Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 903,026 T→G 7.1% N158T (AAC→ACC)  nadC ← quinolinate phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947903,0260TG 7.1% 203.5 / 4.6 67N158T (AAC→ACC) nadCquinolinate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (32/30);  new base G (3/2);  total (35/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCAGTTCGTCCAGGCTTTCGACCTCGATTTCCACCGGCTTGCCCGGCGCAATGCGGTGTGCTGCGGCCACCGCCTCGGCTACGCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGGTTTTGCGGGTGTCCAGCAGGCGCACCTGGGTGCCGGCTACCAGGTCGGCGAGAAAGCGCGCACGGGTGGCGACCCCTGACAGCA  >  NC_002947/902879‑903167
                                                                                                                                                   |                                                                                                                                             
cgcAGTTCGTCCAGGCTTTCGACCTCGATTTCCACCGGCTTGCCCGGCGCAATGCGGTGTGCTGCGGCCACCGCCTCGGCTACGCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGtt                                                                                                                                              <  4:227083/149‑1 (MQ=255)
    gTTCGTCCAGGCTTTCGACCTCGATTTCCACCGGCTTGCCCGGCGCAATGCGGTGTGCTGCGGCCACCGCCTCGGCTACTCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGCAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCg                                                                                                                                          <  2:335919/149‑1 (MQ=255)
    gTTCGTCCAGGCTTTCGACCTCGATTTCCACCGGCTTGCCCGGCGCAATGCGGTGTGCTGCGGCCACCGCCTCGGCTACGCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCg                                                                                                                                          >  7:269986/1‑149 (MQ=255)
       cGTCCAGGCTTTCGACCTCGATTTCCACCGGCTTGCCCGGCGCAATGCGGTGTGCTGCGGCCACCGCCTCGGCTACGCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGca                                                                                                                                        <  6:235513/148‑1 (MQ=255)
              gCTTTCGACCTCGATTTCCACCGGCTTGCCCGGCGCAATGCGGTGTGCTGCGGCCACCGCCTCGGCTACGCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCg                                                                                                                                 >  4:20383/1‑148 (MQ=255)
                     aCCTCGATTTCCACCGGCTTGCCCGGCGCAATGCGGTGTGCTGCGGCCACCGCCTCGGCTACGCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCAc                                                                                                                         <  1:342530/149‑1 (MQ=255)
                      ccTGGATTTCCAGCGGGTTGCGCGGGGCAATGCGGTGTGCTGCGGCCAGCGCATGGGCTACGAGGCCTCTGGCGGCGATGTGGTTTTCCTTGATCAGGATGGCGTCGTACAGGCCTATGCGGTGTTTGTCGCACCCACCGCAGGTCACt                                                                                                                        <  8:269986/149‑1 (MQ=255)
                      ccTCGATTTCCACCGGCTTGCCCGGCGCAATGCGGTGTGCTGCGGCCACCGCCTCGGCTACGCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACt                                                                                                                        >  1:75646/1‑149 (MQ=255)
                        tCGATTTCCACCGGCTTGCCCGGCGCAATGCGGTGTGCTGCGGCCACCGCCTCGGCTACGCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGGTGCGACAACCACCGCatag                                                                                                                            >  6:349057/1‑140 (MQ=255)
                        tCGATTTCCACCGGCTTGCCCGGCGCAATGCGGTGTGCTGCGGCCACCGCCTCGGCTACGCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGGTGCGACAACCACCGCatag                                                                                                                            <  5:349057/143‑4 (MQ=255)
                          gATTTCCACCGGCTTGCCCGGCGCAATGCGGTGTGCTGCGGCCACCGCCTCGGCTACGCCGCCGCTGGCGGTGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCg                                                                                                                     >  1:214045/1‑148 (MQ=255)
                            tttCCACCGGCTTGCCCGGCGCAATGCGGTGTGCTGCGGCCACCGCCTCGGCTACGCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTAt                                                                                                                  <  6:188920/149‑1 (MQ=255)
                               ccACCGGCTTGCCCGGCGCAATGCGGTGTGCTGCGGCCACCGCCTCGGCTACGCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTAtttt                                                                                                               >  2:199577/1‑149 (MQ=255)
                                  ccGGCTTGCCCGGCGCAATGCGGTGTGCTGCGGCCACCGCCTCGGCTACGCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTgc                                                                                                            <  7:324849/149‑1 (MQ=255)
                                     gCTTGCCCGGCGCAATGCGGTGTGCTGCGGCCACCGCCTCGGCTACGCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCCCTGCGTATTTTTgcgc                                                                                                          >  5:317244/1‑148 (MQ=255)
                                           ccGGCGCAATGCGGTGTGCTGCGGCCACCGCCTCGGCTACGCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTc                                                                                                                                           >  3:354116/1‑109 (MQ=255)
                                           ccGGCGCAATGCGGTGTGCTGCGGCCACCGCCTCGGCTACGCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTc                                                                                                                                           <  4:354116/109‑1 (MQ=255)
                                           ccGGCGCAATGCGGTGTGCTGCGGCCACCGCCTCGGCTACGCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGgcgc                                                                                                   <  5:2748/149‑1 (MQ=255)
                                           ccGGCGCAATGCGGTGTGCTGCGGCCACCGCCTCGGCTACGCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGgcg                                                                                                    >  5:68759/1‑148 (MQ=255)
                                           ccGGCGCAATGCGGTGTGCTGCGGCCACCGCCTCGGCTACGCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGgcg                                                                                                    >  6:99354/1‑148 (MQ=255)
                                            cGGCGCAATGCGGTGTGCTGCGGCCACCGCCTCGGCTACGCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGgcg                                                                                                    >  6:24981/1‑147 (MQ=255)
                                            cGGCGCAATGCGGTGTGCTGCGGCCACCGCCTCGGCTACGCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGgcg                                                                                                    >  6:30472/1‑147 (MQ=255)
                                                  aaTGCGGTGTGCTGCGGCCACCGCCTCGGCTACGCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTgc                                                                                                            <  6:258916/133‑1 (MQ=255)
                                                  aaTGCGGTGTGCTGCGGCCACCGCCTCGGCTACGCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTgc                                                                                                            >  5:258916/1‑133 (MQ=255)
                                                  aaTGCGGTGTGCTGCGGCCACCGCCTCGGCTACGCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCg                                                                                            <  5:24981/149‑1 (MQ=255)
                                                  aaTGCGGTGTGCTGCGGCCACCGCCTCGGCTACGCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCg                                                                                            <  5:30472/149‑1 (MQ=255)
                                                      cGGTGTGCTGCGGCCACCGCCTCGGCTACGCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCa                                                                                         <  6:66366/148‑1 (MQ=255)
                                                       ggTGTGCTGCGGCCACCGCCTCGGCTACGCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTgcg                                                                                                           >  5:101751/1‑129 (MQ=255)
                                                       ggTGTGCTGCGGCCACCGCCTCGGCTACGCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTgcg                                                                                                           <  6:101751/129‑1 (MQ=255)
                                                        gtgtGCTGCGGCCACCGCCTCGGCTACGCCGCCGCTGGCGGTGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAggg                                                                                      <  2:214045/149‑1 (MQ=255)
                                                               gcGGCCACCGCCTCGGCTACGCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGGTTTTGCg                                                                               >  7:214647/1‑149 (MQ=255)
                                                                cGGCCACCGCCTCGGCTACGCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGGTTTTGCgg                                                                              <  1:348812/149‑1 (MQ=255)
                                                                   ccACCGCCTCGGCTACGCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGGTTTTGCGGgtg                                                                           >  4:43022/1‑149 (MQ=255)
                                                                     aCCGCCTCGGCTACGCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGGTTTTGCGGGTGTc                                                                         >  5:270076/1‑149 (MQ=255)
                                                                           tCGGCTACGCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGGTTTTGCGGGTGTCcagcag                                                                   >  1:27689/1‑149 (MQ=255)
                                                                             ggCTACGCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGGTTTTGCGGGTGTCCAGCAGgc                                                                 <  2:27689/149‑1 (MQ=255)
                                                                                atcgccgccagCGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGGTTTTGCGGGTGTCCAGCAGGCGCa                                                              >  4:334530/3‑149 (MQ=255)
                                                                                  cgccgccgcTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGGTTTTGCGGGTGTCCAGCAGGCGCAc                                                             <  3:188560/148‑1 (MQ=255)
                                                                                     cgccgcTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGGTTTTGCGGGTGTCCAGCAGGCGCACCTgg                                                         <  2:75646/149‑1 (MQ=255)
                                                                                     cgccgcTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGGTTTTGCGGGTGTCCAGCAGGCGCACCTgg                                                         <  1:199577/149‑1 (MQ=255)
                                                                                             gcggcgATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGGACCCACCGCAGGTCACTGCTTATTTTTGCGCCAGGCGGAGGCCCGGCAGGGTTTTGCGGGTGTCCAGCAGGCGCACCTGGGTGCCg                                                   <  1:190539/147‑1 (MQ=255)
                                                                                             gcggcgATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGGTTTTGCGGGTGTCCAGCAGGCGCACCTGGGTGCCgg                                                  <  3:43022/148‑1 (MQ=255)
                                                                                                   aTGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGGTTTTGCGGGTGTCCAGCAGGCGCACCTGGGTGCCGGCTACCAg                                           >  2:61957/1‑149 (MQ=255)
                                                                                                    tgtgGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGGTTTTGCGGGTGTCCAGCAGGCGCACCTGGGTGCCGGCTACCAgg                                          >  5:82241/1‑149 (MQ=255)
                                                                                                     gtgGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGGTTTTGCGGGTGTCCAGCAGGCGCACCTGGGTGCCGGCTACCAGGt                                         >  1:80001/1‑149 (MQ=255)
                                                                                                       ggTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGGTTTTGCGGGTGTCCAGCAGGCGCACCTGGGTGCCGGCTACCAGGTCg                                       >  5:276994/1‑149 (MQ=255)
                                                                                                              cTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGGTTTTGCGGGTGTCCAGCAGGCGCACCTGGGTGCCGGCTACCAGGTCGGCgaga                                 >  6:69970/1‑148 (MQ=255)
                                                                                                               ttGATCAGGAAGGCGTCGTACATGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGGTTTTGCGGGTGTCCAGCAGGCGCACCTGGGTGCCGGCTACCAGGTCGGCgaga                                 >  7:234507/1‑147 (MQ=255)
                                                                                                               ttGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAgg                                                                                       <  8:199353/93‑1 (MQ=255)
                                                                                                               ttGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAgg                                                                                       >  7:199353/1‑93 (MQ=255)
                                                                                                                 gATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGGTTTTGCGGGTGTCCAGCAGGCGCACCTGGGTGCCGGCTACCAGGTCGGCGAGaaa                               <  5:99354/147‑1 (MQ=255)
                                                                                                                 gATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGGTTTTGCGGGTGTCCAGCAGGCGCACCTGGGTGCCGGCTACCAGGTCGGCGAGaaa                               <  8:234507/147‑1 (MQ=255)
                                                                                                                      ggAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGGTTTTGCGGGTGTCCAGCAGGCGCACCTGGGTGCCGGCTACCAGGTCGGCGAGAAAGCGCGCa                        >  2:199452/1‑149 (MQ=255)
                                                                                                                       gAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCTCCAGGCGCAGGCCCGGCAGGGTTTTGCGGGTGTCCAGCAGGCGCACCTGGGTGCCGGCTACCAGGTCGGCGAGAAAGCGCGAAc                       >  8:169818/1‑149 (MQ=255)
                                                                                                                             gtcgtACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGGTTTTGCGGGTGTCCAGCAGGCGCACCTGGGTGCCGGCTACCAGGTCGGCGAGAAAGCGCGCACGGGTgg                 <  1:199452/149‑1 (MQ=255)
                                                                                                                             gtcgtACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGGTTTTGCGGGTGTCCAGCAGGCGCACCTGGGTGCCGGCTACCAGGTCGGCGAGAAAGCGCGCACGGGTgg                 <  6:68759/149‑1 (MQ=255)
                                                                                                                             gtcgtACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGGTTTTGCGGGTGTCCAGCAGGCGCACCTGGGTGCCGGCTACCAGGTCGGCGAGAAAGCGCGCACGGGTgg                 <  6:317244/149‑1 (MQ=255)
                                                                                                                              tcgtACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGGTTTTGCGGGTGTCCAGCAGGCGCACCTGGGTGCCGGCTACCAGGTCGGCGAGAAAGCGCGCACGGGTGGc                >  8:96907/1‑149 (MQ=255)
                                                                                                                                gtACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGGTTTTGCGGGTGTCCAGCAGGCGCACCTGGGTGCCGGCTACCAGGTCGGCGAGAAAGCGCGCACGGGTGGCGa              <  8:214647/149‑1 (MQ=255)
                                                                                                                                  aCAGGCCTATGCGGTGGTTGTCGCACCAACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCATGCCCGGCAGGGTTTTGCGGGTGTCCAGCAGGCGCACCTGGGTGCCGGATACCAGGTCGGCGAGAAAGCGCGAAAGGGTGGCGAcc            >  3:29717/1‑149 (MQ=255)
                                                                                                                                   cAGGCCTATGCGGTGGGTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGTGTTTTGCGGGTGTCCAGCAGGCGCACCTGGGTGCCGGCTACCAGGTCGGCGAGAAAGCGCGCACGGGTGGCTAcc            <  2:48202/148‑1 (MQ=255)
                                                                                                                                        cTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGAAGGGTTTTGCGGGTGTCCAGCAGGCGCACCTGGGTGCCGGCTACAAGGTCGGCGAGAAAGCGCGCACGGGTGGCGACCCCTGa       >  3:147702/1‑148 (MQ=255)
                                                                                                                                         tATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGGTTTTGCGGGTGTCCAGCAGGCGCACCTGGGTGCCg                                                   <  6:101989/103‑1 (MQ=255)
                                                                                                                                         tATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGGTTTTGCGGGTGTCCAGCAGGCGCACCTGGGTGCCg                                                   >  5:101989/1‑103 (MQ=255)
                                                                                                                                         tATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGGTTTTGCGGGTGTCCAGCAGGCGCACCTGGGTGCCGGCTACCAGGTCGGCGAGAAAGCGCGCACGGGTGGCGACCCCTGa       >  3:174015/1‑147 (MQ=255)
                                                                                                                                          ctGCGGTGGGTGCGACAACCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGGTTTTGCGGGTGTCCAGCAGGCGCACCTGGGTGCCGGCTACCAGGTCGGCGAGAAAGCGCGCACGGGTGGCGACCCCTGAcag    >  3:311715/2‑149 (MQ=255)
                                                                                                                                           tGCGGTGGGTGCGACAACCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGATTTTGCGGGTGTCCAGCAGGCGCACCTGGGTGCCGGCTACCAGGTCGGCGAGAAAGCGCGCACGGGTGGCGACCCCTGAcag    >  1:299351/1‑148 (MQ=255)
                                                                                                                                             cGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGGTTTTGCGGGTGTCCAGCAGGCGCACCTGGGTGCCGGCTACCAGGTCGGCGAGAAAGCGCGCACGGGTGGCGACCCCTGAcagca  <  6:276994/148‑1 (MQ=255)
                                                                                                                                              ggtggtTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGGTTTTGCGGGTGTCCAGCAGGCGCACCTGGGTGCCGGCTACCAGGTCGGCGAGAAAGCGCGCACGGGTGGCGACCCCt         <  7:294581/140‑1 (MQ=255)
                                                                                                                                              ggtggtTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGGTTTTGCGGGTGTCCAGCAGGCGCACCTGGGTGCCGGCTACCAGGTCGGCGAGAAAGCGCGCACGGGTGGCGACCCCt         >  8:294581/1‑140 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
CGCAGTTCGTCCAGGCTTTCGACCTCGATTTCCACCGGCTTGCCCGGCGCAATGCGGTGTGCTGCGGCCACCGCCTCGGCTACGCCGCCGCTGGCGGCGATGTGGTTTTCCTTGATCAGGAAGGCGTCGTACAGGCCTATGCGGTGGTTGTCGCACCCACCGCAGGTCACTGCGTATTTTTGCGCCAGGCGCAGGCCCGGCAGGGTTTTGCGGGTGTCCAGCAGGCGCACCTGGGTGCCGGCTACCAGGTCGGCGAGAAAGCGCGCACGGGTGGCGACCCCTGACAGCA  >  NC_002947/902879‑903167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑