Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 726,622 G→C 9.4% F44L (TTC→TTG) ‡ PP_0620 ← GntR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947726,6220GC 9.4% 194.6 / 9.5 64F44L (TTC→TTG) ‡PP_0620GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (22/36);  new base C (3/3);  total (25/39)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

AGTGCAGGTAGTTGCGCAGTGCGCGGCTGGCTGGTTCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCTTCTGGCGCTTGCGTTTGGGTTTGAGCATTTCGCTATTTTTCATAT  >  NC_002947/726474‑726770
                                                                                                                                                    |                                                                                                                                                    
aGTGCAGGTAGTTGCGCAGTGCGCGGCTGGCTGGTGCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCg                                                                                                                                                      <  3:104661/149‑1 (MQ=255)
    cAGGTAGTTGCGCAGTGCGCGGCTGGCTGGTTCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAAt                                                                                                                                                  <  7:65910/149‑1 (MQ=255)
        tAGTTGCGCAGTGCGCGGCTGGCTGGTTCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTc                                                                                                                                              <  2:122344/149‑1 (MQ=255)
           ttGCGCAGTGCGCGGCTGGCTGGTTCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTTCGAATGCTCCaa                                                                                                                                           <  2:325652/149‑2 (MQ=255)
            tGCGCAGTGCGCGGCTGGCTGGTTCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATc                                                                                                                                          <  8:13473/149‑1 (MQ=255)
             gcgcAGTGCGCGGCTGGCTGGTTCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATc                                                                                                                                          <  8:21167/148‑1 (MQ=255)
              cgcAGTGCGCGGCTGGCTGGTTCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCa                                                                                                                                         <  8:296305/148‑1 (MQ=255)
              cgcAGTGCGCGGCTGGCTGGTTCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCa                                                                                                                                         <  4:259914/148‑1 (MQ=255)
               gcAGTGCGCGGCTGGCTGGTTCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGc                                                                                                                                       >  4:142261/1‑149 (MQ=255)
                    gcgcGGCTGGCTGGTTCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCg                                                                                                                                  >  5:88336/1‑149 (MQ=255)
                        ggctggctGGGTCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCg                                                                                                                              <  7:122700/149‑1 (MQ=255)
                          ctggctggTTCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGt                                                                                                                            <  7:291322/149‑1 (MQ=255)
                             gctggTTCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTagg                                                                                                                         >  8:55617/1‑149 (MQ=255)
                              ctggTTCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTaggc                                                                                                                        <  4:66542/149‑1 (MQ=255)
                               tggTTCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCAt                                                                                                                                           <  3:28096/129‑1 (MQ=255)
                               tggTTCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCAt                                                                                                                                           >  4:28096/1‑129 (MQ=255)
                                  ttCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGc                                                                                                                                                <  6:188380/121‑1 (MQ=255)
                                     ggTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGt                                                                                                                 >  1:122708/1‑149 (MQ=255)
                                      gTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAACTGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTc                                                                                                                >  4:286990/1‑149 (MQ=255)
                                          ccACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGACGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTaggcag                                                                                                                      <  3:233191/139‑1 (MQ=255)
                                          ccACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGACGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTaggcag                                                                                                                      >  4:233191/1‑139 (MQ=255)
                                           cACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCgg                                                                                                           >  7:78889/1‑149 (MQ=255)
                                           cACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCgg                                                                                                           >  6:286277/1‑149 (MQ=255)
                                            aCTTCGTTCAGGTAGGCGTCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCggg                                                                                                          <  2:311532/149‑1 (MQ=255)
                                                cGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTg                                                                                                       <  4:71223/148‑1 (MQ=255)
                                                 gTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCa                                                                                                     <  3:260668/149‑1 (MQ=255)
                                                  ttCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAg                                                                                                    >  8:216846/1‑149 (MQ=255)
                                                   tCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGG‑‑GGTGCCCTTGGAGCATTCg                                                                                                                                                      <  4:199757/96‑1 (MQ=255)
                                                   tCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGG‑‑GGTGCCCTTGGAGCATTCg                                                                                                                                                      >  3:199757/1‑96 (MQ=255)
                                                    cAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAgc                                                                                                   <  1:58034/148‑1 (MQ=255)
                                                       gTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCtt                                                                                               >  4:271828/1‑149 (MQ=255)
                                                        tAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTc                                                                                              <  7:81173/149‑1 (MQ=255)
                                                               ccccGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGAc                                                                                        >  1:163653/1‑148 (MQ=255)
                                                                 ccGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGAt                                                                                     <  5:286277/149‑1 (MQ=255)
                                                                    gTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGa                                                                                  <  6:34493/149‑1 (MQ=255)
                                                                        ggCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAgcgc                                                                              <  7:165676/149‑1 (MQ=255)
                                                                          ccGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCtt                                                                            <  6:88336/149‑1 (MQ=255)
                                                                           cGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTg                                                                           <  5:17125/149‑1 (MQ=255)
                                                                                 gggTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGAt                                                                     <  8:78889/149‑1 (MQ=255)
                                                                                  ggTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGAtt                                                                    <  2:163653/149‑1 (MQ=255)
                                                                                  ggTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGAtt                                                                    <  7:55617/149‑1 (MQ=255)
                                                                                  ggTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGAtt                                                                    >  3:320968/1‑149 (MQ=255)
                                                                                   gTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTTCGAATGCTCCaag                                                                                                                                          <  5:271012/78‑3 (MQ=38)
                                                                                   gTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTTCGAATGCTCCaag                                                                                                                                          >  6:271012/1‑76 (MQ=38)
                                                                                     gCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTcga                                                                 <  8:81067/149‑1 (MQ=255)
                                                                                      cTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTcga                                                                 <  4:320968/148‑1 (MQ=255)
                                                                                         aCCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGAt                                                             >  3:26129/1‑149 (MQ=255)
                                                                                           cAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATca                                                           >  5:161366/1‑149 (MQ=255)
                                                                                                       ttcgaGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCt                                               >  5:272165/1‑149 (MQ=255)
                                                                                                       ttcgaGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCt                                               >  3:247573/1‑149 (MQ=255)
                                                                                                       ttcgaGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGAGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACa                                                  >  7:161669/1‑146 (MQ=255)
                                                                                                       ttcgaGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGAGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACa                                                  <  8:161669/146‑1 (MQ=255)
                                                                                                           aGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCTCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCTTCTg                                           <  4:158019/149‑1 (MQ=255)
                                                                                                               ccTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCTTCTGgcgc                                       <  3:271828/149‑1 (MQ=255)
                                                                                                                         ttCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCTTCTGGCGCTTGCGTTTgg                             >  2:44866/1‑149 (MQ=255)
                                                                                                                           cgcgGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCTTCTGGCGCTTGCGTTTgg                             <  2:314729/147‑1 (MQ=255)
                                                                                                                            gcgggcggTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCTTCTGGCGCTTGCGTTTGGGtt                          <  7:331130/149‑1 (MQ=255)
                                                                                                                               ggcggTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCTTCTGGCGCTTGCGTTTGGGTTTGa                       <  2:122708/149‑1 (MQ=255)
                                                                                                                               ggcggTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCTTCTGGCGCTTGCGTTTGGGTTTGa                       <  7:216846/149‑1 (MQ=255)
                                                                                                                                    tGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCTTCTGGCGCTTGCGTTTGGGTTTGAGCAtt                  >  4:216118/1‑149 (MQ=255)
                                                                                                                                      cccTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCTTCTGGCGCTTGCGTTTGGGTTTGAGCAttt                 <  3:216118/148‑1 (MQ=255)
                                                                                                                                       tgatGGAGCATTTCGAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCTTCTGGCGCTTGCGTTTGGGTTTGAGCATTTCg               >  2:179025/4‑149 (MQ=255)
                                                                                                                                           ggAGCATTTCGAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCTTCTGGCGCTTGCGTTTGGGTTTGAGCAttt                 <  8:120012/143‑1 (MQ=255)
                                                                                                                                           ggAGCATTTCGAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCTTCTGGCGCTTGCGTTTGGGTTTGAGCAttt                 >  7:120012/1‑143 (MQ=255)
                                                                                                                                                 ttCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTTACTGATTCGACGATCACATCGGACAGCTTCTGGCGCTTGCGTTTGGGTTTGAGCATTTCGCTATTTTTCa     <  3:142261/149‑1 (MQ=255)
                                                                                                                                                 ttCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTCACTGATTCGACGATCACATCGGACAGCTTCTGGCGCTTGCGTTTGGGTTTGAGCATTTCGCTATTTTTCa     >  7:244464/1‑149 (MQ=255)
                                                                                                                                                  tCGTAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCTTCTGGCGCTTGCGTTTGGGTTTGAGCATTTCGCTATTTTTCa     >  6:248160/1‑148 (MQ=255)
                                                                                                                                                    gAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGa                                                    >  6:264732/1‑99 (MQ=255)
                                                                                                                                                    gAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGa                                                    <  5:264732/99‑1 (MQ=255)
                                                                                                                                                    gAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCTTCTGGCGCTTGCGTTTGGGTTTGAGCATTTCGCTATTTTTCatat  >  5:373824/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
AGTGCAGGTAGTTGCGCAGTGCGCGGCTGGCTGGTTCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCTTCTGGCGCTTGCGTTTGGGTTTGAGCATTTCGCTATTTTTCATAT  >  NC_002947/726474‑726770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑