Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,175,201 A→G 8.2% E92E (GAA→GAG)  PP_1030 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,175,2010AG 8.2% 174.8 / 4.6 61E92E (GAA→GAG) PP_1030hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (28/28);  new base G (2/3);  total (30/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAACCGCATGGGCCATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTC‑GGCGAACCGGTCAACGGCCTGGAAGTGATGCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACATCAATGGCTTCATTTACCTGCAGCCA  >  NC_002947/1175059‑1175348
                                                                                                                                               |                                                                                                                                                   
gTAACCGCATGGGCCATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTC‑GGCGAACCGGTCAACGGCCTGGAAGTGATGCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGcc                                                                                                                                               >  3:44714/1‑149 (MQ=255)
 tAACCGCATGGGCCATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTC‑GGCGAACCGGTCAACGGCCTGGAAGTGATGCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCTTCGAGGAAGcct                                                                                                                                              <  5:78966/149‑2 (MQ=255)
   aCCGCATGGGCCATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTC‑GGCGAACCGGTCAACGGCCTGGAAGTGATGCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCTTCGAGGAAGccttc                                                                                                                                            <  6:209129/149‑4 (MQ=255)
    ccGCATGGGCCATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTC‑GGCGAACCGGTCAACGGCCTGGAAGTGATGCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCg                                                                                                                                              >  5:67335/1‑146 (MQ=255)
    ccGCATGGGCCATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTC‑GGCGAACCGGTCAACGGCCTGGAAGTGATGCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCg                                                                                                                                              <  6:67335/146‑1 (MQ=255)
     cGCATGGGCCATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTC‑GGCGAACCGGTCAACGGCCTGGAAGTGATGCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTg                                                                                                                                          >  1:160329/1‑149 (MQ=255)
        aTGGGCCATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTC‑GGCGAACCGGTCAACGGCCTGGAAGTGATGCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGccc                                                                                                                                       >  6:370035/1‑149 (MQ=255)
                       gcggcAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTC‑GGCGACCCGGTCAACGGCCTGGAAGTGATGCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGa                                                                                                                        <  5:382401/149‑1 (MQ=255)
                       gcggcAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTC‑GGCGAACCGGTCAACGGCCTGGAAGTGATGCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGa                                                                                                                        <  7:258126/149‑1 (MQ=255)
                       gcggcAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTC‑GGCGAACCGGTCAACGGCCTGGAAGTGATGCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGa                                                                                                                        <  6:13586/149‑1 (MQ=255)
                         ggcAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTC‑GGCGAACCGGTCAACGGCCTGGAAGTGATGCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGagg                                                                                                                      <  2:279920/149‑1 (MQ=255)
                            aGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTC‑GGCGAACCGGTCAACGGCCTGGAAGTGATGCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGaggtag                                                                                                                   <  5:9872/149‑1 (MQ=255)
                            aGGGGCGCGCAAAGTGGCCCTGCACACGGAAAAACTGCCCTTC‑GGCGAACCGGTCAACGGCCTGGAAGTGATGCTCAAACGCTGCATCTATACACCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGaggtag                                                                                                                   >  2:45826/1‑149 (MQ=255)
                                gcgcgcAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTC‑GGCGAACCGGTCAACGGCCTGGAAGTGATGCTCAACCGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGa                                                                                                               <  5:370035/149‑1 (MQ=255)
                                  gcgcAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTC‑GGCGAACCGGTCAACGGCCTGGAAGTGATGCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGc                                                                                                             >  5:347653/1‑149 (MQ=255)
                                      aaaGTGGCCCTGCACCCGGAAAAACTGCCCTTC‑GGCGAACCGGTCAACGGCCTGGAAGTGATGCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCgtt                                                                                                         >  7:220243/1‑149 (MQ=255)
                                             cccTGCACCCGGAAAAACTGCCCTTC‑GGCGAACCGGGCAACGGCCTGGAAGTGATGCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGaa                                                                                                  <  4:19199/149‑1 (MQ=255)
                                             cccTGCACCAGGAAAAACTGCCCTTC‑GGCGAACCGGTCAACGGCCTGGAAGTGATGCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGaa                                                                                                  <  4:14128/149‑1 (MQ=255)
                                              ccTGCACCCGGAAAAACTGCCCTTCGGGCGAACCGGTCAACGGCCTGGAAGTGATGCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGCTCCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCg                                                                                                    >  1:201053/1‑147 (MQ=255)
                                              ccTGCACCCGGAAAAACTGCCCTTC‑GGCGAACCGGTCAACGGCCTGGAAGTGATGCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGCTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCg                                                                                                    <  2:201053/146‑1 (MQ=255)
                                                         aaaaaCTGCCCTTC‑GGCGAACCGGTCAACGGCCTGGAAGTGATGCTCAAACGCTGCATCTATACCCACACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAagag                                                                                      >  1:297765/1‑149 (MQ=255)
                                                          aaaaCTGCCCTTC‑GGCGAACCGGTCAACGGCCTGGAAGTGATGCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCTGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGt                                                                                     <  1:275252/149‑1 (MQ=255)
                                                           aaaCTGCCCTTC‑GGCGAACCGGTCAACGGCCTGGAAGTGATGCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTg                                                                                    <  2:314696/149‑1 (MQ=255)
                                                                         gCGAACCGGTCAACGGCCTGGAAGTGATGCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTg                                                                       <  2:160329/149‑1 (MQ=255)
                                                                          cGAACCGGTCAACGGCCTGGAAGTGATGCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGTAGt                                                                        >  7:145945/1‑147 (MQ=255)
                                                                           gAACCGGTCAACGGCCTGGAAGTGATGCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAg                                                                     >  5:147885/1‑149 (MQ=255)
                                                                                ggTCAACGGCCTGGAAGTGATGCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGAc                                                                 >  5:122046/1‑148 (MQ=255)
                                                                                 gTCAACGGCCTGGACGTGATGATCAACCGCTGCATCTATCCCCCCACCGCAGGCTTCCTCGAAGCAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAACCCTGGAAGAGTGGATGCCGGCAGTGAGCGACaa                                                               <  6:303170/149‑1 (MQ=255)
                                                                                 gTCAACGGCCTGGAAGTGATGCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTg                                                                                            >  3:303389/1‑120 (MQ=255)
                                                                                 gTCAACGGCCTGGAAGTGATGCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTg                                                                                            <  4:303389/120‑1 (MQ=255)
                                                                                 gTCAACGGCCTGGAAGTGATGCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGGTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGAcga                                                               >  4:342053/1‑147 (MQ=255)
                                                                                  tCAACGGCCTGGAAGTGATGCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAAc                                                              <  6:347653/149‑1 (MQ=255)
                                                                                   cAACGGCCTGGAAGTGATGCTCAAACGCTGCATCTAAACCCCCACCGAAGGCTTCCTCGAAGAAGCCAGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACt                                                             >  1:248463/1‑149 (MQ=255)
                                                                                    aaCGGCCTGGAAGTGATGCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACtt                                                            >  6:62294/1‑149 (MQ=255)
                                                                                      cGGCCTGGAAGTGATGCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTa                                                          >  2:341971/1‑149 (MQ=255)
                                                                                        gCCTGTAAGTGATGCTCAAACGCTGCATCTATACCCACACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGAATGCCGGCAGTGAGCGACAACTTTAcc                                                        <  4:100797/149‑1 (MQ=255)
                                                                                           tGGAAGTGATGCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGc                                                     >  5:202384/1‑149 (MQ=255)
                                                                                                      cTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCgg                                          >  8:178/1‑149 (MQ=255)
                                                                                                      cTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCgg                                          >  3:307896/1‑149 (MQ=255)
                                                                                                          aaCGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTc                                      <  7:178/149‑1 (MQ=255)
                                                                                                             gCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGaa                                   <  2:297765/149‑1 (MQ=255)
                                                                                                              ctacATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCg                                     >  3:104177/4‑146 (MQ=255)
                                                                                                                 cATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACCACTTTACCTGCCCACTGTGCGGCTTCGAAGAt                                <  1:45826/148‑1 (MQ=255)
                                                                                                                  ctccataCCCCCACCGCAGGCTTCCTCGCAGACGCCGGTTGCCCGCAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGa                              <  4:223250/145‑1 (MQ=255)
                                                                                                                      ataCCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGc                                            >  4:301355/1‑131 (MQ=255)
                                                                                                                      ataCCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGc                                            <  3:301355/131‑1 (MQ=255)
                                                                                                                             cACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACATCAATGGCt                   >  4:300475/1‑149 (MQ=255)
                                                                                                                               ccGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACATCAATGGCTTc                 <  4:338899/149‑1 (MQ=255)
                                                                                                                                 gAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACATCAATGGCTTCAt               <  3:300475/149‑1 (MQ=255)
                                                                                                                                 gAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACATCAATGGCTTCAt               >  6:337483/1‑149 (MQ=255)
                                                                                                                                 accggCTTCTTCGAGGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTAc                                                         <  3:115662/104‑1 (MQ=255)
                                                                                                                                 accggCTTCTTCGAGGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTAc                                                         >  4:115662/4‑107 (MQ=255)
                                                                                                                                 accggCTTCTTCGAGGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACATCAATGGCTTCAt               >  3:47656/4‑149 (MQ=255)
                                                                                                                                     gCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACATCAATGGCTTCATTTAc           <  6:147885/149‑1 (MQ=255)
                                                                                                                                     gCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACATCAATGGCTTCATTTAc           <  4:44714/149‑1 (MQ=255)
                                                                                                                                     gCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACATCAATGGCTTCATTTAc           <  4:104177/149‑1 (MQ=255)
                                                                                                                                      cTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACATCAATGGCTTCATTTAcc          >  8:164515/1‑149 (MQ=255)
                                                                                                                                       ttCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTAc                                                         <  2:229190/101‑1 (MQ=255)
                                                                                                                                       ttCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGACGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTAc                                                         >  1:229190/1‑101 (MQ=255)
                                                                                                                                         ccTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACATCAATGGCTTCATTTACCTg        >  5:360326/1‑148 (MQ=255)
                                                                                                                                          cTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACATCAATGGCTTCATTTACCTg        >  2:314802/1‑147 (MQ=255)
                                                                                                                                             gaagtaTCCGGTTGCCGGGAGTGCGGGCGCGAGGTAGGTGAGCGGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACATCATTGGCTTCATTTAACTGCat     <  2:248463/147‑2 (MQ=255)
                                                                                                                                             gaagaaGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACATCAATGGCTTCATTTACCTGCAGc    >  4:179015/1‑148 (MQ=255)
                                                                                                                                              aagaagCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACATCAATGGCTTCATTTACCTGCAGCCa  >  2:69122/1‑149 (MQ=255)
                                                                                                                                              aagaagCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACATCAATGGCTTCATTTACCTGCAGCCa  >  6:47328/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
GTAACCGCATGGGCCATGCCCTGGCGGCAGGGGCGCGCAAAGTGGCCCTGCACCCGGAAAAACTGCCCTTC‑GGCGAACCGGTCAACGGCCTGGAAGTGATGCTCAAACGCTGCATCTATACCCCCACCGAAGGCTTCCTCGAAGAAGCCGGTTGCCCGGAGTGCCGGCGCGAGGTAGGTGAGCCGTTGTTCGAAAGCCTGGAAGAGTGGATGCCGGCAGTGAGCGACAACTTTACCTGCCCACTGTGCGGCTTCGAAGATGACATCAATGGCTTCATTTACCTGCAGCCA  >  NC_002947/1175059‑1175348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑