Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	936,224	G→C	6.6%	P3133R (CCG→CGG)	PP_0806 ←	surface adhesion protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	936,224	0	G	C	6.6%	246.5 / 4.6	76	P3133R (CCG→CGG)	PP_0806	surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base G (38/33);  new base C (2/3);  total (40/36)
Fisher's exact test for biased strand distribution p-value = 6.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

GGTGAGCAGGCTGCCATCGGCACTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTGCGGTACCGGAAACGCTGGAGCCGTCACCGGCCACCACCACGCCGCTCG  >  NC_002947/936076‑936371                                                                                                                                                     |                                                                                                                                                     ggTGAGCAGGCTGCCATCGGCACTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCg                                                                                                                                                      <  5:175979/149‑1 (MQ=255)  gTGAGCAGGCTGCCATCGGCACTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCgg                                                                                                                                                     <  8:182197/149‑1 (MQ=255)  gTGAGCAGGCTGCCATCGGCACTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCgg                                                                                                                                                     >  4:104601/1‑149 (MQ=255)       cAGGCTGCCATCGGCACTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCACCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGt                                                                                                                                                  >  8:239031/1‑147 (MQ=255)        aGGCTGCCATCGGCACTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGCCATACAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAg                                                                                                                                               >  2:270949/1‑149 (MQ=255)          gCTGCCATCGGCACTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGc                                                                                                                                             >  4:169162/1‑149 (MQ=255)          gCTGCCATCGGCACTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGc                                                                                                                                             >  2:139773/1‑149 (MQ=255)               cATCGGCACTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑Cg                                                                                                                                       >  3:147336/1‑149 (MQ=255)               cATCGGCACTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTCGGCCTGACCCCGGCCCAGGCCAA‑Cg                                                                                                                                       <  1:4908/149‑1 (MQ=255)                 tCGGCACTCAGTTGCAGTTCGCTGGCGTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGt                                                                                                                                     <  1:199216/149‑1 (MQ=255)                    gCACTCAGTTGCAGTTCGTTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTg                                                                                                                                    >  2:336381/1‑147 (MQ=255)                    gCACTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTg                                                                                                                                    >  1:310369/1‑147 (MQ=255)                    gCACTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTg                                                                                                                                    >  5:152795/1‑147 (MQ=255)                    gCACTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGATAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGACCCCGGCCCAGGCCAA‑CGGCGag                                                                                                                                  <  6:170334/149‑2 (MQ=255)                     cACTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAg                                                                                                                                 >  6:272762/1‑149 (MQ=255)                      aCTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGc                                                                                                                                >  4:301035/1‑149 (MQ=255)                          aGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTg                                                                                                                              <  5:150734/147‑1 (MQ=255)                          aGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGAGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCggg                                                                                                                                                    >  1:364779/1‑126 (MQ=255)                            ttGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCAt                                                                                                                          >  6:190048/1‑149 (MQ=255)                             tGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATc                                                                                                                         >  6:253016/1‑149 (MQ=255)                                aGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCg                                                                                                                       >  1:232720/1‑148 (MQ=255)                                 gTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGa                                                                                                                     >  8:66577/1‑149 (MQ=255)                                    cGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCg                                                                                                                  >  1:325443/1‑149 (MQ=255)                                        ggCTTCATTGGTCGGTGAGATATCGGGGGCGGTGATCTGGTTAGGCAGCTAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGTGTCTCGCCGTTGGCCTGGGCCGTGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCt                                                                                                              <  8:205829/149‑1 (MQ=255)                                          ctgcATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTgg                                                                                                            <  8:25698/146‑1 (MQ=255)                                          cTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCACCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTgg                                                                                                            <  7:239031/149‑1 (MQ=255)                                           ttCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTggg                                                                                                           >  7:278787/1‑149 (MQ=255)                                           ttCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTggg                                                                                                           >  3:200149/1‑149 (MQ=255)                                                tGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCt                                                                                                       <  4:147336/148‑1 (MQ=255)                                                 gggCGGGGTTATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTg                                                                                                      <  7:347590/148‑1 (MQ=255)                                                    cgggtgcatatCGGGGGCGGTGATCTGGGTAGGCAGCTAACTGTTGCCAGCGCCGTCAACCTCCACGACATCCAGGGGCTCGCCGTTGGCGTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCa                                                                                                  <  5:33945/142‑1 (MQ=255)                                                      gggTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAgg                                                                                                <  1:139773/149‑1 (MQ=255)                                                       ggTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCAGCGCCCAggc                                                                                               <  7:116024/149‑2 (MQ=255)                                                       ggTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCAGCGCCCAggc                                                                                               <  5:272762/149‑2 (MQ=255)                                                       ggTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTCGGCCTGACCCCGGCCCAGGCCAA‑Cgg                                                                                                                                      <  8:129000/110‑1 (MQ=38)                                                       ggTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTCGGCCTGACCCCGGCCCAGGCCAA‑Cgg                                                                                                                                      >  7:129000/1‑110 (MQ=38)                                                       ggTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAACGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAgg                                                                                                <  3:20400/148‑1 (MQ=255)                                                       ggTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAACGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAgg                                                                                                >  4:20400/1‑148 (MQ=255)                                                        gTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGAc                                                                                              <  5:253016/149‑1 (MQ=255)                                                         tGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACa                                                                                             >  2:47343/1‑149 (MQ=255)                                                         tGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACa                                                                                             >  2:184909/1‑149 (MQ=255)                                                           atatCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACAtt                                                                                           >  8:62950/1‑149 (MQ=255)                                                              tCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGcc                                                                                        <  2:275436/149‑1 (MQ=255)                                                                  gggCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTgc                                                                                    <  1:47343/149‑1 (MQ=255)                                                                   ggCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGACCTGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTgc                                                                                    <  1:280251/149‑1 (MQ=255)                                                                   ggCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTgcg                                                                                   <  2:325443/149‑1 (MQ=255)                                                                        tGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCa                                                                              <  3:119639/149‑1 (MQ=255)                                                                            cTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCa                                                                          >  7:201550/1‑149 (MQ=255)                                                                               ggTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACg                                                                            <  3:169162/144‑1 (MQ=255)                                                                               ggTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGc                                                                           <  3:104601/145‑1 (MQ=255)                                                                               ggTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCa                                                                          <  8:278787/146‑1 (MQ=255)                                                                                gTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTc                                                                      <  6:152795/149‑1 (MQ=255)                                                                                     cAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCt                                                                 <  1:184909/149‑1 (MQ=255)                                                                                       gCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTgg                                                               <  7:62950/149‑1 (MQ=255)                                                                                       gCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTgg                                                               <  5:190048/149‑1 (MQ=255)                                                                                       gCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTgg                                                               <  5:176723/149‑1 (MQ=255)                                                                                             tGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCt                                                         >  5:87567/1‑149 (MQ=255)                                                                                             tGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCt                                                         <  7:66577/149‑1 (MQ=255)                                                                                                  ccAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCgg                                                    >  3:264208/1‑149 (MQ=255)                                                                                                  ccAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCg                                                     <  1:336381/148‑1 (MQ=255)                                                                                                   cAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTgcg                                                                                   <  1:39739/117‑1 (MQ=255)                                                                                                   cAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTgcg                                                                                   >  2:39739/1‑117 (MQ=255)                                                                                                   cAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGt                                                   >  7:338054/1‑149 (MQ=255)                                                                                                    aGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTg                                                  >  4:152655/1‑149 (MQ=255)                                                                                                           tCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGAGGCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTGCGGTAcc                                           <  6:121956/149‑1 (MQ=255)                                                                                                             aaCCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACGTGGCTATCTGCCTCTCCGGGACCg                                          >  8:98181/1‑148 (MQ=255)                                                                                                               ccGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTGCGGTACCGGa                                        >  5:19510/1‑148 (MQ=255)                                                                                                                     cGACATCCAGGGTCTCGCCGTTGTCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTGCGGTACCGGAAACGCTg                                 <  1:270949/149‑1 (MQ=255)                                                                                                                         aTCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTGCGGTACCGGAAACGCTGGAGc                             <  8:201550/149‑1 (MQ=255)                                                                                                                          tCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTGCGGTACCGGAAACGCTGGAGcc                            >  6:353660/1‑149 (MQ=255)                                                                                                                            cAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTGCGGTACCGGAAACGCTGGAGCCGt                          >  5:33900/1‑149 (MQ=255)                                                                                                                             aGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTGCGGTACCGGAAACGCTGGAGCCGt                          >  1:336901/1‑148 (MQ=255)                                                                                                                               ggTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTGCGGTACCGGAAACGCTGGAGCCGTCAc                       >  8:106811/1‑149 (MQ=255)                                                                                                                                     gCCGTTGGCCTGGGCTGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTGCGGTACCGGAAACGCTGGAGCCGTCACCGGcc                  <  8:338054/148‑1 (MQ=255)                                                                                                                                     gCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTGCGGTACCGGAAACGCTGGAGCCGTCACCGGcca                 >  2:366725/1‑149 (MQ=255)                                                                                                                                     gCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTGCGGTACCGGAAACGCTGGAGCCGTCACCGGcca                 >  6:153041/1‑149 (MQ=255)                                                                                                                                       cGTCGGCCTGACCCCGGCCCAGGCCAA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTGCGGTACCGGAAACGCTGGAGCCGTCACCGGccac                >  1:326761/1‑148 (MQ=255)                                                                                                                                        gTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTGCGGTACCGGAAACGCTGGAGCCGTCACCGGccacca              >  2:268903/1‑149 (MQ=255)                                                                                                                                               tGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTGCGGTACCGGAAACGCTGGAGCCGTCACCGGCCACCACCAcgcc       >  7:26858/1‑149 (MQ=255)                                                                                                                                                    cGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTGCGGTACCGGAAACGCTGGAGCCGTCACCGGCCACCACCACGCCGCTCg  >  6:356483/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                     GGTGAGCAGGCTGCCATCGGCACTCAGTTGCAGTTCGCTGGCTTCATTGGGCGGGGTGATATCGGGGGCGGTGATCTGGGTAGGCAGCGAACTGTTGCCAGCGCCGTCAACCGCCACGACATCCAGGGTCTCGCCGTTGGCCTGGGCCGGGGTCAGGCCGA‑CGGTGAAGCTGCCATCCGGACCGGCCTGGGCGCTGCCCAGGACATTGCCCTGCGCGTCACGCACTTCCACCTGGGTATCTGCCGGTGCGGTACCGGAAACGCTGGAGCCGTCACCGGCCACCACCACGCCGCTCG  >  NC_002947/936076‑936371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑