Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,212,521	C→T	100%	intergenic (‑55/+133)	PP_1060 ← / ← PP_1061	glutamate synthase large subunit/ATP‑dependent DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,212,521	0	C	T	100.0%	231.5 / NA	68	intergenic (‑55/+133)	PP_1060/PP_1061	glutamate synthase large subunit/ATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/31);  total (37/31)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACCATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGCCGTGCACCGTTGCCGCCGCTACCGTATTC  >  NC_002947/1212376‑1212664                                                                                                                                                   |                                                                                                                                                aCCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACtata                                                                                                                                              <  4:354406/149‑1 (MQ=255)     ttCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGcc                                                                                                                                           >  2:75583/1‑148 (MQ=255)       cGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCaa                                                                                                                                         <  7:36344/148‑1 (MQ=255)       cGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCaa                                                                                                                                         <  1:368694/148‑1 (MQ=255)         tggtggCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGtt                                                                                                                                      >  7:69491/1‑149 (MQ=255)                tGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCACACCCCGACTATAGCCAAGTTGCCggg                                                                                                                                >  8:130906/1‑148 (MQ=255)                     gggAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTg                                                                                                                           >  4:33047/1‑148 (MQ=255)                         ggCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCaaaa                                                                                                                      >  1:367964/1‑149 (MQ=255)                              gCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCGCTGCaaaataaa                                                                                                                  <  5:8198/148‑1 (MQ=255)                               ccAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCaaaataaaat                                                                                                                >  1:62013/1‑149 (MQ=255)                                  gggTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCgg                                                                                                             <  5:50647/149‑1 (MQ=255)                                   ggTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCggg                                                                                                            >  1:101074/1‑149 (MQ=255)                                     tGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCgggg                                                                                                           >  5:51957/1‑148 (MQ=255)                                               acaGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATGAGCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGaaaa                                                                                                  >  1:293981/1‑148 (MQ=255)                                               acaGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAAt                                                                                                 >  8:138000/1‑148 (MQ=255)                                                caGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCa                                                                                               <  5:279313/149‑1 (MQ=255)                                                 aGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCAt                                                                                              <  4:325785/149‑1 (MQ=255)                                                   gcggcgAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATtc                                                                                            >  6:364924/1‑149 (MQ=255)                                                        gaagaaGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAg                                                                                       >  8:230678/1‑149 (MQ=255)                                                            aagGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTa                                                                                   <  8:69491/149‑1 (MQ=255)                                                            aagGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTa                                                                                   <  1:75583/149‑1 (MQ=255)                                                                 aTAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAACTCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGc                                                                              >  4:338274/1‑149 (MQ=255)                                                                 aTAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGc                                                                              >  2:364644/1‑149 (MQ=255)                                                                  tAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGc                                                                                                                               <  2:224868/99‑1 (MQ=255)                                                                  tAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGc                                                                                                                               >  1:224868/1‑99 (MQ=255)                                                                   aaCGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCgg                                                                            >  1:50061/1‑149 (MQ=255)                                                                   aaCGACTGAGAAGTGACAGGCTCATACAGGGTCCCTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCgg                                                                            >  2:349096/1‑149 (MQ=255)                                                                     cGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGt                                                                          <  3:33047/149‑1 (MQ=255)                                                                        cTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCa                                                                       >  3:69077/1‑149 (MQ=255)                                                                           agaAGTGACAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCAcc                                                                     <  2:293981/148‑1 (MQ=255)                                                                             aaGTGACAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCcgc                                                                  <  2:236254/149‑1 (MQ=255)                                                                              aGTGACAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCcgcg                                                                 <  5:364924/149‑1 (MQ=255)                                                                                 gACAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTACAAAATAAAATCGGGGTAGc                                                                                                       <  1:1113/108‑1 (MQ=255)                                                                                 gACAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTACAAAATAAAATCGGGGTAGc                                                                                                       >  2:1113/1‑108 (MQ=255)                                                                                  aCAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGaa                                                               <  7:130906/147‑1 (MQ=255)                                                                                    aGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAg                                                             >  7:323896/1‑147 (MQ=255)                                                                                    aGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAg                                                             <  8:323896/147‑1 (MQ=255)                                                                                        tCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAgggccgg                                                       >  7:271122/1‑149 (MQ=255)                                                                                        tCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAgggccgg                                                       >  8:332551/1‑149 (MQ=255)                                                                                         cATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAgggccggg                                                      >  2:185295/1‑149 (MQ=255)                                                                                          ttACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAgggccgggc                                                     <  5:368764/148‑1 (MQ=255)                                                                                          aTACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCgg                                                                            <  4:53295/126‑1 (MQ=255)                                                                                          aTACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCgg                                                                            >  3:53295/1‑126 (MQ=255)                                                                                          aTACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAgggccgg                                                       <  2:101074/147‑1 (MQ=255)                                                                                            aCAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAgggccgggc                                                     >  6:368764/1‑147 (MQ=255)                                                                                              aGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCctgc                                                 <  2:62013/149‑1 (MQ=255)                                                                                                ggTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCgg                                                                                                                                 <  7:198270/67‑1 (MQ=255)                                                                                                ggTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCgg                                                                                                                                 >  8:198270/1‑67 (MQ=255)                                                                                                   ccTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTACCGAAAATCATTCTCGAGCGTAGGATCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGc                                             >  6:258177/1‑148 (MQ=255)                                                                                                       gCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAAtc                                        >  7:9946/1‑149 (MQ=255)                                                                                                                gcgGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACccg                                <  2:367964/149‑1 (MQ=255)                                                                                                                    tCAGGCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGCCGt                            <  4:69077/149‑1 (MQ=255)                                                                                                                        gCTCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGCCGTGCAc                        >  5:316073/1‑149 (MQ=255)                                                                                                                         cTCAGGGCCTGGGGGCAACCCCCGACTATAGCCAAGTTGCCGCGCACTGCAAAATAACATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGACCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGCCGTGCAcc                       <  6:384946/149‑1 (MQ=37)                                                                                                                          tCAGGGCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGCCGTGCACCg                      >  4:229059/1‑149 (MQ=255)                                                                                                                              ggCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGCCGTGCACCGTTgc                  >  2:97741/1‑149 (MQ=255)                                                                                                                              ggCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGCCGTGCACCGTTgc                  <  8:271122/149‑1 (MQ=255)                                                                                                                              ggCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGCCGTGCACCGTTgc                  <  6:51957/149‑1 (MQ=255)                                                                                                                               gCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGCCGTGCACCGTTgcc                 <  7:138000/149‑1 (MQ=255)                                                                                                                               gCCTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGCCGTGCACCGTTgcc                 >  7:39528/1‑149 (MQ=255)                                                                                                                                 cTGGGGGCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGCCGTGCACCGTTgccgc               >  4:171549/1‑149 (MQ=255)                                                                                                                                    ggggCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGCCGTGCACCGTTGccgccg             <  6:316073/148‑1 (MQ=255)                                                                                                                                      ggCAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGCCGTGCACCGTTGCCGCCGCTa          <  1:185295/149‑1 (MQ=255)                                                                                                                                        cAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGCCGTGCACCGTTGCCGCCGCTAcc        <  1:349096/149‑1 (MQ=255)                                                                                                                                        cAAACCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGCCGTGCACCGTTGCCGCCGCTAcc        <  2:50061/149‑1 (MQ=255)                                                                                                                                         aaaCCCCGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGCCGTGCACCGTTGCCGCCGCTACCg       >  3:60125/1‑149 (MQ=255)                                                                                                                                             cccGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGCCGTGCACCGTTGCCGCCGCTGACGTAtt   >  4:141798/1‑149 (MQ=255)                                                                                                                                             cccGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGCCGTGCACCGTTGCCGCCGCTACCGTAtt   >  4:320844/1‑149 (MQ=255)                                                                                                                                              ccGACTATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGCCGTGCACCGTTGCCGCCGCTACCGTATTc  >  4:137194/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                ACCATTCGTGGTGGCTGAAGGGGAGGCTCGCCAGGGTGAACAGCACACAGGCGGCGAAGAAGGCATAACGACTGAGAAGTGACAGGCTCATACAGGGTCCTTGCGATG‑GCGCGGTCAGGCTCAGGGCCTGGGGGCAAACCCCGACCATAGCCAAGTTGCCGGGCACTGCAAAATAAAATCGGGGTAGCGAAAATCATTCTCGAGCGTAGGAGCGGGTTCACCCGCGAAAGGGCCGGGCCTGCTGGCCAATCTCCACCCGCCGTGCACCGTTGCCGCCGCTACCGTATTC  >  NC_002947/1212376‑1212664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑