Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,224,611 G→T 8.7% L23I (CTC→ATC)  gltK ← glutamate/aspartate ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,224,6110GT 8.7% 156.2 / 3.6 54L23I (CTC→ATC) gltKglutamate/aspartate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (25/24);  new base T (3/2);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGATCACCAGCAGCAGCGGAATCGAGCGGAAGTAGTTGACGTAGGCGCCGGCCAGGTTCGACAGCAGTTTGCTTGACGACAGGCGCATCAGTGCCAGGATGGTGCCCAGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGGGCAGGCAGGGCGGGGATGATTTCGCTGAAATCCATTTCCATTTACTTGCCCCCCACGGAAATCAGGCCAGGCACGGCGACTTTCTTCTCGACCATGCGCATGAGCAGCATCAGGCCCA  >  NC_002947/1224463‑1224754
                                                                                                                                                    |                                                                                                                                               
gTGATCACCAGCAGCAGCGGAATCGAGCGGAAGTAGTTGACGTAGGCGCCGGCCAGGTTCGACAGCAGTTTGCTTGACGACAGGCGCATCAGTGCCAGGATGGTGCCCAGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAg                                                                                                                                                 >  2:115079/1‑149 (MQ=255)
gTGATCACCAGCAGCAGCGGAATCGAGCGGAAGTAGTTGACGTAGGCGCCGGCCAGGTTCGACAGCAGTTTGCTTGACGACAGGCGCATCAGTGCCAGGATGGTGCCCAGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAg                                                                                                                                                 <  6:219303/149‑1 (MQ=255)
    tCACCAGCAGCAGCGGAATCGAGCGGAAGTAGTTGACGTAGGCGCCGGCCAGGTTCGACAGCAGTTTGCTTGACGACAGGCGCATCAGTGCCAGGATGGTGCCCAGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTc                                                                                                                                             <  5:7382/149‑1 (MQ=255)
        cagcagcagCGGAATCGAGCGGAAGTAGTTGACGTAGGCGCCGGCCAGGTTCGACAGCAGTTTGCTTGACGACAGGCGCATCAGTGCCAGGATGGTGCCCAGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATGa                                                                                                                                         <  1:131581/149‑1 (MQ=255)
        cagcagcagCGGAATCGAGCGGAAGTAGTTGACGTAGGCGCCGGCCAGGTTCGACAGCAGTTTGCTTGACGACAGGCGCATCAGTGCCAGGATGGTGCCCAGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATGa                                                                                                                                         >  7:234670/1‑149 (MQ=255)
              cagcGGAATCGAGCGGAAGTAGTTGACGTAGGCGCCGGCCAGGTTCGACAGCAGTTTGCTTGACGACAGGCGCATCAGTGCCAGGATGGTGCCCAGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATGACCATGc                                                                                                                                   >  2:83562/1‑149 (MQ=255)
                      tCGAGCGGAAGTAGTTGACGTAGGCGCCGGCCAGGTTCGACAGCAGTTTGCTTGACGACAGGCGCATCAGTGCCAGGATGGTGGCCCGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCag                                                                                                                           >  2:254641/1‑149 (MQ=255)
                        gAGCGGAAGTAGTTGACGTAGGCGCCGGCCAGGTTCGACAGCAGTTTGCTTGACGACAGGCGCATCAGTGCCAGGATGGTGCCCAGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCaga                                                                                                                          <  1:250950/148‑1 (MQ=255)
                         aGCGGAAGTAGTTGACGTAGGCGCCGGCCAGGTTCGACAGCAGTTTGCTTGACGACAGGCGCATCAGTGCCAGGATGGTGCCCAGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAgg                                                                                                                        <  5:182923/149‑1 (MQ=255)
                             gAAGTAGTTGACGTAGGCGCCGGCCAGGTTCGACAGCAGTTTGCTTGACGACAGGCGCATCAGTGCCAGGATGGTGCCCAGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGggcag                                                                                                                    <  6:263989/149‑1 (MQ=255)
                               agtagtTGACGTAGGCGCCGGCCAGGTTCGACAGCAGTTTGCTTGACGACAGGCGCATCAGTGCCAGGATGGTGCCCAGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGggcagg                                                                                                                   >  4:125133/1‑148 (MQ=255)
                                gtagtTGACGTAGGCGCCGGCCAGGTTCGACAGCAGTTTGCTTGACGACAGGCGCATCAGTGCCAGGATGGTGCCCAGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGggcagg                                                                                                                   >  4:330127/1‑147 (MQ=255)
                                        cGTAGGCGCCGGCCAGGTTCGACAGCAGTTTGCTTGACGACAGGCGCATCAGTGCCAGGATGGTGCCCAGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGGGCAGGCAGGGCgggg                                                                                                         <  1:115079/149‑1 (MQ=255)
                                               gcCGGCCAGGTTCGACAGCAGTTTGCTTGACGACAGGCGCATCAGTGCCAGGATGGTGCCCAGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGggcagg                                                                                                                   >  1:222178/1‑132 (MQ=255)
                                               gcCGGCCAGGTTCGACAGCAGTTTGCTTGACGACAGGCGCATCAGTGCCAGGATGGTGCCCAGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGggcagg                                                                                                                   >  1:216692/1‑132 (MQ=255)
                                               gcCGGCCAGGTTCGACAGCAGTTTGCTTGACGACAGGCGCATCAGTGCCAGGATGGTGCCCAGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGggcagg                                                                                                                   <  2:216692/132‑1 (MQ=255)
                                               gcCGGCCAGGTTCGACAGCAGTTTGCTTGACGACAGGCGCATCAGTGCCAGGATGGTGCCCAGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGggcagg                                                                                                                   <  2:222178/132‑1 (MQ=255)
                                                ccGGCCAGGTTCGACAGCAGTTTGCTTGACGACAGGCTCATCAGTGCCAGGATGGTGCCCAGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGGGCAGGCAGGGCGGGGATGATTTc                                                                                                 <  1:83562/149‑1 (MQ=255)
                                                 cGGCCAGGTTCGACAGCAGTTTGCTTGACGACAGGCGCATCAGTGCCAGGATGGTGCCCAGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGGGCAGGCAGGGCg                                                                                                            <  5:143865/137‑1 (MQ=255)
                                                 cGGCCAGGTTCGACAGCAGTTTGCTTGACGACAGGCGCATCAGTGCCAGGATGGTGCCCAGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGGGCAGGCAGGGCg                                                                                                            >  6:143865/1‑137 (MQ=255)
                                                  ggCCAGGTTCGACAGCAGTTTGCTTGACGACAGGCGCATCAGTGCCAGGATGGTGCCCAGCACGATACAGCCGATGACTCCCATGACCATAAGATTGAGGGTAATGACCATGCCCTCCCAGAGGGCACGCAGGCCGGGGATGATTTCGc                                                                                               >  7:177220/1‑149 (MQ=255)
                                                   gCCAGGTTCGACAGCAGTTTGCTTGACGACAGGCGCATCAGTGCCAGGATGGTGCCCAGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATTACCATGCCCTCCCAGAGGGCAGGCAGGGCGGGGATGAttggga                                                                                               >  8:231743/1‑144 (MQ=255)
                                                      aGGTTCGACAGCAGTTTGCTTGACGACAGGCGCATCAGTGCCAGGATGGTGCCCAGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGGGCAGGCAGGGCGGGGATGATTTCGCTGaa                                                                                           <  5:354886/149‑1 (MQ=255)
                                                             aCAGCAGTTTGCTTGACGACAGGCGCATCAGTGCCAGGATGGTGCCCAGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGGGCAGGCAGGGCGGGGATGATTTCGCTGAAAtccatt                                                                                    >  7:12602/1‑149 (MQ=255)
                                                              cagcagTTTGCTTGACGACAGGCGCATCAGTGCCAGGATGGTGCCCAGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGGGCAGGCAGGGCGGGGATGATTTCAGCGAAAtcatccc                                                                                   <  3:125133/149‑6 (MQ=255)
                                                                  agTTTGCTTGACGACAGGCGCATCAGTGCCAGGATGGTGCCCAGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGGGCAGGCAGGGCGGGGATGATTTCGCTGAAAtccatttccat                                                                               <  8:12602/149‑1 (MQ=255)
                                                                        cTTGACGACAGGCGCATCAGTGCCAGGATGGTGCCCAGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGGGCAGGTAGGGCGGGGATGATTTCGCTGAAATCCATTTCCATTTACtt                                                                         <  3:16187/149‑1 (MQ=255)
                                                                              gacAGGCGCATCAGTGCCAGGATGGTGCCCAGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGGGCAGGCAGGGCGGGGATGATTTCGCTGAAATCCATTTCCATTTACTTGccccc                                                                   >  2:185289/1‑149 (MQ=255)
                                                                                       aTCAGTGCCAGGATGGTGCCCAGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGGGCAGGCAGGGCGGGGATGATTTCGCTGAAATCCATTTCCATTTACTTGCCCCCCACGGAAAt                                                          >  7:177959/1‑149 (MQ=255)
                                                                                            tGCCAGGATGGTGCCCAGCACGATAACGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGATTGCAGGCAGGGCGGGGATGATTTCGCTGAAATCCATTTCCATTAACTTGCCCCCCACGGAAATcaggc                                                     <  8:234670/149‑1 (MQ=255)
                                                                                                aGGATGGTGCCCAGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGGGCAGGCAGGGCGGGGATGATTTCGCTGAAATCCATTTCCATTTACTTGCCCCCCACGGAAATcaggccagg                                                 <  1:185289/149‑1 (MQ=255)
                                                                                                  gATGGTGCCCAGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGGGCAGGCAGGGCGGGGATGATTTCGCTGAAATCCATTTCCATTTAc                                                                           <  3:183843/121‑1 (MQ=255)
                                                                                                  gATGGTGCCCAGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGGGCAGGCAGGGCGGGGATGATTTCGCTGAAATCCATTTCCATTTAc                                                                           >  4:183843/1‑121 (MQ=255)
                                                                                                        gCCCAGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGGGCAGGCAGGGCGGGGATGATTTCGCTGAAATCCATTTCCATTTACTTGCCCCCCACGGAAATCAGGCCAGGCACGGCGa                                         >  7:173842/1‑149 (MQ=255)
                                                                                                          ccAGCACGATACCGCCGATCACTCCCATGACCATCAGTTTGATGGTCATGACCATGCCCTCCCACAGGGCAGGCACGGCGGGGATCATTTCGCTGAAATCCATTTCCATTTACTTGCCCCCCACGGAAATCAGGCCAGGCACGGCGACt                                       >  5:116934/1‑149 (MQ=255)
                                                                                                          ccAGCAAGTTACCGGCCTTTACTACCATTCCCATCACTTTGATGGTAATGTCCATGCCCTACCAGATGGCAGGCACGGCGGGGATGATTTCGCTGAAATCCATTTCCATTTACTTGCCCCCCACGGAAATCAGGCCAGGCACGGCGACt                                       <  7:231743/149‑1 (MQ=255)
                                                                                                           cAGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGGGCAGGCAGGGCGGGGATGATTTCGCTGAAATCCATTTCGATTTACTTGCCCCCCCCGGAAATCAGGCAAGGCACGGCGACtt                                      >  8:197270/1‑149 (MQ=255)
                                                                                                               aCGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGGGCAGGCAGGGCGGGGATGATTTCGCTGAAATCCATTTCCATTTACTTGCCCCCCACGGAAATCAGGCCAGGCACGGCGACTttctt                                  <  3:330127/149‑1 (MQ=255)
                                                                                                               aCGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGGGCAGGCAGGGCGGGGATGATTTCGCTGAAATCCATTTCCATTTACTTGCCCCCCACGGAAATCAGGCCAGGCACGGCGACTttctt                                  <  2:366310/149‑1 (MQ=255)
                                                                                                                        ccgATGACTCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGGGCAGGCAGGGCGGGGTTGATTTCGCTGAAATCCATTTCCATTTACTTGCCCCCCACGGAAATCAGGCCAGGCACGGCGACTTTCTTCTCGACCAt                         >  7:314060/1‑149 (MQ=255)
                                                                                                                        ccgATGACTCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGGGCAGGCAGGGCGGGGATGATTTCGCTGAAATCCATTTCCATTTACTTGCCCCCCACGGAAATCAGGCCAGGCACGGCGACTTTCTTCTCGACCAt                         >  1:185844/1‑149 (MQ=255)
                                                                                                                               cTCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGGGCAGGCAGGGCGGGGATGATTTCGCTGAAATCCATTTCCATTTACTTGCCCCCCACGGAAATCAGGCCAGGCACGGCGACTTTCTTCTCGACCATGCGCAt                   <  1:254641/148‑1 (MQ=255)
                                                                                                                                tCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGGGCAGGCAGGGCGGGGATGATTTCGCTGAAATCCATTTCCATTTACTTGCCCCCCACGGAAATCAGGCCAGGCACGGCGACTTTCTTCTCGACCATGCGCAt                   >  6:27919/1‑147 (MQ=255)
                                                                                                                                tCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGCGCAGGCAGGGCGGGGATGATTTCGCTGAAATCCATTTCCATTTACTTGCCCCCCACGGAAATCAGGCCAGGCACGGCGACTTTCTTCTCGACCATGCGCAt                   >  8:288702/1‑147 (MQ=255)
                                                                                                                                ggtcATGACCCTCAAACTGATGGTCATGACCATGCCCTCCCAGAGGGCAGGCAGGGCGGGgat                                                                                                       >  2:254865/4‑63 (MQ=18)
                                                                                                                                ggtcATGACCCTCAAACTGATGGTCATGACCATGCCCTCCCAGAGGGCAGGCAGGGCGGGgat                                                                                                       <  1:254865/60‑1 (MQ=18)
                                                                                                                                  tcATGACCCTCAAACTGATGGTCATGACCATGCCCTCCCAGAGGGCAGGCAGGGCGGGGATGATTTCGCTGAAATCCATTTCCATTTACTTGCCCCCCACGGAAATCAGGCCAGGCACGGCGACTTTCTTCTCGACCATGCGCATGag                >  3:79443/2‑148 (MQ=255)
                                                                                                                                  ccATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGGGCAGGCAGGGCGGGGATGATTTCGCTGAAATCCATTTCCATTTACTTGCCCCCCACGGAAATCAGGCCAGGCACGGCGACTttcttc                                 >  7:254170/1‑131 (MQ=255)
                                                                                                                                  ccATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGGGCAGGCAGGGCGGGGATGATTTCGCTGAAATCCATTTCCATTTACTTGCCCCCCACGGAAATCAGGCCAGGCACGGCGACTttcttc                                 <  8:254170/131‑1 (MQ=255)
                                                                                                                                   cATGACCATCAGGTTGAGGGTCATGAACATGCCCTCCGAGAGGTCAGGCAGGGCGGGGATGATTTCGCTGAAATCCATTTCCATTTACTTGCCCCCCACGGAAATCAGGCCAGGCAGGGCGACTTTCTTCTCGAGCATGCGCATGagca              <  8:177220/149‑1 (MQ=255)
                                                                                                                                       aCCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGGGCAGGCAGGGCGGGGATGATTTCGCTGAAATCCATTTCCATTTACTTGCCCCCCACGGAAATCAGGCCAGGCACGGCGACTTTCTTCTCGACCATGCGCATGAgcagca           >  8:296487/1‑148 (MQ=255)
                                                                                                                                        ccATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGGGCAGGCAGGGCGGGGATGATTTCGCTGAAATCCATTTCCATTTACTTGCCCCCCACGGAAATCAGGCCAGGCACGGCGACTTTCTTCTCGACCATGCGCATGAGCAGCAt          >  1:86834/1‑148 (MQ=255)
                                                                                                                                           tCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGGGCAGGCAGGGCGGGGATGATTTCGCTGAAATCCATTTCCATTTACTTGCCCCCCACGGAAATCAGGCCAGGCACGGCGACTTTCTTCTCGACCATGCGCATGAGCAGCATCAgg      >  6:55866/1‑149 (MQ=255)
                                                                                                                                               tttGAGGGTCATGACCATGCCCTCCCAGAGGGCAGGCAGGGCGGGGATGATTTCGCTGAAATCCATTTCCATTTACTTGCCCCCCACGGAAATCAGGCCAGGCACGGCGACTTTCTTCTCGACCATGCGCATGAGCAGCATCAGGCCCa  <  7:288702/149‑1 (MQ=255)
                                                                                                                                               tttGAGGGTCATGACCATGCCCTCCCAGAGGGCAGGCAGGGCGGGGATGATTTCGCTGAAATCCATTTCCATTTACTTGCCCCCCACGGAAATCAGGCCAGGCACGGCGACTTTCTTCTCGACCATGCGCATGAGCAGCATCAGGCCCa  <  5:27919/149‑1 (MQ=255)
                                                                                                                                               tttGAGGGTCATGACCATGCCCTCCCAGAGGGCAGGCAGGGCGGGGATGATTTCGCTGAAATCCATTTCCATTTACTTGCCCCCCACGGAAATCAGGCCAGGCACGGCGACTTTCTTCTCGACCATGCGCATGAGCAGCATCAGGCCCa  >  1:96789/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
GTGATCACCAGCAGCAGCGGAATCGAGCGGAAGTAGTTGACGTAGGCGCCGGCCAGGTTCGACAGCAGTTTGCTTGACGACAGGCGCATCAGTGCCAGGATGGTGCCCAGCACGATACCGCCGATGACTCCCATGACCATCAGTTTGAGGGTCATGACCATGCCCTCCCAGAGGGCAGGCAGGGCGGGGATGATTTCGCTGAAATCCATTTCCATTTACTTGCCCCCCACGGAAATCAGGCCAGGCACGGCGACTTTCTTCTCGACCATGCGCATGAGCAGCATCAGGCCCA  >  NC_002947/1224463‑1224754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑