Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,264,624 G→C 8.0% R266G (CGG→GGG)  PP_1106 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,264,6240GC 8.0% 186.5 / 7.0 63R266G (CGG→GGG) PP_1106hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (28/29);  new base C (3/2);  total (31/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01

CTTGTTCGGTGAGGTAGCGCACCAGTACCTCGACCGAGCCGTGAGTGACCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGCCTGGCGCAGCAGTGGATCGTTGCGCGGAATATCGCCTG  >  NC_002947/1264477‑1264770
                                                                                                                                                   |                                                                                                                                                  
cTTGTTCGGTGAGGTAGCGCACCAGTACCTCGACCGAGCCGTGAGTGACCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCcgg                                                                                                                                                   >  5:242037/1‑149 (MQ=255)
   gTTCGGTGAGGTAGCGCACCAGTACCTCGACCGAGCCGTGAGTGACCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCcggcgg                                                                                                                                                >  8:95397/1‑149 (MQ=255)
      cGGTGAGGTAGCGCACCAGTACCTCGACCGAGCCGTGAGTGACCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCggcgggc                                                                                                                                              <  4:320955/148‑1 (MQ=255)
                gcgcACCAGTACCTCGACCGAGCCGTGAGTGACCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTagca                                                                                                                                   <  4:88163/149‑1 (MQ=255)
                   cACCAGTACCTCGACCGAGCCGTGAGTGACCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAgcagca                                                                                                                                >  7:144948/1‑149 (MQ=255)
                    aCCAGTACCTCGACCGAGCCGTGAGTGACCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCAt                                                                                                                               >  7:171140/1‑149 (MQ=255)
                            cTCGACCGAGCCGTGAGTGACCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTagca                                                                                                                                   >  5:23756/1‑137 (MQ=255)
                            cTCGACCGAGCCGTGAGTGACCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTagca                                                                                                                                   <  6:23756/137‑1 (MQ=255)
                            cTCGACCGAGCCGTGAGTGACCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGc                                                                                                                       <  7:24352/149‑1 (MQ=255)
                             tCGACCGAGCCGTGAGTGACCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCt                                                                                                                      >  1:123518/1‑149 (MQ=255)
                                   gAGCCGTGAGTGACCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGaa                                                                                                                <  6:361939/149‑1 (MQ=255)
                                    aGCCGTGAGTGACCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAg                                                                                                               <  2:320703/149‑1 (MQ=255)
                                    aGCCGTGAGTGACCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAg                                                                                                               >  3:169008/1‑149 (MQ=255)
                                        gtgagtgaCCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCAt                                                                                                           <  6:18335/149‑1 (MQ=255)
                                        gtgagtgaCCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCAt                                                                                                           <  5:309814/149‑1 (MQ=255)
                                         tgagtgaCCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATc                                                                                                          <  3:371120/149‑1 (MQ=255)
                                             tgaCCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTg                                                                                                      >  3:348636/1‑149 (MQ=255)
                                             tgaCCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTg                                                                                                      <  2:123518/149‑1 (MQ=255)
                                                ccATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTaa                                                                                                   >  5:282262/1‑149 (MQ=255)
                                                 cATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAAt                                                                                                  >  7:96000/1‑149 (MQ=255)
                                                                aCCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCa                                                                                   >  1:187389/1‑149 (MQ=255)
                                                                        ggCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTa                                                                           >  5:137573/1‑149 (MQ=255)
                                                                         gCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTAc                                                                          >  5:145836/1‑149 (MQ=255)
                                                                           cAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGgc                                                                                      >  3:117051/1‑135 (MQ=255)
                                                                           cAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGgc                                                                                      <  4:117051/135‑1 (MQ=255)
                                                                           cAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTg                                                                        >  2:74202/1‑149 (MQ=255)
                                                                                 cccAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCg                                                                  <  8:96000/149‑1 (MQ=255)
                                                                                    agagCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCt                                                               >  1:214995/1‑149 (MQ=255)
                                                                                          ggccaggccaGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACggg                                                         >  1:189527/1‑149 (MQ=255)
                                                                                           gccaggccagTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCTGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACgggg                                                        <  2:214995/149‑1 (MQ=255)
                                                                                           gccaggccagTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGc                                                                                               <  8:233870/110‑1 (MQ=255)
                                                                                           gccaggccagTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGc                                                                                               <  6:232133/110‑1 (MQ=255)
                                                                                           gccaggccagTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGc                                                                                               >  7:233870/1‑110 (MQ=255)
                                                                                           gccaggccagTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGc                                                                                               >  5:232133/1‑110 (MQ=255)
                                                                                             caggccagTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGgc                                                      <  2:187389/149‑1 (MQ=255)
                                                                                                  cagTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAg                                                 >  4:182563/1‑149 (MQ=255)
                                                                                                  cagTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAg                                                 <  4:169008/149‑1 (MQ=255)
                                                                                                   agTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAgg                                                <  6:282262/149‑1 (MQ=255)
                                                                                                   agTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAg                                                 >  6:152633/1‑148 (MQ=255)
                                                                                                     tCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGAc                                              <  8:144948/149‑1 (MQ=255)
                                                                                                       ggCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCa                                            <  5:152633/149‑1 (MQ=255)
                                                                                                          gTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCCCCGTCAGCCGGTGgcag                                                    <  3:74112/138‑3 (MQ=255)
                                                                                                          gTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCCCCGTCAGCCGGTGgcag                                                    >  4:74112/1‑136 (MQ=255)
                                                                                                          gTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTg                                         <  6:137573/149‑1 (MQ=255)
                                                                                                            ggTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCACCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGcc                                       >  7:335962/1‑149 (MQ=255)
                                                                                                              tCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGCCTg                                     >  5:93174/1‑149 (MQ=255)
                                                                                                                ggACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCGACTGCCACCGGCTGCAGGGGGCGCCAGGACCAGAGCCTGgc                                   >  4:280460/1‑149 (MQ=255)
                                                                                                                 gACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGCCTGgcg                                  <  2:308038/149‑1 (MQ=255)
                                                                                                                  aCAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGCACTGGTCCTGgcgc                                 <  3:182563/149‑1 (MQ=255)
                                                                                                                    aGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGCCTGGCgcag                               >  4:91352/1‑149 (MQ=255)
                                                                                                                    aGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGCCTGGCgcag                               <  3:91352/149‑1 (MQ=255)
                                                                                                                      cacaAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGc                                        <  4:160224/138‑1 (MQ=255)
                                                                                                                      cacaAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGc                                        >  3:160224/1‑138 (MQ=255)
                                                                                                                        caAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGCCTGGCGCAGCAGt                           <  6:242037/149‑1 (MQ=255)
                                                                                                                         aaaGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGCCTGGCGCAGCAGTg                          >  6:321252/1‑149 (MQ=255)
                                                                                                                               ccGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCAt                                                                                                           <  4:364616/62‑1 (MQ=255)
                                                                                                                                 ggTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGCCTGGCGCAGCAGTGGATCGTTg                  <  1:74202/149‑1 (MQ=255)
                                                                                                                                   tCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGCCTGGCGCAGCAGTGGATCGTTgcg                <  7:95397/149‑1 (MQ=255)
                                                                                                                                      aCGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGCCTCGCGCAGCAGTGGATCGTTGcgcg              >  4:330435/1‑148 (MQ=255)
                                                                                                                                       ctacgcggcgcCCGCCGGCGGCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGCCTGGCGcagcag                            >  1:8778/4‑133 (MQ=255)
                                                                                                                                       ctacgcggcgcCCGCCGGCGGCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGCCTGGCGcagcag                            <  2:8778/130‑1 (MQ=255)
                                                                                                                                          cgcgccgcCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGCCTGGCGCAGCAGTGGATCGTTGCGCGGAata         >  7:23678/1‑149 (MQ=255)
                                                                                                                                           gcggcgcCCGCCGGCGGCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGCCGGGGGCGCCAGGACCAGTGCCTGGCGCAGCAGTGGATCGTTGCGCGGAatat        >  1:366299/1‑149 (MQ=255)
                                                                                                                                           gcggcgcCCGCCGGCGGCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGt                                          >  4:17395/1‑115 (MQ=255)
                                                                                                                                           gcggcgcCCGCCGGCGGCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGt                                          <  3:17395/115‑1 (MQ=255)
                                                                                                                                            cgccgcCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGCCTGGCGCAGCAGTGGATCGTTGCGCGGAATATc       <  5:321252/149‑1 (MQ=255)
                                                                                                                                              ccgccgGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGCCTGGCGCAGCAGTGGATCGTTGCGCGGAATATCGc     >  5:195287/1‑149 (MQ=255)
                                                                                                                                                 ccgGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGCCTGGCGCAGCAGTGGATCGTTGCGCGGAATATCGCCTg  <  6:145836/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CTTGTTCGGTGAGGTAGCGCACCAGTACCTCGACCGAGCCGTGAGTGACCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGCCTGGCGCAGCAGTGGATCGTTGCGCGGAATATCGCCTG  >  NC_002947/1264477‑1264770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑