Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,264,627	G→C	7.7%	R265G (CGC→GGC)	PP_1106 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,264,627	0	G	C	7.7%	202.1 / 6.6	65	R265G (CGC→GGC)	PP_1106	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (29/31);  new base C (3/2);  total (32/33)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.33e-01

GTTCGGTGAGGTAGCGCACCAGTACCTCGACCGAGCCGTGAGTGACCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGCCTGGCGCAGCAGTGGATCGTTGCGCGGAATATCGCCTG  >  NC_002947/1264480‑1264770                                                                                                                                                    |                                                                                                                                                gTTCGGTGAGGTAGCGCACCAGTACCTCGACCGAGCCGTGAGTGACCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCcggcgg                                                                                                                                                >  8:95397/1‑149 (MQ=255)    cGGTGAGGTAGCGCACCAGTACCTCGACCGAGCCGTGAGTGACCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCggcgggc                                                                                                                                              <  4:320955/148‑1 (MQ=255)              gcgcACCAGTACCTCGACCGAGCCGTGAGTGACCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTagca                                                                                                                                   <  4:88163/149‑1 (MQ=255)                 cACCAGTACCTCGACCGAGCCGTGAGTGACCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAgcagca                                                                                                                                >  7:144948/1‑149 (MQ=255)                  aCCAGTACCTCGACCGAGCCGTGAGTGACCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCAt                                                                                                                               >  7:171140/1‑149 (MQ=255)                          cTCGACCGAGCCGTGAGTGACCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTagca                                                                                                                                   <  6:23756/137‑1 (MQ=255)                          cTCGACCGAGCCGTGAGTGACCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTagca                                                                                                                                   >  5:23756/1‑137 (MQ=255)                          cTCGACCGAGCCGTGAGTGACCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGc                                                                                                                       <  7:24352/149‑1 (MQ=255)                           tCGACCGAGCCGTGAGTGACCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCt                                                                                                                      >  1:123518/1‑149 (MQ=255)                                 gAGCCGTGAGTGACCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGaa                                                                                                                <  6:361939/149‑1 (MQ=255)                                  aGCCGTGAGTGACCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAg                                                                                                               >  3:169008/1‑149 (MQ=255)                                  aGCCGTGAGTGACCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAg                                                                                                               <  2:320703/149‑1 (MQ=255)                                      gtgagtgaCCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCAt                                                                                                           <  6:18335/149‑1 (MQ=255)                                      gtgagtgaCCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCAt                                                                                                           <  5:309814/149‑1 (MQ=255)                                       tgagtgaCCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATc                                                                                                          <  3:371120/149‑1 (MQ=255)                                           tgaCCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTg                                                                                                      <  2:123518/149‑1 (MQ=255)                                           tgaCCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTg                                                                                                      >  3:348636/1‑149 (MQ=255)                                              ccATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTaa                                                                                                   >  5:282262/1‑149 (MQ=255)                                               cATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAAt                                                                                                  >  7:96000/1‑149 (MQ=255)                                                              aCCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCa                                                                                   >  1:187389/1‑149 (MQ=255)                                                                      ggCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTa                                                                           >  5:137573/1‑149 (MQ=255)                                                                       gCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTAc                                                                          >  5:145836/1‑149 (MQ=255)                                                                         cAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGgc                                                                                      >  3:117051/1‑135 (MQ=255)                                                                         cAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGgc                                                                                      <  4:117051/135‑1 (MQ=255)                                                                         cAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTg                                                                        >  2:74202/1‑149 (MQ=255)                                                                               cccAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCg                                                                  <  8:96000/149‑1 (MQ=255)                                                                                  agagCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCt                                                               >  1:214995/1‑149 (MQ=255)                                                                                        ggccaggccaGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACggg                                                         >  1:189527/1‑149 (MQ=255)                                                                                         gccaggccagTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCTGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACgggg                                                        <  2:214995/149‑1 (MQ=255)                                                                                         gccaggccagTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGc                                                                                               >  7:233870/1‑110 (MQ=255)                                                                                         gccaggccagTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGc                                                                                               <  6:232133/110‑1 (MQ=255)                                                                                         gccaggccagTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGc                                                                                               <  8:233870/110‑1 (MQ=255)                                                                                         gccaggccagTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGc                                                                                               >  5:232133/1‑110 (MQ=255)                                                                                           caggccagTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGgc                                                      <  2:187389/149‑1 (MQ=255)                                                                                                cagTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAg                                                 >  4:182563/1‑149 (MQ=255)                                                                                                cagTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAg                                                 <  4:169008/149‑1 (MQ=255)                                                                                                 agTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAgg                                                <  6:282262/149‑1 (MQ=255)                                                                                                 agTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAg                                                 >  6:152633/1‑148 (MQ=255)                                                                                                   tCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGAc                                              <  8:144948/149‑1 (MQ=255)                                                                                                     ggCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCa                                            <  5:152633/149‑1 (MQ=255)                                                                                                        gTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCCCCGTCAGCCGGTGgcag                                                    <  3:74112/138‑3 (MQ=255)                                                                                                        gTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCCCCGTCAGCCGGTGgcag                                                    >  4:74112/1‑136 (MQ=255)                                                                                                        gTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTg                                         <  6:137573/149‑1 (MQ=255)                                                                                                          ggTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCACCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGcc                                       >  7:335962/1‑149 (MQ=255)                                                                                                            tCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGCCTg                                     >  5:93174/1‑149 (MQ=255)                                                                                                              ggACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCGACTGCCACCGGCTGCAGGGGGCGCCAGGACCAGAGCCTGgc                                   >  4:280460/1‑149 (MQ=255)                                                                                                               gACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGCCTGgcg                                  <  2:308038/149‑1 (MQ=255)                                                                                                                aCAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGCACTGGTCCTGgcgc                                 <  3:182563/149‑1 (MQ=255)                                                                                                                  aGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGCCTGGCgcag                               >  4:91352/1‑149 (MQ=255)                                                                                                                  aGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGCCTGGCgcag                               <  3:91352/149‑1 (MQ=255)                                                                                                                    cacaAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGc                                        >  3:160224/1‑138 (MQ=255)                                                                                                                    cacaAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGc                                        <  4:160224/138‑1 (MQ=255)                                                                                                                      caAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGCCTGGCGCAGCAGt                           <  6:242037/149‑1 (MQ=255)                                                                                                                       aaaGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGCCTGGCGCAGCAGTg                          >  6:321252/1‑149 (MQ=255)                                                                                                                             ccGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCAt                                                                                                           <  4:364616/62‑1 (MQ=255)                                                                                                                               ggTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGCCTGGCGCAGCAGTGGATCGTTg                  <  1:74202/149‑1 (MQ=255)                                                                                                                                 tCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGCCTGGCGCAGCAGTGGATCGTTgcg                <  7:95397/149‑1 (MQ=255)                                                                                                                                    aCGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGCCTCGCGCAGCAGTGGATCGTTGcgcg              >  4:330435/1‑148 (MQ=255)                                                                                                                                     ctacgcggcgcCCGCCGGCGGCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGCCTGGCGcagcag                            >  1:8778/4‑133 (MQ=255)                                                                                                                                     ctacgcggcgcCCGCCGGCGGCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGCCTGGCGcagcag                            <  2:8778/130‑1 (MQ=255)                                                                                                                                        cgcgccgcCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGCCTGGCGCAGCAGTGGATCGTTGCGCGGAata         >  7:23678/1‑149 (MQ=255)                                                                                                                                         gcggcgcCCGCCGGCGGCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGCCGGGGGCGCCAGGACCAGTGCCTGGCGCAGCAGTGGATCGTTGCGCGGAatat        >  1:366299/1‑149 (MQ=255)                                                                                                                                         gcggcgcCCGCCGGCGGCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGt                                          <  3:17395/115‑1 (MQ=255)                                                                                                                                         gcggcgcCCGCCGGCGGCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGt                                          >  4:17395/1‑115 (MQ=255)                                                                                                                                          cgccgcCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGCCTGGCGCAGCAGTGGATCGTTGCGCGGAATATc       <  5:321252/149‑1 (MQ=255)                                                                                                                                            ccgccgGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGCCTGGCGCAGCAGTGGATCGTTGCGCGGAATATCGc     >  5:195287/1‑149 (MQ=255)                                                                                                                                               ccgGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGCCTGGCGCAGCAGTGGATCGTTGCGCGGAATATCGCCTg  <  6:145836/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                GTTCGGTGAGGTAGCGCACCAGTACCTCGACCGAGCCGTGAGTGACCATCACCCGTTGGGCACCGGTCTGGCCAATGGCCCAGAGCAGGCCAGGCCAGTCGGCGTGGTCGGACAGCACAAAGCCCCGGTCCACGCCGCGCCGCCGGCGGGCGCCGCGTAGCAGCATCCAGCCGCTGGCGAAGGCATCACTGTAATCGCCAAAACGGCGCATCCAGCTACTGCCACCGGCTGACGGGGGCGCCAGGACCAGTGCCTGGCGCAGCAGTGGATCGTTGCGCGGAATATCGCCTG  >  NC_002947/1264480‑1264770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑