Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 707,563 T→A 8.4% intergenic (‑215/‑36) rpsT ← / → murJ 30S ribosomal protein S20/lipid II flippase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947707,5630TA 8.4% 202.9 / 8.0 71intergenic (‑215/‑36)rpsT/murJ30S ribosomal protein S20/lipid II flippase
Reads supporting (aligned to +/- strand):  ref base T (33/32);  new base A (3/3);  total (36/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01

TCGAGCGGATGTCAAGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCTGGTGTCGC  >  NC_002947/707415‑707708
                                                                                                                                                    |                                                                                                                                                  
tCGAGCGGATGTCAAGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCt                                                                                                                                                    <  8:255216/149‑1 (MQ=255)
  gAGCGGATGTCAAGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTg                                                                                                                                                   <  6:67992/148‑1 (MQ=255)
  gAGCGGATGTCAAGGCCACCTGAAAGATTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCATGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTgg                                                                                                                                                  >  1:9209/1‑149 (MQ=255)
           tCAAGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCct                                                                                                                                         >  2:13724/1‑149 (MQ=255)
           tCAAGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGCCAGACAGCGTcc                                                                                                                                          <  5:170613/148‑1 (MQ=255)
             aaGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCctct                                                                                                                                       <  8:43266/149‑1 (MQ=255)
             aaGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCctct                                                                                                                                       <  7:304606/149‑1 (MQ=255)
             aaGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGCCAGACAGCGTccgaa                                                                                                                                       <  2:346684/149‑4 (MQ=255)
              aGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTg                                                                                                                                      <  3:30283/149‑1 (MQ=255)
                 ccACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCa                                                                                                                                   >  2:213147/1‑149 (MQ=255)
                       gCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCg                                                                                                                             >  6:275969/1‑149 (MQ=255)
                                cgccgAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCttt                                                                                                                    <  1:42515/149‑1 (MQ=255)
                                 gccgAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCtttt                                                                                                                   >  3:286819/1‑149 (MQ=255)
                                 gccgAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCtttt                                                                                                                   <  2:138992/149‑1 (MQ=255)
                                 gccgAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCtttt                                                                                                                   <  1:213147/149‑1 (MQ=255)
                                  ccgAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTc                                                                                                                  >  4:136773/1‑149 (MQ=255)
                                       cGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGa                                                                                                             <  4:286819/149‑1 (MQ=255)
                                               caacaaGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCt                                                                                                      >  8:333322/1‑148 (MQ=255)
                                                     gCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATcc                                                                                               >  4:292931/1‑149 (MQ=255)
                                                      cGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATccc                                                                                              >  3:343424/1‑149 (MQ=255)
                                                       gggAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTgg                                                                                                                                                  >  5:175950/1‑96 (MQ=255)
                                                       gggAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTgg                                                                                                                                                  <  6:175950/96‑1 (MQ=255)
                                                       gggAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCt                                                                                             <  4:265791/149‑1 (MQ=255)
                                                       gggAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCt                                                                                             <  3:136773/149‑1 (MQ=255)
                                                       gggAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCt                                                                                             <  1:326498/149‑1 (MQ=255)
                                                       gggAAAGATTCCTTTCTGCTGCGCGACCTGTACACTAGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCt                                                                                             <  2:9209/149‑1 (MQ=255)
                                                         gAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGc                                                                                                                              <  7:157440/114‑1 (MQ=255)
                                                         gAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGc                                                                                                                              >  8:157440/1‑114 (MQ=255)
                                                         gAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTg‑g                                                                                          <  3:328568/149‑1 (MQ=255)
                                                         gAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTg‑g                                                                                          <  6:289229/149‑1 (MQ=255)
                                                              aTTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCg                                                                                                                 <  1:346047/122‑1 (MQ=255)
                                                              aTTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCg                                                                                                                 >  2:346047/1‑122 (MQ=255)
                                                                 ccTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGt                                                                                   >  8:11687/1‑148 (MQ=255)
                                                                   tttCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAg                                                                                >  3:251598/1‑149 (MQ=255)
                                                                       tgctgcGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTc                                                                             >  8:33577/1‑148 (MQ=255)
                                                                           gcgcGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCa                                                                        >  6:317339/1‑149 (MQ=255)
                                                                           gcgcGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCa                                                                        >  1:144953/1‑149 (MQ=255)
                                                                            cgcgACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCAc                                                                       <  7:11687/149‑1 (MQ=255)
                                                                              cgACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCa                                                                     <  5:215611/149‑1 (MQ=255)
                                                                               gACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCAt                                                                    <  5:275969/149‑1 (MQ=255)
                                                                                 ccTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGa                                                                  >  7:169375/1‑149 (MQ=255)
                                                                                  cTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGAt                                                                 >  2:324793/1‑149 (MQ=255)
                                                                                      acacTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTcg                                                             >  3:331360/1‑149 (MQ=255)
                                                                                          tCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCggg                                                         >  1:367835/1‑149 (MQ=255)
                                                                                            gggAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCGGCAGTAAGCGCGATCCCCAGGATTTCGCGGGTg                                                       >  3:352046/1‑149 (MQ=255)
                                                                                                   tttGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGc                                                 <  1:13724/148‑1 (MQ=255)
                                                                                                   tttGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCACGATCACCATGATTTCGCGGGTGCTGGGCt                                                >  6:43218/1‑149 (MQ=255)
                                                                                                      ggCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCttt                                              <  8:169375/148‑1 (MQ=255)
                                                                                                       gCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGt                                            >  3:67461/1‑149 (MQ=255)
                                                                                                       gCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGt                                            >  6:209166/1‑149 (MQ=255)
                                                                                                           cccTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTTCTCAAATCCCTG‑GCTGCAGTAAGCTCGATTACCATGATTTCGCGGGTGCTGGGCTTTGTTcgc                                        <  2:308379/149‑1 (MQ=255)
                                                                                                           cccTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTc                                          <  4:67461/147‑1 (MQ=255)
                                                                                                                  aaGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCa                                 <  4:251598/149‑1 (MQ=255)
                                                                                                                         gggAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGc                          <  3:292931/149‑1 (MQ=255)
                                                                                                                                tCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATc                   >  7:195630/1‑149 (MQ=255)
                                                                                                                                tCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATc                   >  3:129976/1‑149 (MQ=255)
                                                                                                                                tCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTGTGTTCGCGACCGCATCCTGGCGCGCATc                   >  4:321464/1‑149 (MQ=255)
                                                                                                                                  gCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCtt                 >  3:135441/1‑149 (MQ=255)
                                                                                                                                   cacaTTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTg                                                                                       >  6:59632/1‑78 (MQ=255)
                                                                                                                                   cacaTTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTg                                                                                       <  5:59632/78‑1 (MQ=255)
                                                                                                                                   cacaTTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCttt                >  1:232734/1‑149 (MQ=255)
                                                                                                                                    acaTTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTg               <  2:367835/149‑1 (MQ=255)
                                                                                                                                    acaTTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTg               <  1:324793/149‑1 (MQ=255)
                                                                                                                                        gaggACGCTGCCAGACAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGc           >  6:21482/3‑149 (MQ=255)
                                                                                                                                         aggACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATACCTG‑GCTGCAGTAACCTCGATCACCAAGATTTCGCGCGTGCTGGGCTTTCTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCt          >  3:16940/2‑149 (MQ=255)
                                                                                                                                         aggACGCTGCCAGACAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGt             <  7:63455/145‑1 (MQ=255)
                                                                                                                                         aggACGCTGCCAGACAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGt             >  8:63455/2‑146 (MQ=255)
                                                                                                                                         aggACGCTGCCAGACAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCt          >  5:110539/2‑149 (MQ=255)
                                                                                                                                          ggACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCTg         <  7:333322/149‑1 (MQ=255)
                                                                                                                                          ggACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCGCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCTg         <  5:43218/149‑1 (MQ=255)
                                                                                                                                               cTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCTGGTGTc    <  4:343424/149‑1 (MQ=255)
                                                                                                                                                   cTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGAGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCTGGTGTCGc  >  1:293801/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TCGAGCGGATGTCAAGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCTGGTGTCGC  >  NC_002947/707415‑707708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑