Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 959,450 T→C 5.8% F85F (TTT→TTC)  PP_5460 → membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947959,4500TC 5.8% 209.3 / 2.2 69F85F (TTT→TTC) PP_5460membrane protein
Reads supporting (aligned to +/- strand):  ref base T (36/29);  new base C (2/2);  total (38/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01

CTGTACGGGCCATGGGGCATGCCGTTCGGCTTGTACCAATGGTTTGCCAATCCGCTGCTGGCCCTGGCCATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTGGCTGGTAGCGATGGTGGTGTTTTGCCTGGGGCAAGCCTGGTTTTGCTGGAAGGCGCGAAGGGC  >  NC_002947/959304‑959590
                                                                                                                                                  |                                                                                                                                             
ctgTACGGGCCATGGGGCATGCCGTTCGGCTTGTACCAATGGTTTGCCAATCCGCTGCTGGCCCTGGCCATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGtttt                                                                                                                                               <  7:68537/147‑1 (MQ=255)
  gTACGGGCCATGGGGCATGCCGTTCGGCTTGTACCAATGGTTTGCCAATCCGCTGCTGGCCCTGGCCATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTg                                                                                                                                              >  6:105288/1‑146 (MQ=255)
  gTACGGGCCATGGGGCATGCCGTTCGGCTTGTACCAATGGTTTGCCAATCCGCTGCTGGCCCTGGCCATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTg                                                                                                                                              <  5:105288/146‑1 (MQ=255)
  gTACGGGCCATGGGGCATGCCGTTCGGCTTGTACCAATGGTTTGCCAATCCGCTGCTGGCCCTGGCCATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCa                                                                                                                                           >  5:300562/1‑149 (MQ=255)
   tACGGGCCATGGGGCATGCCGTTCGGCTTGTACCAATGGTTTGCCAATCCGCTGCTGGCCCTGGCCATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCAt                                                                                                                                          >  3:148856/1‑149 (MQ=255)
     cGGGCCATGGGGCATGCCGTTCGGCTTGTACCAATGGTTTGCCAATCCGCTGCTGGCCCTGGCCATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATc                                                                                                                                         >  8:138983/1‑148 (MQ=255)
         ccATGGGGCATGCCGTTCGGCTTGTACCAATGGTTTGCCAATCCGCTGCTGGCCCTGGCCATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCg                                                                                                                                    >  5:338863/1‑149 (MQ=255)
                gCATGCCGTTCGGCTTGTACCAATGGTTTGCCAATCCGCTGCTGGCCCTGGCCATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGTGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCg                                                                                                                             >  4:59913/1‑149 (MQ=255)
                   tGCCGTTCGGCTTGTACCAATGGTTTGCCAATCCGCTGCTGGCCCTGGCCATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGAt                                                                                                                          <  7:138983/149‑1 (MQ=255)
                      cGTTCGGCTTGTACCAATGGTTTGCCAATCCGCTGCTGGCCCTGGCCATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAAc                                                                                                                       <  8:201722/149‑1 (MQ=255)
                             cTTGTACCAATGGTTTGCCAATCCGCTGCTGGCCCTGGCCATCCTGGCTCATCGGCGGTTTAGCCGGCTGGCCTTGCTGGCCGGGCGTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCt                                                                                                                >  2:207458/1‑149 (MQ=255)
                                gTACCAATGGTTTGCCAATCCGCTGCTGGCCCTGGCCATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTAt                                                                                                              >  2:96976/1‑148 (MQ=255)
                                 tACCAATGGTTTGCCAATCCGCTGCTGGCCCTGGCCATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGa                                                                                                            <  8:232134/149‑1 (MQ=255)
                                   ccAATGGTTTGCCAATCCGCTGCTGGCCCTGGCCATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGt                                                                                                          <  2:114521/149‑1 (MQ=255)
                                         gTTTGCCAATCCGCTGCTGGCCCTGGCCATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCAt                                                                                                     <  6:338863/148‑1 (MQ=255)
                                         gTTTGCCAATCCGCTGCTGGCCCTGGCCATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTCGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCAt                                                                                                     >  1:264717/1‑148 (MQ=255)
                                         gTTTGCCAATCCGCTGCTGGCCCTGGCCATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTCGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATg                                                                                                    >  1:259579/1‑149 (MQ=255)
                                          tttGCCAATCCGCTGCTGGCCCTGGCCATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGa                                                                                                   <  8:158836/149‑1 (MQ=255)
                                          tttGCCAATCCGCTGCTGGCCCTGGCCATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGa                                                                                                   >  2:314858/1‑149 (MQ=255)
                                               cAATCCGCTGCTGGCCCTGGCCATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTGTGAGTTTCATGAC‑CTTa                                                                                             >  3:220487/1‑149 (MQ=255)
                                               cAATCCGCTGCTGGCCCTGGCCATCCTGGCTCAACGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCCGATAACAGCAGCTATGAGTTTCATGAC‑CTTa                                                                                             >  5:300691/1‑149 (MQ=255)
                                                aaTCCGCTGCTGGCCCTGGCCATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGCGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTAc                                                                                            >  2:142261/1‑149 (MQ=255)
                                                 aTCCGCTGCTGGCCCTGGCCATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTAcc                                                                                           >  2:269748/1‑149 (MQ=255)
                                                   ccGCTGCTGGCCCTGGCCATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAAc                                                                                                                       <  2:91704/120‑1 (MQ=255)
                                                   ccGCTGCTGGCCCTGGCCATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCATTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAAc                                                                                                                       >  1:91704/1‑120 (MQ=255)
                                                    cGCTGCTGGCCCTGGCCATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGc                                                                                        <  6:300562/149‑1 (MQ=255)
                                                     gctgctGGCCCTGGCCATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGtttt                                                                                                                                               >  5:311834/1‑94 (MQ=255)
                                                     gctgctGGCCCTGGCCATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGtttt                                                                                                                                               <  6:311834/94‑1 (MQ=255)
                                                     gctgctGGCCCTGGCCATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCt                                                                                       >  1:75571/1‑149 (MQ=255)
                                                      ctgctgGCCCTGGCCATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCtt                                                                                      <  4:130780/149‑1 (MQ=255)
                                                         ctggccctggccATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTg                                                                                    <  2:40898/148‑1 (MQ=255)
                                                               ctggccATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTgg                                                                             <  2:75571/149‑1 (MQ=255)
                                                                tggccATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCt                                                                               >  3:71135/1‑146 (MQ=255)
                                                                tggccATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCt                                                                               <  4:71135/146‑1 (MQ=255)
                                                                tggccATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGc                                                                            >  8:51192/1‑149 (MQ=255)
                                                                    cATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTc                                                                         >  2:352213/1‑148 (MQ=255)
                                                                        cTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGACACTTACCGGCTTTGGTGCTGGCTTCTAc                                                                      <  1:207458/148‑1 (MQ=255)
                                                                            cTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTgg                                                                >  5:266711/1‑149 (MQ=255)
                                                                             tCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTGGc                                                               >  7:213345/1‑149 (MQ=255)
                                                                                tCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTGGCTgg                                                            <  1:314858/149‑1 (MQ=255)
                                                                                tCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTCGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTGGCTgg                                                            <  2:264717/149‑1 (MQ=255)
                                                                                tCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTCGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTGGCTgg                                                            <  2:259579/149‑1 (MQ=255)
                                                                                 cggcggTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTGGCTgg                                                            >  3:162001/1‑148 (MQ=255)
                                                                                  ggcggTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTGGCTgg                                                            >  4:151778/1‑147 (MQ=255)
                                                                                  ggcggTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTGGCTGGTa                                                          <  3:59913/149‑1 (MQ=255)
                                                                                  ggcggTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTGGCTGGTa                                                          <  5:53466/149‑1 (MQ=255)
                                                                                       tttCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTGGCTGGTAGCGAt                                                     >  8:48846/1‑149 (MQ=255)
                                                                                           gCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTTTGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTGGCTGGTAGCGAtggtg                                                 >  7:141193/1‑149 (MQ=255)
                                                                                           gCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTGGCTGGTAGCGAtggtg                                                 <  4:148856/149‑1 (MQ=255)
                                                                                           gCCGGCTGGACTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTGGCTGGTAGCGAtggtg                                                 >  4:114008/1‑149 (MQ=255)
                                                                                            ccGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTTTGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTGGCTGGTAGGGAtggtgg                                                >  8:54107/1‑149 (MQ=255)
                                                                                               gCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCAGCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTGGCTGGTAGCGATGGTGgtgt                                             <  2:102680/149‑1 (MQ=255)
                                                                                                 tGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTGGCTGGTAGCGATGGTGGTGttt                                           >  5:210762/1‑149 (MQ=255)
                                                                                                      ttGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTGGCTGGTAGCGATGGTGGTGTTTTGCCt                                      >  5:201174/1‑149 (MQ=255)
                                                                                                        gCTTGCCGTGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCTTCGACCGCTTGCCGGATATCAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTGGCTGGTAGCGATGGTGGTGTTTTGCCTg                                     <  6:300691/148‑1 (MQ=255)
                                                                                                              cGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTGGCTGGTAGCGATGGTGGTGTTTTGCCTGGGGCAAg                              >  1:332283/1‑149 (MQ=255)
                                                                                                                       ggcggcGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTGGCTGGTAGCGATTGTGGTGTTTTGCCTGGGGCAAGCCTGGtttt                     <  3:347440/149‑1 (MQ=255)
                                                                                                                         cggcgTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTGGCTGGTAGCGATGGTGGTGTTTTGCCTGGGGCAAGCCTGGTTTTGc                   <  7:48846/149‑1 (MQ=255)
                                                                                                                          ggcgTATCTTGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTTCCTGTGGCTGGTAGCGATGGGGGTGTTTTGCCTGGGGCAAGCCTGGTTTTGCt                  <  6:80551/149‑1 (MQ=255)
                                                                                                                           gcgTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTGGCTGGTAGCGATGGTGGTGTTTTGCCTGGGGCAAGCCTGGTTTTGCTg                 >  1:170224/1‑149 (MQ=255)
                                                                                                                            cgTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTGGCTGGTAGCGATGGTGGTGTTTTGCCTGGGGCAAGCCTGGTTTTGCTgg                <  1:269748/149‑1 (MQ=255)
                                                                                                                              tATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTGGCTGGTAGCGATGGTGGTGTTTTGCCTGGGGCAAGCCTGGTTTTGCTgg                >  2:12409/1‑147 (MQ=255)
                                                                                                                              tATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTGGCTGGTAGCGATGGTGGTGTTTTGCCTGGGGCAAGCCTGGTTTTGCTGGaa              <  3:114008/149‑1 (MQ=255)
                                                                                                                                  tGGCTGCCAGCAGTTTTGGCATCGACCGCTTGACGGATAACAGCAGATATGAGTTTCATTAC‑CTTACCGTCTTTTGTGCTGGCTTCTACCTGTTGCTGGTAGCGATGGTGGTGTTTTGCCTTGGGCAAGACTGGTTTTGCTGGAAGgcg          >  6:140254/1‑149 (MQ=255)
                                                                                                                                    gCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTGGCTGGTAGCGATGGTGGTGTTTTGCCTGGGGCAAGCCTGGTTTTGCTGGAAGgcgc         <  2:170224/148‑1 (MQ=255)
                                                                                                                                    gCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTGGCTGGTAGCGATGGTGGTGTTTTGCCTGGGGCAAGCCTGGTTTTGCTGGAAGgcgc         >  8:3742/1‑148 (MQ=255)
                                                                                                                                     cTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTTTGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTGGCTGGTAGCGATGGTGGTGTTTTGCCTGGGGCAAGCCTGGTTTTGCTGGAAGGCGCGa       <  8:141193/149‑1 (MQ=255)
                                                                                                                                      tGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTGGCTGGTAGCGATGGTGGTGTTTTGCCTGGGGCAAGCCTGGTTTTGCTGGAAGGcgcg        >  4:341396/1‑147 (MQ=255)
                                                                                                                                      tGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTGGCTGGTAGCGATGGTGGTGTTTTGCCTGGGGCAAGCCTGGTTTTGCTGGAAGGcgcg        >  6:154708/1‑147 (MQ=255)
                                                                                                                                          agcagTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTGGCTGGTAGCGATGGTGGTGTTTTGCCTGGGGCAAGCCTGGTTTTGCTGGAAGGCGCGAAGGGc  >  4:261237/1‑149 (MQ=255)
                                                                                                                                          agcagTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTGGCTGGTAGCGATGGTGGTGTTTTGCCTGGGGCAAGCCTGGTTTTGCTGGAAGGCGCGAAGGGc  <  3:341396/149‑1 (MQ=255)
                                                                                                                                          agcagTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTGGCTGGTAGCGATGGTGGTGTTTTGCCTGGGGCAAGCCTGGTTTTGCTGGAAGGCGCGAAGGGc  <  5:154708/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                             
CTGTACGGGCCATGGGGCATGCCGTTCGGCTTGTACCAATGGTTTGCCAATCCGCTGCTGGCCCTGGCCATCCTGGCTCATCGGCGGTTTCGCCGGCTGGCCTTGCTGGCCGGGCTTGGGGCGGCGTATCTGGCTGCCAGCAGTTTTGGCATCGACCGCTTGCCGGATAACAGCAGCTATGAGTTTCATGAC‑CTTACCGGCTTTGGTGCTGGCTTCTACCTGTGGCTGGTAGCGATGGTGGTGTTTTGCCTGGGGCAAGCCTGGTTTTGCTGGAAGGCGCGAAGGGC  >  NC_002947/959304‑959590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑