGCGCCGCAGGTTCGGCCAGGGTCAGCTGGTGGCCGCGCTCGGCCAGCCACAAGGC  >  NC_002947/675960‑676245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑