Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,219,704 G→A 6.5% R84C (CGC→TGC)  glmP ← alginate biosynthesis membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,219,7040GA 6.5% 182.4 / 2.3 61R84C (CGC→TGC) glmPalginate biosynthesis membrane protein
Reads supporting (aligned to +/- strand):  ref base G (29/28);  new base A (2/2);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCATCCGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATACGGAATGATCGACCACATGCCAAACAGGATGGTGGTCTTGAGGTCGGCAATGCTGCGCCCCTTGCCGACGATGTAGTGGGCAATCAGGGCGAAGGTGGG  >  NC_002947/1219559‑1219848
                                                                                                                                                 |                                                                                                                                                
cAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCATCCGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGgcgc                                                                                                                                                 >  3:207837/1‑147 (MQ=255)
cAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCATCCGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCaa                                                                                                                                               <  2:304750/149‑1 (MQ=255)
 aGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCATCCGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAg                                                                                                                                              >  4:165032/1‑149 (MQ=255)
 aGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCATCCGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAg                                                                                                                                              <  1:248999/149‑1 (MQ=255)
 aGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCATCCGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAg                                                                                                                                              >  5:69662/1‑149 (MQ=255)
  ggCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCATCCGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGc                                                                                                                                             >  7:147442/1‑149 (MQ=255)
         aGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCATCCGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGAc                                                                                                                                      <  3:66264/149‑1 (MQ=255)
           gCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCATCCGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCa                                                                                                                                    <  2:343285/149‑1 (MQ=255)
              agagGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCATCCGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGta                                                                                                                                 <  3:165032/149‑1 (MQ=255)
                   tCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCATCCGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGtacata                                                                                                                             >  4:180250/1‑148 (MQ=255)
                     aCGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCATCCGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGtacataca                                                                                                                           <  4:207837/148‑1 (MQ=255)
                      cGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCATCCGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAgg                                                                                                                         <  7:344640/149‑1 (MQ=255)
                      cGCACATCGCAGGGCTCTGAATCGGCGGCGATTTCGCTCAGTGCATCCGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAgg                                                                                                                         <  6:171081/149‑1 (MQ=255)
                         acaTCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCATCCGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGtt                                                                                                                      <  8:52960/149‑1 (MQ=255)
                              gCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCATCCGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCa                                                                                                                  <  4:22733/148‑1 (MQ=255)
                              gCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCATCCGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCa                                                                                                                  <  8:156713/148‑1 (MQ=255)
                              gCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCATCCGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAg                                                                                                                 <  4:272467/149‑1 (MQ=255)
                                   gCTCTGAATCGGCGTCGATTTCGCTCAGTGCATCCGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTaca                                                                                                            >  3:189619/1‑149 (MQ=255)
                                     tctGAATCGGCGTCGATTTCGCTCAGTGCATCCGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTacac                                                                                                           >  2:105841/1‑148 (MQ=255)
                                         aaTCGGCGTCGATTTCGCTCAGTGCATCCGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGaaa                                                                                                       <  2:299274/148‑1 (MQ=255)
                                              gagTCGATTTCGCTCAGTGAATCCGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCACAAGCGGTCGACCAGTACATACAGGGTTGGCAGGTACACGAATTACgg                                                                                                  >  4:103906/3‑148 (MQ=255)
                                              gCGTCGATTTCGCTCAGTGCATCCGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCACAAGCGGTCGACCAGTACATACAGGGATGCCAGGTACACGAAATACgg                                                                                                  >  4:98673/1‑148 (MQ=255)
                                                    aTTTCGCTCAGTGCATCCGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATACGGAATGATc                                                                                           <  6:71468/149‑1 (MQ=255)
                                                     tttCGCTCAGTGCATCCGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATACGGAATGATCg                                                                                          <  8:147442/149‑1 (MQ=255)
                                                      ttCGCTCAGTGCATGCGCACCCACACAGTCACCAGCACTGTCGCGTCGATCAGCCAGGCCACTGTGTCCAGCGCCATCTAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATACg                                                                                                   <  8:337239/139‑1 (MQ=255)
                                                      ttCGCTCAGTGCATCCGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGACAGGCAACTGTGGCCAGCGCCATCGAAGCCTCGATGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTGCACGAAATACg                                                                                                   >  7:337239/1‑139 (MQ=255)
                                                            cAGTGCATCCGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATACGGAATGATCGACCACAt                                                                                   <  4:270458/149‑1 (MQ=255)
                                                             aGTGCATCCGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATACGGAATGATCGACcaca                                                                                    <  6:69662/147‑1 (MQ=255)
                                                                 cATCCGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATACGGAATGATCGACCACATGCCa                                                                               >  8:324927/1‑148 (MQ=255)
                                                                    ccGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAACAACCGAATGATCGACCCCCTGCCaaa                                                                             >  7:68847/1‑147 (MQ=255)
                                                                    ccGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATACGGAATGATCGACCACATGCCaaa                                                                             >  4:116934/1‑147 (MQ=255)
                                                                      gCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATACGGAATGATCg                                                                                          >  1:136233/1‑132 (MQ=255)
                                                                      gCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATACGGAATGATCg                                                                                          <  2:136233/132‑1 (MQ=255)
                                                                          ccACACAGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGAACACGAAATACGGAATGATCGACCACATGCCAAACAGGAtgg                                                                     >  1:164579/1‑149 (MQ=255)
                                                                            acacaGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATCCGGAATGATCGACCACAAGCCAAACAGGAtggtg                                                                   >  4:247826/1‑149 (MQ=255)
                                                                               caGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATACGGAATGATCGACCACATGCCAAACAGGATGGTGGTc                                                                >  3:325468/1‑149 (MQ=255)
                                                                               caGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATACGGAATGATCGACCACATGCCAAACAGGATGGTGGTc                                                                <  3:180250/149‑1 (MQ=255)
                                                                                 gTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATACGGAATGATCGACCACATGCCAAACAGGATGGTGGTCtt                                                              <  1:105841/149‑1 (MQ=255)
                                                                                    aCCAGCACGGTCGCGGCGATCCGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACAAACAGGGTTGCCAGGTACACGAAATACGGaa                                                                                                >  7:104776/1‑112 (MQ=255)
                                                                                    aCCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATACGGAATGATCGACCACATGCCAAACAGGATGGTGGTCTTGAg                                                           >  3:330199/1‑149 (MQ=255)
                                                                                        gCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATACGGAATGATCGACCACATGCCAAACAGGATGGTGGTCTTGAGGTCg                                                       >  8:106664/1‑149 (MQ=255)
                                                                                                 cggcgATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATACGGAATGATCGACCACATGCCAAACAGGATGGTGGTCTTGAGGTCGGCAAtgctg                                              >  2:309524/1‑149 (MQ=255)
                                                                                                     gATCAGCCAGGCCACTGTGGCCAGTGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATACGGAATGATCGACCACATGCCAAACAGGAtg                                                                      >  8:21012/1‑121 (MQ=255)
                                                                                                     gATCAGCCAGGCCACTGTGGCCAGTGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATACGGAATGATCGACCACATGCCAAACAGGAtg                                                                      <  7:21012/121‑1 (MQ=255)
                                                                                                     gATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATACGGAATGATCGACCACATGCCAAACAGGATGGTGGTCTTGAGGTCGGCAATGCTGCGcc                                          >  8:86656/1‑149 (MQ=255)
                                                                                                          gCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGGAATAGGGAATGATCGACCACAGGCCAAACCGGATGGTGGGCTTGCGGTCGGCAATGCTGCGCCCCTTg                                     >  8:332920/1‑149 (MQ=255)
                                                                                                          gCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATACGGAATGATCGACCACATGCCAAACAGGATGGTGGTCTTGAGGTCGGCAATGCTGCGCCCCTTg                                     >  2:248488/1‑149 (MQ=255)
                                                                                                          gCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATACGGAATGATCGACCACATGCCAAACAGGATGGTGGTCTTGAGGTCGGCAATGCTGCGCCCCTTg                                     >  8:46144/1‑149 (MQ=255)
                                                                                                           ccAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATACGGAATGATCGACCACATGCCAAACAGGATGGTGGTCTTGAGGTCGGCAATGCTGCGCCCCTTGc                                    >  2:354278/1‑149 (MQ=255)
                                                                                                             aGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATACGGAATGATCGACCACATGCCAAACAGGATGGTGGTCTTGAGGTCGGCAATGCTGCGCCCCTTGcc                                   >  8:12169/1‑148 (MQ=255)
                                                                                                                ccACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATACGGAATGATCGACCACATGCCAAACAGGATGGTGGTCTTGAGGGCGGCAATGCTGCGCCCCTTGCcgacg                               <  4:50018/149‑1 (MQ=255)
                                                                                                                 cACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATACGGAATGATCGACCACATGCCAAACAGGATGGTGGTCTTGAGGTCGGCAATGCTGCGCCCCTTGCcgacga                              >  6:314410/1‑149 (MQ=255)
                                                                                                                 cACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATACGGAATGATCGACCACATGCCAAACAGGATGGTGGTCTTGAGGTCGGCAATGCTGCGCCCCTTGCcgacga                              >  6:212492/1‑149 (MQ=255)
                                                                                                                                cATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATACGGAATGATCGACCACATGCCAAACAGGATGGTGGTCTTGAGGt                                                         <  2:283593/107‑1 (MQ=255)
                                                                                                                                cATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATACGGAATGATCGACCACATGCCAAACAGGATGGTGGTCTTGAGGt                                                         >  1:283593/1‑107 (MQ=255)
                                                                                                                                       gCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATACGGAATGATCGACCACATGCCAAACAGGATGGTGGTCTTGAGGTCGGCAATGCTGCGCCCCTTGCCGACGATGTAGTGGGCAATCAGGGCGaa        <  7:46144/149‑1 (MQ=255)
                                                                                                                                         cTCGAGGCACAAGCGGTCGACCAGTACATTCAGGGTTGCCAGGTACACGAAATACGGAATGATCGACCACATGCCAAACAGGATGGTGGTCTTGAGGTCGGCAATGCTGCGCCCCTTGCCGACGATGTAGTGGGCAATCAGGGCGAAgg      <  3:103906/149‑1 (MQ=255)
                                                                                                                                         cTCGAGGCACAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATACGGAATGATCGACCACATGCCAAACAGGATGGTGGTCTTGAGGTCGGCAATGCTGCGCCCCTTGCCGACGATGTAGTGGGCAATCAGGGCGAAgg      <  3:98673/149‑1 (MQ=255)
                                                                                                                                          tCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATACGGAATGATCGACCACATGCCAAACAGGATGGTGGTCTTGAGGTCGGCAATGCTGCGCCCCTTGCCGACGATGTAGTg                       >  1:16878/1‑131 (MQ=255)
                                                                                                                                          tCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATACGGAATGATCGACCACATGCCAAACAGGATGGTGGTCTTGAGGTCGGCAATGCTGCGCCCCTTGCCGACGATGTAGTg                       <  2:16878/131‑1 (MQ=255)
                                                                                                                                           cGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATACGGAATGATCGACCACATGCCAAACAGGATGGTGGTCTTGAGGTCGGCAATGCTGCGCCCCTTGCCGACGATGTAGTGGGCAATCAGGGCGAAGGTg    <  4:189619/149‑1 (MQ=255)
                                                                                                                                             aGGCGCAAGCGGTCGACCTGTACATACAGGGTTGCCAGGTACACGAAATACGGAATGATCGACCACATGCCAAACAGGAGGGTGGTCTTGAGGTCGGCAATGCTGCGCCCCTTGCCGACGATGTAGTGGGCAATCAGGGCGAAGGTggg  <  3:247826/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CAGGCGCACAGGCAAGAGGTCACGCACATCGCAGGGCTCTGAATCGGCGTCGATTTCGCTCAGTGCATCCGCACCCACACAGTCACCAGCACGGTCGCGGCGATCAGCCAGGCCACTGTGGCCAGCGCCATCGAAGCCTCGAGGCGCAAGCGGTCGACCAGTACATACAGGGTTGCCAGGTACACGAAATACGGAATGATCGACCACATGCCAAACAGGATGGTGGTCTTGAGGTCGGCAATGCTGCGCCCCTTGCCGACGATGTAGTGGGCAATCAGGGCGAAGGTGGG  >  NC_002947/1219559‑1219848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑