Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,265,269 G→T 6.1% P51T (CCC→ACC)  PP_1106 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,265,2690GT 6.1% 211.5 / 3.5 65P51T (CCC→ACC) PP_1106hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (29/32);  new base T (2/2);  total (31/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

CTCCAGGCGCACCTGTGCCGAACCCAGCACGTGCCCGGCCGGGTGCAGGCTCAACGTCACGCCGTGGTGCAGCAGGCGCTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGCCCGCTCCACGGGCCGCCAGGGGTCGATGTAGAAATCTCCGGGCGGGCAGTACAGGCCTTCGGGGCGGGCGATGACGAGGT  >  NC_002947/1265123‑1265415
                                                                                                                                                  |                                                                                                                                                  
cTCCAGGCGCACCTGTGCCGAACCCAGCACGTGCCCGGCCGGCTGCAGGCTCAACGTCACGCCGTGGTGCAGCAGGCGCTCACCGTAGGGCAGGGTCTGCACATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGc                                                                                                                                                  >  2:171209/1‑149 (MQ=255)
 tCCAGGCGCACCTGTGCCGAACCCAGCACGTGCCCGGCCGGGTGCAGGCTCAACGTCACGCCGTGGTGCAGCAGGCGCTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCt                                                                                                                                                 >  5:230412/1‑149 (MQ=255)
     ggCGCACCTGTGCCGAACCCAGCACGTGCCCGGCCGGGTGCAGGCTCAACGTCACGCCGTGGTGCAGCAGGCGCTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCg                                                                                                                                             <  5:38321/149‑1 (MQ=255)
     ggCGCACCTGTGCCGAACCCAGCACGTGCCCGGCCGGGTGCAGGCTCAACGTCACGCCGTGGTGCAGCAGGCGCTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCg                                                                                                                                             >  2:139806/1‑149 (MQ=255)
      gcgcACCTGTGCCGAACCCAGCACGTGCCCGGCCGGGTGCAGGCTCAACGTCACGCCGTGGTGCAGCAGGCGCTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCgg                                                                                                                                            <  6:230412/149‑1 (MQ=255)
        gcACCTGTGCCGAACCCAGCACGTGCCCGGCCGGGTGCAGGCTCAACGTCACGCCGTGGTGCAGCAGGCGCTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCAGCCCCGGTGCCGGcattc                                                                                                                                          >  6:346298/1‑145 (MQ=255)
         cACCTGTGCCGAACCCAGCACGTGCCCGGCCGGGTGCAGGCTCAACGTCACGCCGTGGTGCAGCAGGCGCTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCAGCCCCGGTGCCGGcattct                                                                                                                                         <  5:346298/149‑6 (MQ=255)
               tgCCGAACCCAGCACGTGCCCGGCCGGGTGCAGGCTCAACGTCACGCCGTGGTGCAGCAGGCGCTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATa                                                                                                                                     <  7:36684/147‑1 (MQ=255)
                   gAACCCAGCACGTGCCCGGCCGGGTGCAGGCTCAACGTCACGCCGTGGTGCAGCAGGCGCTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGc                                                                                                                                >  6:376386/1‑148 (MQ=255)
                     aCCCAGCACGTGCCCGGCCGGGTGCAGGCTCAACGTCACGCCGTGGTGCAGCAGGCGCTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGACTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCAt                                                                                                                             <  7:336946/149‑1 (MQ=255)
                       ccAGCACGTGCCCGGCCGGGTGCAGGCTCAACGTCACGCCGTGGTGCAGCAGGCGCTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTg                                                                                                                           >  4:19635/1‑149 (MQ=255)
                          gCACGTGCCCGGCCGGGTGCAGGCTCAACGTCACGCCGTGGTGCAGCAGGCGCTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCg                                                                                                                        >  5:98529/1‑149 (MQ=255)
                              gTGCCCGGCCGGGTGCAGGCTCAACGTCACGCCGTGGTGCAGCAGGCGCTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGgtgc                                                                                                                    >  4:99539/1‑149 (MQ=255)
                                 cccGGCCGGGTGCAGGCTCAACGTCACGCCGTGGTGCAGCAGGCGCTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGgtgcgtg                                                                                                                 >  2:199249/1‑149 (MQ=255)
                                  ccggccggGTGCAGGCTCAACGTCACGCCGTGGTGCAGCAGGCGCTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGgtgcgtgc                                                                                                                >  1:194250/1‑149 (MQ=255)
                                  ccggccggGTGCAGGCTCAACGTCACGCCGTGGTGCAGCAGGCGCTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGgtgcgtgc                                                                                                                <  4:326290/149‑1 (MQ=255)
                                   cggccggGTGCAGGCTCAACGTCACGCCGTGGTGCAGCAGGCGCTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGtgcgtgcg                                                                                                               >  4:230401/1‑149 (MQ=255)
                                     gccggGTGCAGGCTCAACGTCACGCCGTGGTGCAGCAGGCGCTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGcgtgcgtg                                                                                                             <  3:19635/149‑1 (MQ=255)
                                              aGGCTCAACGTCACGCCGTGGTGCAGCAGGCGCTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTg                                                                                                    <  2:186300/149‑1 (MQ=255)
                                                gCTCAACGTCACGCCGTGGTGCAGCAGGCGCTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGc                                                                                                  >  2:197821/1‑149 (MQ=255)
                                                gCTCAACGTCACGCCGTGGTGCAGCAGGCGCTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGc                                                                                                  <  5:286334/149‑1 (MQ=255)
                                                gCTCAACGTCACGCCGTGGTGCAGCAGGCGCTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGc                                                                                                  <  8:76519/149‑1 (MQ=255)
                                                  tCAACGTCACGCCGTGGTGCAGCAGGCGCTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCa                                                                                                >  5:201473/1‑149 (MQ=255)
                                                    aaCGTCACGCCGTGGTGCAGCAGGCGCTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATg                                                                                              <  1:139806/149‑1 (MQ=255)
                                                    aaCGTCACGCCGTGGTGCAGCAGGCGCTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATg                                                                                              >  7:331569/1‑149 (MQ=255)
                                                    aaCGTCACGCCGTGGTGCAGCAGGCGCTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATg                                                                                              >  8:309200/1‑149 (MQ=255)
                                                      cGTCACGCCGTGGTGCAGCAGGCGCTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATggg                                                                                            <  3:230401/149‑1 (MQ=255)
                                                       gTCACGCCGTGGTGCAGCAGGCGCTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATAGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATggg                                                                                            >  6:345658/1‑148 (MQ=255)
                                                        tCACGCCGTGGTGCAGCAGGCGCTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTa                                                                                          <  1:293177/149‑1 (MQ=255)
                                                          aCGCCGTGGTGCAGCAGGCGCTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAAt                                                                                        <  5:249834/149‑1 (MQ=255)
                                                           cGCCGTGGTGCAGCAGGCGCTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATg                                                                                       <  3:273179/149‑1 (MQ=255)
                                                                 ggtgCAGCAGGCGCTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGcc                                                                                 >  2:70780/1‑149 (MQ=255)
                                                                        cagGCGCTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGCCCGCTCCa                                                                          >  5:358987/1‑149 (MQ=255)
                                                                           gcgcTCACCGTAGGGCAGTGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGCCCGCTCCACgg                                                                       <  4:304571/149‑1 (MQ=255)
                                                                           gcgcTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGTGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGcc                                                                                 >  3:364012/1‑139 (MQ=255)
                                                                           gcgcTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGTGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGcc                                                                                 <  4:364012/139‑1 (MQ=255)
                                                                           gcgcTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGcc                                                                                 <  4:33959/139‑1 (MQ=255)
                                                                           gcgcTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGcc                                                                                 >  3:33959/1‑139 (MQ=255)
                                                                             gcTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGCCCGCTCCACGGgc                                                                     >  2:232491/1‑149 (MQ=255)
                                                                                   cGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGCCCGCTCCACGGGCCGCCAg                                                               <  6:30672/149‑1 (MQ=255)
                                                                                   cGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGCCCGCTCCACGGGCCGCCAg                                                               >  3:349940/1‑149 (MQ=255)
                                                                                        ggCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATCGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGCCCGCTCCACGGGCCGCCAGGGGTc                                                          <  5:345658/149‑1 (MQ=255)
                                                                                           aGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGCCCGCTCCACGGGCCGCCAGGGGTCGAt                                                       <  5:376386/149‑1 (MQ=255)
                                                                                             ggTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGCCCGCTCCACGGGCCGCCAGGGGTCGATGt                                                     <  6:358987/149‑1 (MQ=255)
                                                                                              gTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGCCCGCTCCACGGGCCGCCAGGGGTCGATGTa                                                    <  3:99539/149‑1 (MQ=255)
                                                                                              gTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGCCCGCTCCACGGGCCGCCAGGGGTCGATGTa                                                    <  1:197821/149‑1 (MQ=255)
                                                                                               tCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGCCCGCTCCACGGGCCGCCAGGGGTCGATGTAg                                                   <  1:70780/149‑1 (MQ=255)
                                                                                                 tGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGCCCGCTCCACGGGCCGCCAGGGGTCGATGTAGa                                                  >  6:322848/1‑148 (MQ=255)
                                                                                                   cAGATTGATGTCCTGGCCGAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGACATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGCCCGCTCCACGGGCCGCCAGGGGTCGATGTAGAAAt                                               >  3:18160/1‑149 (MQ=255)
                                                                                                   cAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGCCCGCTCCACGGGCCGCCAGGGGTCGATGTAGaaa                                                <  5:54354/148‑1 (MQ=255)
                                                                                                        tGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGCCCGCTCCACGGGCCGCCAGGGGTCGATGTAGAAATCTcc                                           <  6:201473/148‑1 (MQ=255)
                                                                                                           tGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATgg                                                                                                                                 >  1:175040/1‑59 (MQ=255)
                                                                                                           tGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATgg                                                                                                                                 <  2:175040/59‑1 (MQ=255)
                                                                                                             tCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGCCCGCTCCACGGGCCGCCAGGGGTCGATGTAGAAATCTCcgggcg                                      <  1:232491/148‑1 (MQ=255)
                                                                                                                tGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGCCCGCTCCACGGGCCGCCAGGGGTCGATGTAGAAATCTCcgggcggg                                    >  5:309487/1‑147 (MQ=255)
                                                                                                                  gCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGCCCGCTCCACGGGCCGCCAGGGGTCGATGTAGAAATCTCCGGGCGGGCAGt                                <  1:199249/149‑1 (MQ=255)
                                                                                                                      aGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGCCCGCTCCACGGGCCGCCAGGGGTCGATGTAGAAATCTCCGGGCGGGCAGTACAg                            >  8:208945/1‑149 (MQ=255)
                                                                                                                         cggcTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGCCCGCTCCACGGGCCGCCAGGGGTCGATGTAGAAATCTCcgggcggg                                    <  6:243279/138‑1 (MQ=255)
                                                                                                                         cggcTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGCCCGCTCCACGGGCCGCCAGGGGTCGATGTAGAAATCTCcgggcggg                                    >  5:243279/1‑138 (MQ=255)
                                                                                                                             tGCGCAGAAAGCCGTGACCGGGGCTGGCGGTCAGATCATGGCCTTTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGCCCGCTCCACGGGCCGCCAGGGGTCGATGTAGAAATCTCCGGGCGGGCAGTACAGGCCTTCg                     <  8:331569/149‑1 (MQ=255)
                                                                                                                               cgcAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGCCCGCTCCACGGGCCGCCAGGGGTCGATGTAGAAATCTCCGGGCGGGCAGTACAGGCCTTCggg                   >  4:12937/1‑149 (MQ=255)
                                                                                                                                gcAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGCCCGCTCCACGGGCCGCCAGGGGTCGATGTAGAAATCTCCGGGCGGGCAGTACAGGCCTTCgggg                  >  5:247357/1‑149 (MQ=255)
                                                                                                                                    aaTGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGCCCGCTCCACGGGCCGCCAGGGGTCGATGTAGAAATCTCCGGGCGGGCAGTACAGGCCTTCggg                   >  4:53931/1‑144 (MQ=255)
                                                                                                                                    aaTGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGCCCGCTCCACGGGCCGCCAGGGGTCGATGTAGAAATCTCCGGGCGGGCAGTACAGGCCTTCggg                   <  3:53931/144‑1 (MQ=255)
                                                                                                                                           gCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGCCCGCTCCACGGGCCGCCAGGGGTCGATGTAGAAATCTCCGGGCGGGCAGTACAGGCCTTCGGGGCGGGCGATGAc       <  6:309487/149‑1 (MQ=255)
                                                                                                                                                ggggCTGGCGGTCAGATAATGGTCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGCCCGCTCCACGGGCCGCCAGGGGTCGATGTAGAAATCTCCGGGCGGGCAGTACAGGCCTTCGGGGCGGGCGATGACGAGGt  <  4:18160/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CTCCAGGCGCACCTGTGCCGAACCCAGCACGTGCCCGGCCGGGTGCAGGCTCAACGTCACGCCGTGGTGCAGCAGGCGCTCACCGTAGGGCAGGGTCTGCAGATTGATGTCCTGGCCCAGGCGGCTGCGCAGAATGCCGGCACCGGGGCTGGCGGTCAGATAATGGCCATTGCCGGTGCGTGCGTGGTCGCCGTGGCCATGGGTAATGACAGCCCGCTCCACGGGCCGCCAGGGGTCGATGTAGAAATCTCCGGGCGGGCAGTACAGGCCTTCGGGGCGGGCGATGACGAGGT  >  NC_002947/1265123‑1265415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑