Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,025,788 C→A 7.8% A394S (GCG→TCG) ‡ dppA‑II ← dipeptide ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,025,7880CA 7.8% 178.1 / 4.3 60A394S (GCG→TCG) ‡dppA‑IIdipeptide ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (28/27);  new base A (3/2);  total (31/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.75e-01

GGTACCCAGCCAGTTGTCCGGGTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGCGCGTACGGGGCGTCCTTGAT  >  NC_002947/1025642‑1025935
                                                                                                                                                   |                                                                                                                                                   
ggTACCCAGCCAGTTGTCCGGGTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCt                                                                                                                                                   <  3:334528/149‑1 (MQ=255)
ggTACCCAGCCAGTTGTCCGGGTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCt                                                                                                                                                   >  3:137835/1‑149 (MQ=255)
ggTACCCAGCCAGTTGTCCGGGTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCt                                                                                                                                                   <  2:228297/149‑1 (MQ=255)
      cagccagTTGTCCGGGTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGCTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCa                                                                                                                                             >  8:85336/1‑149 (MQ=255)
       agccagTTGTCCGGGTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGGCG‑GACTGGAGCaa                                                                                                                                            <  7:148450/149‑2 (MQ=255)
           agTTGTCCGGGTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCg                                                                                                                                        >  6:96497/1‑149 (MQ=255)
           agTTGTCCGGGTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGGCG‑GACTGGAGCaagctc                                                                                                                                        <  4:229851/149‑6 (MQ=255)
                         ccGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCt                                                                                                                              <  8:265151/145‑1 (MQ=255)
                         ccGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCt                                                                                                                              >  7:265151/1‑145 (MQ=255)
                                 aCCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTgggg                                                                                                                   <  3:343049/148‑1 (MQ=255)
                                 aCCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTgggg                                                                                                                   <  3:42557/148‑1 (MQ=255)
                                   cGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTg                                                                                                                >  2:227923/1‑149 (MQ=255)
                                    ggTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGt                                                                                                               <  1:257048/149‑1 (MQ=255)
                                     gTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGTCCATCAGCTTGGCATTGGGGTTGTa                                                                                                              >  5:327751/1‑149 (MQ=255)
                                      tCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTAc                                                                                                             >  3:3334/1‑149 (MQ=255)
                                             gATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGtt                                                                                                      >  2:365293/1‑149 (MQ=255)
                                                 aGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCAc                                                                                                  >  5:135527/1‑149 (MQ=255)
                                                   ggCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTggg                                                                                                                    <  4:274479/129‑1 (MQ=255)
                                                   ggCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTggg                                                                                                                    >  3:274479/1‑129 (MQ=255)
                                                   ggCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCg                                                                                                >  7:196402/1‑149 (MQ=255)
                                                     cGATGTCGTGCGCACCGTTTTTCATGCGCTTGAGGTATGCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGCGCTTGCTCCAGTCC‑GCCTGGATCATTTCGGCCATCCGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGc                                                                                              <  3:321719/149‑1 (MQ=37)
                                                          tCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCGCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATgg                                                                                          <  5:275253/148‑1 (MQ=255)
                                                           cGTGCTCACCGGTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGcc                                                                                        <  4:265956/149‑1 (MQ=255)
                                                               cTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCcaca                                                                                    <  4:355588/149‑1 (MQ=255)
                                                                tCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAg                                                                                   >  4:201337/1‑149 (MQ=255)
                                                                tCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGAGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATAAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAg                                                                                   >  4:100369/1‑149 (MQ=255)
                                                                 cACCGGTTTTCATGCCCTTGAGGTATTCCCCCCATTCGTAGCTTAAGAAGCCCAACTTGAATCCGTGCTTGCTCCAGTGC‑CCCTTGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAgg                                                                                  <  7:286253/149‑1 (MQ=25)
                                                                   ccGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGt                                                                                <  5:96497/149‑1 (MQ=255)
                                                                        tttCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCACACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGAtct                                                                           >  1:78102/1‑149 (MQ=255)
                                                                          tCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGAtctc                                                                          <  1:227923/148‑1 (MQ=255)
                                                                            aTGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGt                                                                       <  2:202357/149‑1 (MQ=255)
                                                                                     aGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGttt                                                              >  6:323142/1‑149 (MQ=255)
                                                                                       gTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGa                                                            >  1:84617/1‑149 (MQ=255)
                                                                                          ttCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGacac                                                         <  8:196402/149‑1 (MQ=255)
                                                                                           tCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACAcc                                                        >  3:63225/1‑149 (MQ=255)
                                                                                            cGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACAcc                                                        >  1:97407/1‑149 (MQ=255)
                                                                                              ccccATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGc                                                     <  4:137835/149‑1 (MQ=255)
                                                                                               cccATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGcc                                                    >  8:252536/1‑149 (MQ=255)
                                                                                                  aTTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCAAGGTTTGACACCCGCctgc                                                 >  7:328664/1‑149 (MQ=255)
                                                                                                     cGTAGCAGACTCTGCGCACCTTGAAGCCTCTCTTGCTCCACGCC‑GCCAGCATCATTTCGGCCATCAGCTTGGCATTGGGGTTCTACGGGCGTTGCACCGGCATGGCCCACAGGGTTATCTCGTTGCCAGGTTTGACACCCTCctcctg                                               <  8:328664/148‑1 (MQ=255)
                                                                                                        aGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGAt                                            >  1:71283/1‑149 (MQ=255)
                                                                                                                aTGCGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCt                                   <  2:84617/149‑1 (MQ=255)
                                                                                                                    gcACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCctgct                                                >  1:326468/1‑132 (MQ=255)
                                                                                                                    gcACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCctgct                                                <  2:326468/132‑1 (MQ=255)
                                                                                                                     cACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCt                              <  4:3334/149‑1 (MQ=255)
                                                                                                                         ttGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTc                          >  4:32750/1‑149 (MQ=255)
                                                                                                                           gAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGTGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCGAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTcc                         <  6:327751/148‑1 (MQ=255)
                                                                                                                                 cgaaatGCTCCAGGCG‑GACTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGCCACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGcgcg                   >  3:359782/6‑148 (MQ=21)
                                                                                                                                 cgaaatGCTCCAGGCG‑GACTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGcgcg                   >  3:354182/6‑148 (MQ=255)
                                                                                                                                   aaatGCTCCAGGCG‑GACTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGCGCGTAc                >  6:218778/4‑149 (MQ=255)
                                                                                                                                     ttGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTc                          >  7:16340/1‑137 (MQ=255)
                                                                                                                                     ttGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTc                          <  8:16340/137‑1 (MQ=255)
                                                                                                                                      tGCTCCCGTCC‑CCCTCGAGCATTGCGGCCCTCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGCGCGTACggg             <  2:221441/149‑1 (MQ=37)
                                                                                                                                        cTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGCGCGTACGGGGc           >  1:194702/1‑149 (MQ=255)
                                                                                                                                               cc‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGTCCGTTTCCGGAGCGTACGGGGCGTCCtt     >  2:295432/1‑148 (MQ=255)
                                                                                                                                               cc‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGCGCGTACGGGGCGTCCtt     >  1:144496/1‑148 (MQ=255)
                                                                                                                                                c‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGCGCGTACGGGGCGTCCTTGa   <  5:323142/149‑1 (MQ=255)
                                                                                                                                                  gCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGCGCGTACgg              >  3:190582/1‑137 (MQ=255)
                                                                                                                                                  gCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGCGCGTACgg              <  4:190582/137‑1 (MQ=255)
                                                                                                                                                  gCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCCTTTCCGGCGCGTACGGGGCGTCCTTGAt  <  1:365293/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GGTACCCAGCCAGTTGTCCGGGTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCC‑GCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGCGCGTACGGGGCGTCCTTGAT  >  NC_002947/1025642‑1025935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑