Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	936,221	G→C	6.1%	A3134G (GCC→GGC)	PP_0806 ←	surface adhesion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	936,221	0	G	C	6.1%	267.8 / 4.5	82	A3134G (GCC→GGC)	PP_0806	surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base G (41/36);  new base C (2/3);  total (43/39)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01

CGGTGAGCAGGCTGCCATCGGCACTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTGCGGTACCGGAAACGCTGGAGCCGTCACCGGCCACCACCACGCC  >  NC_002947/936075‑936366                                                                                                                                                   |                                                                                                                                                   cGGTGAGCAGGCTGCCATCGGCACTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCTAACTGTTGCCAGCGCCGTCAACAGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGcc                                                                                                                                                  >  7:205829/1‑149 (MQ=255) cGGTGAGCAGGCTGCCATCGGCACTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGcc                                                                                                                                                  >  8:116024/1‑149 (MQ=255) cGGTGAGCAGGCTGCCATCGGCACTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGcc                                                                                                                                                  <  1:267776/149‑1 (MQ=255)  ggTGAGCAGGCTGCCATCGGCACTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCg                                                                                                                                                 <  5:175979/149‑1 (MQ=255)   gTGAGCAGGCTGCCATCGGCACTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCgg                                                                                                                                                >  4:104601/1‑149 (MQ=255)   gTGAGCAGGCTGCCATCGGCACTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCgg                                                                                                                                                <  8:182197/149‑1 (MQ=255)        cAGGCTGCCATCGGCACTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCACCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGt                                                                                                                                             >  8:239031/1‑147 (MQ=255)         aGGCTGCCATCGGCACTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGCCATACAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAg                                                                                                                                          >  2:270949/1‑149 (MQ=255)           gCTGCCATCGGCACTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGc                                                                                                                                        >  2:139773/1‑149 (MQ=255)           gCTGCCATCGGCACTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGc                                                                                                                                        >  4:169162/1‑149 (MQ=255)                cATCGGCACTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑Cg                                                                                                                                  >  3:147336/1‑149 (MQ=255)                cATCGGCACTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTCGGCCTGACCCCGGCCCAGGCCAA‑Cg                                                                                                                                  <  1:4908/149‑1 (MQ=255)                  tCGGCACTCAGTTGCAGTTCGCTGGCGTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGt                                                                                                                                <  1:199216/149‑1 (MQ=255)                     gCACTCAGTTGCAGTTCGTTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTg                                                                                                                               >  2:336381/1‑147 (MQ=255)                     gCACTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTg                                                                                                                               >  5:152795/1‑147 (MQ=255)                     gCACTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTg                                                                                                                               >  1:310369/1‑147 (MQ=255)                     gCACTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGATAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGACCCCGGCCCAGGCCAA‑CGGCGag                                                                                                                             <  6:170334/149‑2 (MQ=255)                      cACTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAg                                                                                                                            >  6:272762/1‑149 (MQ=255)                       aCTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGc                                                                                                                           >  4:301035/1‑149 (MQ=255)                           aGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTg                                                                                                                         <  5:150734/147‑1 (MQ=255)                           aGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGAGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCggg                                                                                                                                               >  1:364779/1‑126 (MQ=255)                             ttGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCAt                                                                                                                     >  6:190048/1‑149 (MQ=255)                              tGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATc                                                                                                                    >  6:253016/1‑149 (MQ=255)                                 aGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCg                                                                                                                  >  1:232720/1‑148 (MQ=255)                                  gTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGa                                                                                                                >  8:66577/1‑149 (MQ=255)                                     cGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCg                                                                                                             >  1:325443/1‑149 (MQ=255)                                         ggCTTCATTGGTCGGTGAGATATCGGGGGCGGTGATCTGGTTAGGCAGCTAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGTGTCTCGCCGTTGGCCTGGGCCGTGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCt                                                                                                         <  8:205829/149‑1 (MQ=255)                                           ctgcATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTgg                                                                                                       <  8:25698/146‑1 (MQ=255)                                           cTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCACCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTgg                                                                                                       <  7:239031/149‑1 (MQ=255)                                            ttCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTggg                                                                                                      >  3:200149/1‑149 (MQ=255)                                            ttCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTggg                                                                                                      >  7:278787/1‑149 (MQ=255)                                                 tGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCt                                                                                                  <  4:147336/148‑1 (MQ=255)                                                  gggCGGGGTTATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTg                                                                                                 <  7:347590/148‑1 (MQ=255)                                                     cgggtgcatatCGGGGGCGGTGATCTGGGTAGGCAGCTAACTGTTGCCAGCGCCGTCAACCTCCACGACATCCAGGGGCTCGCCGTTGGCGTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCa                                                                                             <  5:33945/142‑1 (MQ=255)                                                       gggTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAgg                                                                                           <  1:139773/149‑1 (MQ=255)                                                        ggTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCAGCGCCCAggc                                                                                          <  7:116024/149‑2 (MQ=255)                                                        ggTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCAGCGCCCAggc                                                                                          <  5:272762/149‑2 (MQ=255)                                                        ggTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTCGGCCTGACCCCGGCCCAGGCCAA‑Cgg                                                                                                                                 >  7:129000/1‑110 (MQ=38)                                                        ggTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTCGGCCTGACCCCGGCCCAGGCCAA‑Cgg                                                                                                                                 <  8:129000/110‑1 (MQ=38)                                                        ggTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAACGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAgg                                                                                           >  4:20400/1‑148 (MQ=255)                                                        ggTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAACGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAgg                                                                                           <  3:20400/148‑1 (MQ=255)                                                         gTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGAc                                                                                         <  5:253016/149‑1 (MQ=255)                                                          tGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACa                                                                                        >  2:47343/1‑149 (MQ=255)                                                          tGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACa                                                                                        >  2:184909/1‑149 (MQ=255)                                                            atatCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACAtt                                                                                      >  8:62950/1‑149 (MQ=255)                                                               tCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGcc                                                                                   <  2:275436/149‑1 (MQ=255)                                                                   gggCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTgc                                                                               <  1:47343/149‑1 (MQ=255)                                                                    ggCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGACCTGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTgc                                                                               <  1:280251/149‑1 (MQ=255)                                                                    ggCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTgcg                                                                              <  2:325443/149‑1 (MQ=255)                                                                         tGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCa                                                                         <  3:119639/149‑1 (MQ=255)                                                                             cTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCa                                                                     >  7:201550/1‑149 (MQ=255)                                                                                ggTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACg                                                                       <  3:169162/144‑1 (MQ=255)                                                                                ggTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGc                                                                      <  3:104601/145‑1 (MQ=255)                                                                                ggTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCa                                                                     <  8:278787/146‑1 (MQ=255)                                                                                 gTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTc                                                                 <  6:152795/149‑1 (MQ=255)                                                                                      cAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCt                                                            <  1:184909/149‑1 (MQ=255)                                                                                        gCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTgg                                                          <  5:190048/149‑1 (MQ=255)                                                                                        gCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTgg                                                          <  5:176723/149‑1 (MQ=255)                                                                                        gCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTgg                                                          <  7:62950/149‑1 (MQ=255)                                                                                              tGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCt                                                    >  5:87567/1‑149 (MQ=255)                                                                                              tGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCt                                                    <  7:66577/149‑1 (MQ=255)                                                                                                   ccAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCgg                                               >  3:264208/1‑149 (MQ=255)                                                                                                   ccAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCg                                                <  1:336381/148‑1 (MQ=255)                                                                                                    cAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTgcg                                                                              >  2:39739/1‑117 (MQ=255)                                                                                                    cAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTgcg                                                                              <  1:39739/117‑1 (MQ=255)                                                                                                    cAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGt                                              >  7:338054/1‑149 (MQ=255)                                                                                                     aGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTg                                             >  4:152655/1‑149 (MQ=255)                                                                                                            tCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGAGGCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTGCGGTAcc                                      <  6:121956/149‑1 (MQ=255)                                                                                                              aaCCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACGTGGCTATCTGCCTCTCCGGGACCg                                     >  8:98181/1‑148 (MQ=255)                                                                                                                ccGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTGCGGTACCGGa                                   >  5:19510/1‑148 (MQ=255)                                                                                                                      cGACATCCAGGGTCTCGCCGTTGTCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTGCGGTACCGGAAACGCTg                            <  1:270949/149‑1 (MQ=255)                                                                                                                          aTCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTGCGGTACCGGAAACGCTGGAGc                        <  8:201550/149‑1 (MQ=255)                                                                                                                           tCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTGCGGTACCGGAAACGCTGGAGcc                       >  6:353660/1‑149 (MQ=255)                                                                                                                             cAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTGCGGTACCGGAAACGCTGGAGCCGt                     >  5:33900/1‑149 (MQ=255)                                                                                                                              aGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTGCGGTACCGGAAACGCTGGAGCCGt                     >  1:336901/1‑148 (MQ=255)                                                                                                                                ggTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTGCGGTACCGGAAACGCTGGAGCCGTCAc                  >  8:106811/1‑149 (MQ=255)                                                                                                                                      gCCGTTGGCCTGGGCTGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTGCGGTACCGGAAACGCTGGAGCCGTCACCGGcc             <  8:338054/148‑1 (MQ=255)                                                                                                                                      gCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTGCGGTACCGGAAACGCTGGAGCCGTCACCGGcca            >  2:366725/1‑149 (MQ=255)                                                                                                                                      gCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTGCGGTACCGGAAACGCTGGAGCCGTCACCGGcca            >  6:153041/1‑149 (MQ=255)                                                                                                                                        cGTCGGCCTGACCCCGGCCCAGGCCAA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTGCGGTACCGGAAACGCTGGAGCCGTCACCGGccac           >  1:326761/1‑148 (MQ=255)                                                                                                                                         gTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTGCGGTACCGGAAACGCTGGAGCCGTCACCGGccacca         >  2:268903/1‑149 (MQ=255)                                                                                                                                                tGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTGCGGTACCGGAAACGCTGGAGCCGTCACCGGCCACCACCAcgcc  >  7:26858/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CGGTGAGCAGGCTGCCATCGGCACTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTGCGGTACCGGAAACGCTGGAGCCGTCACCGGCCACCACCACGCC  >  NC_002947/936075‑936366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑