Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 902,620 A→T 8.8% intergenic (+46/+30) trxB → / ← nadC thioredoxin reductase/quinolinate phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947902,6200AT 8.8% 202.2 / 6.1 78intergenic (+46/+30)trxB/nadCthioredoxin reductase/quinolinate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (33/38);  new base T (4/3);  total (37/41)
Fisher's exact test for biased strand distribution p-value = 7.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

ACCACGTTTACCGCCAGGCCATCACTTCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATGTAGTCCACCCCCGTCTCGGCAATCACCCGCAGGGTCGTTTCGTTGAC  >  NC_002947/902474‑902763
                                                                                                                                                  |                                                                                                                                               
aCCACGTTTACCGCCAGGCCATCACTTCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCaa                                                                                                                                                 <  5:10878/147‑1 (MQ=255)
 ccACGTTTACCGCCAGGCCATCACTTCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAgcg                                                                                                                                              >  4:306334/1‑149 (MQ=255)
 ccACGTTTACCGCCAGGCCATCACTTCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAgcg                                                                                                                                              >  5:37840/1‑149 (MQ=255)
          ccGCCAGGCCATCACTTCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGttttt                                                                                                                                     <  7:153811/149‑1 (MQ=255)
           cGCCAGGCCATCACTTCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGtttttt                                                                                                                                    >  2:184/1‑149 (MQ=255)
            gCCAGGCCATCACTTCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCGCTTGGCCGGTTTTTTa                                                                                                                                   <  6:149087/149‑1 (MQ=255)
            gCCAGGCCATCACTTCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTa                                                                                                                                   <  3:337657/149‑1 (MQ=255)
            gCCAGGCCATCACTTCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTa                                                                                                                                   >  4:16493/1‑149 (MQ=255)
             ccAGGCCATCACTTCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTAt                                                                                                                                  >  4:235927/1‑149 (MQ=255)
              cAGGCCATCACTTCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTAtt                                                                                                                                 <  7:315099/149‑1 (MQ=255)
              cAGGCCATCACTTCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTAtt                                                                                                                                 >  2:243/1‑149 (MQ=255)
              cAGGCCATCACTTCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGGCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTAtt                                                                                                                                 <  6:232106/149‑1 (MQ=255)
                 gCCATCACTTCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGcc                                                                                                                              >  6:61727/1‑149 (MQ=255)
                 gCCATCACTTCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGcc                                                                                                                              <  2:309265/149‑1 (MQ=255)
                  ccATCACTTCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCGCTTGGCCGGTTTTTTATTgggg                                                                                                                             <  1:317246/149‑4 (MQ=39)
                    aTCACTTCGGCCGGTGCGGCCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTg                                                                                                                            >  2:184685/1‑148 (MQ=255)
                       aCTTCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTGGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGc                                                                                                                               <  2:53220/142‑1 (MQ=255)
                       aCTTCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGc                                                                                                                               >  1:53220/1‑142 (MQ=255)
                       aCTTCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAAt                                                                                                                        <  5:23088/149‑1 (MQ=255)
                         ttCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAAt                                                                                                                        >  1:167708/1‑147 (MQ=255)
                             gCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCt                                                                                                                   >  2:172139/1‑148 (MQ=255)
                               cGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTcac                                                                                                                <  3:306334/149‑1 (MQ=255)
                               cGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTcac                                                                                                                <  1:184/149‑1 (MQ=255)
                                        cTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGt                                                                                                       <  3:235927/149‑1 (MQ=255)
                                           cATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCGCTTGGCCGGTTTTTTAt                                                                                                                                  <  5:158486/119‑1 (MQ=38)
                                           cATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCGCTTGGCCGGTTTTTTAt                                                                                                                                  >  6:158486/1‑119 (MQ=38)
                                            aTGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCa                                                                                                   >  4:4756/1‑149 (MQ=255)
                                            aTGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCa                                                                                                   <  5:61727/149‑1 (MQ=255)
                                             tGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCAt                                                                                                  >  6:378993/1‑149 (MQ=255)
                                                cggcgCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGa                                                                                               <  1:371411/149‑1 (MQ=255)
                                                 ggcgCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGAc                                                                                              <  3:176937/149‑1 (MQ=255)
                                                  gcgcTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACa                                                                                             <  1:31050/149‑1 (MQ=255)
                                                   cgcTGGATGTCGAGCGCTACCTGGACGGTCTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAg                                                                                            <  5:333394/149‑1 (MQ=255)
                                                         aTGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCAc                                                                                      >  2:96352/1‑149 (MQ=255)
                                                          tGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCAcc                                                                                     >  7:292787/1‑149 (MQ=255)
                                                                gcgcTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCTCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTc                                                                               <  8:228732/149‑1 (MQ=255)
                                                                gcgcTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTc                                                                               <  6:287502/149‑1 (MQ=255)
                                                                      ccTGGACGGTTTGGTCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCAt                                                                                                  <  5:208978/124‑1 (MQ=255)
                                                                      ccTGGACGGTTTGGTCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCAt                                                                                                  >  6:208978/1‑124 (MQ=255)
                                                                          gACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTGGCATCGACAGGTCCACCGCTTTCACATCCTTg                                                                      <  1:234557/148‑1 (MQ=255)
                                                                          gACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCtt                                                                       <  7:332541/147‑1 (MQ=255)
                                                                          gACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTg                                                                      <  1:184685/148‑1 (MQ=255)
                                                                            cGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTc                                                                   <  7:121062/149‑1 (MQ=255)
                                                                               tttGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATg                                                                <  6:38577/149‑1 (MQ=255)
                                                                               tttGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATg                                                                <  3:16493/149‑1 (MQ=255)
                                                                                        cGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATc                                                       <  7:238200/149‑1 (MQ=255)
                                                                                         gCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATc                                                       >  6:327715/1‑148 (MQ=255)
                                                                                         gCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCg                                                      <  3:4756/149‑1 (MQ=255)
                                                                                          ccTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCga                                                     >  2:258762/1‑149 (MQ=255)
                                                                                          ccTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCga                                                     <  2:167708/149‑1 (MQ=255)
                                                                                           cTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATc                                                       >  5:121499/1‑146 (MQ=255)
                                                                                           cTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCgag                                                    <  4:195874/149‑1 (MQ=255)
                                                                                              gTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCgaga                                                   <  6:37840/147‑1 (MQ=255)
                                                                                              gTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCATGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATg                                                 >  3:16226/1‑149 (MQ=255)
                                                                                              gTTCTGATCTGAGAAAAAGGGGTGCAAAGCGACCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGAt                                                  <  8:311657/148‑1 (MQ=255)
                                                                                                 ctgatctgaGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATGTa                                               <  1:243/148‑1 (MQ=255)
                                                                                                       tgaGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCCGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGCCACCAATCGAGATGTAGTCCAcc                                        >  7:350238/1‑149 (MQ=255)
                                                                                                       tgaGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAAGCGAGATGTAGTCCAcc                                        >  6:375577/1‑149 (MQ=255)
                                                                                                                 gggTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATGTAGTCCACCCCCGTCTCgg                              <  5:327715/149‑1 (MQ=255)
                                                                                                                     gCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATGTAGTCCACCCCCGTCTCGGCAAt                          >  7:247567/1‑149 (MQ=255)
                                                                                                                          ttgcaggcAATAAAAAACCGGCGCTTGGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATGTAGTCCACCCCCGTCTCGGCAATCAccc                     >  8:286631/8‑149 (MQ=38)
                                                                                                                          gcgcCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCACTCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATGTAGTCCACCCCCGTCTCGGCAATCAccc                     <  4:270672/149‑1 (MQ=255)
                                                                                                                          gcgcCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGGCATGGCACCAATCGAGATGTAGTCCACCCCCGTCTCGGCAATCAccc                     >  7:183770/1‑149 (MQ=255)
                                                                                                                          gcgcCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCGCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATGTAGTCCACCCCCGTCTCGGCAATCAccc                     <  8:292787/149‑1 (MQ=255)
                                                                                                                             cccccAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCATCAATCGAGATGTAGTCCACCCCCGTCTCGGCAATCACCCGCa                  <  1:258762/149‑1 (MQ=255)
                                                                                                                             cccccAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATGTAGTCCACCCCCGTCTCGGCAATCACCCg                    >  2:146438/1‑147 (MQ=255)
                                                                                                                               cccAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATGTAGTCCACCCCCGTCTCGGCAATCACCCGCAgg                >  1:144116/1‑149 (MQ=255)
                                                                                                                                ccAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCCCCAATCGAGATGTAGTCCCCCCCCGTCTCGGCAATCACCCGCAggg               >  5:361480/1‑149 (MQ=255)
                                                                                                                                 cAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATGTAGTCCACCCCCGTCTCGGCAATCACCCGCAGGGt              >  7:298873/1‑149 (MQ=255)
                                                                                                                                 cAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATGTAGTCCACCCCCGTCTCGGCAATCACCCGCAGGGt              >  1:19921/1‑149 (MQ=255)
                                                                                                                                  aaTAAAAAACCGGCGCTTGGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATGTAGTCCACCCCCGTCTCGGCAATCACCCGCAGGGtc             >  2:196809/1‑149 (MQ=255)
                                                                                                                                  aaTAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATGTAGTCCACCCCCGTCTCGGCAATCACCCGCAGGGtc             >  7:263455/1‑149 (MQ=255)
                                                                                                                                    tAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCCTTCACATCCTTGGTCATGGCACCAATCGAGATGTAGTCCACCCCCGTCTCGGCAATCACCCGCAGGGtcgt           >  5:118821/1‑149 (MQ=255)
                                                                                                                                     aaaaaaCCGGCGCTTGGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATGTAGTCCACCCCCGTCTCGGCAATCACCCGCAGGGtcgtt          >  3:94534/1‑149 (MQ=255)
                                                                                                                                      aaaaaCCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATGTAGTCCACCCCCGTCTCGGCAATCACCCGCAGGGtcgttt         >  2:351873/1‑149 (MQ=255)
                                                                                                                                         aaCCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATGTAGTCCACCCCCGTCTCGGCAATCACCCGCAGGGtcgtttcgt      <  8:247567/149‑1 (MQ=255)
                                                                                                                                          aCCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATGTAGTCCACCCCCGTCTCGGCAATCACCCGCAGGGtcgtttcgtt     <  1:146438/149‑1 (MQ=255)
                                                                                                                                           ccGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATGTAGTCCACCCCCGTCTCGGCAATCACCCGCAGGGTCGTTTCGTTg    <  6:121499/149‑1 (MQ=255)
                                                                                                                                             ggCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATGTAGTCCACCCCCGTCTCGGCAATCACCCGCAGGGTCGTTTCGTTGAc  >  8:106905/1‑149 (MQ=255)
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ACCACGTTTACCGCCAGGCCATCACTTCGGCCGGTGCGGGCTGCATGGCGGCGCTGGATGTCGAGCGCTACCTGGACGGTTTGGCCAACGCCTCGTTCTGATCTGAGAAAAAGGGGTGCAAAGCGCCCCCAATAAAAAACCGGCCAAGCGCCGGTTTTTTATTGCCTGCAATCAGCTCACAGGCTCAGTCGCATCGACAGGTCCACCGCTTTCACATCCTTGGTCATGGCACCAATCGAGATGTAGTCCACCCCCGTCTCGGCAATCACCCGCAGGGTCGTTTCGTTGAC  >  NC_002947/902474‑902763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑