Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 699,722 A→G 43.7% intergenic (+115/‑129) PP_t06 → / → PP_23SD tRNA‑Ala/23S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947699,7220AG43.7% 46.3 / 122.2 103intergenic (+115/‑129)PP_t06/PP_23SDtRNA‑Ala/23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (29/29);  new base G (22/23);  total (51/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

GAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTT  >  NC_002947/699574‑699803
                                                                                                                                                         |                                                                                 
gaggTCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGa                                                                                   <  3:293591/149‑1 (MQ=37)
gaggTCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGa                                                                                   <  2:117282/149‑1 (MQ=37)
 aggTCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGAc                                                                                  <  7:346151/149‑1 (MQ=37)
  ggTCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCt                                                                                 <  4:38899/149‑1 (MQ=37)
   gTCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTg                                                                                <  2:116583/149‑1 (MQ=37)
   gTCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACt                                                                                 >  8:296419/1‑148 (MQ=37)
    tCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTg                                                                                >  6:161812/1‑148 (MQ=37)
    tCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTg                                                                                >  6:221462/1‑148 (MQ=37)
      aGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGAAATGTGATAGATATAGACTGaa                                                                              >  8:262044/1‑148 (MQ=37)
        cGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAacac                                                                           <  3:289858/149‑1 (MQ=37)
        cGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGa                                                                               >  3:215889/1‑145 (MQ=37)
        cGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGa                                                                               <  4:215889/145‑1 (MQ=37)
        cGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAacac                                                                           <  1:219491/149‑1 (MQ=37)
          gTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCa                                                                         <  4:363249/149‑1 (MQ=37)
          gTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCa                                                                         <  4:139425/149‑1 (MQ=37)
          gTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCa                                                                         <  6:119924/149‑1 (MQ=37)
              gATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCAGtt                                                                      >  6:87769/1‑148 (MQ=37)
              gATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGttt                                                                     >  3:6146/1‑149 (MQ=37)
               aTCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACTCCAGttt                                                                     >  8:176878/1‑148 (MQ=37)
               aTCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGttt                                                                     >  7:22589/1‑148 (MQ=37)
                tCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCa                                                                   <  7:220651/149‑1 (MQ=37)
                tCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTc                                                                    >  2:85869/1‑148 (MQ=37)
                tCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCa                                                                   <  7:77084/149‑1 (MQ=37)
                tCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCa                                                                   <  5:12250/149‑1 (MQ=37)
                tCCCGCTGTGCTCCACCACTTTCGCCGTACGCAGTTACTTCTCAGAACTTAGAACTGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCACTTTCa                                                                   <  8:170819/149‑1 (MQ=11)
                   cGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACAAGTTTCActg                                                                >  3:320100/1‑149 (MQ=255)
                    gCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGAGATAGATATAGGCTGAACACCAGTTTCActgc                                                               >  5:80341/1‑149 (MQ=255)
                     cTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCActgct                                                              <  7:296419/149‑1 (MQ=37)
                      ttGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCActgctg                                                             >  6:284502/1‑149 (MQ=37)
                      ttGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCActgctg                                                             >  7:71565/1‑149 (MQ=37)
                        ggCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCActgctg                                                             <  3:312488/147‑1 (MQ=37)
                         gCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCtggtg                                                          <  4:250851/149‑1 (MQ=37)
                         gCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCtggtg                                                          <  4:95017/149‑1 (MQ=37)
                          cTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCtggtg                                                          >  2:267497/1‑148 (MQ=37)
                           tCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCTGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGa                                                        >  5:182506/1‑149 (MQ=37)
                           tCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGa                                                        >  6:209803/1‑149 (MQ=37)
                            ccaccaCTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGa                                                        >  7:204056/1‑148 (MQ=37)
                             caccacTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATc                                                      <  1:267497/149‑1 (MQ=37)
                               ccacTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAg                                                    >  3:48372/1‑149 (MQ=37)
                               ccacTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAg                                                    <  7:193392/149‑1 (MQ=37)
                                cacTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAgg                                                   <  5:215010/149‑1 (MQ=37)
                                cacTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAgg                                                   >  3:81486/1‑149 (MQ=37)
                                cacTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAgg                                                   <  6:370199/149‑1 (MQ=37)
                                cacTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAgg                                                   >  3:271289/1‑149 (MQ=37)
                                 acTTTCGCCGTTCGCAGTTAATTGTCATAACTTATAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCATTTG‑TCA‑‑GATCGTTCTTTAAAATATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAgg                                                   <  1:289668/149‑1 (MQ=11)
                                 acTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGc                                                  <  1:372631/149‑1 (MQ=37)
                                 acTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGc                                                  <  6:62384/149‑1 (MQ=37)
                                   tttCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGATGGTGGATCAGGCt                                                 >  3:39487/1‑148 (MQ=255)
                                     tCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTaa                                               <  8:24659/148‑1 (MQ=37)
                                     tCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTaa                                               <  1:46694/148‑1 (MQ=37)
                                     tCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTa                                                <  6:96681/147‑1 (MQ=37)
                                       gCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAgg                                             <  7:333597/148‑1 (MQ=37)
                                       gCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGAGCAGGCTAAgg                                             <  3:192481/148‑1 (MQ=37)
                                       gCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGAGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAgg                                             <  6:80341/148‑1 (MQ=255)
                                              gCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAAttt                                     >  7:323632/1‑149 (MQ=37)
                                                aGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtgt                                   >  2:199196/1‑149 (MQ=37)
                                                aGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtgt                                   <  8:323632/149‑1 (MQ=37)
                                                 gTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtgtg                                  <  5:253081/149‑1 (MQ=37)
                                                  ttACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGa                                 >  4:212195/1‑149 (MQ=25)
                                                      ttGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCAGGGATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGt                               >  1:289304/1‑149 (MQ=255)
                                                      ttGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTc                             >  4:151909/1‑149 (MQ=21)
                                                      ttGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTc                             >  6:183462/1‑149 (MQ=21)
                                                      ttGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTc                             >  5:325204/1‑149 (MQ=34)
                                                        gTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAATATTTGTGAGTTCTg                           >  2:338949/1‑149 (MQ=21)
                                                        gTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTg                           >  2:20813/1‑149 (MQ=21)
                                                        gTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTc                             <  4:238226/147‑1 (MQ=34)
                                                        gTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCt                            <  6:325204/148‑1 (MQ=34)
                                                         tCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGATCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGAATGAACACCAGTTTCACTGATGGTGGATCAGGCTaa                                               >  1:279470/1‑129 (MQ=255)
                                                         tCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGc                          >  8:121704/1‑149 (MQ=21)
                                                         tCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGc                          >  7:308683/1‑149 (MQ=21)
                                                         tCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGc                          >  3:281726/1‑149 (MQ=21)
                                                          cAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGc                          <  7:213722/148‑1 (MQ=34)
                                                           aGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCt                         <  8:22589/148‑1 (MQ=34)
                                                           aGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCt                         <  4:6146/148‑1 (MQ=34)
                                                              aCTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGaa                     <  5:284502/149‑1 (MQ=34)
                                                               cTTAGAAATGAACATTC‑AGCTCGATTTTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGaaa                    <  1:34979/149‑1 (MQ=255)
                                                                ttAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGa                      >  6:102944/1‑146 (MQ=34)
                                                                ttAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCAATGATGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGATCGa                      >  2:335367/1‑146 (MQ=255)
                                                                ttAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTATTG‑ACA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACAGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGATCTGCTCGa                      >  5:174642/1‑146 (MQ=255)
                                                                ccaGAAATCAACATTCGAGCT‑GAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGa                      >  5:198760/3‑146 (MQ=11)
                                                                  aGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAaga                  <  5:161812/148‑1 (MQ=21)
                                                                  aGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGaaa                    <  5:102944/146‑1 (MQ=34)
                                                                  aGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGaaa                    <  1:335367/146‑1 (MQ=34)
                                                                   gAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTctttcgag                 <  7:31105/148‑8 (MQ=21)
                                                                   gAAATCAACATTCGAGCT‑GAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCAGTGGTGGTGGAGCAGGCTAAGGTAAAATTTGTGAGTTATGCTCGaaactg                 >  1:2611/1‑145 (MQ=11)
                                                                     aaTGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGATGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCa               >  6:158132/1‑148 (MQ=21)
                                                                      aTGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTATGCTCGAAAGAGCAAc             >  3:306407/1‑149 (MQ=21)
                                                                        gAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACg            >  8:298596/1‑148 (MQ=34)
                                                                         aaCATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGc                          <  7:314342/133‑1 (MQ=34)
                                                                         aaCATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGc                          >  8:314342/1‑133 (MQ=34)
                                                                         aaCATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTAACTGCTGGTGGATCAGGATAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTa          >  5:139196/1‑149 (MQ=21)
                                                                          aCATTC‑GGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTa          <  3:229061/148‑1 (MQ=17)
                                                                          aCATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCTGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTAc         <  6:182506/149‑1 (MQ=21)
                                                                          aCATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTAc         <  5:173984/149‑1 (MQ=21)
                                                                            aTTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACg        >  2:174856/1‑148 (MQ=34)
                                                                              tC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTa                                           <  6:221125/111‑1 (MQ=21)
                                                                              tC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTa                                           >  5:221125/1‑111 (MQ=21)
                                                                               c‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAAt     <  7:338230/148‑1 (MQ=17)
                                                                                 aGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAAttt   >  7:111797/1‑149 (MQ=32)
                                                                                  gCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAAtttt  >  2:308488/1‑149 (MQ=14)
                                                                                                                                                         |                                                                                 
GAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTG‑TCA‑‑GATCGTTCTTTAAAA‑ATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTT  >  NC_002947/699574‑699803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑