Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	848,503	G→A	5.8%	L23F (CTT→TTT)	PP_0730 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	848,503	0	G	A	5.8%	204.0 / 2.2	69	L23F (CTT→TTT)	PP_0730	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (32/33);  new base A (2/2);  total (34/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.97e-01

GGCTGAATTCGCCGCCTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGAAGCAAGCGACCGTCGAAGGT  >  NC_002947/848356‑848648                                                                                                                                                    |                                                                                                                                                  ggCTGAATTCGCCGCCTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGc                                                                                                                                                  >  5:35912/1‑149 (MQ=255)  gCTGAATTCGCCGCCTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGcc                                                                                                                                                 >  7:65444/1‑149 (MQ=255)  gCTGAATTCGCCGCCTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGcc                                                                                                                                                 >  5:191948/1‑149 (MQ=255)     gAATTCGCCGCCTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGtt                                                                                                                                              >  7:302686/1‑149 (MQ=255)           gccgccTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCt                                                                                                                                         <  2:343327/148‑1 (MQ=255)           gccgccTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCt                                                                                                                                         <  2:145421/148‑1 (MQ=255)           gccgccTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTg                                                                                                                                        <  4:4533/149‑1 (MQ=255)            ccgccTTCCAGATTTTAGATACGCAAATAAGCATCCAAATATCGCAGGTAAGGCTCGTGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGc                                                                                                                                       <  7:307107/149‑1 (MQ=255)            ccgccTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGc                                                                                                                                       <  5:145989/149‑1 (MQ=255)             cgccTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGcc                                                                                                                                      <  2:143509/149‑1 (MQ=255)             cgccTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAACGGCTTGGCTGcc                                                                                                                                      <  6:308414/149‑1 (MQ=255)                cTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGc                                                                                                                                       <  7:259632/145‑1 (MQ=255)                cTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGc                                                                                                                                       >  8:259632/1‑145 (MQ=255)                 ttCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCa                                                                                                                                   >  3:47520/1‑148 (MQ=255)                   ccAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCaa                                                                                                                                  <  8:302686/147‑1 (MQ=255)                       aTTATCGATACGAAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTTATCGAGATCTTCAGGAACTAGGCGTCGCTCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCGAAAAAGc                                                                                                                              >  4:183885/1‑147 (MQ=255)                             gATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGc                                                                                                                      >  1:155939/1‑149 (MQ=255)                                 cGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCt                                                                                                                  >  1:4912/1‑149 (MQ=255)                                     aaTAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACg                                                                                                              <  3:110770/149‑1 (MQ=255)                                     aaTAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACg                                                                                                              <  2:155939/149‑1 (MQ=255)                                      aTAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGAGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACgg                                                                                                             >  5:285281/1‑149 (MQ=255)                                          gCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCt                                                                                                         >  6:177479/1‑149 (MQ=255)                                            aTCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGt                                                                                                       >  7:264804/1‑149 (MQ=255)                                               cacaTATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATGGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTcga                                                                                                    <  8:65444/149‑1 (MQ=255)                                                acaTATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGt                                                                                                                                               >  8:51006/1‑105 (MQ=255)                                                acaTATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGt                                                                                                                                               <  7:51006/105‑1 (MQ=255)                                                acaTATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTcgac                                                                                                   >  4:196621/1‑149 (MQ=255)                                                          ggTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCTTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACg                                                                                         <  5:104910/149‑1 (MQ=255)                                                                gCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTc                                                                                   <  6:191948/149‑1 (MQ=255)                                                                 cTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCa                                                                                  <  8:311314/149‑1 (MQ=255)                                                                  tCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCAt                                                                                 >  1:193756/1‑149 (MQ=255)                                                                    gagaCGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGCCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCAtt                                                                                >  3:167175/1‑148 (MQ=255)                                                                     agaCGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTg                                                                               >  5:251568/1‑148 (MQ=255)                                                                           gAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTc                                                                        >  7:87316/1‑149 (MQ=255)                                                                            aGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCg                                                                       <  8:262120/149‑1 (MQ=255)                                                                             ggggtcgtcCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCgg                                                                      <  5:48471/146‑1 (MQ=255)                                                                             ggTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTct                                                                       >  5:310034/1‑147 (MQ=255)                                                                                    tcCTTGATCGAGAGCGTCAGGAACGAGGCGTCGCGCTGATGAATGCAGGTGGCGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCa                                                               <  6:371997/149‑1 (MQ=255)                                                                                    tcCTTGATCAAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTAc                                                                 >  8:274482/1‑147 (MQ=255)                                                                                      cTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAgg                                                             <  3:196621/149‑1 (MQ=255)                                                                                         taTCGAGATCGTCAGGAACGATGCGTCGCGCTGATGAATGAAGGTGACGTGGCATCCAAGACGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTTTCGACGAAAACCACTTGTTTCATTGGAACCTCGGATTACCAggg                                                            <  3:183885/146‑1 (MQ=255)                                                                                         gATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTCTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGAt                                                          >  5:32640/1‑149 (MQ=255)                                                                                          aTCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCGCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACCAGTTTCATTGGAACCTCGGCTTACCAGGGAt                                                          >  8:111067/1‑148 (MQ=255)                                                                                           tCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATg                                                         >  4:63718/1‑148 (MQ=255)                                                                                           tCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGa                                                        <  4:47520/149‑1 (MQ=255)                                                                                               gaTCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGt                                                    <  2:4912/149‑1 (MQ=255)                                                                                               gaTCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGt                                                    <  1:150033/149‑1 (MQ=255)                                                                                                aTCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCGAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCATGGATGAAAGt                                                    <  6:35912/148‑1 (MQ=255)                                                                                                    tCAGGAACGAGGCGGCGCGCTGATGAATGAAGGTGACGGGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATa                                               <  8:120545/149‑1 (MQ=255)                                                                                                         aaCGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCt                                          <  7:274482/149‑1 (MQ=255)                                                                                                             aGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGc                                      >  5:137128/1‑149 (MQ=255)                                                                                                                cggcgctCTGATGAATGAAGGTGCAGTGGCAGCCAAGCCGTTTGGCAGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCg                                   <  2:104333/146‑1 (MQ=255)                                                                                                                 gTCGCGCTGATGAATGAAGGTGACGTGGCAGCCACGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCg                                   >  6:20745/1‑148 (MQ=255)                                                                                                                    gcgcTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGGCCGaa                                 >  2:133865/1‑147 (MQ=255)                                                                                                                    gcgcTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACa                               <  1:131010/149‑1 (MQ=255)                                                                                                                    gcgcTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACa                               <  2:193756/149‑1 (MQ=255)                                                                                                                              atgaaGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACGTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGaa                     <  5:177479/149‑1 (MQ=255)                                                                                                                              atgaaGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGaa                     <  8:87316/149‑1 (MQ=255)                                                                                                                               tgaaGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGaag                    >  2:363023/1‑149 (MQ=255)                                                                                                                                   ggTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGaagcaag                <  1:363023/149‑1 (MQ=255)                                                                                                                                   ggTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGaagcaa                 <  3:116912/148‑1 (MQ=255)                                                                                                                                   ggTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGaagc                   >  4:116912/1‑146 (MQ=255)                                                                                                                                     tGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGACCGAACAGCATTGCGAAGCAAGCg              >  3:204742/1‑149 (MQ=255)                                                                                                                                         gTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGAAGCAAGCGACCg          >  6:156551/1‑149 (MQ=255)                                                                                                                                         gTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGAAGCAAGCGACCg          >  8:139279/1‑149 (MQ=255)                                                                                                                                           ggCAGCCAAACGGCTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGAAGCAAGCGACCGTc        >  4:83507/1‑149 (MQ=255)                                                                                                                                            gCAGCCAAACGGCTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTg                                            >  2:254605/1‑112 (MQ=255)                                                                                                                                            gCAGCCAAACGGCTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTg                                            <  1:254605/112‑1 (MQ=255)                                                                                                                                                 cAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGAAGCAAGCGACCGTCGAAGGt  >  6:329394/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                  GGCTGAATTCGCCGCCTTCCAGATTATCGATACGCAAATAAGCATCCACATATCGCAGGTAAGGCTCGAGACGGGAGGTGTCGTCCTTGATCGAGATCGTCAGGAACGAGGCGTCGCGCTGATGAATGAAGGTGACGTGGCAGCCAAGCCGTTTGGCTGCCTCAAAAGCCAGCAGGCCGCTTACGGTGCTGTCGACGAAAACCACGTGTTTCATTGGAACCTCGGATTACCAGGGATGAAAGTAAATAGTGCTGAGCCCGAACAGCATTGCGAAGCAAGCGACCGTCGAAGGT  >  NC_002947/848356‑848648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑