Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 726,623 A→G 9.2% F44S (TTC→TCC) ‡ PP_0620 ← GntR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947726,6230AG 9.2% 182.9 / 7.6 67F44S (TTC→TCC) ‡PP_0620GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (23/37);  new base G (3/3);  total (27/40)
Fisher's exact test for biased strand distribution p-value = 6.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

CAGGTAGTTGCGCAGTGCGCGGCTGGCTGGTTCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCTTCTGGCGCTTGCGTTTGGGTTTGAGCATTTCGCTATTTTTCATAT  >  NC_002947/726478‑726770
                                                                                                                                                 |                                                                                                                                                   
cAGGTAGTTGCGCAGTGCGCGGCTGGCTGGTTCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAAt                                                                                                                                                  <  7:65910/149‑1 (MQ=255)
    tAGTTGCGCAGTGCGCGGCTGGCTGGTTCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTc                                                                                                                                              <  2:122344/149‑1 (MQ=255)
       ttGCGCAGTGCGCGGCTGGCTGGTTCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTTCGAATGCTCCaa                                                                                                                                           <  2:325652/149‑2 (MQ=255)
        tGCGCAGTGCGCGGCTGGCTGGTTCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATc                                                                                                                                          <  8:13473/149‑1 (MQ=255)
         gcgcAGTGCGCGGCTGGCTGGTTCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATc                                                                                                                                          <  8:21167/148‑1 (MQ=255)
          cgcAGTGCGCGGCTGGCTGGTTCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCa                                                                                                                                         <  4:259914/148‑1 (MQ=255)
          cgcAGTGCGCGGCTGGCTGGTTCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCa                                                                                                                                         <  8:296305/148‑1 (MQ=255)
           gcAGTGCGCGGCTGGCTGGTTCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGc                                                                                                                                       >  4:142261/1‑149 (MQ=255)
                gcgcGGCTGGCTGGTTCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCg                                                                                                                                  >  5:88336/1‑149 (MQ=255)
                    ggctggctGGGTCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCg                                                                                                                              <  7:122700/149‑1 (MQ=255)
                      ctggctggTTCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGt                                                                                                                            <  7:291322/149‑1 (MQ=255)
                         gctggTTCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTagg                                                                                                                         >  8:55617/1‑149 (MQ=255)
                          ctggTTCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTaggc                                                                                                                        <  4:66542/149‑1 (MQ=255)
                           tggTTCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCAt                                                                                                                                           >  4:28096/1‑129 (MQ=255)
                           tggTTCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCAt                                                                                                                                           <  3:28096/129‑1 (MQ=255)
                              ttCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGc                                                                                                                                                <  6:188380/121‑1 (MQ=255)
                                 ggTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGt                                                                                                                 >  1:122708/1‑149 (MQ=255)
                                  gTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAACTGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTc                                                                                                                >  4:286990/1‑149 (MQ=255)
                                      ccACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGACGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTaggcag                                                                                                                      <  3:233191/139‑1 (MQ=255)
                                      ccACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGACGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTaggcag                                                                                                                      >  4:233191/1‑139 (MQ=255)
                                       cACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCgg                                                                                                           >  6:286277/1‑149 (MQ=255)
                                       cACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCgg                                                                                                           >  7:78889/1‑149 (MQ=255)
                                        aCTTCGTTCAGGTAGGCGTCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCggg                                                                                                          <  2:311532/149‑1 (MQ=255)
                                            cGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTg                                                                                                       <  4:71223/148‑1 (MQ=255)
                                             gTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCa                                                                                                     <  3:260668/149‑1 (MQ=255)
                                              ttCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAg                                                                                                    >  8:216846/1‑149 (MQ=255)
                                                cAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAgc                                                                                                   <  1:58034/148‑1 (MQ=255)
                                                   gTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCtt                                                                                               >  4:271828/1‑149 (MQ=255)
                                                    tAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTc                                                                                              <  7:81173/149‑1 (MQ=255)
                                                           ccccGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGAc                                                                                        >  1:163653/1‑148 (MQ=255)
                                                             ccGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGAt                                                                                     <  5:286277/149‑1 (MQ=255)
                                                                gTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGa                                                                                  <  6:34493/149‑1 (MQ=255)
                                                                    ggCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAgcgc                                                                              <  7:165676/149‑1 (MQ=255)
                                                                      ccGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCtt                                                                            <  6:88336/149‑1 (MQ=255)
                                                                       cGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTg                                                                           <  5:17125/149‑1 (MQ=255)
                                                                             gggTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGAt                                                                     <  8:78889/149‑1 (MQ=255)
                                                                              ggTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGAtt                                                                    <  7:55617/149‑1 (MQ=255)
                                                                              ggTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGAtt                                                                    >  3:320968/1‑149 (MQ=255)
                                                                              ggTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGAtt                                                                    <  2:163653/149‑1 (MQ=255)
                                                                               gTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTTCGAATGCTCCaag                                                                                                                                          <  5:271012/78‑3 (MQ=38)
                                                                               gTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTTCGAATGCTCCaag                                                                                                                                          >  6:271012/1‑76 (MQ=38)
                                                                                 gCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTcga                                                                 <  8:81067/149‑1 (MQ=255)
                                                                                  cTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTcga                                                                 <  4:320968/148‑1 (MQ=255)
                                                                                     aCCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGAt                                                             >  3:26129/1‑149 (MQ=255)
                                                                                       cAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATca                                                           >  5:161366/1‑149 (MQ=255)
                                                                                                   ttcgaGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCt                                               >  5:272165/1‑149 (MQ=255)
                                                                                                   ttcgaGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCt                                               >  3:247573/1‑149 (MQ=255)
                                                                                                   ttcgaGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGAGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACa                                                  <  8:161669/146‑1 (MQ=255)
                                                                                                   ttcgaGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGAGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACa                                                  >  7:161669/1‑146 (MQ=255)
                                                                                                       aGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCTCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCTTCTg                                           <  4:158019/149‑1 (MQ=255)
                                                                                                           ccTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCTTCTGgcgc                                       <  3:271828/149‑1 (MQ=255)
                                                                                                                     ttCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCTTCTGGCGCTTGCGTTTgg                             >  2:44866/1‑149 (MQ=255)
                                                                                                                       cgcgGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCTTCTGGCGCTTGCGTTTgg                             <  2:314729/147‑1 (MQ=255)
                                                                                                                        gcgggcggTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCTTCTGGCGCTTGCGTTTGGGtt                          <  7:331130/149‑1 (MQ=255)
                                                                                                                           ggcggTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCTTCTGGCGCTTGCGTTTGGGTTTGa                       <  2:122708/149‑1 (MQ=255)
                                                                                                                           ggcggTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCTTCTGGCGCTTGCGTTTGGGTTTGa                       <  7:216846/149‑1 (MQ=255)
                                                                                                                                tGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCTTCTGGCGCTTGCGTTTGGGTTTGAGCAtt                  >  4:216118/1‑149 (MQ=255)
                                                                                                                                  cccTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCTTCTGGCGCTTGCGTTTGGGTTTGAGCAttt                 <  3:216118/148‑1 (MQ=255)
                                                                                                                                   tgatGGAGCATTTCGAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCTTCTGGCGCTTGCGTTTGGGTTTGAGCATTTCg               >  2:179025/4‑149 (MQ=255)
                                                                                                                                       ggAGCATTTCGAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCTTCTGGCGCTTGCGTTTGGGTTTGAGCAttt                 <  8:120012/143‑1 (MQ=255)
                                                                                                                                       ggAGCATTTCGAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCTTCTGGCGCTTGCGTTTGGGTTTGAGCAttt                 >  7:120012/1‑143 (MQ=255)
                                                                                                                                             ttCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTTACTGATTCGACGATCACATCGGACAGCTTCTGGCGCTTGCGTTTGGGTTTGAGCATTTCGCTATTTTTCa     <  3:142261/149‑1 (MQ=255)
                                                                                                                                             ttCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTCACTGATTCGACGATCACATCGGACAGCTTCTGGCGCTTGCGTTTGGGTTTGAGCATTTCGCTATTTTTCa     >  7:244464/1‑149 (MQ=255)
                                                                                                                                              tCGTAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCTTCTGGCGCTTGCGTTTGGGTTTGAGCATTTCGCTATTTTTCa     >  6:248160/1‑148 (MQ=255)
                                                                                                                                                gAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGa                                                    >  6:264732/1‑99 (MQ=255)
                                                                                                                                                gAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGa                                                    <  5:264732/99‑1 (MQ=255)
                                                                                                                                                gAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCTTCTGGCGCTTGCGTTTGGGTTTGAGCATTTCGCTATTTTTCatat  >  5:373824/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CAGGTAGTTGCGCAGTGCGCGGCTGGCTGGTTCGGTGCCCACTTCGTTCAGGTAGGCGCCCCCGGTCGGGCCGGTGCGGGTGCTCACCAGGCCTTCGACTTCGAGCGCCTTGAGGGCTTCGCGGGCGGTGCCCTTGGAGCATTCGAAATGCTCCATCAGCTCCCGCTCGGTAGGCAGGCGGTCGCCGGGGTGCAGCGCTTCGGTGACGATCGAGCGCTTGACTGATTCGACGATCACATCGGACAGCTTCTGGCGCTTGCGTTTGGGTTTGAGCATTTCGCTATTTTTCATAT  >  NC_002947/726478‑726770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑