Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	862,835	C→G	6.9%	N296K (AAC→AAG) ‡	yfcH →	NAD(P)‑binding Rossmann‑fold domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	862,835	0	C	G	6.9%	229.3 / 5.8	72	N296K (AAC→AAG) ‡	yfcH	NAD(P)‑binding Rossmann‑fold domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (33/34);  new base G (2/3);  total (35/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01

CGCTGTTGCCAGTGCCGGCATTGCTGCTCAAGGCCGGGCTTGGCGAGATGTCCACCTTGCTGCTGGGAGGCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGTTCCCCGGCCTCCACCTCGGTGGCCGATGTGCGCCGCTACCTCAACCAGTTCCTCATGGACCCGTACGTGATCGACCTGCCG  >  NC_002947/862687‑862983                                                                                                                                                        |                                                                                                                                                     cgcTGTTGCCAGTGCCGGCATTGCTGCTCAAGGCCGGGCTTGGCGAGATGTCCACCTTGCTGCTGGGATGCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAAc                                                                                                                                                      <  2:298978/149‑1 (MQ=255) cgcTGTTGCCAGTGCCGGCATTGCTGCTCAAGGCCGGGCTTGGCGAGATGTCCACCTTGCTGCTGGGAGGCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAAc                                                                                                                                                      <  4:74089/149‑1 (MQ=255) cgcTGTTGCCAGTGCCGGCATTGCTGCTCAAGGCCGGGCTTGGCGAGATGTCCACCTTGCTGCTGGGAGGCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAAc                                                                                                                                                      <  6:74942/149‑1 (MQ=255)    tGTTGCCAGTGCCGGCATTGCTGCTCAAGGCCGGGCTTGGCGAGATGTCCACCTTGCTGCTGGGAGGCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTg                                                                                                                                                   <  7:305894/149‑1 (MQ=255)        gCCAGTGCCGGCATTGCTGCTCAAGGCCGGGCTTGGCGAGATGTCCACCTTGCTGCTGGGAGGCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTc                                                                                                                                                 >  8:290831/1‑147 (MQ=255)         ccAGTGCCGGCATTGCTGCTCAAGGCCGGGCTTGGCGAGATGTCCACCTTGCTGCTGGGAGGCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAg                                                                                                                                              >  4:49605/1‑149 (MQ=255)             tGCCGGCATTGCTGCTCAAGGCCGGGCTTGGCGAGATGTCCACCTTGCTGCTGGGAGGCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGc                                                                                                                                          <  6:326167/149‑1 (MQ=255)             tGCCGGCATTGCTGCTCAAGGCCGGGCTTGGCGAGATGTCCACCTTGCGGCTGGGAGGCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCGACTGGACAGGTTGTCCAgcgc                                                                                                                                           <  6:217359/149‑4 (MQ=255)               ccGGCATTGCTGCTCAAGGCCGGGCTTGGCGAGATGTCCACCTTGCTGCTGGGAGGCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGcc                                                                                                                                         <  3:217008/148‑1 (MQ=255)                cGGCATTGCTGCTCAAGGCCGGGCTTGGCGAGATGTCCACCTTGCTGCTGGGAGGCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCctc                                                                                                                                       >  5:301961/1‑149 (MQ=255)                 ggCATTGCTGCTCAAGGCCGGGCTTGGCGAGATGTCCACCTTGCTGCTGGGAGGCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCctct                                                                                                                                      >  2:1929/1‑149 (MQ=255)                  gCATTGCTGCTCAAGGCCGGGCTTGGCGAGATGTCCACCTTGCTGCTGGGAGGCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTg                                                                                                                                     <  2:288281/149‑1 (MQ=255)                        ctgctCAAGGCCGGGCTTGGCGAGATGTCCACCTTGCTGCTGGGAGGCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGTACAACCTGTCCAGTCGCCTCTGACATa                                                                                                                                <  6:301961/148‑1 (MQ=255)                        ctgctCAAGGCCGGGCTTGGCGAGATGTCCACCTTGCTGCTGGGAGGCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAg                                                                                                                               >  6:154734/1‑149 (MQ=255)                           ctCAAGGCCGGGCTTGGCGAGATGTCCACCTTGCTGCTGGGAGGCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAgg                                                                                                                              <  7:298489/147‑1 (MQ=255)                              aaGGCCGGGTTTGGCGAGATGTCCACCTTGCTGGTGGGAGGCCAGCGGGCGCGCCCGGTTCGCTTGCTGTAAGCCGGTTTCAGCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCTGTCGCCTCTGACATAGGACGCt                                                                                                                         <  8:127509/149‑1 (MQ=255)                              aaGGCCGGGCTTGGCGAGATGTCCACCTTGCTGCGGGGAGGCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCt                                                                                                                         <  2:85313/149‑1 (MQ=255)                                ggCCGGGCTTGGCGAGATGTCCACCTTGCTGCTGGGAGGCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGc                                                                                                                       <  3:49605/149‑1 (MQ=255)                                 gCCGGGCTTGGCGAGATGTCCACCTTGCTGCTGGGAGGCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCa                                                                                                                      >  8:320483/1‑149 (MQ=255)                                                  gTCCACCTCGCTGCTGGGAGGCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCctg                                                                                                     >  4:355483/1‑149 (MQ=255)                                                   tCCACCTTGCTGCTGGGAGGCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCctg                                                                                                     <  7:320483/148‑1 (MQ=255)                                                     cACCTTGCTGCTGGGAGGCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTg                                                                                                                        <  4:263456/127‑1 (MQ=255)                                                     cACCTTGCTGCTGGGAGGCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTg                                                                                                                        >  3:263456/1‑127 (MQ=255)                                                     cACCTTGCTGCTGGGAGGCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCctgctg                                                                                                  >  2:311224/1‑149 (MQ=255)                                                     cACCTTGCTGCTGGGAGGCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCctgctg                                                                                                  >  5:39887/1‑149 (MQ=255)                                                        cTTGCTGCTGGGAGGCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCctgctgctg                                                                                               <  5:58178/149‑1 (MQ=255)                                                        cTTGCTGCTGGGAGGCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCctgctgctg                                                                                               <  1:1929/149‑1 (MQ=255)                                                         ttGCTGCTGGGAGGCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTgg                                                                                              >  2:370877/1‑149 (MQ=255)                                                              gctgGGAGGCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTc                                                                                                                                                 >  4:307923/1‑93 (MQ=255)                                                              gctgGGAGGCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTc                                                                                                                                                 <  3:307923/93‑1 (MQ=255)                                                               ctgGGAGGCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTTGCCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAAc                                                                                         >  1:284563/1‑149 (MQ=255)                                                                 gggAGGCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTg                                                                                      <  4:227/149‑1 (MQ=255)                                                                     ggCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGtt                                                                                  <  7:70395/149‑1 (MQ=255)                                                                     ggCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGtt                                                                                  <  7:65317/149‑1 (MQ=255)                                                                      gCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCctgctgct                                                                                                <  5:150433/134‑1 (MQ=255)                                                                      gCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCctgctgct                                                                                                >  6:150433/1‑134 (MQ=255)                                                                      gCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCctgctgct                                                                                                >  6:144518/1‑134 (MQ=255)                                                                      gCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCctgctgct                                                                                                <  5:144518/134‑1 (MQ=255)                                                                        cAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCCACCTGGGTTccc                                                                               >  4:363633/1‑149 (MQ=255)                                                                        cAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGTTccc                                                                               >  3:124480/1‑149 (MQ=255)                                                                             ggCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGTTCCCCGGcc                                                                          <  2:284563/149‑1 (MQ=255)                                                                                gcggCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCC‑CTGGACAACCTGTCCAGGCGCCTCTGACATAGGCCCCTGCATGACCGCTCACGCCCTGCTGCTGGTCAACCTGGGGTCCCCGGCCTcc                                                                       <  1:709/149‑1 (MQ=255)                                                                                          cGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGTTCTCCGGCCTCCACCTCGGTg                                                              <  7:290831/148‑1 (MQ=255)                                                                                          cGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGTTCCCCGGCCTCCACCTCGGTg                                                              <  4:208120/148‑1 (MQ=255)                                                                                             ttGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAATGCGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGTTCCCCGGCCTCCACCTCGGTGGcc                                                           >  1:71168/1‑149 (MQ=255)                                                                                                cTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGTTCCCCGGCCTc                                                                        >  6:5497/1‑132 (MQ=255)                                                                                                cTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGTTCCCCGGCCTc                                                                        <  5:5497/132‑1 (MQ=255)                                                                                                cTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGTTCCCCGGCCTCCACCTCGGTGGCCGAtg                                                       <  4:124480/149‑1 (MQ=255)                                                                                                 tGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGAc                                                                                                                  <  4:95068/89‑1 (MQ=255)                                                                                                 tGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGTTCCCCGGCCTCCACCTCGGTGGCCGAtgt                                                      >  7:81474/1‑149 (MQ=255)                                                                                                         ggCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCt                                                                                       <  4:262242/108‑1 (MQ=255)                                                                                                         ggCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCt                                                                                       >  3:262242/1‑108 (MQ=255)                                                                                                           cTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGTTCCCCGGCCTCCACCTCGGTGGCCGATGTGCGCCGCTAc                                            <  3:355483/149‑1 (MQ=255)                                                                                                            ttCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGTTCCCCGGCCTCCACCTCGGTGGCCGATGTGCGCCGCTAcc                                           >  1:167534/1‑149 (MQ=255)                                                                                                               aCCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGTTCCCCGGCCTCCACCTCGGTGGCCGATGTGCGCCGCTACCTc                                         >  6:54007/1‑148 (MQ=255)                                                                                                                 cTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGTTCCCCGGCCTCCACCTCGGTGGCCGATGTGCGCCGCTACCTCAAc                                      >  8:323982/1‑149 (MQ=255)                                                                                                                       gCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGTTCCCCGGCCTCCACCTCGGTGGCCGATGTGCGCCGCTACCTCAACCAGtt                                 >  8:407/1‑149 (MQ=255)                                                                                                                            aaCGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGTTCCCCGGCCTCCACCTCGGTGGCCGATGTGCGCCGCTACCTCAACCAGTTCCTc                             <  2:71168/148‑1 (MQ=255)                                                                                                                             aCGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGTTCCCCGGCCTCCACCTCGGTGGCCGATGTGCGCCGCTACCTCAACCAGTTCCTCAt                           <  2:167534/149‑1 (MQ=255)                                                                                                                             aCGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGTTCCCCGGCCTCCACCTCGGTGGCCGATGTGCGCCGCTACCTCAACCAGTTCCTCAt                           <  1:370877/149‑1 (MQ=255)                                                                                                                              cGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATTGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGTTCCCCGGCCTCCACCTCGGTGGCCGATGTGCGCCGCTACCTCAACCAGTTCCTCATg                          <  1:311224/149‑1 (MQ=255)                                                                                                                               gATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGTTCCCCGGCCTCCACCTCGGTGGCCGATGTGCGCCGCTACCTCAACCAGTTCCTCATgg                         >  7:249722/1‑149 (MQ=255)                                                                                                                                   tGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCACCCTGGGTTCCCCGGCCTCCACCTCGGTGGCCGAGGTCCGCCGCGACCTCAACCAGATCCTCATggcc                       >  8:260747/1‑145 (MQ=255)                                                                                                                                   tGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGTTCCCCGGCCTCCACCTCGGTGGCCGATGTGCGCCGCTACCTCAACCAGTTCCTCATGGAccc                     >  5:183760/1‑149 (MQ=255)                                                                                                                                   tGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGTTCCCCGGCCTCCACCTCGGTGGCCGATGTGCGCCGCTACCTCAACCAGTTCCTCATGGAccc                     >  2:16983/1‑149 (MQ=255)                                                                                                                                   tGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGTTCCCCGGCCTCCACCTCGGTGGCCGATGTGCGCCGCTACCTCAACCAGTTCCTCATGGAccc                     >  1:330525/1‑149 (MQ=255)                                                                                                                                    gCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGTTCCCCGGCCTCCACCTCGGTGGCCGATGTGCGCCGCTACCTCAACCAGTTCCTCATGGACCCg                    >  8:5223/1‑149 (MQ=255)                                                                                                                                     cAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGTTCCCCGGCCTCCACCTCGGTGGCCGATGTGCGCCGCTACCTCAACCAGTTCCTCATGGAccc                     <  6:39887/147‑1 (MQ=255)                                                                                                                                     cAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGTTCCCCGGCCTCCACCTCGGTGGCCGATGTGCGCCGCTACCTCAACCAGTTCCTCATGGAc                       <  8:249722/145‑1 (MQ=255)                                                                                                                                             cgacTGGACAGGTTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGTTCCCCGGCCTCCACCTCGGTGGCCGATGTGCGCCGCTACCTCAACCAGTTCCTCATGGACCCGTACGTGa             >  5:187056/4‑149 (MQ=255)                                                                                                                                              gacTGGACAGGTTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGTTCCCCGGCCTCCACCTCGGTGGCCGATGTGCGCCGCTACCTCAACCAGTTCCTCATGGa                        <  2:189539/135‑1 (MQ=255)                                                                                                                                              gacTGGACAGGTTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGTTCCCCGGCCTCCACCTCGGTGGCCGATGTGCGCCGCTACCTCAACCAGTTCCTCATGGa                        >  1:189539/3‑137 (MQ=255)                                                                                                                                               acTGGACAGGTTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGTTCCCCGGCCTCCACCTCGGTGGCCGATGTGCGCCGCTACCTCAACCAGTTCCTCATGGACCCGTACGTGATc           <  6:187056/148‑1 (MQ=255)                                                                                                                                                     cAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGTTCCCCGGCCTCCACCTCGGTGGCCGATGTGCGCCGCTACCTCAACCAGTTCCTCATGGACCCGTACGTGATCGACCTg     >  2:15456/1‑149 (MQ=255)                                                                                                                                                      aaCCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGTTCCCCGGCCTCCACCTCGGTGGCCGATGTGCGCCGCTACCTCAACCAGTTCCTCATGGACCCGTACGTGATCGACCTGc    >  3:248426/1‑149 (MQ=255)                                                                                                                                                        ccTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGTTCCCCGGCCTCCACCTCGGTGGCCGATGTGCGCCGCTACCTCAACCAGTTCCTCATGGACCCGTACGTGATCGACCTGCCg  >  3:226243/1‑149 (MQ=255)                                                                                                                                                        |                                                                                                                                                     CGCTGTTGCCAGTGCCGGCATTGCTGCTCAAGGCCGGGCTTGGCGAGATGTCCACCTTGCTGCTGGGAGGCCAGCGGGCGCGCCCGGTACGCTTGCTGGAAGCCGGCTTCACCTT‑CCGCTTCAACGATCTGCAAT‑CGGCG‑CTGGACAACCTGTCCAGTCGCCTCTGACATAGGACGCTGCATGACCGATCACGCCCTGCTGCTGGTCAACCTGGGTTCCCCGGCCTCCACCTCGGTGGCCGATGTGCGCCGCTACCTCAACCAGTTCCTCATGGACCCGTACGTGATCGACCTGCCG  >  NC_002947/862687‑862983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑