Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,114,340 C→T 5.8% G156D (GGC→GAC)  rlmF ← ribosomal RNA large subunit methyltransferase F

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,114,3400CT 5.8% 204.9 / 2.4 70G156D (GGC→GAC) rlmFribosomal RNA large subunit methyltransferase F
Reads supporting (aligned to +/- strand):  ref base C (33/32);  new base T (2/2);  total (35/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-01

CGCTGGCTGCCGCGGGTGGCTTCGTCGCGTGAGGCATGGAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTGGATAGATGCAGTTGGCCCCCACGCCGATGTCCA  >  NC_002947/1114199‑1114475
                                                                                                                                              |                                                                                                                                        
cgcTGGCTGCCGCGGGTGGCTTCGTCGCGTGAGGCATGGAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGc                                                                                                                                    <  6:191332/148‑1 (MQ=255)
  ctggctgCCGCGGGTGGCTTCGTCGCGTGAGGCATGGAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTATCGTCCAGGcc                                                                                                                                   >  5:32099/1‑148 (MQ=255)
   tggctgCCGCGGGTGGCTTCGTCGCGTGAGGCATGGAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGc                                                                                                                                          >  8:276434/1‑139 (MQ=255)
   tggctgCCGCGGGTGGCTTCGTCGCGTGAGGCATGGAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGc                                                                                                                                          <  7:276434/139‑1 (MQ=255)
   tggctgCCGCGGGTGGCTTCGTCGCGTGAGGCATGGAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCAtt                                                                                                                                <  2:352509/149‑1 (MQ=255)
     gctgCCGCGGGTGGCTTCGTCGCGTGAGGCATGGAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCAtt                                                                                                                                <  2:255022/147‑1 (MQ=255)
     gctgCCGCGGGTGGCTTCGTCGCGTGAGGCATGGAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTgg                                                                                                                              >  7:257827/1‑149 (MQ=255)
      ctgCCGCGGGTGGCTTCGTCGCGTGAGGCATGGAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGc                                                                                                                             <  1:207696/149‑1 (MQ=255)
       tgCCGCGGGTGGCTTCGTCGCGTGAGGCATGGAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGcc                                                                                                                            >  8:255721/1‑149 (MQ=255)
           gcgGGTGGCTTCGTCGCGTGAGGCATGGAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑a                                                                                                                       <  5:276406/149‑1 (MQ=255)
           gcgGGTGGCTTCGTCGCGTGAGGCATGGAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑a                                                                                                                       <  6:288203/149‑1 (MQ=255)
                tGGCTTCGTCGCGTGAGGCATGGAAGGGCTGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATa                                                                                                                  <  4:75062/149‑1 (MQ=255)
                  gCTTCGTCGCGTGAGGCATGGAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCTATCGTCCAGGCCAATGGCCTgggcaaagc                                                                                                                  <  6:32099/149‑8 (MQ=255)
                    ttCGTCGCGTGAGGCATGGAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCt                                                                                                              >  1:37381/1‑149 (MQ=255)
                      cgtcgCGTGAGGCATGGAAGGGCGGGTTGCTCAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCTGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCtt                                                                                                             <  7:127480/148‑1 (MQ=255)
                        tcgCGTGAGGCATGGAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGc                                                                                                          <  3:198842/149‑1 (MQ=255)
                               aGGCATGGAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGTCCAGGCCAATGGCCTgggcaaag                                                                                                                   <  8:198831/134‑7 (MQ=255)
                               aGGCATGGAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGTCCAGGCCAATGGCCTgggcaaag                                                                                                                   >  7:198831/1‑128 (MQ=255)
                               aGGCATGGAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGc                                                                                                    >  6:177270/1‑148 (MQ=255)
                                 gCATGGAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGCTAGCCTTGGCCGAGGCCAg                                                                                                 >  7:187374/1‑149 (MQ=255)
                                   aTGGAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAgcg                                                                                               >  5:227391/1‑149 (MQ=255)
                                   aTGGAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAgcg                                                                                               >  8:317728/1‑149 (MQ=255)
                                     ggAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGcc                                                                                             >  3:200033/1‑149 (MQ=255)
                                           gCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCggg                                                                                       >  4:349401/1‑149 (MQ=255)
                                            cGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGt                                                                                      <  6:227391/149‑1 (MQ=255)
                                                ttGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGAt                                                                                  <  3:315051/149‑1 (MQ=255)
                                                ttGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGAt                                                                                  >  6:129662/1‑149 (MQ=255)
                                                    acaGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCg                                                                                         <  7:331990/138‑1 (MQ=255)
                                                    acaGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCg                                                                                         >  8:331990/1‑138 (MQ=255)
                                                    acaGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTcc                                                                              >  8:159858/1‑149 (MQ=255)
                                                    acaGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTcc                                                                              >  4:153891/1‑149 (MQ=255)
                                                    acaGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTcc                                                                              >  3:57964/1‑149 (MQ=255)
                                                      aGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTcc                                                                              >  7:4164/1‑147 (MQ=255)
                                                      aGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑ATGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTccgc                                                                            >  3:203993/1‑148 (MQ=255)
                                                         gtcaggtcaAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTccgagcc                                                                         >  5:18677/1‑149 (MQ=255)
                                                          tcaggtcaAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGC‑‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTccgagccga                                                                       <  4:159226/149‑1 (MQ=255)
                                                            aggtcaAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTccgagccga                                                                       <  8:257827/148‑1 (MQ=255)
                                                               tcaAAGCGTTCGCCCTCCTGCTGCATGCGGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGGT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGaa                                                                   <  2:286616/149‑1 (MQ=255)
                                                                caAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAg                                                                  >  6:258578/1‑149 (MQ=255)
                                                                 aaaGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTccgagccga                                                                       <  1:145869/143‑1 (MQ=255)
                                                                 aaaGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTccgagccga                                                                       >  2:145869/1‑143 (MQ=255)
                                                                 aaaGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGaa                                                                   >  1:286616/1‑147 (MQ=255)
                                                                 aaaGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAgc                                                                 <  6:129749/149‑1 (MQ=255)
                                                                      gTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAg                                                            <  7:317728/149‑1 (MQ=255)
                                                                      gTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAg                                                            <  7:255721/149‑1 (MQ=255)
                                                                            cctcctGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAg                                                      >  2:361165/1‑149 (MQ=255)
                                                                                    agcagGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTg                                                >  7:139131/1‑147 (MQ=255)
                                                                                    agcagGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTg                                                >  6:259960/1‑147 (MQ=255)
                                                                                       agGCCGCTGTGGATGTGCGCGGGGTTGGCCGGCGGGCGCTGGGTAATGGCT‑TTGCCCAGGCCATTGGCGTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGGCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGcc                                           <  6:18677/149‑1 (MQ=255)
                                                                                           cGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCGGAGCCGAGGAAGCGCCTGCGGTAGTCACTGTAGCCcag                                        >  6:313429/1‑148 (MQ=255)
                                                                                           cGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCcag                                        >  1:180949/1‑148 (MQ=255)
                                                                                           cGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCcag                                        >  7:228073/1‑148 (MQ=255)
                                                                                            gCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGaa                                                                   >  1:35041/1‑120 (MQ=255)
                                                                                            gCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGaa                                                                   <  2:35041/120‑1 (MQ=255)
                                                                                            gCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCcagca                                      <  1:361165/149‑1 (MQ=255)
                                                                                                aGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGt                                    <  7:159858/147‑1 (MQ=255)
                                                                                                  gATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTGGAt                                <  5:177270/149‑1 (MQ=255)
                                                                                                      tgCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTGGATAGAt                            <  3:153891/149‑1 (MQ=255)
                                                                                                       gcgcTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTGGATAGAt                            <  5:258578/148‑1 (MQ=255)
                                                                                                          cTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTGGATAGATGCAg                        <  3:349401/149‑1 (MQ=255)
                                                                                                             ggTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTGGATAGATGCAGTTg                     <  5:259960/149‑1 (MQ=255)
                                                                                                               ttGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTGGATAGATGCAGTTGGc                   <  7:147710/149‑1 (MQ=255)
                                                                                                               ttGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTGGATAGATGCAGTTGGc                   <  5:129662/149‑1 (MQ=255)
                                                                                                                  gCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGGAACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCGGCAGTGGATAGATGCAGTTGGccc                 >  1:269679/1‑149 (MQ=255)
                                                                                                                  gCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTGGATAGATGCAGTTGGcccc                <  8:228073/149‑1 (MQ=255)
                                                                                                                      gctgGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTGGATAGATGCAGTTGGCCCCCACg            >  4:77291/1‑149 (MQ=255)
                                                                                                                       ctgGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTGGATAGATGCAGTTGGCCCCCACGc           >  7:43085/1‑149 (MQ=255)
                                                                                                                           cgcAGGGTAATGGCT‑TTGGCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTGGATAGATGCAGTTGGCCCCCACGCCGAt       <  2:37381/149‑1 (MQ=255)
                                                                                                                            gcAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTGGATAGATGCAGTTGGCCCCCACGCCGATg      >  3:114827/1‑149 (MQ=255)
                                                                                                                              aGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTGGATAGATGCAGTTGGCCCCCACGCCGATGTc    >  5:190935/1‑149 (MQ=255)
                                                                                                                                ggTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGCGGGCAGCGCCAGCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCGGTGGATAGATGCAGTGGGCCCCCACGCCGATGTc    >  7:254403/1‑147 (MQ=255)
                                                                                                                                ggTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTGGATAGATGCAGTTGGCCCCCACGCCGATGTCCa  <  3:77291/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                        
CGCTGGCTGCCGCGGGTGGCTTCGTCGCGTGAGGCATGGAAGGGCGGGTTGCACAGGGTCAGGTCAAAGCGTTCGCCCTCCTGCAGCAGGCCGCTGAGGATGTGCGCTCGGTTGGCCTGCTGGCGCAGGGTAATGGCT‑TTGCCCAGGCCATTGGCCTGG‑ACGATAGCCTTGGCCGAGGCCAGCGCCACCGGGTCGATGTCCGAGCCGAGGAAGCGCCAGCGGTAGTCACTGTAGCCCAGCAGTGGATAGATGCAGTTGGCCCCCACGCCGATGTCCA  >  NC_002947/1114199‑1114475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑