Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	873,425	T→C	7.6%	L20P (CTT→CCT)	PP_5457 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	873,425	0	T	C	7.6%	231.7 / 6.7	80	L20P (CTT→CCT)	PP_5457	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (38/35);  new base C (3/3);  total (41/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCAGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGCGTACAATGATGCC  >  NC_002947/873279‑873573                                                                                                                                                   |                                                                                                                                                      gCCAGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCtt                                                                                                                                                      <  3:160046/148‑1 (MQ=255) gCCAGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCtt                                                                                                                                                      <  3:241319/148‑1 (MQ=255)  ccAGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGc                                                                                                                                                    >  7:148801/1‑149 (MQ=255)    aGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGCTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGAATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAAAGCTGGCAGGCATGCTTCCCa                                                                                                                                                  >  3:326467/1‑149 (MQ=255)      gtggGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAAGCATGCCTGCCAGc                                                                                                                                                <  6:201179/149‑1 (MQ=255)         tgCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAg                                                                                                                                             >  4:63499/1‑149 (MQ=255)           cTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAggg                                                                                                                                           >  4:140604/1‑149 (MQ=255)           cTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCAGCGAGGACGACACCTGATGCAGCGTATGTACGAACCGGATAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAgg                                                                                                                                            <  8:15436/148‑1 (MQ=255)             gTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGa                                                                                                                                         >  4:368413/1‑149 (MQ=255)                aGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATc                                                                                                                                       >  2:103981/1‑148 (MQ=255)                 gCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGa                                                                                                                                     >  5:227074/1‑149 (MQ=255)                  gcTGTGGTGTGCATTCTGCAGGTGCATTGTTAGGATTGGTTGCTTGAAGTCCTGCGGGAGGTCGACTCCTGATGGTGGGTATCTACGAACCGGAAAGCGTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGAt                                                                                                                                    <  4:326467/148‑1 (MQ=255)                  ccTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGAt                                                                                                                                    <  8:148801/149‑1 (MQ=255)                     gtggtgTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGt                                                                                                                                  >  3:147030/1‑148 (MQ=255)                        gtgtGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTacac                                                                                                                              >  6:194689/1‑149 (MQ=255)                        gtgtGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTacac                                                                                                                              >  8:37355/1‑149 (MQ=255)                          gtgCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGt                                                                                                                            >  2:274561/1‑149 (MQ=255)                           tgCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGt                                                                                                                            <  3:140604/148‑1 (MQ=255)                           tgCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTg                                                                                                                           >  1:374041/1‑149 (MQ=255)                              aTTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTc                                                                                                                        >  1:233327/1‑149 (MQ=255)                                  tGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGcc                                                                                                                    >  7:117588/1‑149 (MQ=255)                                    cAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGcc                                                                                                                    <  5:15498/147‑1 (MQ=255)                                    cAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGcc                                                                                                                    <  2:360978/147‑1 (MQ=255)                                    cAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGt                                                                                                                  <  1:103981/149‑1 (MQ=255)                                    cAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCCGGCAGGCATGCTTGCCAGCGa                                                                                                                                              >  4:98529/1‑121 (MQ=255)                                        tGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGAcc                                                                                                              <  2:374041/149‑1 (MQ=255)                                         gCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCt                                                                                                             >  1:20033/1‑149 (MQ=255)                                            tGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑t                                                                                                         >  3:295240/1‑149 (MQ=255)                                             ggTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGCACACGTGGTCGGCCGTGACCTTG‑t                                                                                                         <  8:260962/148‑1 (MQ=255)                                             ggTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGCACACGTGGTCGGCCGTGACCTTG‑t                                                                                                         >  7:260962/1‑148 (MQ=255)                                              gTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGGCGGCCGTGACCTTG‑Tc                                                                                                        <  1:358463/148‑1 (MQ=255)                                                gACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGt                                                                                                     >  4:169632/1‑149 (MQ=255)                                                 aCGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTg                                                                                                    <  2:233327/149‑1 (MQ=255)                                                   gAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCg                                                                                                       >  4:57290/1‑144 (MQ=255)                                                   gAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCCTGACCTTG‑TCg                                                                                                       <  3:57290/144‑1 (MQ=255)                                                    aaTGGATGCTTGAAGTCCGCCGCGAGGACGTCACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTgccg                                                                                                 >  1:33750/1‑149 (MQ=255)                                                        gATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCgg                                                                                             <  6:266493/149‑1 (MQ=255)                                                         aTGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGc                                                                                            >  8:339028/1‑149 (MQ=255)                                                         aTGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGc                                                                                            >  6:74298/1‑149 (MQ=255)                                                            cTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGa                                                                                          <  3:323735/148‑1 (MQ=255)                                                            cTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAg                                                                                         <  3:368413/149‑1 (MQ=255)                                                                aaGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGc                                                                                     >  5:329376/1‑149 (MQ=255)                                                                  gTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGccc                                                                                   >  3:31933/1‑149 (MQ=255)                                                                  gTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGccc                                                                                   <  3:63499/149‑1 (MQ=255)                                                                  gTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGAGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGccc                                                                                   <  2:166979/149‑1 (MQ=255)                                                                    cagctgagAGGACTAGACATGATGCATCTTATCTAAGAAACGGACAGACTGATTGATGAGCAAATGCTGGCAGGCATGCTTGCATGAGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGACCt                                                                                  <  2:256034/141‑1 (MQ=255)                                                                      gccgcGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGc                                                                               <  6:227074/149‑1 (MQ=255)                                                                           gAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGc                                                                          <  2:20033/149‑1 (MQ=255)                                                                                  acacCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTgg                                                                   <  7:339028/149‑1 (MQ=255)                                                                                  acacCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTgg                                                                   <  4:295240/149‑1 (MQ=255)                                                                                      cTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCt                                                               <  4:147030/149‑1 (MQ=255)                                                                                      cTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCt                                                               >  6:352991/1‑149 (MQ=255)                                                                                      cTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCAAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCt                                                               >  3:354491/1‑149 (MQ=255)                                                                                       tGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCtc                                                              >  8:3201/1‑149 (MQ=255)                                                                                         aTGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCtcgt                                                            >  1:272356/1‑149 (MQ=255)                                                                                            cAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTGCTCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCtcgtcg                                                          <  1:274561/149‑1 (MQ=255)                                                                                            cAGCGTAGCTACGAACCGGAAAGCCTGCGTCTGGCGCAAATGCTGGCAGGCATGCGTGCCAGCGCGGGGCTCGAGGTACACGTGGTCGGCCGTGACCTTT‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTcgtcgt                                                         <  7:270456/149‑1 (MQ=255)                                                                                              gCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTgg                                                       <  7:37355/149‑1 (MQ=255)                                                                                                 tATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCtg                                                    <  4:31933/149‑1 (MQ=255)                                                                                                     tACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCtgat                                                  >  1:173842/1‑147 (MQ=255)                                                                                                       cGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCtgat                                                  <  2:33750/145‑1 (MQ=255)                                                                                                       cGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAg                                               >  2:364118/1‑148 (MQ=255)                                                                                                        gAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAg                                               >  7:87076/1‑147 (MQ=255)                                                                                                            cGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGcc                                         <  1:364118/149‑1 (MQ=255)                                                                                                                   ccTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATgcg                                  >  5:53434/1‑149 (MQ=255)                                                                                                                   ccTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATgcg                                  <  5:194689/149‑1 (MQ=255)                                                                                                                          gAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCAGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACgg                             >  8:283628/1‑147 (MQ=255)                                                                                                                           aGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCa                           <  7:315384/148‑1 (MQ=255)                                                                                                                             gcgcAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCt                        >  7:196423/1‑149 (MQ=255)                                                                                                                               gcAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGa                      >  7:22801/1‑149 (MQ=255)                                                                                                                                 cctcgatGGCAAGCATGCCTGCAAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGACGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGAACGGAACCTGATc                    >  6:44333/7‑149 (MQ=255)                                                                                                                                 aaaTGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATc                    >  6:118707/1‑149 (MQ=255)                                                                                                                                   tcgCTGGCAAGCATGCCTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATc                    >  3:330656/3‑147 (MQ=255)                                                                                                                                   tcgCTGGCAAGCATGCCTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATc                    <  4:330656/145‑1 (MQ=255)                                                                                                                                    tGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGAt                 <  8:117588/149‑1 (MQ=255)                                                                                                                                    cgCTGGCAAGCATGCCTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGAt                 >  8:55062/2‑149 (MQ=255)                                                                                                                                      cTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATg                <  5:352991/148‑1 (MQ=255)                                                                                                                                      cTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGc               <  4:219026/149‑1 (MQ=255)                                                                                                                                         gcaggcaTGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGCGt             <  5:74298/148‑1 (MQ=255)                                                                                                                                          caggcaTGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGCGTAc           <  8:87076/149‑1 (MQ=255)                                                                                                                                          caagcaTGCCTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGCGTAc           <  7:55062/146‑1 (MQ=255)                                                                                                                                                 gCTTGCCAGCGAGGGGATCGATGTACGCGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGCGTACAatgatg    >  1:11175/1‑149 (MQ=255)                                                                                                                                                   ttGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGCGTACAATGATGcc  >  6:311321/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                      GCCAGGTGTGCTGTCAGCCTGTGGTGTGCATTCTGCAGGTGCATGGTGACGAATGGATGCTTGAAGTCCGCCGCGAGGACGACACCTGATGCAGCGTATCTACGAACCGGAAAGCCTGCTTGAGGCGCAAATGCTGGCAGGCATGCTTGCCAGCGAGGGGATCGATGTACACGTGGTCGGCCGTGACCTTG‑TCGGTGCCGCCGGCGAGCTGCCCTTGCAGGGCCTGCTGGGGCTCGTCGTGGCTGATGAGCAGGCCGAATGCGCACGGCAGCTGATCGATGCGTACAATGATGCC  >  NC_002947/873279‑873573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑