Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 707,561 T→C 8.3% intergenic (‑213/‑38) rpsT ← / → murJ 30S ribosomal protein S20/lipid II flippase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947707,5610TC 8.3% 204.9 / 7.3 72intergenic (‑213/‑38)rpsT/murJ30S ribosomal protein S20/lipid II flippase
Reads supporting (aligned to +/- strand):  ref base T (32/34);  new base C (3/3);  total (35/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01

GTCGAGCGGATGTCAAGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCTGGTGTC  >  NC_002947/707414‑707706
                                                                                                                                                   |                                                                                                                                                 
gTCGAGCGGATGTCAAGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTc                                                                                                                                                  <  5:382738/149‑1 (MQ=255)
 tCGAGCGGATGTCAAGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCt                                                                                                                                                 <  8:255216/149‑1 (MQ=255)
   gAGCGGATGTCAAGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTg                                                                                                                                                <  6:67992/148‑1 (MQ=255)
   gAGCGGATGTCAAGGCCACCTGAAAGATTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCATGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTgg                                                                                                                                               >  1:9209/1‑149 (MQ=255)
            tCAAGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCct                                                                                                                                      >  2:13724/1‑149 (MQ=255)
            tCAAGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGCCAGACAGCGTcc                                                                                                                                       <  5:170613/148‑1 (MQ=255)
              aaGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCctct                                                                                                                                    <  8:43266/149‑1 (MQ=255)
              aaGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCctct                                                                                                                                    <  7:304606/149‑1 (MQ=255)
              aaGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGCCAGACAGCGTccgaa                                                                                                                                    <  2:346684/149‑4 (MQ=255)
               aGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTg                                                                                                                                   <  3:30283/149‑1 (MQ=255)
                  ccACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCa                                                                                                                                >  2:213147/1‑149 (MQ=255)
                        gCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCg                                                                                                                          >  6:275969/1‑149 (MQ=255)
                                 cgccgAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCttt                                                                                                                 <  1:42515/149‑1 (MQ=255)
                                  gccgAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCtttt                                                                                                                >  3:286819/1‑149 (MQ=255)
                                  gccgAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCtttt                                                                                                                <  1:213147/149‑1 (MQ=255)
                                  gccgAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCtttt                                                                                                                <  2:138992/149‑1 (MQ=255)
                                   ccgAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTc                                                                                                               >  4:136773/1‑149 (MQ=255)
                                        cGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGa                                                                                                          <  4:286819/149‑1 (MQ=255)
                                                caacaaGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCt                                                                                                   >  8:333322/1‑148 (MQ=255)
                                                      gCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATcc                                                                                            >  4:292931/1‑149 (MQ=255)
                                                       cGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATccc                                                                                           >  3:343424/1‑149 (MQ=255)
                                                        gggAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTgg                                                                                                                                               <  6:175950/96‑1 (MQ=255)
                                                        gggAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTgg                                                                                                                                               >  5:175950/1‑96 (MQ=255)
                                                        gggAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCt                                                                                          <  4:265791/149‑1 (MQ=255)
                                                        gggAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCt                                                                                          <  3:136773/149‑1 (MQ=255)
                                                        gggAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCt                                                                                          <  1:326498/149‑1 (MQ=255)
                                                        gggAAAGATTCCTTTCTGCTGCGCGACCTGTACACTAGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCt                                                                                          <  2:9209/149‑1 (MQ=255)
                                                          gAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGc                                                                                                                           <  7:157440/114‑1 (MQ=255)
                                                          gAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGc                                                                                                                           >  8:157440/1‑114 (MQ=255)
                                                          gAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTgg                                                                                        <  6:289229/149‑1 (MQ=255)
                                                          gAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTgg                                                                                        <  3:328568/149‑1 (MQ=255)
                                                               aTTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCg                                                                                                              >  2:346047/1‑122 (MQ=255)
                                                               aTTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCg                                                                                                              <  1:346047/122‑1 (MQ=255)
                                                                  ccTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGt                                                                                 >  8:11687/1‑148 (MQ=255)
                                                                    tttCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAg                                                                              >  3:251598/1‑149 (MQ=255)
                                                                        tgctgcGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTc                                                                           >  8:33577/1‑148 (MQ=255)
                                                                            gcgcGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCa                                                                      >  6:317339/1‑149 (MQ=255)
                                                                            gcgcGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCa                                                                      >  1:144953/1‑149 (MQ=255)
                                                                             cgcgACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCAc                                                                     <  7:11687/149‑1 (MQ=255)
                                                                               cgACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCa                                                                   <  5:215611/149‑1 (MQ=255)
                                                                                gACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCAt                                                                  <  5:275969/149‑1 (MQ=255)
                                                                                  ccTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGa                                                                >  7:169375/1‑149 (MQ=255)
                                                                                   cTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGAt                                                               >  2:324793/1‑149 (MQ=255)
                                                                                       acacTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTcg                                                           >  3:331360/1‑149 (MQ=255)
                                                                                           tCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCggg                                                       >  1:367835/1‑149 (MQ=255)
                                                                                             gggAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCGGCAGTAAGCGCGATCCCCAGGATTTCGCGGGTg                                                     >  3:352046/1‑149 (MQ=255)
                                                                                                    tttGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGc                                               <  1:13724/148‑1 (MQ=255)
                                                                                                    tttGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCACGATCACCATGATTTCGCGGGTGCTGGGCt                                              >  6:43218/1‑149 (MQ=255)
                                                                                                       ggCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCttt                                            <  8:169375/148‑1 (MQ=255)
                                                                                                        gCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGt                                          >  6:209166/1‑149 (MQ=255)
                                                                                                        gCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGt                                          >  3:67461/1‑149 (MQ=255)
                                                                                                            cccTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTTCTCAAATCCCTGGCTGCAGTAAGCTCGATTACCATGATTTCGCGGGTGCTGGGCTTTGTTcgc                                      <  2:308379/149‑1 (MQ=255)
                                                                                                            cccTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTc                                        <  4:67461/147‑1 (MQ=255)
                                                                                                                   aaGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCa                               <  4:251598/149‑1 (MQ=255)
                                                                                                                          gggAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGc                        <  3:292931/149‑1 (MQ=255)
                                                                                                                                 tCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATc                 >  7:195630/1‑149 (MQ=255)
                                                                                                                                 tCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATc                 >  3:129976/1‑149 (MQ=255)
                                                                                                                                 tCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTGTGTTCGCGACCGCATCCTGGCGCGCATc                 >  4:321464/1‑149 (MQ=255)
                                                                                                                                   gCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCtt               >  3:135441/1‑149 (MQ=255)
                                                                                                                                    cacaTTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTg                                                                                     >  6:59632/1‑78 (MQ=255)
                                                                                                                                    cacaTTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTg                                                                                     <  5:59632/78‑1 (MQ=255)
                                                                                                                                    cacaTTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCttt              >  1:232734/1‑149 (MQ=255)
                                                                                                                                     acaTTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTg             <  2:367835/149‑1 (MQ=255)
                                                                                                                                     acaTTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTg             <  1:324793/149‑1 (MQ=255)
                                                                                                                                         gaggACGCTGCCAGACAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGc         >  6:21482/3‑149 (MQ=255)
                                                                                                                                          aggACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATACCTGGCTGCAGTAACCTCGATCACCAAGATTTCGCGCGTGCTGGGCTTTCTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCt        >  3:16940/2‑149 (MQ=255)
                                                                                                                                          aggACGCTGCCAGACAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGt           <  7:63455/145‑1 (MQ=255)
                                                                                                                                          aggACGCTGCCAGACAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGt           >  8:63455/2‑146 (MQ=255)
                                                                                                                                          aggACGCTGCCAGACAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCt        >  5:110539/2‑149 (MQ=255)
                                                                                                                                           ggACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCTg       <  7:333322/149‑1 (MQ=255)
                                                                                                                                           ggACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCGCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCTg       <  5:43218/149‑1 (MQ=255)
                                                                                                                                                cTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCTGGTGTc  <  4:343424/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GTCGAGCGGATGTCAAGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCTGGTGTC  >  NC_002947/707414‑707706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑