Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,221,037 T→G 6.1% D90A (GAC→GCC)  dctD‑II ← C4‑dicarboxylate transport transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,221,0370TG 6.1% 201.0 / 3.0 66D90A (GAC→GCC) dctD‑IIC4‑dicarboxylate transport transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (30/32);  new base G (2/2);  total (32/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01

GCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGCCGATGCGTTCCAGCGC  >  NC_002947/1220893‑1221185
                                                                                                                                                |                                                                                                                                                    
gCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGa                                                                                                                                                   >  7:158489/1‑148 (MQ=255)
  cAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGata                                                                                                                                                 >  6:76026/1‑148 (MQ=255)
         cggcgCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCAATATCGCCATg                                                                                                                                         <  4:358516/149‑1 (MQ=255)
              cAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAg                                                                                                                                    <  1:176221/149‑1 (MQ=255)
               aGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGt                                                                                                                                   <  5:280584/149‑1 (MQ=255)
               aGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGACAGt                                                                                                                                   >  4:337599/1‑149 (MQ=255)
                 ggCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGa                                                                                                                                 >  8:343978/1‑149 (MQ=255)
                 ggCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGGCGATATCGACATGGccgttgg                                                                                                                                 <  2:21076/149‑6 (MQ=255)
                    cgccacTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCa                                                                                                                              <  7:291485/147‑1 (MQ=255)
                    caccacTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCAAACGGCCATGTCGATATCTCCAAGGCCAGTGAt                                                                                                                                >  6:350019/1‑147 (MQ=255)
                       cacTTCGCGTGACAGCCCGCGCTGTACCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGca                                                                                                                           >  8:247264/1‑149 (MQ=255)
                              cgTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCgg                                                                                                                    <  5:109228/149‑1 (MQ=255)
                               gTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGc                                                                                                                   <  7:336338/149‑1 (MQ=255)
                                tGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCgg                                                                                                                    >  4:200615/1‑147 (MQ=255)
                                   cAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGc                                                                                                               >  3:251625/1‑149 (MQ=255)
                                       ccGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCg                                                                                                           >  6:115352/1‑149 (MQ=255)
                                       ccGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCg                                                                                                           >  3:34989/1‑149 (MQ=255)
                                       ccGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGGCGATATCGACATGGccgtt                                                                                                                                   >  6:226936/1‑122 (MQ=255)
                                       ccGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGGCGATATCGACATGGccgtt                                                                                                                                   <  5:226936/125‑4 (MQ=255)
                                        cgcgCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAg                                                                                                                 <  1:210017/142‑1 (MQ=255)
                                        cgcgCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAg                                                                                                                 >  2:210017/1‑142 (MQ=255)
                                         gcgcTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCg                                                                                                                     <  1:172265/137‑1 (MQ=255)
                                         gcgcTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCg                                                                                                                     >  2:172265/1‑137 (MQ=255)
                                                   aGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCtt                                                                                               <  7:343978/149‑1 (MQ=255)
                                                   aGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCtt                                                                                               <  3:200615/149‑1 (MQ=255)
                                                    gcgcgcGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTg                                                                                              <  6:34336/149‑1 (MQ=255)
                                                     cgcgcgGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGa                                                                                             <  7:247264/149‑1 (MQ=255)
                                                      gcgcgGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAg                                                                                            >  3:283532/1‑149 (MQ=255)
                                                         cggcgTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGc                                                                                          >  3:39028/1‑148 (MQ=255)
                                                          ggcgTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGa                                                                                        <  3:79429/149‑1 (MQ=255)
                                                          ggcgTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGa                                                                                        <  1:368878/149‑1 (MQ=255)
                                                           gcgTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGAt                                                                                       <  4:311864/149‑1 (MQ=255)
                                                           gcgAACCACGTCGACCAGGCGCGCGGGGGACAACGGTTTTTCCATGAAGTCGTCGCCGCCGTAACGCATGCCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGAt                                                                                       <  1:372778/149‑1 (MQ=255)
                                                                ccACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGagc                                                                                  <  5:274908/149‑1 (MQ=255)
                                                                ccACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGag                                                                                   >  2:119935/1‑148 (MQ=255)
                                                                 cACGTCGACCTGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCTTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGTCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAg                                                                                            <  2:357976/138‑1 (MQ=255)
                                                                 cACGTCGACCACGTGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGAGAtcgga                                                                                   >  1:357976/1‑143 (MQ=255)
                                                                        aCCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAg                                                                          >  4:296220/1‑149 (MQ=255)
                                                                           aGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGc                                                                        >  2:207095/1‑148 (MQ=255)
                                                                             gcgcTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCAt                                                                     <  4:251625/149‑1 (MQ=255)
                                                                                       gagaACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCgg                                                           <  5:76026/149‑1 (MQ=255)
                                                                                        agaACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGc                                                          <  4:283532/149‑1 (MQ=255)
                                                                                           aCGCTTTTTCCATGAAGTCGTAGGAGCCGTTACGCATGGCGCCAACCGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGACACTGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAgg                                                       >  8:65712/1‑149 (MQ=255)
                                                                                             ggTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCg                                                     <  5:350019/149‑1 (MQ=255)
                                                                                             ggTTTTTCCATGAAGTCGTAGGCGCCGATACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCCGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCATTACCCGGCAGGCg                                                     >  4:252729/1‑149 (MQ=255)
                                                                                                 tttCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGat                                                  <  8:158489/148‑1 (MQ=255)
                                                                                                   tCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGatat                                                <  5:115352/148‑1 (MQ=255)
                                                                                                   tCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGatat                                                <  7:52373/148‑1 (MQ=255)
                                                                                                      aTGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGa                                                                                        >  7:77836/1‑105 (MQ=255)
                                                                                                      aTGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCt                                            <  4:125188/149‑1 (MQ=255)
                                                                                                       tGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCt                                            >  7:272960/1‑148 (MQ=255)
                                                                                                            tCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTgacga                                       >  2:85496/1‑148 (MQ=255)
                                                                                                              gTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACaa                                    >  8:228383/1‑149 (MQ=255)
                                                                                                              gTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACa                                     <  7:93323/148‑1 (MQ=255)
                                                                                                              ctAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACaa                                    >  3:50990/2‑149 (MQ=255)
                                                                                                                   cgcCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCg                               >  7:185648/1‑149 (MQ=255)
                                                                                                                      cGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCa                            <  1:202339/149‑1 (MQ=255)
                                                                                                                        ttACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCaa                           >  3:242641/1‑148 (MQ=255)
                                                                                                                           cGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAAtc                       >  6:363925/1‑149 (MQ=255)
                                                                                                                              aTGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGACTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAAtcg                      >  2:84759/1‑147 (MQ=255)
                                                                                                                                 gcgcCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGc                  <  4:34989/148‑1 (MQ=255)
                                                                                                                                     cactggCCATGGCGATATCGACATGGCCGGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGCCGATg             >  7:303653/5‑149 (MQ=255)
                                                                                                                                      aaCGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGCCGATGc            <  3:296220/149‑1 (MQ=255)
                                                                                                                                      aaCGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGCCGATGc            <  2:173807/149‑1 (MQ=255)
                                                                                                                                              tGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGCCGATGCGTTCCAgc    <  8:272960/149‑1 (MQ=255)
                                                                                                                                               gTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGCCGATGCGTTCCAgcg   >  5:125921/1‑149 (MQ=255)
                                                                                                                                                tCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGCCGATGCGTTCCAgcgc  >  6:11/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
GCCAGCTGGCGGCGCAGGGCCACCACTTCGCGTGACAGCCCGCGCTGTTCCAGCGCGCGGCGTACCACGTCGACCAGGCGCTCGGGGGAGAACGGTTTTTCCATGAAGTCGTAGGCGCCGTTACGCATGGCGCCAACGGCCATGTCGATATCGCCATGGCCAGTGATCAGCACCACCGGCAGGCTGCGGTCACGGGCCTTGAGGCGATTGAGCAGCTCCAGGCCATCAATACCCGGCAGGCGGATATCGCTGACGACAATGCCGGCAAAATCGTCGCCGATGCGTTCCAGCGC  >  NC_002947/1220893‑1221185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑