Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	928,853	G→C	6.0%	P5590R (CCG→CGG)	PP_0806 ←	surface adhesion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	928,853	0	G	C	6.0%	210.8 / 3.5	67	P5590R (CCG→CGG)	PP_0806	surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base G (34/29);  new base C (2/2);  total (36/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.78e-01

CAGGTTGACTGTGGCCGTGGCCAGGTTGTCGCTGGCGATGACCGGCGCGTTGGCATCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGAGGGTACCGCCGTCGGCACTGAACAGCAGGTTGCCGG  >  NC_002947/928705‑929000                                                                                                                                                      |                                                                                                                                                    cAGGTTGACTGTGGCCGTGGCCAGGTTGTCGCTGGCGATGACCGGCGCGTTGGCATCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGACGCGCGGG‑TCGCTGAGGGTCACCGCAAGGCTCTGGCCGTTGTACTGAGGCg                                                                                                                                                     >  3:256613/1‑149 (MQ=255)         cTGTGGCCGTGGCCAGGTTGTCGCTGGCGATGACCGGCGCGTTGGCATCCACATCCGGTGTAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACGGAAAGGATCTGGCCGTCGTCCTGAGGCGGGCTCAgg                                                                                                                                             <  3:304659/149‑1 (MQ=255)            tggccgtggccAGGTTGTCGCTGGCGATGACCGGCGCGTTGGCATCCACATCCGGTGCAGTGACGGTGACGTTGCCCGACACGTTGCCGCGCGGGCTCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGCCCGCCTCAgga                                                                                                                                            <  1:92413/148‑2 (MQ=255)            tggcagtggccAGGTTGTAGCTTGCGATGACCGGCGCGTTGGCATCCACATCCGGTGAAGTGCCGTTGACGTTGCCCGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGt                                                                                                                                           >  4:365816/1‑148 (MQ=255)              gccgtggccAGGTTGTCGCTGGCGATGACCGGCGCGTTGGCATCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCAc                                                                                                                                        <  4:173359/149‑1 (MQ=255)                 gtggccAGGTTGTCGCTGGCGATGACCGGCGCGTTGGCATCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGt                                                                                                                                     <  8:180555/149‑1 (MQ=255)                  tggccAGGTTGTCGCTGGCGATGACCGGCGCGTTGGCATCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGt                                                                                                                                     >  8:495/1‑148 (MQ=255)                      cAGGTTGTCGCTGGCGATGACCGGCGCGTTGGCATCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAgg                                                                                                                                >  1:154484/1‑149 (MQ=255)                      cAGGTTGTCGCTGGCGATGACCGGCGCGTTGGCATCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAgg                                                                                                                                >  1:244382/1‑149 (MQ=255)                         gTTGTCGCTGGCGATGACCGGCGCGTTGGCATCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTc                                                                                                                                                <  8:260461/130‑1 (MQ=255)                         gTTGTCGCTGGCGATGACCGGCGCGTTGGCATCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTc                                                                                                                                                >  7:260461/1‑130 (MQ=255)                         gTTGTCGCTGGCGATGACCGGCGCGTTGGCATCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGc                                                                                                                             >  3:322317/1‑149 (MQ=255)                          ttGTCGCTGGCGATGACCGGCGCGTTGGCATCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGcc                                                                                                                            <  2:68860/149‑1 (MQ=255)                               gCTGGCGATGACCGGCGCGTTGGCATCCACATCCGGTGGAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGG‑GCGCTGAGGGTCACCGAAAGGATCTGGGCTTTGTGCTGAGGCGGGCTCAGGGTCTCGGTGAAGGTGCCGTcc                                                                                                                        <  3:61397/148‑1 (MQ=255)                               gCTGGCGATGACCGGCGCGTTGGCATCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTcc                                                                                                                        <  5:155600/148‑1 (MQ=255)                                         aCCGGCGCGTTGGCATCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGt                                                                                                             >  2:288490/1‑149 (MQ=255)                                                cgTTGGCATCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTc                                                                                                      <  2:154484/149‑1 (MQ=255)                                                  ttGGCATCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATATGTCCGTTGTCCTGAGGCGGGCTCAGGGTCCAGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGGCTg                                                                                                    >  8:291000/1‑149 (MQ=255)                                                    ggCATCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTgc                                                                                                   <  8:34141/148‑1 (MQ=255)                                                       aTCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCACCGTACgcagcg                                                                                               >  7:143881/1‑148 (MQ=255)                                                       aTCCACATCCGGTGCAGTGAAGTTGACGTTGCCCGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTgcagca                                                                                               >  6:150514/1‑149 (MQ=255)                                                        tCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCAc                                                                                              <  5:80346/149‑1 (MQ=255)                                                           acaTCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACgg                                                                                            >  4:266199/1‑148 (MQ=255)                                                           acaTCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACgg                                                                                            >  6:255098/1‑148 (MQ=255)                                                                        tgacgttgacgtTGCCCGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGgcg                                                                                 <  2:325209/146‑1 (MQ=255)                                                                        tgacgttgacgtTGCCCGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGgcg                                                                                 >  1:325209/1‑146 (MQ=255)                                                                            gttgacgttgCCCGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGACGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACAGCAGGCGCTGCGTa                                                                          >  4:286284/1‑149 (MQ=255)                                                                             ttgacgttgCCCGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTgc                                                                                                   <  3:2437/123‑1 (MQ=255)                                                                             ttgacgttgCCCGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTgc                                                                                                   >  4:2437/1‑123 (MQ=255)                                                                             ttgacgttgCCCGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTac                                                                         >  1:291254/1‑149 (MQ=255)                                                                                acgttgCCCGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTacggt                                                                      <  4:340135/149‑1 (MQ=255)                                                                                       ccGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTacggtgacggtg                                                               <  8:319272/149‑1 (MQ=255)                                                                                       ccGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTacggtgacggtg                                                               <  1:288490/149‑1 (MQ=255)                                                                                       ccGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTacggtgacggtg                                                               >  7:319272/1‑149 (MQ=255)                                                                                        cGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTacggtgacggtg                                                               >  8:84506/1‑148 (MQ=255)                                                                                        cGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTgg                                                              >  8:79294/1‑149 (MQ=255)                                                                                               ttGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAgg                                                                                                                                             <  8:8479/63‑1 (MQ=255)                                                                                               ttGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAgg                                                                                                                                             >  7:8479/1‑63 (MQ=255)                                                                                               ttGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTgc                                                                                                   >  1:231974/1‑105 (MQ=255)                                                                                               ttGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTgc                                                                                                   <  2:231974/105‑1 (MQ=255)                                                                                                    gcgcgGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGc                                                  >  1:169277/1‑149 (MQ=255)                                                                                                    gcgcgGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGc                                                  >  5:23833/1‑149 (MQ=255)                                                                                                     cgcgGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGTCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGcc                                                 <  5:152421/149‑1 (MQ=255)                                                                                                         gg‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTa                                             <  4:322317/149‑1 (MQ=255)                                                                                                               cTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTacggtgacggtg                                                               <  7:65115/126‑1 (MQ=255)                                                                                                               cTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGt                                        <  2:291254/149‑1 (MQ=255)                                                                                                               cTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGt                                        >  4:78983/1‑149 (MQ=255)                                                                                                               cTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTCCAGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTacggtgacggtg                                                               >  8:65115/1‑126 (MQ=255)                                                                                                                 gAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGa                                      <  3:266199/149‑1 (MQ=255)                                                                                                                  aGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGACGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGa                                      <  7:291000/148‑1 (MQ=255)                                                                                                                    ggTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGAggg                                   >  2:67033/1‑149 (MQ=255)                                                                                                                      tCACCGAAACGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGCTGCCGTACCCCTGCACCGACGCGGTCTGCAGCACGGTAACGACGCCGCTTCGTACGCTGACGGACTCAACCACTTCGACAGTAACGGTGAgcct                                  >  4:333971/1‑145 (MQ=255)                                                                                                                        aCCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGAGGGTAcc                               <  7:79294/149‑1 (MQ=255)                                                                                                                        aCCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGAGGGTAcc                               <  7:84506/149‑1 (MQ=255)                                                                                                                         ccGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGAGGGTAccg                              >  6:46290/1‑149 (MQ=255)                                                                                                                         ccGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGACTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGAGGGTAccg                              >  3:144833/1‑149 (MQ=255)                                                                                                                          cGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGAGGGTAccgc                             >  8:330429/1‑149 (MQ=255)                                                                                                                                      gCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGCCGGTGGCACCCACTTCGCCAGTACCGGTGAGGGTACCGCCGTCGGCACTg                  >  8:317495/1‑148 (MQ=255)                                                                                                                                      gCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGAGGGTACCGCCGTCGGCACTg                  >  4:262968/1‑148 (MQ=255)                                                                                                                                       ccGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGAGGGTACCGCCGTCGGCACTGaa                <  3:262968/149‑1 (MQ=255)                                                                                                                                         gTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGAGGGTACCGCCGTCGGCACTGAAca              <  2:169277/149‑1 (MQ=255)                                                                                                                                          tgacccTGAGCCCGCCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGAg                                     <  8:114552/121‑1 (MQ=255)                                                                                                                                          tgacccTGAGCCCGCCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGAg                                     >  7:114552/5‑125 (MQ=255)                                                                                                                                            acccTGAGCCCGCCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGAGGGTACCGCCGTCGGCACTGAAcag             >  5:111604/3‑147 (MQ=255)                                                                                                                                             tCCTGTGGCGGGCTCAGTGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGAGGGTACCGCCGTCTGCACTGAAaagca           <  4:35133/148‑1 (MQ=255)                                                                                                                                             tCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGAGGGTACCGCCGTCGGCACTGAAcagcag          >  1:325900/1‑149 (MQ=255)                                                                                                                                                  aGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGAGGGTACCGCCGTCGGCACTGAACAGCAGGTTGc     >  2:104916/1‑149 (MQ=255)                                                                                                                                                   ggCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGAGGGTACCGCAGTCGGCACTGAACAGCAGGTTGcc    <  5:150514/149‑1 (MQ=255)                                                                                                                                                      gggCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGAGGGTACCGCCGTCGGCACTGAACAGCAGGTTGCCgg  >  1:146407/1‑148 (MQ=255)                                                                                                                                                      |                                                                                                                                                    CAGGTTGACTGTGGCCGTGGCCAGGTTGTCGCTGGCGATGACCGGCGCGTTGGCATCCACATCCGGTGCAGTGACGTTGACGTTGCCCGACACGTTGCCGCGCGGG‑TCGCTGAGGGTCACCGAAAGGATCTGGCCGTTGTCCTGAGGCGGGCTCAGGGTCACGGTGAAGGTGCCGTCCGCCTGCACCGTCGCGGTCTGCAGCACGGTACCGCCGGCGCTGCGTACGGTGACGGTGGCACCCACTTCGCCAGTACCGGTGAGGGTACCGCCGTCGGCACTGAACAGCAGGTTGCCGG  >  NC_002947/928705‑929000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑