Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	100,116	T→G	8.5%	N98T (AAC→ACC) ‡	PP_0094 ←	5'‑nucleotidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	100,116	0	T	G	8.5%	232.4 / 12.0	82	N98T (AAC→ACC) ‡	PP_0094	5'‑nucleotidase
Reads supporting (aligned to +/- strand):  ref base T (37/38);  new base G (5/2);  total (42/40)
Fisher's exact test for biased strand distribution p-value = 4.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.61e-01

TCAGCTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTGCAGCAGGG  >  NC_002947/99970‑100263                                                                                                                                                   |                                                                                                                                                    tCAGCTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGtt                                                                                                                                                    >  1:255894/1‑148 (MQ=255)    gCTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAgg                                                                                                                                                >  6:326758/1‑149 (MQ=255)    gCTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAgg                                                                                                                                                >  1:3830/1‑149 (MQ=255)     cTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAggg                                                                                                                                               <  2:255894/149‑1 (MQ=255)     cTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAggg                                                                                                                                               >  4:263188/1‑149 (MQ=255)           cTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTATGGAGGTGGCAATGTACAGCGTGCGGCCCTGCACGTTCAGGGCCTc                                                                                                                                           >  8:274840/1‑147 (MQ=255)              gttgGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTTCCCGTTCAGGGCCTCAAGc                                                                                                                                       <  5:157401/148‑1 (MQ=255)              gttgGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGc                                                                                                                                       <  5:326758/148‑1 (MQ=255)               gtgGTGCGCGTGCCTTCCCGGTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGGGGCAATGTACAGCGTGCGGCCCTGAACGGGCAGGGCCGCACGct                                                                                                                                      <  6:98031/147‑2 (MQ=255)                  gTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGcc                                                                                                                                             >  6:352771/1‑138 (MQ=255)                  gTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGcc                                                                                                                                             <  5:352771/138‑1 (MQ=255)                  gTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTc                                                                                                                                  >  3:154660/1‑149 (MQ=255)                  gTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTc                                                                                                                                  >  3:149008/1‑149 (MQ=255)                  gTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTc                                                                                                                                  <  8:249484/149‑1 (MQ=255)                      gcgGGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGc                                                                                                                              <  3:42588/149‑1 (MQ=255)                         tGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGcaca                                                                                                                           >  8:97654/1‑149 (MQ=255)                            cGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGAACGGGCAGGGccgc                                                                                                                                           <  3:115309/130‑3 (MQ=255)                            cGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGAACGGGCAGGGccgc                                                                                                                                           >  4:115309/1‑128 (MQ=255)                             gTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCAGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCTTGCCTCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGCCACACCta                                                                                                                       >  4:49363/1‑148 (MQ=255)                                     cACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAacac                                                                                                                >  5:92824/1‑148 (MQ=255)                                      aCTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAacac                                                                                                                >  5:120642/1‑147 (MQ=255)                                      aCTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGa                                                                                                              <  6:62262/149‑1 (MQ=255)                                      aCTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGa                                                                                                              <  3:247849/149‑1 (MQ=255)                                      aCTGCCGTAGATCACCTAGTAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGa                                                                                                              <  8:333260/149‑1 (MQ=255)                                        tGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAgg                                                                                                            >  6:126397/1‑149 (MQ=255)                                          ccGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAgg                                                                                                            <  7:97654/147‑1 (MQ=255)                                               gATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGAGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCATGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTc                                                                                                       <  1:314171/147‑1 (MQ=255)                                               gATCACCTTGAAGTGATGATCGAACGCAAAGTGCAGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTAAGGGCCTCAAGCAACTAAGGCACACCTTCGAACACGAGGtttta                                                                                                       >  2:314171/1‑146 (MQ=255)                                                 tCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGGCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTa                                                                                                    <  1:42183/148‑1 (MQ=255)                                                  cACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTa                                                                                                    >  1:13531/1‑147 (MQ=255)                                                     cTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGAc                                                                                               >  4:199525/1‑149 (MQ=255)                                                     cTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGAc                                                                                               >  5:36865/1‑149 (MQ=255)                                                      ttGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTAAAGCGTGCGGCCCTGCCCCTTCAGGGCCTAACGCAACTCCGGCACACCTTCGAGCACGAGGTTTTCGTAAAGAcc                                                                                              >  5:278931/1‑149 (MQ=255)                                                        gAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCg                                                                                             <  2:3830/148‑1 (MQ=255)                                                            tgatgaTCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCAc                                                                                        >  5:56488/1‑149 (MQ=255)                                                                  tCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGaa                                                                                  <  2:208446/149‑1 (MQ=255)                                                                  tCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAAGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCgg                                                                                    >  2:195615/1‑147 (MQ=255)                                                                   cGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAg                                                                                 >  7:93216/1‑149 (MQ=255)                                                                       cGCTAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCt                                                                             <  1:288181/149‑1 (MQ=255)                                                                            aGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCgg                                                                        >  5:180789/1‑149 (MQ=255)                                                                                  gAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAg                                                                  >  8:339219/1‑149 (MQ=255)                                                                                        tttCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCAcc                                                            <  3:263188/149‑1 (MQ=255)                                                                                            gcgGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCAt                                                        <  6:56488/149‑1 (MQ=255)                                                                                                gCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATcc                                                      >  2:278527/1‑147 (MQ=255)                                                                                                 cGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAg                                                   <  7:339219/149‑1 (MQ=255)                                                                                                     cTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCt                                               >  4:140696/1‑149 (MQ=255)                                                                                                       cccATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTg                                             >  6:69683/1‑149 (MQ=255)                                                                                                        ccATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCtt                                              <  5:126397/147‑1 (MQ=255)                                                                                                        ccATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTgg                                            >  3:115274/1‑149 (MQ=255)                                                                                                        ccATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTgg                                            >  7:89821/1‑149 (MQ=255)                                                                                                        ccATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTgg                                            >  8:134014/1‑149 (MQ=255)                                                                                                          aTGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATACCAGGCCTTGGCt                                          >  6:305296/1‑149 (MQ=255)                                                                                                           tGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCtt                                         <  6:36865/149‑1 (MQ=255)                                                                                                              tttGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTtcgt                                      <  6:92824/149‑1 (MQ=255)                                                                                                              tttGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTtcgt                                      <  4:154660/149‑1 (MQ=255)                                                                                                              tttGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTtcgt                                      <  4:149008/149‑1 (MQ=255)                                                                                                              tttGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTtcgt                                      <  2:13531/149‑1 (MQ=255)                                                                                                                  gAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTtcgtcg                                    >  2:337210/1‑147 (MQ=255)                                                                                                                  gAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGaa                                  <  1:278527/149‑1 (MQ=255)                                                                                                                   aGGTGGCAATGTACAGCGTGCGGCCCTGCGCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAg                                 <  7:274840/149‑1 (MQ=255)                                                                                                                   aGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCTTAAAGACCGGTCACTCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAg                                 <  4:115274/149‑1 (MQ=255)                                                                                                                   aGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAg                                 <  1:147105/149‑1 (MQ=255)                                                                                                                          aaTGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAg                           >  5:63019/1‑148 (MQ=255)                                                                                                                              tACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTtcgtcg                                    <  4:113727/135‑1 (MQ=255)                                                                                                                              tACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTtcgtcg                                    >  3:113727/1‑135 (MQ=255)                                                                                                                                cAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCa                    <  6:180789/149‑1 (MQ=255)                                                                                                                                cAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCa                    <  8:93216/149‑1 (MQ=255)                                                                                                                                  gcttgaggCCCTGAACGGGCAGGGCCGCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCAt                   >  6:79001/7‑148 (MQ=255)                                                                                                                                   cttgaggCCCTGAACGGGCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATg                  >  3:5212/6‑148 (MQ=255)                                                                                                                                    ttgaggCCCTGAACGGGCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGa                 >  7:193297/5‑148 (MQ=255)                                                                                                                                    ttgaggCCCTGAACGGGCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGa                 >  5:14867/5‑148 (MQ=255)                                                                                                                                     tGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGaaa               <  3:199525/149‑1 (MQ=255)                                                                                                                                       cGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGc             <  6:120642/149‑1 (MQ=255)                                                                                                                                       cGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGc             <  1:337210/149‑1 (MQ=255)                                                                                                                                        ttgggtGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGc             <  3:344938/143‑1 (MQ=255)                                                                                                                                        ggCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTCCATGAAAGcc            >  5:2379/1‑149 (MQ=255)                                                                                                                                           ccTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTgc         <  5:69683/149‑1 (MQ=255)                                                                                                                                            cTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTg          >  3:254378/1‑147 (MQ=255)                                                                                                                                                ccGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTGcagcag    >  7:224195/1‑149 (MQ=255)                                                                                                                                                 cGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTGCAGCAgg   <  3:140696/149‑1 (MQ=255)                                                                                                                                                 cGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTGCAGCAgg   >  8:4762/1‑149 (MQ=255)                                                                                                                                                 cGTTCAAGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTGCAGCAgg   <  1:195615/149‑1 (MQ=255)                                                                                                                                                  gTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACAGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTGCAGCAggg  >  5:142918/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                    TCAGCTCGACCTTGTTGGTGCGCGTGCCGTCCAGTTCACTGCCGTAGATCACCTTGAAGTGATGATCGAACGCAAAGTGCCGAGCGATTTCGCGGGCGAACTCCCATGGTTTGGAGGTGGCAATGTACAGCGTGCGGCCCTGCCCGTTCAGGGCCTCAAGCAACTCCGGCACACCTTCGAACACGAGGTTTTCGTAAAGACCGGTCACGCGGAAGCGCTCGCGGTAGAAGTTCACCGCATCCCAGGCCTTGGCTTCGTCGAAGCTGTAGAACTGCATGAAAGCCTGCAGCAGGG  >  NC_002947/99970‑100263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑