Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 707,565 G→A 8.9% intergenic (‑217/‑34) rpsT ← / → murJ 30S ribosomal protein S20/lipid II flippase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947707,5650GA 8.9% 187.9 / 7.9 67intergenic (‑217/‑34)rpsT/murJ30S ribosomal protein S20/lipid II flippase
Reads supporting (aligned to +/- strand):  ref base G (31/30);  new base A (3/3);  total (34/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.70e-01

GAGCGGATGTCAAGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCTGGTGTCGCTACCG  >  NC_002947/707417‑707713
                                                                                                                                                    |                                                                                                                                                     
gAGCGGATGTCAAGGCCACCTGAAAGATTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCATGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTgg                                                                                                                                                       >  1:9209/1‑149 (MQ=255)
         tCAAGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCct                                                                                                                                              >  2:13724/1‑149 (MQ=255)
         tCAAGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGCCAGACAGCGTcc                                                                                                                                               <  5:170613/148‑1 (MQ=255)
           aaGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCctct                                                                                                                                            <  7:304606/149‑1 (MQ=255)
           aaGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCctct                                                                                                                                            <  8:43266/149‑1 (MQ=255)
           aaGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGCCAGACAGCGTccgaa                                                                                                                                            <  2:346684/149‑4 (MQ=255)
            aGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTg                                                                                                                                           <  3:30283/149‑1 (MQ=255)
               ccACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCa                                                                                                                                        >  2:213147/1‑149 (MQ=255)
                     gCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCg                                                                                                                                  >  6:275969/1‑149 (MQ=255)
                              cgccgAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCttt                                                                                                                         <  1:42515/149‑1 (MQ=255)
                               gccgAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCtttt                                                                                                                        <  2:138992/149‑1 (MQ=255)
                               gccgAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCtttt                                                                                                                        >  3:286819/1‑149 (MQ=255)
                               gccgAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCtttt                                                                                                                        <  1:213147/149‑1 (MQ=255)
                                ccgAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTc                                                                                                                       >  4:136773/1‑149 (MQ=255)
                                     cGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGa                                                                                                                  <  4:286819/149‑1 (MQ=255)
                                             caacaaGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCt                                                                                                           >  8:333322/1‑148 (MQ=255)
                                                   gCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATcc                                                                                                    >  4:292931/1‑149 (MQ=255)
                                                    cGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATccc                                                                                                   >  3:343424/1‑149 (MQ=255)
                                                     gggAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTgg                                                                                                                                                       <  6:175950/96‑1 (MQ=255)
                                                     gggAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTgg                                                                                                                                                       >  5:175950/1‑96 (MQ=255)
                                                     gggAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCt                                                                                                  <  3:136773/149‑1 (MQ=255)
                                                     gggAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCt                                                                                                  <  1:326498/149‑1 (MQ=255)
                                                     gggAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCt                                                                                                  <  4:265791/149‑1 (MQ=255)
                                                     gggAAAGATTCCTTTCTGCTGCGCGACCTGTACACTAGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCt                                                                                                  <  2:9209/149‑1 (MQ=255)
                                                       gAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGc                                                                                                                                   >  8:157440/1‑114 (MQ=255)
                                                       gAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGc                                                                                                                                   <  7:157440/114‑1 (MQ=255)
                                                       gAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTg‑g                                                                                               <  6:289229/149‑1 (MQ=255)
                                                       gAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTg‑g                                                                                               <  3:328568/149‑1 (MQ=255)
                                                            aTTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCg                                                                                                                      >  2:346047/1‑122 (MQ=255)
                                                            aTTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCg                                                                                                                      <  1:346047/122‑1 (MQ=255)
                                                               ccTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGt                                                                                        >  8:11687/1‑148 (MQ=255)
                                                                 tttCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAg                                                                                     >  3:251598/1‑149 (MQ=255)
                                                                     tgctgcGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTc                                                                                  >  8:33577/1‑148 (MQ=255)
                                                                         gcgcGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCa                                                                             >  6:317339/1‑149 (MQ=255)
                                                                         gcgcGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCa                                                                             >  1:144953/1‑149 (MQ=255)
                                                                          cgcgACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCAc                                                                            <  7:11687/149‑1 (MQ=255)
                                                                            cgACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCa                                                                          <  5:215611/149‑1 (MQ=255)
                                                                             gACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCAt                                                                         <  5:275969/149‑1 (MQ=255)
                                                                               ccTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGa                                                                       >  7:169375/1‑149 (MQ=255)
                                                                                cTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGAt                                                                      >  2:324793/1‑149 (MQ=255)
                                                                                    acacTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTcg                                                                  >  3:331360/1‑149 (MQ=255)
                                                                                        tCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCggg                                                              >  1:367835/1‑149 (MQ=255)
                                                                                          gggAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCGGCAGTAAGCGCGATCCCCAGGATTTCGCGGGTg                                                            >  3:352046/1‑149 (MQ=255)
                                                                                                 tttGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGc                                                      <  1:13724/148‑1 (MQ=255)
                                                                                                 tttGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCACGATCACCATGATTTCGCGGGTGCTGGGCt                                                     >  6:43218/1‑149 (MQ=255)
                                                                                                    ggCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCttt                                                   <  8:169375/148‑1 (MQ=255)
                                                                                                     gCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGt                                                 >  3:67461/1‑149 (MQ=255)
                                                                                                     gCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGt                                                 >  6:209166/1‑149 (MQ=255)
                                                                                                         cccTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTTCTCAAATCCCTG‑GCTGCAGTAAGCTCGATTACCATGATTTCGCGGGTGCTGGGCTTTGTTcgc                                             <  2:308379/149‑1 (MQ=255)
                                                                                                         cccTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTc                                               <  4:67461/147‑1 (MQ=255)
                                                                                                                aaGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCa                                      <  4:251598/149‑1 (MQ=255)
                                                                                                                       gggAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGc                               <  3:292931/149‑1 (MQ=255)
                                                                                                                              tCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATc                        >  3:129976/1‑149 (MQ=255)
                                                                                                                              tCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATc                        >  7:195630/1‑149 (MQ=255)
                                                                                                                              tCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTGTGTTCGCGACCGCATCCTGGCGCGCATc                        >  4:321464/1‑149 (MQ=255)
                                                                                                                                gCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCtt                      >  3:135441/1‑149 (MQ=255)
                                                                                                                                 cacaTTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTg                                                                                            <  5:59632/78‑1 (MQ=255)
                                                                                                                                 cacaTTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTg                                                                                            >  6:59632/1‑78 (MQ=255)
                                                                                                                                 cacaTTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCttt                     >  1:232734/1‑149 (MQ=255)
                                                                                                                                  acaTTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTg                    <  2:367835/149‑1 (MQ=255)
                                                                                                                                  acaTTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTg                    <  1:324793/149‑1 (MQ=255)
                                                                                                                                      gaggACGCTGCCAGACAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGc                >  6:21482/3‑149 (MQ=255)
                                                                                                                                       aggACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATACCTG‑GCTGCAGTAACCTCGATCACCAAGATTTCGCGCGTGCTGGGCTTTCTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCt               >  3:16940/2‑149 (MQ=255)
                                                                                                                                       aggACGCTGCCAGACAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGt                  <  7:63455/145‑1 (MQ=255)
                                                                                                                                       aggACGCTGCCAGACAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGt                  >  8:63455/2‑146 (MQ=255)
                                                                                                                                       aggACGCTGCCAGACAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCt               >  5:110539/2‑149 (MQ=255)
                                                                                                                                        ggACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCTg              <  7:333322/149‑1 (MQ=255)
                                                                                                                                        ggACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCGCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCTg              <  5:43218/149‑1 (MQ=255)
                                                                                                                                             cTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCTGGTGTc         <  4:343424/149‑1 (MQ=255)
                                                                                                                                                 cTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTGAGCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCTGGTGTCGc       >  1:293801/1‑148 (MQ=255)
                                                                                                                                                    gCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCTAGTGTCGCTACCg  <  2:144953/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GAGCGGATGTCAAGGCCACCTGCAAGGTTCCGCCGAGCGGTGCGCCAACAAGCGGGAAAGATTCCTTTCTGCTGCGCGACCTGTACACTCGGGAATTTTTGGCTCCCCTGCGAAGGCGCGGGAGTATCGCACATTCGGACGCTGTCTGGCAGCGTCCTCTGTCATTGGCGTGAATCTTTTCGATGAACCTGCTCAAATCCCTG‑GCTGCAGTAAGCTCGATCACCATGATTTCGCGGGTGCTGGGCTTTGTTCGCGACACCATCCTGGCCCGCATCTTTGGTGCTGGTGTCGCTACCG  >  NC_002947/707417‑707713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑