Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 925,753 T→C 9.3% A291A (GCA→GCG)  PP_0805 ← outer membrane efflux protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947925,7530TC 9.3% 146.6 / 3.4 53A291A (GCA→GCG) PP_0805outer membrane efflux protein
Reads supporting (aligned to +/- strand):  ref base T (22/26);  new base C (2/3);  total (24/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACGTTCAGGTAGATGCCCGCCTGGGTGCGGTCCAGCCGTGCGTTATCGCGGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGG  >  NC_002947/925606‑925891
                                                                                                                                                   |                                                                                                                                          
gACGTTCAGGTAGATGCCCGCCTGGGTGCGGTCCAGCCGTGCGTTATCGCGGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCtgtg                                                                                                                                           >  6:224124/1‑149 (MQ=255)
gACGTTCAGGTAGATGCCCGCCTGGGTGCGGTCCAGCCGTGCGTTATCGCGGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCtgt                                                                                                                                            <  3:92553/148‑1 (MQ=255)
     gcAGGTAGATGCCCGCCTGGGTGCGGTCCAGCCGTGCGTTATCGCGGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCGCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTgcgcgc                                                                                                                                      <  3:82055/148‑1 (MQ=255)
       aGGTAGATGCCCGCCTGGGTGCGGTCCAGCCGTGCGTTAGCGCGGGTGGAGTCGGCGGCCAGGTAATGCTTGCCCGGCGGGTGCAACGCCCGGGTAGGGGAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTg                                                                                                                                    <  3:217996/149‑1 (MQ=255)
          ttgATGCCCGCCTGGGTGCGGTCCAGCCGTGCGTTATCGCGGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTggg                                                                                                                                  <  8:121562/146‑1 (MQ=255)
          tAGATGCCCGCCTGGGTGCGGTCCAGCCGTGCGTTATCGCGGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTgggc                                                                                                                                 <  5:224124/149‑1 (MQ=255)
            gATGCCCGCCTGGGTGCGGTCCAGCCGTGCGTTATCGCGGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCAGCGCGCACAgggcca                                                                                                                               <  1:208376/149‑1 (MQ=255)
              tGCCCGCCTGGGTGCGGTCCAGCCGTGCGTTATCGCGGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTgggccagg                                                                                                                             <  3:97770/149‑1 (MQ=255)
               gCCCGCCTGGGTGCGGTCCAGCCGTGCGTTATCGCGGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTgggccaggg                                                                                                                            >  5:54880/1‑149 (MQ=255)
                   gCCTGGGTGCGGTCCAGCCGTGCGTTATCGCGGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCAt                                                                                                                        >  3:105985/1‑149 (MQ=255)
                        ggTGCGGTCCAGCCGTGCGTTATCGCGGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCa                                                                                                                   <  3:163356/149‑1 (MQ=255)
                            cGGTCCAGCCGTGCGTTATCGCGGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCAcggc                                                                                                               >  8:286710/1‑149 (MQ=255)
                            cGGTCCAGCCGTGCGTTATCGCGGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCAcggc                                                                                                               <  7:71600/149‑1 (MQ=255)
                            cGGTCCAGCCGTGCGTTATCGCGGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCAcggc                                                                                                               <  6:231594/149‑1 (MQ=255)
                                   gCCGTGCGTTATCGCGGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAgg                                                                                                        <  1:349698/149‑1 (MQ=255)
                                       tGCGTTATCGCGGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGCCCTGGCCCAGCGCGCACAgggccaggccg                                                                                                                          <  1:314078/127‑4 (MQ=39)
                                       tGCGTTATCGCGGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGCCCTGGCCCAGCGCGCACAgggccaggccg                                                                                                                          >  2:314078/1‑124 (MQ=39)
                                                 cgGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAg                                                                                                         <  7:43647/134‑1 (MQ=255)
                                                 cgGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAg                                                                                                         >  8:43647/1‑134 (MQ=255)
                                                  ggTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCtgt                                                                                         >  5:217150/1‑149 (MQ=255)
                                                  ggTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCtgt                                                                                         >  4:331903/1‑149 (MQ=255)
                                                      gTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTTGGCCAGGGCCCTCAGCACGGCGGGCAGGCGATCATCACCCtctgaat                                                                                     >  7:175681/1‑146 (MQ=255)
                                                               gtcCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTg                                                                             >  1:109017/1‑148 (MQ=255)
                                                                  cAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCa                                                                           >  3:98936/1‑147 (MQ=255)
                                                                  cAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCa                                                                           <  4:98936/147‑1 (MQ=255)
                                                                     gTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCt                                                                      >  3:27340/1‑149 (MQ=255)
                                                                         tGATTGACATGGGTTTGCAAAGACAGTGTAGGGTAGGCCTCCTCGTTGTCCTGGGCCAGCTCGGGCTGGCCCTGTGCGCGCTGTGCCAGGGCAATCAGCACGGCGGGCAGGCGATCATCAACCTGTGCA‑GGCGCTTTCAGGCCTTGTc                                                                  <  8:175681/148‑1 (MQ=255)
                                                                          gATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGTGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTc                                                                  >  1:345683/1‑148 (MQ=255)
                                                                                      gTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGt                                                     <  7:286710/149‑1 (MQ=255)
                                                                                      gTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGt                                                     <  6:16424/149‑1 (MQ=255)
                                                                                       ttGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTgggccagg                                                                                                                             <  8:17745/76‑1 (MQ=255)
                                                                                       ttGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTg                                                    <  2:109017/149‑1 (MQ=255)
                                                                                       ttGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCATGGCCCTGTGCGCGCTgggccagg                                                                                                                             >  7:17745/1‑76 (MQ=255)
                                                                                         gCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCtt                                                                              <  6:257422/121‑1 (MQ=255)
                                                                                         gCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCtt                                                                              >  5:257422/1‑121 (MQ=255)
                                                                                           aaCGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGcc                                                >  8:337680/1‑149 (MQ=255)
                                                                                           aaCGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGcc                                                >  3:56408/1‑149 (MQ=255)
                                                                                           aaCGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGcc                                                >  8:34036/1‑149 (MQ=255)
                                                                                                   ggtagggtagGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGc                                          >  7:168157/1‑147 (MQ=255)
                                                                                                   ggtagggtagGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGc                                          >  1:84019/1‑147 (MQ=255)
                                                                                                     tagggtaggCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGt                                      <  7:337680/149‑1 (MQ=255)
                                                                                                      agggtaggCCTCCGCCTTGGCCTGGGCCAGATCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCCCGGCTGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTg                                     >  2:167728/1‑149 (MQ=255)
                                                                                                           aggCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAg                                                  <  3:341000/131‑1 (MQ=255)
                                                                                                           aggCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAg                                                  >  4:341000/1‑131 (MQ=255)
                                                                                                            ggCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAg                               <  4:56408/149‑1 (MQ=255)
                                                                                                             gCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCGGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAgg                              <  6:54880/149‑1 (MQ=255)
                                                                                                             gCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAgg                              <  3:331903/149‑1 (MQ=255)
                                                                                                                tCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAgggg                            >  3:38954/1‑148 (MQ=255)
                                                                                                                         gCCTGGGCCAGCGCGGCCAGGCCCGGCGCGCGCGCGGCCAGGGCCATCAGCACGGCGGGCAGGCGAGCATCACCCGGTGCCGGGCGCTTGCCGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGAcc                  <  8:203627/149‑1 (MQ=255)
                                                                                                                              ggCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCa             <  2:133101/149‑1 (MQ=255)
                                                                                                                                       ggCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGcgccgc      <  4:38954/147‑1 (MQ=255)
                                                                                                                                         ccTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAgg  <  4:105985/149‑1 (MQ=255)
                                                                                                                                         ccTGGCCCAGCGCGCACAGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAgg  >  2:283986/1‑149 (MQ=255)
                                                                                                                                          cTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGCGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATc              <  7:284232/136‑1 (MQ=255)
                                                                                                                                          cTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGCGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATc              >  8:284232/1‑136 (MQ=255)
                                                                                                                                                   |                                                                                                                                          
GACGTTCAGGTAGATGCCCGCCTGGGTGCGGTCCAGCCGTGCGTTATCGCGGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGG  >  NC_002947/925606‑925891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑