Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,013,249 T→G 15.9% intergenic (+77/+54) prfC → / ← potF‑I peptide chain release factor 3/putrescine‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,013,2490TG15.9% 103.0 / 13.9 50intergenic (+77/+54)prfC/potF‑Ipeptide chain release factor 3/putrescine‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (25/17);  new base G (5/3);  total (30/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.33e-01

GGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATCACCT  >  NC_002947/1013102‑1013397
                                                                                                                                                   |                                                                                                                                                    
gggTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAtg                                                                                                                                                     <  1:281823/149‑1 (MQ=255)
gggTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAtg                                                                                                                                                     <  8:219235/149‑1 (MQ=255)
 gttCAACCTGGCGCTGATGGAAGAGCTCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGATATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCAGCAGACCTGGAGGTCAtgt                                                                                                                                                    <  7:148534/147‑1 (MQ=255)
 ggTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAtgt                                                                                                                                                    <  8:44382/149‑1 (MQ=255)
   tCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGa                                                                                                                                                  <  1:226383/149‑1 (MQ=255)
           gcgcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCtgt                                                                                                                                           <  3:111984/148‑1 (MQ=255)
             gcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTggg                                                                                                                                        >  8:270453/1‑149 (MQ=255)
              cTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCCGCCTAGCGGCCCTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGa                                                                                                                                       <  7:54343/149‑1 (MQ=17)
                                 cGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCCCTCCCCCAGGAATAACCAGACCTGGAGGTGATGTGATACCTGTGGGGGCGGGCGGGCCCGCGCAgg                                                                                                                    >  1:336489/1‑149 (MQ=38)
                                    aCATCAAGTTCCGCGCTACCCGCGGGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGcc                                                                                                                 >  5:172787/1‑149 (MQ=255)
                                      aTCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGc                                                                                                               <  4:66882/149‑1 (MQ=255)
                                        cAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCGGTGGGAGCGGGCGTGCCCGCGAAGGGCCGc                                                                                                               >  8:35472/1‑147 (MQ=255)
                                        cAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTCGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGc                                                                                                               >  6:124196/1‑147 (MQ=255)
                                             tCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTCCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAggcgg                                                                                                        >  5:177671/1‑149 (MQ=255)
                                               cgcgCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGcc                                                                                                      >  5:369471/1‑149 (MQ=255)
                                                           gAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAAttt                                                                                          >  7:254157/1‑149 (MQ=255)
                                                             gCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTcc                                                                                        >  5:270663/1‑149 (MQ=255)
                                                               aCCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCAACCACACGAATCACCAGACCTGGAGGTCATGGGGTTACAGTGGGAGCGGGCCGGGCCGCCAAGGGGCCCGTGGCGCGCCCAGTTCAAtttaacg                                                                                      >  4:250425/1‑145 (MQ=19)
                                                                ccATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGc                                                                                     >  5:74047/1‑149 (MQ=255)
                                                                 cATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACACGAATAACCCGACCTGGAGGTCATGTGATAACTGTGGGAGCGGGCGTGCCCGCGCAGGGCCGCGAGGCGGCCCCATTTCAATTTGCgggt                                                                                    >  8:18369/1‑147 (MQ=25)
                                                                  aTTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCtt                                                                                   <  6:74047/149‑1 (MQ=255)
                                                                  aTTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCtt                                                                                   >  1:253207/1‑149 (MQ=255)
                                                                   ttAAGGCCTCAATACGGGGCTGCTTTGCAGCCCGTCGCGGGCACGCCCGCTCCCACCGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCttt                                                                                  <  3:250425/149‑1 (MQ=37)
                                                                        gCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATAc                                                                             >  8:193005/1‑149 (MQ=255)
                                                                                 cGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATAGCTGTGGGAGCGGGCGGGGCCGCGAAGGGCCGCTAGGCGGCGCCATTTGAATTTACGGCTTTGATAATGGTc                                                                        >  4:119472/1‑145 (MQ=37)
                                                                                    ggcggcTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGTGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTc                                                                 <  3:119472/149‑1 (MQ=255)
                                                                                      ctgctTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTcgc                                                               <  3:302486/149‑1 (MQ=255)
                                                                                      ctgctTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTcgc                                                               <  7:270453/149‑1 (MQ=255)
                                                                                            tGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTTTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTAc                                                           <  2:253207/147‑1 (MQ=255)
                                                                                                     tCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCg                                                <  6:270663/149‑1 (MQ=255)
                                                                                                      cgcgGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTc                                                 <  6:153522/147‑1 (MQ=255)
                                                                                                       gcgGGCACGCCCGCTCCCACAGGAATCACCAGACCGGGAGGTCATGTGAAACCTGTGGGAGCGGGCGTGCCCGCGCACGGCCGCGAGGCGGCCCCATTTCACTTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTc                                                 >  7:144292/1‑146 (MQ=38)
                                                                                                           gCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTc                                          <  5:124196/149‑1 (MQ=255)
                                                                                                             aCGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGc                                        <  7:18369/149‑1 (MQ=255)
                                                                                                             aCGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGc                                        >  7:272448/1‑149 (MQ=255)
                                                                                                               gCCCGCGCCCCCAGGACTCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCg                                      <  5:337809/149‑1 (MQ=255)
                                                                                                                        cacaGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTAGATCTTCGCCGGCATCGCCt                             >  4:246446/1‑149 (MQ=21)
                                                                                                                         acaGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTc                            >  4:163486/1‑149 (MQ=255)
                                                                                                                         acaGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTc                            >  3:7090/1‑149 (MQ=255)
                                                                                                                          caGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTc                            >  8:45862/1‑148 (MQ=255)
                                                                                                                          caGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTc                            >  6:246501/1‑148 (MQ=255)
                                                                                                                            ggAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAg                         >  7:37004/1‑149 (MQ=255)
                                                                                                                             gAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAgc                        <  7:35472/149‑1 (MQ=255)
                                                                                                                                tCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCa                     <  6:172787/149‑1 (MQ=255)
                                                                                                                                  acatgACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCAt                    <  2:79358/144‑1 (MQ=37)
                                                                                                                                  acatgACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCAt                    >  1:79358/5‑148 (MQ=37)
                                                                                                                                   caaGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTAGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATa                   >  4:294453/3‑148 (MQ=255)
                                                                                                                                    atgACCTCCAGGTCTGGTGATTCCTGTTGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTc                            <  6:153410/136‑1 (MQ=37)
                                                                                                                                    atgACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCTGCAAAGCAGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATa                   >  6:354874/3‑147 (MQ=21)
                                                                                                                                    atgACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTc                            >  5:153410/3‑138 (MQ=37)
                                                                                                                                           ggAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGcc          >  7:140529/1‑149 (MQ=255)
                                                                                                                                           ccaGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGcc          >  8:21152/3‑149 (MQ=255)
                                                                                                                                              ggTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCAt       >  4:287719/1‑149 (MQ=255)
                                                                                                                                               gTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATc      <  6:177671/149‑1 (MQ=255)
                                                                                                                                                   tgtgATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATCACCt  >  6:181317/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATCACCT  >  NC_002947/1013102‑1013397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑