| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 697,490 | T→C | 5.8% | intergenic (+417/‑334) | mmsA‑I → / → PP_16SD | methylmalonate‑semialdehyde dehydrogenase/16S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 697,490 | 0 | T | C | 5.8% | 251.6 / 4.3 | 86 | intergenic (+417/‑334) | mmsA‑I/PP_16SD | methylmalonate‑semialdehyde dehydrogenase/16S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base T (43/38); new base C (2/3); total (45/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01 | |||||||||||
GAATCCCCTTATAATGCGCCCCACTTCCAGCGACAACGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGACGAAAAG > NC_002947/697342‑697634 | gAATCCCCTTATAATGCGCCCCACTTCCAGCGACAACGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGt > 5:114794/1‑149 (MQ=255) ccTTATAATGCGCCCCACTTCCAGCGACAACGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCACTTGGAAGC‑GCTGt > 2:52725/1‑148 (MQ=255) cTTATAATGCGCCCCACTTCCAGCGACAACGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTAt > 7:311347/1‑149 (MQ=255) cTTATAATGCGCCCCACTTCCAGCGACAACGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTAt > 4:354044/1‑149 (MQ=255) ataATGCGCCCCACTTCCAGCGACAACGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCACTTGGAAGC‑GCTGtcaa < 6:196405/147‑4 (MQ=25) ataATGCGCCCCACTTCCAGCGACAACGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCACTTGGAAGC‑GCTGtcaa < 1:52725/147‑4 (MQ=25) aaaaTGCGCCCCAATTCCAGCGACAACGGAACTACAAACTCCTTTAAATTAAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCACTTGGAAGC‑GCTGtcaa < 7:281860/145‑4 (MQ=25) tccgcCCCACATCCAGCGACACCGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGc < 4:110362/147‑1 (MQ=255) tGCGCCCCACTTCCAGCGACAACGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑Gc < 3:342376/137‑1 (MQ=255) tGCGCCCCACTTCCAGCGACAACGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTCTAAGGCCTAAGATCAAATGATCGCCAGCCGATGAGATGATGGTTGACATCGCTTACAAGT‑Gc > 4:342376/1‑137 (MQ=25) gcCCCACTTCCAGCGACAACGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTc < 2:284502/149‑1 (MQ=255) cccACTTCCAGCGACAACGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTccc > 4:190692/1‑149 (MQ=255) ttCCAGCGACAACGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTAc > 6:232346/1‑149 (MQ=255) cAGCGACAACGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAGATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACga < 5:300671/148‑1 (MQ=255) cAGCGACAACGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACga < 8:311347/148‑1 (MQ=255) aGCGACAACGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACgaga < 2:192218/149‑1 (MQ=255) cAACGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCg > 4:332795/1‑149 (MQ=255) aaCGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAAATCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGAAGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCg > 1:332817/1‑148 (MQ=255) tgtctaaCTCATTGAATTTCAAGGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGGTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGgagcgagc > 4:203707/6‑149 (MQ=18) cTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGc > 5:86154/1‑112 (MQ=255) cTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGc < 6:86154/112‑1 (MQ=255) cTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTAc > 4:142564/1‑122 (MQ=255) cTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGttt > 3:69985/1‑148 (MQ=255) cTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGttt > 5:245308/1‑148 (MQ=255) cTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGttt > 8:301579/1‑148 (MQ=255) cTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGttt > 1:150846/1‑148 (MQ=255) cTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTg < 3:175101/149‑1 (MQ=255) cTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGATTCCAAGT‑GCTGTATGATTCGCCTCCAGCTACGAGAGATCGCAGCTAGCCAAGTGttt > 8:256478/1‑148 (MQ=255) cTTGAAATTAAAAGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCAAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGttt > 4:177550/1‑148 (MQ=255) ttGAAATTCAATGAGTTAGACAATTCTGAACGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑CCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTg > 8:80533/1‑148 (MQ=255) gAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGa < 2:274565/147‑1 (MQ=255) cAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGTTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTaaa < 4:69985/148‑1 (MQ=255) aaTGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAAc > 6:10281/1‑148 (MQ=255) aaTGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAAc < 5:100709/148‑1 (MQ=255) ttAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTtc < 1:150405/149‑1 (MQ=255) gACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTc > 7:10932/1‑91 (MQ=255) gACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTc < 8:10932/91‑1 (MQ=255) gACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCg < 6:114794/149‑1 (MQ=255) aCAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCg > 4:282188/1‑148 (MQ=255) aaTTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGaa < 2:304816/129‑1 (MQ=255) aaTTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCa > 5:164992/1‑148 (MQ=255) aaTTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCCCTACGAGAGATCGCAGCGAGCCAAGTGTTTGaa > 1:304816/1‑129 (MQ=255) ttCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCaaa > 8:116582/1‑148 (MQ=255) ttCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCaaa > 7:76961/1‑148 (MQ=255) ggTGCTGTAAGGGCTACGATCAAATGATAGCCAGCGGTCGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCa < 7:114957/149‑1 (MQ=255) tGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGTAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCaaa < 1:278915/149‑1 (MQ=255) cTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCAGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCaaaa > 5:279782/1‑148 (MQ=255) tGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCACCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAAt > 3:240085/1‑148 (MQ=255) aGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCTCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAAc < 1:12376/148‑1 (MQ=255) aGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACg > 2:248025/1‑149 (MQ=39) gggCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGTGGGTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAAc > 1:293598/1‑148 (MQ=255) gggCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGc < 7:116582/149‑1 (MQ=39) gggCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGc > 4:287928/1‑149 (MQ=39) gCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCtt > 6:253670/1‑149 (MQ=39) cGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGa < 1:248025/147‑1 (MQ=39) aTCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAgg > 3:283422/1‑149 (MQ=39) aaTGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGctct > 3:126766/1‑149 (MQ=37) aTGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGctct > 8:275622/1‑148 (MQ=37) aTGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTg < 6:245308/149‑1 (MQ=37) tGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCATCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGa < 7:256478/149‑1 (MQ=37) tGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGa < 3:282188/149‑1 (MQ=37) tGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGa < 3:190692/149‑1 (MQ=37) aTCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAg < 7:80533/148‑1 (MQ=37) tCGCCAGCGGTTGAGATGATGCTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCTCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAgg < 2:246067/148‑1 (MQ=25) ccAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAg > 1:78964/1‑149 (MQ=33) ccAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAg > 3:120173/1‑149 (MQ=33) ccAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAg > 3:120219/1‑149 (MQ=33) cAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATaa < 2:301915/124‑1 (MQ=33) cAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATaa > 1:301915/1‑124 (MQ=33) cAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCAGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGc < 6:279782/149‑1 (MQ=17) aGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCg < 1:371317/149‑1 (MQ=32) cGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTa < 7:301579/149‑1 (MQ=32) cGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTa < 3:177550/149‑1 (MQ=32) gatgatGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATgcg > 6:208920/1‑149 (MQ=32) atgatgGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATgcgc < 3:187582/149‑1 (MQ=32) atgatgGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATgcgc < 3:354044/149‑1 (MQ=32) ataCAGCACTTGGAAGC‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGt > 5:335190/3‑149 (MQ=255) gACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAACCTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGtt > 2:235839/1‑149 (MQ=17) aCAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGtt < 5:232346/148‑1 (MQ=32) aCAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGtt < 2:78964/148‑1 (MQ=32) cAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGtt > 6:362906/1‑147 (MQ=32) aGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCt > 2:208951/1‑106 (MQ=32) aGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGtt > 3:118982/1‑146 (MQ=32) aGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTATCTCGCAAAAAACTTCAAAATAAACGCt < 1:208951/106‑1 (MQ=17) gcTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGACAGCGTAGAATGCGCGCCTCGGTTGAGAc > 2:359506/1‑148 (MQ=17) ccAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAACCTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGACGaaa < 3:203707/148‑1 (MQ=17) cAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGACGAAAAg < 4:120219/149‑1 (MQ=32) | GAATCCCCTTATAATGCGCCCCACTTCCAGCGACAACGGAACGACAAACTCCTTGAAATTCAATGAGTTAGACAATTCTGAAGGTGCTGTAAGGGCTACGATCAAATGATCGCCAGCGGTTGAGATGATGGTTGACAGCGCTTCCAAGT‑GCTGTATGATTCGCCTCCCGCTACGAGAGATCGCAGCGAGCCAAGTGTTTGAAGCTAAACGAGTTTCTCGCAAAAAACTTCAAAATAAACGCTTGACAGGCTCTGAGGAAAGCGTAGAATGCGCGCCTCGGTTGAGACGAAAAG > NC_002947/697342‑697634 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |