Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 925,760 T→A 7.6% Q289L (CAG→CTG)  PP_0805 ← outer membrane efflux protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947925,7600TA 7.6% 143.3 / 2.7 53Q289L (CAG→CTG) PP_0805outer membrane efflux protein
Reads supporting (aligned to +/- strand):  ref base T (22/26);  new base A (2/2);  total (24/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.12e-01

AGGTAGATGCCCGCCTGGGTGCGGTCCAGCCGTGCGTTATCGCGGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAG  >  NC_002947/925613‑925903
                                                                                                                                                   |                                                                                                                                               
aGGTAGATGCCCGCCTGGGTGCGGTCCAGCCGTGCGTTAGCGCGGGTGGAGTCGGCGGCCAGGTAATGCTTGCCCGGCGGGTGCAACGCCCGGGTAGGGGAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTg                                                                                                                                                <  3:217996/149‑1 (MQ=255)
   ttgATGCCCGCCTGGGTGCGGTCCAGCCGTGCGTTATCGCGGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTggg                                                                                                                                              <  8:121562/146‑1 (MQ=255)
   tAGATGCCCGCCTGGGTGCGGTCCAGCCGTGCGTTATCGCGGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTgggc                                                                                                                                             <  5:224124/149‑1 (MQ=255)
     gATGCCCGCCTGGGTGCGGTCCAGCCGTGCGTTATCGCGGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCAGCGCGCACAgggcca                                                                                                                                           <  1:208376/149‑1 (MQ=255)
       tGCCCGCCTGGGTGCGGTCCAGCCGTGCGTTATCGCGGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTgggccagg                                                                                                                                         <  3:97770/149‑1 (MQ=255)
        gCCCGCCTGGGTGCGGTCCAGCCGTGCGTTATCGCGGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTgggccaggg                                                                                                                                        >  5:54880/1‑149 (MQ=255)
            gCCTGGGTGCGGTCCAGCCGTGCGTTATCGCGGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCAt                                                                                                                                    >  3:105985/1‑149 (MQ=255)
                 ggTGCGGTCCAGCCGTGCGTTATCGCGGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCa                                                                                                                               <  3:163356/149‑1 (MQ=255)
                     cGGTCCAGCCGTGCGTTATCGCGGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCAcggc                                                                                                                           >  8:286710/1‑149 (MQ=255)
                     cGGTCCAGCCGTGCGTTATCGCGGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCAcggc                                                                                                                           <  7:71600/149‑1 (MQ=255)
                     cGGTCCAGCCGTGCGTTATCGCGGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCAcggc                                                                                                                           <  6:231594/149‑1 (MQ=255)
                            gCCGTGCGTTATCGCGGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAgg                                                                                                                    <  1:349698/149‑1 (MQ=255)
                                tGCGTTATCGCGGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGCCCTGGCCCAGCGCGCACAgggccaggccg                                                                                                                                      <  1:314078/127‑4 (MQ=39)
                                tGCGTTATCGCGGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGCCCTGGCCCAGCGCGCACAgggccaggccg                                                                                                                                      >  2:314078/1‑124 (MQ=39)
                                          cgGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAg                                                                                                                     >  8:43647/1‑134 (MQ=255)
                                          cgGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAg                                                                                                                     <  7:43647/134‑1 (MQ=255)
                                           ggTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCtgt                                                                                                     >  4:331903/1‑149 (MQ=255)
                                           ggTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCtgt                                                                                                     >  5:217150/1‑149 (MQ=255)
                                               gTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTTGGCCAGGGCCCTCAGCACGGCGGGCAGGCGATCATCACCCtctgaat                                                                                                 >  7:175681/1‑146 (MQ=255)
                                                        gtcCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTg                                                                                         >  1:109017/1‑148 (MQ=255)
                                                           cAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCa                                                                                       >  3:98936/1‑147 (MQ=255)
                                                           cAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCa                                                                                       <  4:98936/147‑1 (MQ=255)
                                                              gTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCt                                                                                  >  3:27340/1‑149 (MQ=255)
                                                                  tGATTGACATGGGTTTGCAAAGACAGTGTAGGGTAGGCCTCCTCGTTGTCCTGGGCCAGCTCGGGCTGGCCCTGTGCGCGCTGTGCCAGGGCAATCAGCACGGCGGGCAGGCGATCATCAACCTGTGCA‑GGCGCTTTCAGGCCTTGTc                                                                              <  8:175681/148‑1 (MQ=255)
                                                                   gATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGTGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTc                                                                              >  1:345683/1‑148 (MQ=255)
                                                                               gTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGt                                                                 <  7:286710/149‑1 (MQ=255)
                                                                               gTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGt                                                                 <  6:16424/149‑1 (MQ=255)
                                                                                ttGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTgggccagg                                                                                                                                         <  8:17745/76‑1 (MQ=255)
                                                                                ttGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTg                                                                <  2:109017/149‑1 (MQ=255)
                                                                                ttGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCATGGCCCTGTGCGCGCTgggccagg                                                                                                                                         >  7:17745/1‑76 (MQ=255)
                                                                                  gCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCtt                                                                                          <  6:257422/121‑1 (MQ=255)
                                                                                  gCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCtt                                                                                          >  5:257422/1‑121 (MQ=255)
                                                                                    aaCGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGcc                                                            >  8:34036/1‑149 (MQ=255)
                                                                                    aaCGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGcc                                                            >  8:337680/1‑149 (MQ=255)
                                                                                    aaCGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGcc                                                            >  3:56408/1‑149 (MQ=255)
                                                                                            ggtagggtagGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGc                                                      >  7:168157/1‑147 (MQ=255)
                                                                                            ggtagggtagGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGc                                                      >  1:84019/1‑147 (MQ=255)
                                                                                              tagggtaggCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGt                                                  <  7:337680/149‑1 (MQ=255)
                                                                                               agggtaggCCTCCGCCTTGGCCTGGGCCAGATCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCCCGGCTGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTg                                                 >  2:167728/1‑149 (MQ=255)
                                                                                                    aggCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAg                                                              <  3:341000/131‑1 (MQ=255)
                                                                                                    aggCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAg                                                              >  4:341000/1‑131 (MQ=255)
                                                                                                     ggCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAg                                           <  4:56408/149‑1 (MQ=255)
                                                                                                      gCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCGGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAgg                                          <  6:54880/149‑1 (MQ=255)
                                                                                                      gCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAgg                                          <  3:331903/149‑1 (MQ=255)
                                                                                                         tCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAgggg                                        >  3:38954/1‑148 (MQ=255)
                                                                                                                  gCCTGGGCCAGCGCGGCCAGGCCCGGCGCGCGCGCGGCCAGGGCCATCAGCACGGCGGGCAGGCGAGCATCACCCGGTGCCGGGCGCTTGCCGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGAcc                              <  8:203627/149‑1 (MQ=255)
                                                                                                                       ggCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCa                         <  2:133101/149‑1 (MQ=255)
                                                                                                                                ggCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGcgccgc                  <  4:38954/147‑1 (MQ=255)
                                                                                                                                  ccTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAgg              <  4:105985/149‑1 (MQ=255)
                                                                                                                                  ccTGGCCCAGCGCGCACAGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAgg              >  2:283986/1‑149 (MQ=255)
                                                                                                                                   cTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGCGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATc                          >  8:284232/1‑136 (MQ=255)
                                                                                                                                   cTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGCGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATc                          <  7:284232/136‑1 (MQ=255)
                                                                                                                                              cgcgcTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAg  <  4:27340/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
AGGTAGATGCCCGCCTGGGTGCGGTCCAGCCGTGCGTTATCGCGGTTGTAGTCGTCGTCCAGGTAATGATTGACCTGCGGTTGCAACGACAGGGTAGGGTAGGCCTCCGCCTTGGCCTGGGCCAGCTCGGCCTGGCCCTGTGCGCGCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAG  >  NC_002947/925613‑925903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑